The Experts below are selected from a list of 681 Experts worldwide ranked by ideXlab platform
Marigo Marcelo - One of the best experts on this subject based on the ideXlab platform.
-
Avaliação do perfil imunologico de pacientes sensiveis ao niquel proveniente de aparelhos ortodonticos fixos
[s.n.], 2018Co-Authors: Marigo MarceloAbstract:Orientadores: Darcy Flavio Nouer, Lucia Alves de Oliveira FragaTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: Geralmente, as alergias relatadas na área odontológica, são aquelas de hipersensibilidade tardia a materiais dentários específicos, em particular aqueles que contêm níquel. Reações clínicas a esse metal, proveniente da corrosão dos aparelhos ortodônticos incluem; hipertrofia e sangramento gengival, descamação labial, queilites angulares e eczemas crônicos na mucosa bucal. Normalmente, o método de diagnóstico para a alergia ao níquel, é o teste de sensibilidade cutânea, que pode indicar resultado falso-positivo ou falso-negativo. A proposta deste estudo, foi estabelecer parâmetros imunológicos para diagnóstico diferencial em pacientes sob tratamento ortodôntico e com história clínica de hipersensibilidade ao níquel. Foram realizados testes de proliferação de linfócitos, utilizando como alérgeno, solução contendo níquel proveniente da corrosão dos aparelhos e NiS046H20, e teste ELISA para detectar o envolvimento de subpopulaçôes de Thl e Th2 nas culturas de FBMC. Os resultados mostraram que, a concentração de 10/í g/ml de NiSO4, foi mais efetiva para estimular as células mononucleares do sangue periférico (PBMC) de pacientes com manifestações clínicas bucais, quando comparada com concentrações de 5 e 2,5 £g/ml de NiSO4 Por outro lado, as soluções contendo níquel, proveniente dos aparelhos ortodônticos, inibiram a proliferação de PBMC dos mesmos pacientes, de maneira dose-dependente. Análises estatísticas mostraram uma correlação positiva, entre os indivíduos com manifestações clinicas e o teste de proliferação de PBMC, e verificou-se ainda que quanto maior o tempo de exposição ao aparelho ortodôntico, menor a sensibilidade ao teste cutâneo, independentemente da idade, em indivíduos do sexo feminino. Finalmente, os resultados mostraram que não houve produçãoAbstract: Allergies related to dentistry generally are delayed Hypersensitivity reactions to specific dental materials particularly to Nickel. Clinical reactions to this metal from orthodontic appliances include gingival edema, hypertrophy, bleeding, swollen, fissure lips and chronic eczema of the cheeks. The main diagnostic test for Nickel allergy is the Patch test, which can give false negative results. The purpose of this study was established immunological parameters for differential diagnosis using patients in extensive orthodontic treatment and with clinical history to Nickel Hypersensitivity. We use lymphocyte proliferation test using Nickel leached from orthodontic metal appliances and NtS04.6H20 and ELISA for cytokines on the supernatant of cultured cells to detect the involvement of Thl and Th2 subset The results showed that 10/tg/ml of N1SO4 solution was more effective to stimulate PBMC from patients with oral clinical manifestation when compared with 5 and2.5iug/ml of NiS04 solutions. On the other hand, Nickel solutions obtained from orthodontic metal appliances inhibited the proliferative PBMC response to the same patients in a dose-dependent manner. Statistical analysis showed a positive correlation between persons with clinical manifestation and the PBMC proliferation test. Finally, results showed no 1L-5 and IFN-y production on the supernatant of PBMC cells stimulated with either Nickel solutions in those specific conditions used in our assaysDoutoradoOrtodontiaDoutor em Ciência
-
