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Elena E Karyakina - One of the best experts on this subject based on the ideXlab platform.

  • equilibrium nad nadh potential on poly Neutral Red modified electrode
    Electrochemistry Communications, 2003
    Co-Authors: Arkady A Karyakin, Yulia N Ivanova, Elena E Karyakina
    Abstract:

    Abstract The electrochemical regeneration of nicotinamide adenine dinucleotide (NAD + /NADH) has been one of the central subjects of bioelectrochemistry during past three decades. We report on the unique chemical electrocatalyst for NAD + /NADH regeneration based on electropolymerized Neutral Red. Using poly(Neutral Red) modified electrodes, the reversible polarographic waves of nicotinamide adenine dinucleotide Reduction–oxidation and the equilibrium (NAD + /NADH) potential were observed. This was impossible using all known catalytic and mediator systems. The unique poly(Neutral Red) based electrocatalyst allowed us to determine the standard (NAD + /NADH) potential more precisely ( E ′ ≅0.59 V SCE, pH 6.0).

  • Equilibrium (NAD+/NADH) potential on poly(Neutral Red) modified electrode
    Electrochemistry Communications, 2003
    Co-Authors: Arkady A Karyakin, Yulia N Ivanova, Elena E Karyakina
    Abstract:

    Abstract The electrochemical regeneration of nicotinamide adenine dinucleotide (NAD + /NADH) has been one of the central subjects of bioelectrochemistry during past three decades. We report on the unique chemical electrocatalyst for NAD + /NADH regeneration based on electropolymerized Neutral Red. Using poly(Neutral Red) modified electrodes, the reversible polarographic waves of nicotinamide adenine dinucleotide Reduction–oxidation and the equilibrium (NAD + /NADH) potential were observed. This was impossible using all known catalytic and mediator systems. The unique poly(Neutral Red) based electrocatalyst allowed us to determine the standard (NAD + /NADH) potential more precisely ( E ′ ≅0.59 V SCE, pH 6.0).

  • equilibrium nad nadh potential on poly Neutral Red modified electrode
    Electrochemistry Communications, 2003
    Co-Authors: Arkady A Karyakin, Yulia N Ivanova, Elena E Karyakina
    Abstract:

    Abstract The electrochemical regeneration of nicotinamide adenine dinucleotide (NAD + /NADH) has been one of the central subjects of bioelectrochemistry during past three decades. We report on the unique chemical electrocatalyst for NAD + /NADH regeneration based on electropolymerized Neutral Red. Using poly(Neutral Red) modified electrodes, the reversible polarographic waves of nicotinamide adenine dinucleotide Reduction–oxidation and the equilibrium (NAD + /NADH) potential were observed. This was impossible using all known catalytic and mediator systems. The unique poly(Neutral Red) based electrocatalyst allowed us to determine the standard (NAD + /NADH) potential more precisely ( E ′ ≅0.59 V SCE, pH 6.0).

Shenming Chen - One of the best experts on this subject based on the ideXlab platform.

  • the electrocatalytic properties of polymerized Neutral Red film modified electrodes
    Journal of Electroanalytical Chemistry, 2001
    Co-Authors: Shenming Chen
    Abstract:

    Polymerization from Neutral Red can be performed in acidic and weakly basic aqueous solution and two types of stable and electrochemically active films can be produced. The film can be produced on glassy carbon, platinum, gold, and transparent semiconductor tin oxide electrodes. A two-layer modified electrode containing organic and inorganic films can also be prepaRed via poly(Neutral Red) and cobalt hexacyanoferrate films; the cobalt(II) hexacyanoferrate film is electrocatalytically active for the oxidation of NADH in 0.1 M RbNO3 aqueous solution and the electrocatalytic oxidation current develops from the anodic peak of the Redox couple. The electrocatalytic Reduction of BrO3 − and IO3 − directly by poly(Neutral Red) in a strong acidic aqueous solution shows that the electrocatalytic Reduction activity is obviously pH dependent in a pH range from 1.0 to 4.0. The iodic electrocatalytic oxidation current appears when I − is produced from the electrocatalytic Reduction of IO3 − by poly(Neutral Red). The catalytic decomposition of H2O2 into H2O and O2 by I − can be initiated via the electrocatalytic Reduction of IO3 − . The electrocatalytic reaction was also performed using the rotating ring-disk electrode method. The polymer film also shows activity towards the electrocatalytic Reduction of oxygen and nitrite in aqueous solution. The electrochemical quartz crystal microbalance technique and cyclic voltammetry were used to study the in situ growth of poly(Neutral Red) film. The polymer films were obtained and transferRed to various solutions between pH 0.5 and 14. The formal potential plotted against pH has a slope − 66 mV/pH. © 2001 Elsevier Science B.V. All rights reserved.

