The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Martine Hamann - One of the best experts on this subject based on the ideXlab platform.
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a rapid method combining golgi and Nissl Staining to study neuronal morphology and cytoarchitecture
Journal of Histochemistry and Cytochemistry, 2008Co-Authors: Nadia Pilati, Matthew Barker, Sofoklis Panteleimonitis, Revers Donga, Martine HamannAbstract:The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl Staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their distribution within the tissue slice tend to use the Golgi silver impregnation technique for neuronal morphology followed by deimpregnation as a prerequisite for showing that neuron's histological location by subsequent Nissl Staining. Here, we describe a rapid method combining Golgi silver impregnation with cresyl violet Staining that provides a useful and simple approach to combining cellular morphology with cytoarchitecture without the need for deimpregnating the tissue. Our method allowed us to identify neurons of the facial nucleus and the supratrigeminal nucleus, as well as assessing cellular distribution within layers of the dorsal cochlear nucleus. With this method, we also have been able to directly compare morphological characteristics of neuronal somata at the dorsal cochlear nucleus when labeled with cresyl violet with those obtained with the Golgi method, and we found that cresyl violet-labeled cell bodies appear smaller at high cellular densities. Our observation suggests that cresyl violet Staining is inadequate to quantify differences in soma sizes.
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A rapid method combining Golgi and Nissl Staining to study neuronal morphology and cytoarchitecture.
Journal of Histochemistry and Cytochemistry, 2008Co-Authors: Nadia Pilati, Matthew Barker, Sofoklis Panteleimonitis, Revers Donga, Martine HamannAbstract:SUMMARY The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl Staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their distribution within the tissue slice tend to use the Golgi silver impregnation technique for neuronal morphology followed by deimpregnation as a prerequisite for showing that neuron’s histological location by subsequent Nissl Staining. Here, we describe a rapid method combining Golgi silver impregnation with cresyl violet Staining that provides a useful and simple approach to combining cellular morphology with cytoarchitecture without the need for deimpregnating the tissue. Our method allowed us to identify neurons of the facial nucleus and the supratrigeminal nucleus, as well as assessing cellular distribution within layers of the dorsal cochlear nucleus. With this method, we also have been able to directly compare morphological characteristics of neuronal somata at the dorsal cochlear nucleus when labeled with cresyl violet with those obtained with the Golgi method, and we found that cresyl violet–labeled cell bodies appear smaller at high cellular densities. Our observation suggests that cresyl violet Staining is inadequate to quantify differences in soma sizes. (J Histochem Cytochem 56:539–550, 2008)
Csaba Fekete - One of the best experts on this subject based on the ideXlab platform.
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improved method for combination of immunocytochemistry and Nissl Staining
Journal of Neuroscience Methods, 2009Co-Authors: Andrea Kadar, Gabor Wittmann, Zsolt Liposits, Csaba FeketeAbstract:Abstract Nissl Staining is a widely used method to study morphology and pathology of neural tissue. After standard immunocytochemistry, the Nissl Staining labels only the nucleus of neurons and the characteristic Staining of the neuronal perikarya is absent or very weak. We hypothesized that the RNA degradation during the immunocytochemical treatment results in the loss of cytoplasmic Staining with Nissl-dyes. To test this hypothesis, we used RNAse-free conditions for all steps of immunoStaining. To further prevent the RNA-degradation by RNAse contaminations, the RNAse inhibitor heparin was added to all antibody-containing solutions. The efficiency of Nissl Staining after standard and RNAse-free double-labeling immunocytochemistry was compared using antibodies against c-Fos and neuropeptide Y (NPY) on tissues of rats refed after 3 days of fasting. After standard immunocytochemistry, the Nissl-Staining labeled the nuclei of neurons and only very faintly the cytoplasm of these cells. The RNAse-free treatment did not alter the distribution of immunoreaction signal, but preserved the Staining of neuronal perikarya by the Nissl-dyes. In conclusion, the RNAse-free conditions during immunocytochemistry allow the labeling of neuronal perikarya by Nissl-dyes. The described method facilitates the mapping of immunocytochemical signals and makes possible the light microscopic examination of the innervation of neurons identified by their nuclear protein content.