Vetores cefalometricos representativos do crescimento de maxila e mandibula e suas correlações com a base do cranio
[s.n.], 2018Co-Authors: Marigo MarceloAbstract:Orientador : Everaldo Oliveira Santos BacchiDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: Geralmente, as alergias relatadas na área odontológica, são aquelas de hipersensibilidade tardia a materiais dentários específicos, em particular aqueles que contêm níqueL Reações clínicas a esse Dletal, proveniente da corrosão dos aparelhos ortodônticos incluem; hipertrofia e sangramento gengival, descamação labial, queilites angulares e eczemas crônicos na mucosa bucaL Normalmente, o método de diagnóstico para a alergia ao níquel, é o teste de sensibilidade cutânea, que pode indicar resultado falso-positivo ou falso-negativo. A proposta deste estudo, foi estabelecer parâmetros imunológicos para diagnóstico diferencial em pacientes sob tratamento ortodôntico e. com história clínicadehipersensibilidade ao níqueL Foram realizados testes de proliferação de linfócitos, utilizando cômo alérgeno, solução contendo níquel proveniente da corrosão dos aparelhos e NiS046H2O, e teste ELISA para detectar o envolvimento de subpopulações de Thl e Th2 nas culturas de PBMC. Os resultados mostraram que, a concentração de lO,uglml de NiS04 foi mais efetiva para estimular as células mononucleares do sangue periférico (PBMC) de pacientes com manifestações clínicas bucais, quando comparada com concentrações de 5 e 2,5,uglml de NiS04. Por outro lado, as soluções contendo níquel, proveniente dos aparelhos ortodônticos, inibiram a proliferação de PBMC dos mesmos pacientes, de maneira dose-dependente. Análises estatísticas mostraram uma correlação positiva, entre os indivíduos com manifestações clínicas e o teste de proliferação de PBMC, e verificou-se ainda que quanto maior o tempo de exposição ao aparelho ortodôntico, menor a sensibilidade ao teste cutâneo, i~dependentemente da idade, em indivíduos do sexo feminino. Finalmente, os resultados mostraram que não houve produção significativa de IL-5, nem de IFN-y, nos sobrenadantes das culturas de PBMC, coletados após 24 e 48 horas, nas condições específicas dos ensaios propostosAbstract: Allergies related to dentistry generally are delayed Hypersensitivity reactions to specific dental materiais particularly to Nickel Clinical reactions to this metal from orthodontic appliances include gingival edema, hypertrophy, bleeding, swollen, flSsure lips and chronic eczema of the cheeks. The main diagnostic test for Nickel allergy is the Patch test, which can give false negative results. The purpose of this study was established immunological parameters for differential diagnosis using patients in extensive orthodontic treatment and with clinical history to Nickel Hypersensitivity. We use Iymphocyte proliferation test using Nickel leached from orthodontic metal appliances and NiS04.6H20 and ELISA for cytokines on the supernatant of cultured cells to detect the involvement of Thl and Th2 subset. The results showed that lO,uglml or NiS04 solution was more effective to stimulate PBMC from patients with oral clinical manifestation when compared with 5 and 2.5,uglml of NiS04 solutions. On the other hand, Nickel solutions obtained from orthodontic metal appliances inhibited the proliferative PBMC response to the same patients in a dose-dependent manner. Statistical analysis showed a positive correlation between persons with clinical manifestation and the PBMC proliferation testo Finally, results showed no IL-5 and IFN-y production on the supernatant of PBMC cells stimulated with either Nickel solutions in those specific conditions used in our assaysMestradoOrtodontiaDoutor em Ciência
A. Lahti - One of the best experts on this subject based on the ideXlab platform.
-
Reduced frequency of Nickel allergy upon oral Nickel contact at an early age.