Arkady A Karyakin - One of the best experts on this subject based on the ideXlab platform.

  • equilibrium nad nadh potential on poly Neutral Red modified electrode
    Electrochemistry Communications, 2003
    Co-Authors: Arkady A Karyakin, Yulia N Ivanova, Elena E Karyakina
    Abstract:

    Abstract The electrochemical regeneration of nicotinamide adenine dinucleotide (NAD + /NADH) has been one of the central subjects of bioelectrochemistry during past three decades. We report on the unique chemical electrocatalyst for NAD + /NADH regeneration based on electropolymerized Neutral Red. Using poly(Neutral Red) modified electrodes, the reversible polarographic waves of nicotinamide adenine dinucleotide Reduction–oxidation and the equilibrium (NAD + /NADH) potential were observed. This was impossible using all known catalytic and mediator systems. The unique poly(Neutral Red) based electrocatalyst allowed us to determine the standard (NAD + /NADH) potential more precisely ( E ′ ≅0.59 V SCE, pH 6.0).

  • Equilibrium (NAD+/NADH) potential on poly(Neutral Red) modified electrode
    Electrochemistry Communications, 2003
    Co-Authors: Arkady A Karyakin, Yulia N Ivanova, Elena E Karyakina
    Abstract:

    Abstract The electrochemical regeneration of nicotinamide adenine dinucleotide (NAD + /NADH) has been one of the central subjects of bioelectrochemistry during past three decades. We report on the unique chemical electrocatalyst for NAD + /NADH regeneration based on electropolymerized Neutral Red. Using poly(Neutral Red) modified electrodes, the reversible polarographic waves of nicotinamide adenine dinucleotide Reduction–oxidation and the equilibrium (NAD + /NADH) potential were observed. This was impossible using all known catalytic and mediator systems. The unique poly(Neutral Red) based electrocatalyst allowed us to determine the standard (NAD + /NADH) potential more precisely ( E ′ ≅0.59 V SCE, pH 6.0).

  • equilibrium nad nadh potential on poly Neutral Red modified electrode
    Electrochemistry Communications, 2003
    Co-Authors: Arkady A Karyakin, Yulia N Ivanova, Elena E Karyakina
    Abstract:

    Abstract The electrochemical regeneration of nicotinamide adenine dinucleotide (NAD + /NADH) has been one of the central subjects of bioelectrochemistry during past three decades. We report on the unique chemical electrocatalyst for NAD + /NADH regeneration based on electropolymerized Neutral Red. Using poly(Neutral Red) modified electrodes, the reversible polarographic waves of nicotinamide adenine dinucleotide Reduction–oxidation and the equilibrium (NAD + /NADH) potential were observed. This was impossible using all known catalytic and mediator systems. The unique poly(Neutral Red) based electrocatalyst allowed us to determine the standard (NAD + /NADH) potential more precisely ( E ′ ≅0.59 V SCE, pH 6.0).

Jose Lino Zurita - One of the best experts on this subject based on the ideXlab platform.

  • Neutral Red uptake assay for the estimation of cell viability/cytotoxicity
    Nature Protocols, 2008
    Co-Authors: Giovanni Repetto, Ana Del Peso, Jose Lino Zurita
    Abstract:

    The Neutral Red uptake assay provides a quantitative estimation of the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of viable cells to incorporate and bind the supravital dye Neutral Red in the lysosomes. Most primary cells and cell lines from diverse origin may be successfully used. Cells are seeded in 96-well tissue culture plates and are treated for the appropriate period. The plates are then incubated for 2 h with a medium containing Neutral Red. The cells are subsequently washed, the dye is extracted in each well and the absorbance is read using a spectrophotometer. The procedure is cheaper and more sensitive than other cytotoxicity tests (tetrazolium salts, enzyme leakage or protein content). Once the cells have been treated, the assay can be completed in

  • Neutral Red uptake assay for the estimation of cell viability cytotoxicity
    Nature Protocols, 2008
    Co-Authors: Giovanni Repetto, Ana Del Peso, Jose Lino Zurita
    Abstract:

    The Neutral Red uptake assay provides a quantitative estimation of the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of viable cells to incorporate and bind the supravital dye Neutral Red in the lysosomes. Most primary cells and cell lines from diverse origin may be successfully used. Cells are seeded in 96-well tissue culture plates and are treated for the appropriate period. The plates are then incubated for 2 h with a medium containing Neutral Red. The cells are subsequently washed, the dye is extracted in each well and the absorbance is read using a spectrophotometer. The procedure is cheaper and more sensitive than other cytotoxicity tests (tetrazolium salts, enzyme leakage or protein content). Once the cells have been treated, the assay can be completed in <3 h.

