The Experts below are selected from a list of 16785 Experts worldwide ranked by ideXlab platform

Takayuki Takei - One of the best experts on this subject based on the ideXlab platform.

  • Decellularized liver as a practical scaffold with a vascular Network Template for liver tissue engineering.
    Journal of bioscience and bioengineering, 2012
    Co-Authors: Nana Shirakigawa, Hiroyuki Ijima, Takayuki Takei
    Abstract:

    The construction of a functional liver-tissue equivalent using tissue engineering is a very important goal because the liver is a central organ in the body. However, the construction of functional organ-scale liver tissue is impossible because it requires a high-density blood vessel Network. In this study, we focused on decellularization technology to solve this problem. Decellularized liver tissue with a fine vascular tree Network Template was obtained using Triton X-100. The distance between each vascular structure was less than 1 mm. Endothelialization of the blood vessel Network with human umbilical vein endothelial cells (HUVECs) was successfully performed without any leakage of HUVECs to the outside of the vessel structure. Furthermore, hepatocytes/spheroids could be located around the blood vessel structure. This study indicates that decellularized liver tissue is a potential scaffold for creating a practical liver tissue using tissue engineering technology.

Nana Shirakigawa - One of the best experts on this subject based on the ideXlab platform.

  • Decellularized liver as a practical scaffold with a vascular Network Template for liver tissue engineering.
    Journal of bioscience and bioengineering, 2012
    Co-Authors: Nana Shirakigawa, Hiroyuki Ijima, Takayuki Takei
    Abstract:

    The construction of a functional liver-tissue equivalent using tissue engineering is a very important goal because the liver is a central organ in the body. However, the construction of functional organ-scale liver tissue is impossible because it requires a high-density blood vessel Network. In this study, we focused on decellularization technology to solve this problem. Decellularized liver tissue with a fine vascular tree Network Template was obtained using Triton X-100. The distance between each vascular structure was less than 1 mm. Endothelialization of the blood vessel Network with human umbilical vein endothelial cells (HUVECs) was successfully performed without any leakage of HUVECs to the outside of the vessel structure. Furthermore, hepatocytes/spheroids could be located around the blood vessel structure. This study indicates that decellularized liver tissue is a potential scaffold for creating a practical liver tissue using tissue engineering technology.

Stephen F Badylak - One of the best experts on this subject based on the ideXlab platform.

  • perfusion decellularized skeletal muscle as a three dimensional scaffold with a vascular Network Template
    Biomaterials, 2016
    Co-Authors: Jian Zhang, Neill J Turner, Shi Feng Teng, Wen Yue Cheng, H Zhou, Li Zhang, Qiang Wang, Stephen F Badylak
    Abstract:

    There exists a great need for repair grafts with similar volume to human skeletal muscle that can promote the innate ability of muscle to regenerate following volumetric muscle loss. Perfusion decellularization is an attractive technique for extracellular matrix (ECM) scaffold from intact mammalian organ or tissue which has been successfully used in tissue reconstruction. The perfusion-decellularization of skeletal muscle has been poorly assessed and characterized, but the bioactivity and functional capacity of the obtained perfusion skeletal muscle ECM (pM-ECM) to remodel in vivo is unknown. In the present study, pM-ECM was prepared from porcine rectus abdominis (RA). Perfusion-decellularization of porcine RA effectively removed cellular and nuclear material while retaining the intricate three-dimensional microarchitecture and vasculature Networks of the native RA, and many of the bioactive ECM components and mechanical properties. In vivo, partial-thickness abdominal wall defects in rats repaired with pM-ECM showed improved neovascularization, myogenesis and functional recellularization compared to porcine-derived small intestinal submucosa (SIS). These findings show the biologic potential of RA pM-ECM as a scaffold for supporting site appropriate, tissue reconstruction, and provide a better understanding of the importance maintaining the tissue-specific complex three-dimensional architecture of ECM during decellularization and regeneration.

Hiroyuki Ijima - One of the best experts on this subject based on the ideXlab platform.

  • Decellularized liver as a practical scaffold with a vascular Network Template for liver tissue engineering.
    Journal of bioscience and bioengineering, 2012
    Co-Authors: Nana Shirakigawa, Hiroyuki Ijima, Takayuki Takei
    Abstract:

    The construction of a functional liver-tissue equivalent using tissue engineering is a very important goal because the liver is a central organ in the body. However, the construction of functional organ-scale liver tissue is impossible because it requires a high-density blood vessel Network. In this study, we focused on decellularization technology to solve this problem. Decellularized liver tissue with a fine vascular tree Network Template was obtained using Triton X-100. The distance between each vascular structure was less than 1 mm. Endothelialization of the blood vessel Network with human umbilical vein endothelial cells (HUVECs) was successfully performed without any leakage of HUVECs to the outside of the vessel structure. Furthermore, hepatocytes/spheroids could be located around the blood vessel structure. This study indicates that decellularized liver tissue is a potential scaffold for creating a practical liver tissue using tissue engineering technology.