Clinical and experimental immunology, 2008Co-Authors: I. M. W. Hoogstraten, Klaus Ejner Andersen, Georg Kraal, D. P. Bruynzeel, D. Burrows, B. M. E. Blomberg, Dagmar Boden, José G. Camarasa, An Dooms-goossens, A. LahtiAbstract:From animal studies we know that oral administration of T-dependent antigens before sensitization effectively induces systemic immune unresponsiveness. Such 'oral tolerance' is persistent, dose-dependent, antigen-specific and presumably T suppressor cell-mediated. Oral tolerance induction could be an effective way to prevent undesired T cell-mediated immune functions, such as playing a role in allograft reaction, autoimmune and allergic diseases. In the present study allergic contact Hypersensitivity (ACH) to Nickel, currently presenting the most frequent contact allergy in man, was chosen to establish the feasibility of oral prevention of undesired T cell-mediated immunity in man. Potentially tolerizing (oral Nickel contacts via orthodontic braces) as well as sensitizing (ear piercing) events were studied retrospectively in 2176 patients attending nine European patch test clinics. Patients were interviewed by means of a confidential questionnaire. The results show that ear piercing strongly favoured development of Nickel ACH. More importantly, patients having had oral contacts with Nickel-releasing appliances (dental braces) at an early age, but only if prior to ear piercing, showed a reduced frequency of Nickel Hypersensitivity. Frequencies of other hypersensitivities, in particular to fragrance, were not affected. These results support our view that induction of specific systemic immunologic tolerance by timely oral administration of antigens is feasible in man.
-
reduced frequency of Nickel allergy upon oral Nickel contact at an early age
Clinical and Experimental Immunology, 2008Co-Authors: I. M. W. Hoogstraten, Klaus Ejner Andersen, Georg Kraal, D. P. Bruynzeel, D. Burrows, B. M. E. Blomberg, Dagmar Boden, José G. Camarasa, A Doomsgoossens, A. LahtiAbstract:SUMMARY From animal studies we know that oral administration of T-dcpcndcnt antigens before sensitization effectively induces systemic immune unresponsiveness. Such ‘oral tolerance’ is persistent, dose-dependent, antigen-specific and presumably T suppressor cell-mediated. Oral tolerance induction could be an effective way to prevent undesired T cell-mediated immune functions, such as playing a role in allograft reaction, autoimmune and allergic diseases. In the present study allergic contact Hypersensitivity (ACH) to Nickel, currently presenting the most frequent contact allergy in man, was chosen to establish the feasibility of oral prevention of undesired T cell-mediated immunity in man. Potentially tolerizing (oral Nickel contacts via orthodontic braces) as well as sensitizing (ear piercing) events were studied retrospectively in 2176 patients attending nine European patch test clinics. Patients were interviewed by means of a confidential questionnaire. The results show that ear piercing strongly favoured development of Nickel ACH. More importantly, patients having had oral contacts with Nickel-releasing appliances (dental braces) at an early age, but only if prior to ear piercing, showed a reduced frequency of Nickel Hypersensitivity. Frequencies of other hypersensitivities, in particular to fragrance, were not affected. These results support our view that induction of specific systemic immunologic tolerance by timely oral administration of antigens is feasible in man.
Marcelo Marigo - One of the best experts on this subject based on the ideXlab platform.
-
Avaliação do perfil imunologico de pacientes sensiveis ao niquel proveniente de aparelhos ortodonticos fixos
2017Co-Authors: Marcelo MarigoAbstract:Resumo: Geralmente, as alergias relatadas na área odontológica, são aquelas de hipersensibilidade tardia a materiais dentários específicos, em particular aqueles que contêm níquel. Reações clínicas a esse metal, proveniente da corrosão dos aparelhos ortodônticos incluem; hipertrofia e sangramento gengival, descamação labial, queilites angulares e eczemas crônicos na mucosa bucal. Normalmente, o método de diagnóstico para a alergia ao níquel, é o teste de sensibilidade cutânea, que pode indicar resultado falso-positivo ou falso-negativo. A proposta deste estudo, foi estabelecer parâmetros imunológicos para diagnóstico diferencial em pacientes sob tratamento ortodôntico e com história clínica de hipersensibilidade ao níquel. Foram realizados testes de proliferação de linfócitos, utilizando como alérgeno, solução contendo níquel proveniente da corrosão dos aparelhos e NiS046H20, e teste ELISA para detectar o envolvimento de subpopulaçôes de Thl e Th2 nas culturas de FBMC. Os resultados mostraram que, a