Nadia Pilati - One of the best experts on this subject based on the ideXlab platform.
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a rapid method combining golgi and Nissl Staining to study neuronal morphology and cytoarchitecture
Journal of Histochemistry and Cytochemistry, 2008Co-Authors: Nadia Pilati, Matthew Barker, Sofoklis Panteleimonitis, Revers Donga, Martine HamannAbstract:The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl Staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their distribution within the tissue slice tend to use the Golgi silver impregnation technique for neuronal morphology followed by deimpregnation as a prerequisite for showing that neuron's histological location by subsequent Nissl Staining. Here, we describe a rapid method combining Golgi silver impregnation with cresyl violet Staining that provides a useful and simple approach to combining cellular morphology with cytoarchitecture without the need for deimpregnating the tissue. Our method allowed us to identify neurons of the facial nucleus and the supratrigeminal nucleus, as well as assessing cellular distribution within layers of the dorsal cochlear nucleus. With this method, we also have been able to directly compare morphological characteristics of neuronal somata at the dorsal cochlear nucleus when labeled with cresyl violet with those obtained with the Golgi method, and we found that cresyl violet-labeled cell bodies appear smaller at high cellular densities. Our observation suggests that cresyl violet Staining is inadequate to quantify differences in soma sizes.
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A rapid method combining Golgi and Nissl Staining to study neuronal morphology and cytoarchitecture.
Journal of Histochemistry and Cytochemistry, 2008Co-Authors: Nadia Pilati, Matthew Barker, Sofoklis Panteleimonitis, Revers Donga, Martine HamannAbstract:SUMMARY The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl Staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their distribution within the tissue slice tend to use the Golgi silver impregnation technique for neuronal morphology followed by deimpregnation as a prerequisite for showing that neuron’s histological location by subsequent Nissl Staining. Here, we describe a rapid method combining Golgi silver impregnation with cresyl violet Staining that provides a useful and simple approach to combining cellular morphology with cytoarchitecture without the need for deimpregnating the tissue. Our method allowed us to identify neurons of the facial nucleus and the supratrigeminal nucleus, as well as assessing cellular distribution within layers of the dorsal cochlear nucleus. With this method, we also have been able to directly compare morphological characteristics of neuronal somata at the dorsal cochlear nucleus when labeled with cresyl violet with those obtained with the Golgi method, and we found that cresyl violet–labeled cell bodies appear smaller at high cellular densities. Our observation suggests that cresyl violet Staining is inadequate to quantify differences in soma sizes. (J Histochem Cytochem 56:539–550, 2008)
Matthew Barker - One of the best experts on this subject based on the ideXlab platform.
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a rapid method combining golgi and Nissl Staining to study neuronal morphology and cytoarchitecture
Journal of Histochemistry and Cytochemistry, 2008Co-Authors: Nadia Pilati, Matthew Barker, Sofoklis Panteleimonitis, Revers Donga, Martine HamannAbstract:The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl Staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their distribution within the tissue slice tend to use the Golgi silver impregnation technique for neuronal morphology followed by deimpregnation as a prerequisite for showing that neuron's histological location by subsequent Nissl Staining. Here, we describe a rapid method combining Golgi silver impregnation with cresyl violet Staining that provides a useful and simple approach to combining cellular morphology with cytoarchitecture without the need for deimpregnating the tissue. Our method allowed us to identify neurons of the facial nucleus and the supratrigeminal nucleus, as well as assessing cellular distribution within layers of the dorsal cochlear nucleus. With this method, we also have been able to directly compare morphological characteristics of neuronal somata at the dorsal cochlear nucleus when labeled with cresyl violet with those obtained with the Golgi method, and we found that cresyl violet-labeled cell bodies appear smaller at high cellular densities. Our observation suggests that cresyl violet Staining is inadequate to quantify differences in soma sizes.
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A rapid method combining Golgi and Nissl Staining to study neuronal morphology and cytoarchitecture.