Suckling John - One of the best experts on this subject based on the ideXlab platform.

  • Intraoperative mapping of executive function using electrocorticography for patients with low-grade gliomas
    'Organisation for Economic Co-Operation and Development (OECD)', 2021
    Co-Authors: Erez Yaara, Assem Moataz, Coelho Pedro, Romero-garcia Rafael, Owen Mallory, Mcdonald Alexa, Woodberry Emma, Morris, Robert C, Price, Stephen J., Suckling John
    Abstract:

    Funder: National Institute for Health Research; doi: http://dx.doi.org/10.13039/501100000272; Grant(s): Clinician Scientist Award 35 (ref: NIHR/CS/009/011)Abstract: Background: Intraoperative functional mapping with direct electrical stimulation during awake surgery for patients with diffuse low-grade glioma has been used in recent years to optimize the balance between surgical resection and quality of life following surgery. Mapping of executive functions is particularly challenging because of their complex nature, with only a handful of reports published so far. Here, we propose the recording of neural activity directly from the surface of the brain using electrocorticography to map executive functions and demonstrate its feasibility and potential utility. Methods: To track a neural signature of executive function, we recorded neural activity using electrocorticography during awake surgery from the frontal cortex of three patients judged to have an appearance of diffuse low-grade glioma. Based on existing functional magnetic resonance imaging (fMRI) evidence from healthy participants for the recruitment of areas associated with executive function with increased task demands, we employed a task difficulty manipulation in two counting tasks performed intraoperatively. Following surgery, the data were extracted and analyzed offline to identify increases in broadband high-gamma power with increased task difficulty, equivalent to fMRI findings, as a signature of activity related to executive function. Results: All three patients performed the tasks well. Data were recorded from five electrode strips, resulting in data from 15 channels overall. Eleven out of the 15 channels (73.3%) showed significant increases in high-gamma power with increased task difficulty, 26.6% of the channels (4/15) showed no change in power, and none of the channels showed power decrease. High-gamma power increases with increased task difficulty were more likely in areas that are within the canonical frontoparietal Network Template. Conclusions: These results are the first step toward developing electrocorticography as a tool for mapping of executive function complementarily to direct electrical stimulation to guide resection. Further studies are required to establish this approach for clinical use

  • Intraoperative mapping of executive function using electrocorticography for patients with low-grade gliomas.
    'Organisation for Economic Co-Operation and Development (OECD)', 2020
    Co-Authors: Erez Yaara, Assem Moataz, Coelho Pedro, Romero-garcia Rafael, Owen Mallory, Mcdonald Alexa, Woodberry Emma, Morris, Robert C, Price Stephen, Suckling John
    Abstract:

    Background: Intraoperative functional mapping with direct electrical stimulation during awake surgery for patients with diffuse low-grade glioma has been used in recent years to optimize the balance between surgical resection and quality of life following surgery. Mapping of executive functions is particularly challenging because their complex nature, with only a handful of reports published so far. Here, we propose recording of neural activity directly from the surface of the brain using electrocorticography to map executive functions and demonstrate its feasibility and potential utility. Methods: To track a neural signature of executive function, we recorded neural activity using electrocorticography during awake surgery from the frontal cortex of three patients judged to have an appearance of diffuse low-grade glioma. Based on existing functional magnetic resonance imaging (fMRI) evidence from healthy participants for the recruitment of areas associated with executive function with increased task demands, we employed a task difficulty manipulation in two counting tasks performed intraoperatively. Following surgery, the data were extracted and analyzed offline to identify increases in broadband high-gamma power with increased task difficulty, equivalent to fMRI findings, as a signature of activity related to executive function. Results: All three patients performed the tasks well. Data were recorded from five electrode strips, resulting in data from 15 channels overall. 73.3% of the channels (11/15) showed significant increases in high-gamma power with increased task difficulty, 26.6% channels (4/15) showed no change in power, and none of the channels showed power decrease. High-gamma power increases with increased task difficulty were more likely in areas that are within the canonical frontoparietal Network Template. Conclusions: These results are the first step towards developing electrocorticography as a tool for mapping of executive function complementarily to direct electrical stimulation to guide resection. Further studies are required to establish this approach for clinical use