concentração de 10/í g/ml de NiSO4, foi mais efetiva para estimular as células mononucleares do sangue periférico (PBMC) de pacientes com manifestações clínicas bucais, quando comparada com concentrações de 5 e 2,5 £g/ml de NiSO4 Por outro lado, as soluções contendo níquel, proveniente dos aparelhos ortodônticos, inibiram a proliferação de PBMC dos mesmos pacientes, de maneira dose-dependente. Análises estatísticas mostraram uma correlação positiva, entre os indivíduos com manifestações clinicas e o teste de proliferação de PBMC, e verificou-se ainda que quanto maior o tempo de exposição ao aparelho ortodôntico, menor a sensibilidade ao teste cutâneo, independentemente da idade, em indivíduos do sexo feminino. Finalmente, os resultados mostraram que não houve produçãoAbstract: Allergies related to dentistry generally are delayed Hypersensitivity reactions to specific dental materials particularly to Nickel. Clinical reactions to this metal from orthodontic appliances include gingival edema, hypertrophy, bleeding, swollen, fissure lips and chronic eczema of the cheeks. The main diagnostic test for Nickel allergy is the Patch test, which can give false negative results. The purpose of this study was established immunological parameters for differential diagnosis using patients in extensive orthodontic treatment and with clinical history to Nickel Hypersensitivity. We use lymphocyte proliferation test using Nickel leached from orthodontic metal appliances and NtS04.6H20 and ELISA for cytokines on the supernatant of cultured cells to detect the involvement of Thl and Th2 subset The results showed that 10/tg/ml of N1SO4 solution was more effective to stimulate PBMC from patients with oral clinical manifestation when compared with 5 and2.5iug/ml of NiS04 solutions. On the other hand, Nickel solutions obtained from orthodontic metal appliances inhibited the proliferative PBMC response to the same patients in a dose-dependent manner. Statistical analysis showed a positive correlation between persons with clinical manifestation and the PBMC proliferation test. Finally, results showed no 1L-5 and IFN-y production on the supernatant of PBMC cells stimulated with either Nickel solutions in those specific conditions used in our assay
-
Vetores cefalometricos representativos do crescimento de maxila e mandibula e suas correlações com a base do cranio
2017Co-Authors: Marcelo MarigoAbstract:Resumo: Geralmente, as alergias relatadas na área odontológica, são aquelas de hipersensibilidade tardia a materiais dentários específicos, em particular aqueles que contêm níqueL Reações clínicas a esse Dletal, proveniente da corrosão dos aparelhos ortodônticos incluem; hipertrofia e sangramento gengival, descamação labial, queilites angulares e eczemas crônicos na mucosa bucaL Normalmente, o método dediagnóstico para a alergia ao níquel, é o teste de sensibilidade cutânea, que pode indicar resultado falso-positivo ou falso-negativo. A proposta deste estudo, foi estabelecer parâmetros imunológicos para diagnóstico diferencial em pacientes sob tratamento ortodôntico e. com história clínicadehipersensibilidade ao níqueL Foram realizados testes de proliferação de linfócitos, utilizando cômo alérgeno, soluçãocontendo níquel proveniente da corrosão dos aparelhos e NiS046H2O, e teste ELISA para detectar o envolvimento de subpopulações de Thl e Th2 nas culturas de PBMC. Os resultados mostraram que, a concentração de lO,uglml de NiS04 foi mais efetiva para estimular as células mononucleares do sangue periférico (PBMC) de pacientes com manifestações clínicas bucais, quando comparada com concentrações de 5 e 2,5,uglml de NiS04. Por outro lado, as soluções contendo níquel, proveniente dos aparelhos ortodônticos, inibiram a proliferação de PBMC dos mesmos pacientes, de maneira dose-dependente. Análises estatísticas mostraram uma correlação positiva, entre os indivíduos com manifestações clínicas e o teste de proliferação de PBMC, e verificou-se ainda que quanto maior o tempo de exposição ao aparelho ortodôntico, menor a sensibilidade ao teste cutâneo, i~dependentemente da idade, em indivíduos do sexo feminino. Finalmente, os resultados mostraram que não houve produção significativa de IL-5, nem de IFN-y, nos sobrenadantes das culturas de PBMC, coletados após 24 e 48 horas, nas condições específicas dos ensaios propostosAbstract: Allergies related to dentistry generally are delayed Hypersensitivity reactions to specific dental materiais particularly to Nickel Clinical reactions to this metal from orthodontic appliances include gingival edema, hypertrophy, bleeding, swollen, flSsure lips and chronic eczema of the cheeks. The main diagnostic test for Nickel allergy is the Patch test, which can give false negative results. The