Journal of Histochemistry and Cytochemistry, 2008Co-Authors: Nadia Pilati, Matthew Barker, Sofoklis Panteleimonitis, Revers Donga, Martine HamannAbstract:SUMMARY The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl Staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their distribution within the tissue slice tend to use the Golgi silver impregnation technique for neuronal morphology followed by deimpregnation as a prerequisite for showing that neuron’s histological location by subsequent Nissl Staining. Here, we describe a rapid method combining Golgi silver impregnation with cresyl violet Staining that provides a useful and simple approach to combining cellular morphology with cytoarchitecture without the need for deimpregnating the tissue. Our method allowed us to identify neurons of the facial nucleus and the supratrigeminal nucleus, as well as assessing cellular distribution within layers of the dorsal cochlear nucleus. With this method, we also have been able to directly compare morphological characteristics of neuronal somata at the dorsal cochlear nucleus when labeled with cresyl violet with those obtained with the Golgi method, and we found that cresyl violet–labeled cell bodies appear smaller at high cellular densities. Our observation suggests that cresyl violet Staining is inadequate to quantify differences in soma sizes. (J Histochem Cytochem 56:539–550, 2008)
Sofoklis Panteleimonitis - One of the best experts on this subject based on the ideXlab platform.
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a rapid method combining golgi and Nissl Staining to study neuronal morphology and cytoarchitecture
Journal of Histochemistry and Cytochemistry, 2008Co-Authors: Nadia Pilati, Matthew Barker, Sofoklis Panteleimonitis, Revers Donga, Martine HamannAbstract:The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl Staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their distribution within the tissue slice tend to use the Golgi silver impregnation technique for neuronal morphology followed by deimpregnation as a prerequisite for showing that neuron's histological location by subsequent Nissl Staining. Here, we describe a rapid method combining Golgi silver impregnation with cresyl violet Staining that provides a useful and simple approach to combining cellular morphology with cytoarchitecture without the need for deimpregnating the tissue. Our method allowed us to identify neurons of the facial nucleus and the supratrigeminal nucleus, as well as assessing cellular distribution within layers of the dorsal cochlear nucleus. With this method, we also have been able to directly compare morphological characteristics of neuronal somata at the dorsal cochlear nucleus when labeled with cresyl violet with those obtained with the Golgi method, and we found that cresyl violet-labeled cell bodies appear smaller at high cellular densities. Our observation suggests that cresyl violet Staining is inadequate to quantify differences in soma sizes.
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A rapid method combining Golgi and Nissl Staining to study neuronal morphology and cytoarchitecture.
Journal of Histochemistry and Cytochemistry, 2008Co-Authors: Nadia Pilati, Matthew Barker, Sofoklis Panteleimonitis, Revers Donga, Martine HamannAbstract:SUMMARY The Golgi silver impregnation technique gives detailed information on neuronal morphology of the few neurons it labels, whereas the majority remain unstained. In contrast, the Nissl Staining technique allows for consistent labeling of the whole neuronal population but gives very limited information on neuronal morphology. Most studies characterizing neuronal cell types in the context of their distribution within the tissue slice tend to use the Golgi silver impregnation technique for neuronal morphology followed by deimpregnation as a prerequisite for showing that neuron’s histological location by subsequent Nissl Staining. Here, we describe a rapid method combining Golgi silver impregnation with cresyl violet Staining that provides a useful and simple approach to combining cellular morphology with cytoarchitecture without the need for deimpregnating the tissue. Our method allowed us to identify neurons of the facial nucleus and the supratrigeminal nucleus, as well as assessing cellular distribution within layers of the dorsal cochlear nucleus. With this method, we also have been able to directly compare morphological characteristics of neuronal somata at the dorsal cochlear nucleus when labeled with cresyl violet with those obtained with the Golgi method, and we found that cresyl violet–labeled cell bodies appear smaller at high cellular densities. Our observation suggests that cresyl violet Staining is inadequate to quantify differences in soma sizes. (J Histochem Cytochem 56:539–550, 2008)