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Adriana Maggi - One of the best experts on this subject based on the ideXlab platform.

  • insulin like growth factors activate estrogen receptor to control the growth and differentiation of the human Neuroblastoma Cell Line sk er3
    Molecular Endocrinology, 1994
    Co-Authors: Z Q, Cesare Patrone, Giuseppe Pollio, Sabrina Santagati, Elisabetta Vegeto, Adriana Maggi
    Abstract:

    The Neuroblastoma Cell Line SK-ER3, which is stably transfected with the estrogen receptor (ER), was used to study the effect of insulin and insulin-like growth factors (IGF-I and IGF-II) on growth and morphological differentiation induced by estrogens. The data demonstrate that insulin and related growth factors control the growth and morphological differentiation of the Cell Line expressing the ER, but not of the parental Cell Line. Effects elicited by the growth factors in SK-ER3 Cells can be blocked by ER antagonists. Transient transfection studies further confirm an effect of the IGFs in modulation of ER-activated promoters. The results presented support the hypothesis of the existence of cross-talk between membrane and intraCellular receptors and provide evidence for physiological consequences of the activation of such a pathway of communication. The present study is of particular interest with regard to the theory of prenatal involvement of the ER in maturation of nerve Cells. It could, in fact, be...

  • estrogen modulation of catecholamine synthesis and monoamine oxidase a activity in the human Neuroblastoma Cell Line sk er3
    The Journal of Steroid Biochemistry and Molecular Biology, 1993
    Co-Authors: Z Q, G P Bondiolotti, M Olasmaa, E Violani, Cesare Patrone, G B Picotti, Adriana Maggi
    Abstract:

    Abstract In order to assess the neuronal-like properties of a human Neuroblastoma Cell Line obtained by stable transfection of the estrogen receptor (SK-ER3) a series of quantitative measurements of the activity of two neurotransmitter-related enzymes: tyrosine hydroxylase (TH) and monoamine oxidase (MAO), and of catecholamine concentrations were performed. When compared to the parental SK-N-BE Cell Line, the stably transfected SK-ER3 Cells show a more pronounced dopaminergic phenotype. The immunoreactivity to a TH antibody is in fact increased and the ratio between dopamine and noradrenaLine concentrations is elevated. Treatment with estradiol further enhances the expression of this phenotype. Interestingly, in the transfected Cell Line MAO-A activity is decreased and further reduced by estrogen treatment. This finding substantiated by previous reports indicates that our model system might represent an interesting tool for the study of the pharmacological treatments of estrogen-induced pathological responses of nervous Cells.

  • activated estrogen receptor mediates growth arrest and differentiation of a Neuroblastoma Cell Line
    Proceedings of the National Academy of Sciences of the United States of America, 1993
    Co-Authors: Z Q, E Spreafico, Giuseppe Pollio, Sabrina Santagati, E Conti, Elena Cattaneo, Adriana Maggi
    Abstract:

    Several reports demonstrate estrogen receptor involvement in specific brain functions. In addition, estrogen receptors are expressed at early stages of brain development, suggesting that estrogens or related molecules may play an instructive role in the differentiation of specific brain areas. The lack of model systems in which these phenomena could be studied prompted us to develop a Neuroblastoma Cell Line expressing the estrogen receptor. The Cell Line expresses the hormone receptor at levels compatible with a physiological activity. The activated estrogen receptor is capable of blocking proliferation of the Cells without exerting toxic effects. Following growth arrest, the Cells display a neuron-like morphology and express tau and synaptophysin, two proteins synthesized in differentiating neurons. The Cell Line generated will provide a valuable model system for molecular and biochemical studies of the activity of estrogens in neural-derived Cells.

Z Q - One of the best experts on this subject based on the ideXlab platform.

  • insulin like growth factors activate estrogen receptor to control the growth and differentiation of the human Neuroblastoma Cell Line sk er3
    Molecular Endocrinology, 1994
    Co-Authors: Z Q, Cesare Patrone, Giuseppe Pollio, Sabrina Santagati, Elisabetta Vegeto, Adriana Maggi
    Abstract:

    The Neuroblastoma Cell Line SK-ER3, which is stably transfected with the estrogen receptor (ER), was used to study the effect of insulin and insulin-like growth factors (IGF-I and IGF-II) on growth and morphological differentiation induced by estrogens. The data demonstrate that insulin and related growth factors control the growth and morphological differentiation of the Cell Line expressing the ER, but not of the parental Cell Line. Effects elicited by the growth factors in SK-ER3 Cells can be blocked by ER antagonists. Transient transfection studies further confirm an effect of the IGFs in modulation of ER-activated promoters. The results presented support the hypothesis of the existence of cross-talk between membrane and intraCellular receptors and provide evidence for physiological consequences of the activation of such a pathway of communication. The present study is of particular interest with regard to the theory of prenatal involvement of the ER in maturation of nerve Cells. It could, in fact, be...