purpose of this study was established immunological parameters for differential diagnosis using patients in extensive orthodontic treatment and with clinical history to Nickel Hypersensitivity. We use Iymphocyte proliferation test using Nickel leached from orthodontic metal appliances and NiS04.6H20 and ELISA for cytokines on the supernatant of cultured cells to detect the involvement of Thl and Th2 subset. The results showed thatlO,uglml or NiS04 solution was more effective to stimulate PBMC from patients with oral clinical manifestation when compared with 5 and 2.5,uglml of NiS04 solutions. On the other hand, Nickel solutions obtained from orthodontic metal appliances inhibited the proliferative PBMC response to the same patients in a dose-dependent manner. Statistical analysis showed a positive correlation between persons with clinicalmanifestation and the PBMC proliferation testo Finally, results showed no IL-5 and IFN-y production on the supernatant of PBMC cells stimulated with either Nickel solutions in those specific conditions used in our assay
I. M. W. Hoogstraten - One of the best experts on this subject based on the ideXlab platform.
-
Reduced frequency of Nickel allergy upon oral Nickel contact at an early age.
Clinical and experimental immunology, 2008Co-Authors: I. M. W. Hoogstraten, Klaus Ejner Andersen, Georg Kraal, D. P. Bruynzeel, D. Burrows, B. M. E. Blomberg, Dagmar Boden, José G. Camarasa, An Dooms-goossens, A. LahtiAbstract:From animal studies we know that oral administration of T-dependent antigens before sensitization effectively induces systemic immune unresponsiveness. Such 'oral tolerance' is persistent, dose-dependent, antigen-specific and presumably T suppressor cell-mediated. Oral tolerance induction could be an effective way to prevent undesired T cell-mediated immune functions, such as playing a role in allograft reaction, autoimmune and allergic diseases. In the present study allergic contact Hypersensitivity (ACH) to Nickel, currently presenting the most frequent contact allergy in man, was chosen to establish the feasibility of oral prevention of undesired T cell-mediated immunity in man. Potentially tolerizing (oral Nickel contacts via orthodontic braces) as well as sensitizing (ear piercing) events were studied retrospectively in 2176 patients attending nine European patch test clinics. Patients were interviewed by means of a confidential questionnaire. The results show that ear piercing strongly favoured development of Nickel ACH. More importantly, patients having had oral contacts with Nickel-releasing appliances (dental braces) at an early age, but only if prior to ear piercing, showed a reduced frequency of Nickel Hypersensitivity. Frequencies of other hypersensitivities, in particular to fragrance, were not affected. These results support our view that induction of specific systemic immunologic tolerance by timely oral administration of antigens is feasible in man.
-
reduced frequency of Nickel allergy upon oral Nickel contact at an early age
Clinical and Experimental Immunology, 2008Co-Authors: I. M. W. Hoogstraten, Klaus Ejner Andersen, Georg Kraal, D. P. Bruynzeel, D. Burrows, B. M. E. Blomberg, Dagmar Boden, José G. Camarasa, A Doomsgoossens, A. LahtiAbstract:SUMMARY From animal studies we know that oral administration of T-dcpcndcnt antigens before sensitization effectively induces systemic immune unresponsiveness. Such ‘oral tolerance’ is persistent, dose-dependent, antigen-specific and presumably T suppressor cell-mediated. Oral tolerance induction could be an effective way to prevent undesired T cell-mediated immune functions, such as playing a role in allograft reaction, autoimmune and allergic diseases. In the present study allergic contact Hypersensitivity (ACH) to Nickel, currently presenting the most frequent contact allergy in man, was chosen to establish the feasibility of oral prevention of undesired T cell-mediated immunity in man. Potentially tolerizing (oral Nickel contacts via orthodontic braces) as well as sensitizing (ear piercing) events were studied retrospectively in 2176 patients attending nine European patch test clinics. Patients were interviewed by means of a confidential questionnaire. The results show that ear piercing strongly favoured development of Nickel ACH. More importantly, patients having had oral contacts with Nickel-releasing appliances (dental braces) at an early age, but only if prior to ear piercing, showed a reduced frequency of Nickel Hypersensitivity. Frequencies of other hypersensitivities, in particular to fragrance, were not affected. These results support our view that induction of specific systemic immunologic tolerance by timely oral administration of antigens is feasible in man.