Muhammad A. Rauf - One of the best experts on this subject based on the ideXlab platform.

  • Solvent effect on the spectral properties of Neutral Red
    Chemistry Central Journal, 2008
    Co-Authors: Muhammad A. Rauf, Ahmed A. Soliman, Muhammad Khattab
    Abstract:

    The study was aimed at investigating the effect of various solvents on the absorption spectra of Neutral Red, a dye belonging to the quinone-imine class of dyes. The solvents chosen for the study were water, ethanol, acetonitrile, acetone, propan-1-ol, chloroform, nitrobenzene, ethyleneglycol, acetic acid, DMSO and DMF. The results have shown that the absorption maxima of dyes are dependent on solvent polarity. In non-hydrogen-bond donating solvents, solvation of dye molecules probably occurs via dipole-dipole interactions, whereas in hydrogen-bond donating solvents the phenomenon is more hydrogen bonding in nature. To estimate the contribution of the different variables on the wave number of the Neutral Red dye, regression analyses using the ECW model were compaRed with the π* scale model. This showed that the unified scale for estimating the solvent effect on the absorption of the Neutral Red dye is more adopted and more applicable than the π* scale model. Absorption maxima of dyes are dependent on solvent polarity. Solvation of dye molecules probably occurs via dipole-dipole interactions in non-hydrogen-bond donating solvents, whereas in hydrogen-bond donating solvents the phenomenon is more hydrogen bonding in nature. The unified scale for estimating the solvent effect on the absorption of Neutral Red dye is more adopted and more applicable than the π* scale model. This may be due to complications from both π-π* charge transfer interactions and incomplete complexation of the solute; these effects are averaged out in the derived β and π parameters and thus limit their applicability.

  • Solvent effect on the spectral properties of Neutral Red
    Chemistry Central Journal, 2008
    Co-Authors: Muhammad A. Rauf, Ahmed A. Soliman, Muhammad Khattab
    Abstract:

    The study was aimed at investigating the effect of various solvents on the absorption spectra of Neutral Red, a dye belonging to the quinone-imine class of dyes. The solvents chosen for the study were water, ethanol, acetonitrile, acetone, propan-1-ol, chloroform, nitrobenzene, ethyleneglycol, acetic acid, DMSO and DMF. The results have shown that the absorption maxima of dyes are dependent on solvent polarity. In non-hydrogen-bond donating solvents, solvation of dye molecules probably occurs via dipole-dipole interactions, whereas in hydrogen-bond donating solvents the phenomenon is more hydrogen bonding in nature. To estimate the contribution of the different variables on the wave number of the Neutral Red dye, regression analyses using the ECW model were compaRed with the π* scale model. This showed that the unified scale for estimating the solvent effect on the absorption of the Neutral Red dye is more adopted and more applicable than the π* scale model. Absorption maxima of dyes are dependent on solvent polarity. Solvation of dye molecules probably occurs via dipole-dipole interactions in non-hydrogen-bond donating solvents, whereas in hydrogen-bond donating solvents the phenomenon is more hydrogen bonding in nature. The unified scale for estimating the solvent effect on the absorption of Neutral Red dye is more adopted and more applicable than the π* scale model. This may be due to complications from both π-π* charge transfer interactions and incomplete complexation of the solute; these effects are averaged out in the derived β and π parameters and thus limit their applicability.

  • a comparative study of Neutral Red decoloration by photo fenton and photocatalytic processes
    Dyes and Pigments, 2008
    Co-Authors: Maitha M Alnuaimi, Muhammad A. Rauf, Salman S Ashraf
    Abstract:

    Neutral Red was subjected to two different advanced oxidative processes, namely the photo-Fenton process and the photocatalytic process. The dye was found to undergo substantial and rapid decoloration by both the methods, however, the photo-Fenton-mediated decoloration of the dye was much more efficient. Conditions were optimized in both set of experiments to achieve the most efficient dye decoloration. Additionally the effects of added anions on photocatalytic and photo-Fenton-mediated dye decoloration were examined and compaRed to our previously published results with photolytic and Fenton-mediated Neutral Red decoloration. Most of the ions tested had an inhibitory effect on photo-Fenton process, but unexpectedly enhanced the photocatalytic process, except for chloride, which inhibited both the processes. The kinetics data in both the cases fitted well to the first-order equation. Comparison of the results among the four different advanced oxidation processes shows that Neutral Red is most efficiently degraded by photo-Fenton process, followed by Fenton process, then photolytic, and then with photocatalytic process.