  • estrogen modulation of catecholamine synthesis and monoamine oxidase a activity in the human Neuroblastoma Cell Line sk er3
    The Journal of Steroid Biochemistry and Molecular Biology, 1993
    Co-Authors: Z Q, G P Bondiolotti, M Olasmaa, E Violani, Cesare Patrone, G B Picotti, Adriana Maggi
    Abstract:

    Abstract In order to assess the neuronal-like properties of a human Neuroblastoma Cell Line obtained by stable transfection of the estrogen receptor (SK-ER3) a series of quantitative measurements of the activity of two neurotransmitter-related enzymes: tyrosine hydroxylase (TH) and monoamine oxidase (MAO), and of catecholamine concentrations were performed. When compared to the parental SK-N-BE Cell Line, the stably transfected SK-ER3 Cells show a more pronounced dopaminergic phenotype. The immunoreactivity to a TH antibody is in fact increased and the ratio between dopamine and noradrenaLine concentrations is elevated. Treatment with estradiol further enhances the expression of this phenotype. Interestingly, in the transfected Cell Line MAO-A activity is decreased and further reduced by estrogen treatment. This finding substantiated by previous reports indicates that our model system might represent an interesting tool for the study of the pharmacological treatments of estrogen-induced pathological responses of nervous Cells.

  • activated estrogen receptor mediates growth arrest and differentiation of a Neuroblastoma Cell Line
    Proceedings of the National Academy of Sciences of the United States of America, 1993
    Co-Authors: Z Q, E Spreafico, Giuseppe Pollio, Sabrina Santagati, E Conti, Elena Cattaneo, Adriana Maggi
    Abstract:

    Several reports demonstrate estrogen receptor involvement in specific brain functions. In addition, estrogen receptors are expressed at early stages of brain development, suggesting that estrogens or related molecules may play an instructive role in the differentiation of specific brain areas. The lack of model systems in which these phenomena could be studied prompted us to develop a Neuroblastoma Cell Line expressing the estrogen receptor. The Cell Line expresses the hormone receptor at levels compatible with a physiological activity. The activated estrogen receptor is capable of blocking proliferation of the Cells without exerting toxic effects. Following growth arrest, the Cells display a neuron-like morphology and express tau and synaptophysin, two proteins synthesized in differentiating neurons. The Cell Line generated will provide a valuable model system for molecular and biochemical studies of the activity of estrogens in neural-derived Cells.

Sergio Crovella - One of the best experts on this subject based on the ideXlab platform.

  • lovastatin induces apoptosis through the mitochondrial pathway in an undifferentiated sh sy5y Neuroblastoma Cell Line
    Cell Death and Disease, 2013
    Co-Authors: Annalisa Marcuzzi, Paola Maura Tricarico, Elisa Piscianz, Giulio Kleiner, Vecchi L Brumatti, Sergio Crovella
    Abstract:

    Lovastatin induces apoptosis through the mitochondrial pathway in an undifferentiated SH-SY5Y Neuroblastoma Cell Line

  • lovastatin induced apoptosis is modulated by geranylgeraniol in a Neuroblastoma Cell Line
    International Journal of Developmental Neuroscience, 2012
    Co-Authors: Annalisa Marcuzzi, Paola Maura Tricarico, Elisa Piscianz, Valentina Zanin, Josef Vuch, Martina Girardelli, Lorenzo Monasta, Anna Monica Bianco, Sergio Crovella
    Abstract:

    Abstract Mevalonic aciduria (MA), the most severe form of mevalonate kinase deficiency (MKD), is still an orphan drug disease and the pathogenetic mechanisms underlying neuronal dysfunction is still poorly understood. In our study we have investigated the apoptotic mechanism mediated by the exposure of the cultured Neuroblastoma Cell Line, SH-SY5Y, to lovastatin in absence or in presence of the isoprenoid, geranylgeraniol, with the aim of unraveling the pathogenesis of MA. Lovastatin, blocks the mevalonate pathway inhibiting the 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CR), an enzyme of the mevalonate pathway upstream the mevalonate kinase enzyme, reproducing biochemical features similar to those found in MKD. We demonstrate that apoptosis in neuronal lovastatin treated-Cells is induced by the mitochondrial pathway, with caspase-9 as the initiator and caspase-3 as the effector caspase. The presence of geranylgeraniol modulates both the caspase-9 and caspase-3 activity in a dose-dependent way, confirming that this isoprenoid enters the mevalonate pathway, is metabolized and finally is able to by-pass the statin biochemical block reconstituting the mevalonate pathway. According to our findings, it should not be the time course adopted that modulates the apoptotic response but rather the isoprenoid itself. Being aware that our results have been obtained using a biochemical model of MKD, and not Cells from patients with the disease, we believe our findings increase the knowledge of MA pathogenesis, and may possibly contribute to the development of novel therapeutic strategies.

Francesco Clementi - One of the best experts on this subject based on the ideXlab platform.

  • extremely low frequency electromagnetic field elf emf does not affect the expression of α3 α5 and α7 nicotinic receptor subunit genes in sh sy5y Neuroblastoma Cell Line
    Toxicology Letters, 2006
    Co-Authors: Ruth Adele Antonini, Francesco Clementi, Cecilia Gotti, Roberta Benfante, Milena Moretti, Niels Kuster, Juergen Schuderer, Diego Fornasari
    Abstract:

    Abstract Neuronal nicotinic acetylchoLine receptors (nAChRs) are involved in a number of functional processes, including cognition, learning and memory, and alterations in their expression and/or activity have been implicated in various neurological disorders such as Alzheimer's disease (AD), Parkinson's disease and schizophrenia. Epidemiological studies have shown that exposure to electromagnetic fields (EMF) may contribute to the pathogenesis of neurodegenerative diseases such as Alzheimer's disease. Given the role of nAChRs in physiological and pathological conditions, we wondered whether an extremely low-frequency electromagnetic field (ELF-EMF) may affect the expression of the molecules involved in neurodegenerative processes. In order to investigate this possibility, we studied the expression of α3, α5 and α7 nicotinic subunits upon exposure of the SH-SY5Y human Neuroblastoma Cell Line to a 50 Hz power-Line magnetic field in a “blind trial” system; various magnetic flux densities and exposure times were applied. Our studies show that the expression of some relevant components of the choLinergic nicotinic system, which is one of the most affected neurotransmission systems in AD, did not undergo any change at molecular level by environmental exposure to ELF-EMF.

  • Cell plasticity during in vitro differentiation of a human Neuroblastoma Cell Line.
    Advances in experimental medicine and biology, 1991
    Co-Authors: Francesco Clementi, Cecilia Gotti, Emanuele Sher, A. Zanini
    Abstract:

    The differentiation of nervous Cells is a crucial process in the normal development of nervous tissue during ontogenesis, as well as for the regulation of Cell plasticity during Cell growth and regeneration.

Mauro Toselli - One of the best experts on this subject based on the ideXlab platform.

  • functional changes in potassium conductances of the human Neuroblastoma Cell Line sh sy5y during in vitro differentiation
    Journal of Neurophysiology, 1996
    Co-Authors: Patrizia Tosetti, Vanni Taglietti, Mauro Toselli
    Abstract:

    1. The electrophysiological properties of voltage-dependent sodium currents were studied in the human Neuroblastoma Cell Line SH-SY5Y before and after in vitro differentiation with retinoic acid, with the use of the whole Cell variant of the patch-clamp technique. 2. Voltage steps from a holding level of -90 mV to depolarizing potentials elicited, in both undifferentiated and differentiated Cells, fast inward sodium currents that were full inactivating and tetrodotoxin sensitive. 3. In undifferentiated Cells the current peaked at -10 mV, the half-activation potential was -35 mV, and the half-inactivation potential was -81 mV. In differentiated Cells the current peaked at + 10 mV, the half-activation potential was -28 mV, and the half-inactivation potential was -56 mV. Moreover, the peak current amplitude was about a factor of 2 larger and inactivation kinetics was about a factor of 2 slower than in undifferentiated Cells. 4. This diversity in sodium channel properties was related to differences in Cell excitability. Under current-clamp conditions, intraCellular injection of rectangular depolarizing current stimuli from a hyperpolarized membrane potential of about -100 mV elicited graded and weak regenerative responses in undifferentiated Cells, whereas overshooting action potentials with faster rising phases could be elicited in differentiated Cells.