The Experts below are selected from a list of 174 Experts worldwide ranked by ideXlab platform
Elena Cattaneo - One of the best experts on this subject based on the ideXlab platform.
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proliferation and differentiation of neuronal stem cells regulated by Nerve Growth factor
Nature, 1990Co-Authors: Elena Cattaneo, Ron MckayAbstract:Nerve Growth factor plays an important part in neuron–target interactions in the late embryonic and adult brain. We now report that this Growth factor controls the proliferation of neuronal precursors in a defined culture system of cells derived from the early embryonic brain. Neuronal precursor cells were identified by expression of the intermediate filament protein nestin. These cells proliferate in response to Nerve Growth factor but only after they have been exposed to basic fibroblast Growth factor. On withdrawal of Nerve Growth factor, the proliferative cells differentiate into neurons. Thus, in combination with other Growth factors, Nerve Growth factor regulates the proliferation and terminal differentiation of neuroepithelial stem cells.
Ron Mckay - One of the best experts on this subject based on the ideXlab platform.
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proliferation and differentiation of neuronal stem cells regulated by Nerve Growth factor
Nature, 1990Co-Authors: Elena Cattaneo, Ron MckayAbstract:Nerve Growth factor plays an important part in neuron–target interactions in the late embryonic and adult brain. We now report that this Growth factor controls the proliferation of neuronal precursors in a defined culture system of cells derived from the early embryonic brain. Neuronal precursor cells were identified by expression of the intermediate filament protein nestin. These cells proliferate in response to Nerve Growth factor but only after they have been exposed to basic fibroblast Growth factor. On withdrawal of Nerve Growth factor, the proliferative cells differentiate into neurons. Thus, in combination with other Growth factors, Nerve Growth factor regulates the proliferation and terminal differentiation of neuroepithelial stem cells.
Diptendu Chatterjee - One of the best experts on this subject based on the ideXlab platform.
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coupling of Nerve Growth factor to its receptor inhibition by anti gm3 ganglioside antibody
Cellular and Molecular Neurobiology, 2001Co-Authors: Munmun Chakraborty, Diptendu ChatterjeeAbstract:1. Normal differentiation of PC 12 cells and dorsal root ganglionic neurons in culture need Nerve Growth factor (NGF) for their neurite outGrowth.
Gail K. Adler - One of the best experts on this subject based on the ideXlab platform.
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Commercial mouse and human Nerve Growth factors contain Nerve Growth factor prohormone isoforms
Journal of Neuroscience Methods, 1997Co-Authors: Max Reinshagen, Irmlind Geerling, Jayaraman Lakshmanan, H. Rohm, M.p. Lutz, S. Soinila, Viktor E. Eysselein, Gail K. AdlerAbstract:Abstract Highly sensitive chemiluminescence immunoblot analysis was utilized to examine the purity of mouse 2.5S-, β- and 7S Nerve Growth factors as well as that of recombinant human β-Nerve Growth factor obtained from commercial vendors. Three polyclonal antisera and two monoclonal antibodies to 13 kDa Nerve Growth factor (2.5S NGF and β-NGF) were employed for assessing the purity of each preparation. In addition, polyclonal antisera against two prepro-NGF specific domains were used for immunoblotting analysis to ascertain the identity of high molecular weight Nerve Growth factor immunoreactive proteins as prohormones. Both the mouse and human NGF preparations contained 53 and 60 kDa immunoreactive proteins. Of these, the mouse 60 kDa and the human 53 kDa proteins strongly immunoreacted with both prepro-Nerve Growth factor specific domain antibodies suggesting that they are two NGF prohormone isoforms. In addition, both the mouse and human Nerve Growth factor preparations contained proteins that were immunoreactive to polyclonal antisera and monoclonal antibodies to mouse 2.5S and/or β-NGF. High molecular weight aggregates of prohormones were also observed in mouse and human Nerve Growth factor samples. In summary, none of the ten NGF samples examined were pure as stated. Our study cautions investigators in the field to be aware of the presence of Nerve Growth factor prohomones and other proteins in various mouse and human Nerve Growth factors sold commercially.
Luigi Aloe - One of the best experts on this subject based on the ideXlab platform.
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Nerve Growth factor: basic findings and clinical trials
Biomedical Reviews, 1999Co-Authors: Luigi Aloe, Alessandra MiceraAbstract:The Nerve Growth factor is the first-discovered and best-characterized member of the family of neurotrophins. In the introduction of this article we present a brief biographic view of past and present studies of Rita Levi-Montalcini on Nerve Growth factor: Further, the article focuses on pleiotropic activities of Nerve Growth factor, exerting on various cell types, including cells of nervous, immune and endocrine system. Implications of these actions of Nerve Growth factor in the pathogenesis of neurological diseases, autoimmune-inflammatory diseases, allergic diseases, lymphoproliferative diseases, atherosclerotic cardiovascular disease, and neurotrophic corneal ulcers are outlined. Biomedical Reviews 1999; 10: 3-14.
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topical treatment with Nerve Growth factor for corneal neurotrophic ulcers
The New England Journal of Medicine, 1998Co-Authors: Alessandro Lambiase, Paolo Rama, Stefano Bonini, Giancarlo Caprioglio, Luigi AloeAbstract:Background Corneal neurotrophic ulcers associated with impairment of sensory innervation of the cornea may lead to loss of vision, and there is no effective treatment for these ulcers. We evaluated the effects of Nerve Growth factor in patients with this disorder. Methods Twelve patients (14 eyes) with severe corneal neurotrophic ulcers associated with corneal anesthesia were treated with topical Nerve Growth factor 10 times daily for two days and then 6 times daily until the ulcers healed. Treatment continued for 2 weeks after the ulcers healed, and the patients were then followed for up to 15 months. The evolution of the corneal disease during treatment and follow-up was evaluated by slit-lamp examination, photography, fluorescein-dye testing, and tests of corneal sensitivity and best corrected visual acuity. Results Corneal healing began 2 to 14 days after the initiation of treatment with Nerve Growth factor, and all patients had complete healing of their corneal ulcers after 10 days to 6 weeks of trea...
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Nerve Growth factor, mast cells and arthritis
Biomedical Reviews, 1995Co-Authors: Luigi Aloe, Marco Aurelio Tuveri, Francesco AngelucciAbstract:Nerve Growth factor is a well-characterized neurotrophic protein required for the survival and differentiation of a variety of neuronal cell types both in the peripheral and central nervous systems. Recent studies indicate that Nerve Growth factor also plays a role in cells originating in the immune system, since it is synthesized by cells of immune system lineage and its level increases during inflammatory responses. Moreover, it has been shown that cytokines such as interleukin 1β and tumor necrosis factor-α are potent inducers of Nerve Growth factor secretion. These studies were recently confirmed and extended by demonstrating that cells normally present in inflammatory tissues, such as mast cells and lymphocytes, express Nerve Growth factor receptors and are receptive to the action of Nerve Growth factor. The aim of the present review is to outline the current understanding of mast cells and Nerve Growth factor in autoimmune diseases and particularly in arthritis. Biomedical Reviews 1995; 4: 7-14.