José G. Camarasa - One of the best experts on this subject based on the ideXlab platform.
-
Reduced frequency of Nickel allergy upon oral Nickel contact at an early age.
Clinical and experimental immunology, 2008Co-Authors: I. M. W. Hoogstraten, Klaus Ejner Andersen, Georg Kraal, D. P. Bruynzeel, D. Burrows, B. M. E. Blomberg, Dagmar Boden, José G. Camarasa, An Dooms-goossens, A. LahtiAbstract:From animal studies we know that oral administration of T-dependent antigens before sensitization effectively induces systemic immune unresponsiveness. Such 'oral tolerance' is persistent, dose-dependent, antigen-specific and presumably T suppressor cell-mediated. Oral tolerance induction could be an effective way to prevent undesired T cell-mediated immune functions, such as playing a role in allograft reaction, autoimmune and allergic diseases. In the present study allergic contact Hypersensitivity (ACH) to Nickel, currently presenting the most frequent contact allergy in man, was chosen to establish the feasibility of oral prevention of undesired T cell-mediated immunity in man. Potentially tolerizing (oral Nickel contacts via orthodontic braces) as well as sensitizing (ear piercing) events were studied retrospectively in 2176 patients attending nine European patch test clinics. Patients were interviewed by means of a confidential questionnaire. The results show that ear piercing strongly favoured development of Nickel ACH. More importantly, patients having had oral contacts with Nickel-releasing appliances (dental braces) at an early age, but only if prior to ear piercing, showed a reduced frequency of Nickel Hypersensitivity. Frequencies of other hypersensitivities, in particular to fragrance, were not affected. These results support our view that induction of specific systemic immunologic tolerance by timely oral administration of antigens is feasible in man.
-
reduced frequency of Nickel allergy upon oral Nickel contact at an early age
Clinical and Experimental Immunology, 2008Co-Authors: I. M. W. Hoogstraten, Klaus Ejner Andersen, Georg Kraal, D. P. Bruynzeel, D. Burrows, B. M. E. Blomberg, Dagmar Boden, José G. Camarasa, A Doomsgoossens, A. LahtiAbstract:SUMMARY From animal studies we know that oral administration of T-dcpcndcnt antigens before sensitization effectively induces systemic immune unresponsiveness. Such ‘oral tolerance’ is persistent, dose-dependent, antigen-specific and presumably T suppressor cell-mediated. Oral tolerance induction could be an effective way to prevent undesired T cell-mediated immune functions, such as playing a role in allograft reaction, autoimmune and allergic diseases. In the present study allergic contact Hypersensitivity (ACH) to Nickel, currently presenting the most frequent contact allergy in man, was chosen to establish the feasibility of oral prevention of undesired T cell-mediated immunity in man. Potentially tolerizing (oral Nickel contacts via orthodontic braces) as well as sensitizing (ear piercing) events were studied retrospectively in 2176 patients attending nine European patch test clinics. Patients were interviewed by means of a confidential questionnaire. The results show that ear piercing strongly favoured development of Nickel ACH. More importantly, patients having had oral contacts with Nickel-releasing appliances (dental braces) at an early age, but only if prior to ear piercing, showed a reduced frequency of Nickel Hypersensitivity. Frequencies of other hypersensitivities, in particular to fragrance, were not affected. These results support our view that induction of specific systemic immunologic tolerance by timely oral administration of antigens is feasible in man.