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Sudha Nair - One of the best experts on this subject based on the ideXlab platform.

  • Vibrio mangrovi sp. nov., a diazotrophic Bacterium isolated from mangrove‐associated wild rice (Poteresia coarctata Tateoka)
    Fems Microbiology Letters, 2010
    Co-Authors: Natarajan Rameshkumar, Elke Lang, Cathrin Spröer, Sudha Nair
    Abstract:

    The taxonomic status of a Nitrogen-Fixing Bacterium, strain MSSRF38T, isolated from the rhizosphere of mangrove-associated wild rice ( Porteresia coarctata Tateoka), in Pichavaram, India, was studied using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the novel strain MSSRF38T was most closely related to Vibrio ruber DSM 16370T (98.3% gene sequence similarity), Vibrio rhizosphaerae DSM 18581T (98.2% sequence similarity) and

  • mangrovibacter plantisponsor gen nov sp nov a nitrogen fixing Bacterium isolated from a mangrove associated wild rice porteresia coarctata tateoka
    International Journal of Systematic and Evolutionary Microbiology, 2010
    Co-Authors: Natarajan Rameshkumar, Elke Lang, Sudha Nair
    Abstract:

    A facultatively anaerobic, Nitrogen-Fixing Bacterium, strain MSSRF40T, was isolated from roots of mangrove-associated wild rice (Porteresia coarctata Tateoka). On the basis of 16S rRNA gene sequence similarities, strain MSSRF40T was shown to belong to the family Enterobacteriaceae, most closely related to Cronobacter muytjensii E603T (97.2 % sequence similarity), Enterobacter cloacae subsp. dissolvens LMG 2683T (97.1 %), E. radicincitans D5/23T (97.1 %) and E. ludwigii EN-119T (97.0 %). Sequence analysis of rpoB, gyrB and hsp60 genes showed that strain MSSRF40T had relatively low sequence similarity (<91, <84 and <90 %) to recognized species of different genera of the family Enterobacteriaceae and formed an independent phyletic lineage in all phylogenetic analyses using the 16S rRNA, rpoB, gyrB and hsp60 genes, clearly indicating that strain MSSRF40T could not be affiliated to any of the recognized genera within the family Enterobacteriaceae. The dominant cellular fatty acids were C16 : 0, C16 : 1 ω7c and/or iso-C15 : 0 2-OH and C18 : 1 ω7c, similar to those of other members of the Enterobacteriaceae. The DNA G+C content was 50.1 mol%. Phylogenetic distinctiveness and phenotypic differences from its phylogenetic neighbours indicated that strain MSSRF40T represents a novel species and genus within the family Enterobacteriaceae, for which the name Mangrovibacter plantisponsor gen. nov., sp. nov. is proposed. The type strain of Mangrovibacter plantisponsor is strain MSSRF40T (=LMG 24236T =DSM 19579T).

  • Mangrovibacter plantisponsor gen. nov., sp. nov., a Nitrogen-Fixing Bacterium isolated from a mangrove-associated wild rice (Porteresia coarctata Tateoka).
    International journal of systematic and evolutionary microbiology, 2009
    Co-Authors: Natarajan Rameshkumar, Elke Lang, Sudha Nair
    Abstract:

    A facultatively anaerobic, Nitrogen-Fixing Bacterium, strain MSSRF40(T), was isolated from roots of mangrove-associated wild rice (Porteresia coarctata Tateoka). On the basis of 16S rRNA gene sequence similarities, strain MSSRF40(T) was shown to belong to the family Enterobacteriaceae, most closely related to Cronobacter muytjensii E603(T) (97.2 % sequence similarity), Enterobacter cloacae subsp. dissolvens LMG 2683(T) (97.1 %), E. radicincitans D5/23(T) (97.1 %) and E. ludwigii EN-119(T) (97.0 %). Sequence analysis of rpoB, gyrB and hsp60 genes showed that strain MSSRF40(T) had relatively low sequence similarity (

Natarajan Rameshkumar - One of the best experts on this subject based on the ideXlab platform.

  • Vibrio mangrovi sp. nov., a diazotrophic Bacterium isolated from mangrove‐associated wild rice (Poteresia coarctata Tateoka)
    Fems Microbiology Letters, 2010
    Co-Authors: Natarajan Rameshkumar, Elke Lang, Cathrin Spröer, Sudha Nair
    Abstract:

    The taxonomic status of a Nitrogen-Fixing Bacterium, strain MSSRF38T, isolated from the rhizosphere of mangrove-associated wild rice ( Porteresia coarctata Tateoka), in Pichavaram, India, was studied using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the novel strain MSSRF38T was most closely related to Vibrio ruber DSM 16370T (98.3% gene sequence similarity), Vibrio rhizosphaerae DSM 18581T (98.2% sequence similarity) and

  • mangrovibacter plantisponsor gen nov sp nov a nitrogen fixing Bacterium isolated from a mangrove associated wild rice porteresia coarctata tateoka
    International Journal of Systematic and Evolutionary Microbiology, 2010
    Co-Authors: Natarajan Rameshkumar, Elke Lang, Sudha Nair
    Abstract:

    A facultatively anaerobic, Nitrogen-Fixing Bacterium, strain MSSRF40T, was isolated from roots of mangrove-associated wild rice (Porteresia coarctata Tateoka). On the basis of 16S rRNA gene sequence similarities, strain MSSRF40T was shown to belong to the family Enterobacteriaceae, most closely related to Cronobacter muytjensii E603T (97.2 % sequence similarity), Enterobacter cloacae subsp. dissolvens LMG 2683T (97.1 %), E. radicincitans D5/23T (97.1 %) and E. ludwigii EN-119T (97.0 %). Sequence analysis of rpoB, gyrB and hsp60 genes showed that strain MSSRF40T had relatively low sequence similarity (<91, <84 and <90 %) to recognized species of different genera of the family Enterobacteriaceae and formed an independent phyletic lineage in all phylogenetic analyses using the 16S rRNA, rpoB, gyrB and hsp60 genes, clearly indicating that strain MSSRF40T could not be affiliated to any of the recognized genera within the family Enterobacteriaceae. The dominant cellular fatty acids were C16 : 0, C16 : 1 ω7c and/or iso-C15 : 0 2-OH and C18 : 1 ω7c, similar to those of other members of the Enterobacteriaceae. The DNA G+C content was 50.1 mol%. Phylogenetic distinctiveness and phenotypic differences from its phylogenetic neighbours indicated that strain MSSRF40T represents a novel species and genus within the family Enterobacteriaceae, for which the name Mangrovibacter plantisponsor gen. nov., sp. nov. is proposed. The type strain of Mangrovibacter plantisponsor is strain MSSRF40T (=LMG 24236T =DSM 19579T).

  • Mangrovibacter plantisponsor gen. nov., sp. nov., a Nitrogen-Fixing Bacterium isolated from a mangrove-associated wild rice (Porteresia coarctata Tateoka).
    International journal of systematic and evolutionary microbiology, 2009
    Co-Authors: Natarajan Rameshkumar, Elke Lang, Sudha Nair
    Abstract:

    A facultatively anaerobic, Nitrogen-Fixing Bacterium, strain MSSRF40(T), was isolated from roots of mangrove-associated wild rice (Porteresia coarctata Tateoka). On the basis of 16S rRNA gene sequence similarities, strain MSSRF40(T) was shown to belong to the family Enterobacteriaceae, most closely related to Cronobacter muytjensii E603(T) (97.2 % sequence similarity), Enterobacter cloacae subsp. dissolvens LMG 2683(T) (97.1 %), E. radicincitans D5/23(T) (97.1 %) and E. ludwigii EN-119(T) (97.0 %). Sequence analysis of rpoB, gyrB and hsp60 genes showed that strain MSSRF40(T) had relatively low sequence similarity (

Elke Lang - One of the best experts on this subject based on the ideXlab platform.

  • Vibrio mangrovi sp. nov., a diazotrophic Bacterium isolated from mangrove‐associated wild rice (Poteresia coarctata Tateoka)
    Fems Microbiology Letters, 2010
    Co-Authors: Natarajan Rameshkumar, Elke Lang, Cathrin Spröer, Sudha Nair
    Abstract:

    The taxonomic status of a Nitrogen-Fixing Bacterium, strain MSSRF38T, isolated from the rhizosphere of mangrove-associated wild rice ( Porteresia coarctata Tateoka), in Pichavaram, India, was studied using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the novel strain MSSRF38T was most closely related to Vibrio ruber DSM 16370T (98.3% gene sequence similarity), Vibrio rhizosphaerae DSM 18581T (98.2% sequence similarity) and

  • mangrovibacter plantisponsor gen nov sp nov a nitrogen fixing Bacterium isolated from a mangrove associated wild rice porteresia coarctata tateoka
    International Journal of Systematic and Evolutionary Microbiology, 2010
    Co-Authors: Natarajan Rameshkumar, Elke Lang, Sudha Nair
    Abstract:

    A facultatively anaerobic, Nitrogen-Fixing Bacterium, strain MSSRF40T, was isolated from roots of mangrove-associated wild rice (Porteresia coarctata Tateoka). On the basis of 16S rRNA gene sequence similarities, strain MSSRF40T was shown to belong to the family Enterobacteriaceae, most closely related to Cronobacter muytjensii E603T (97.2 % sequence similarity), Enterobacter cloacae subsp. dissolvens LMG 2683T (97.1 %), E. radicincitans D5/23T (97.1 %) and E. ludwigii EN-119T (97.0 %). Sequence analysis of rpoB, gyrB and hsp60 genes showed that strain MSSRF40T had relatively low sequence similarity (<91, <84 and <90 %) to recognized species of different genera of the family Enterobacteriaceae and formed an independent phyletic lineage in all phylogenetic analyses using the 16S rRNA, rpoB, gyrB and hsp60 genes, clearly indicating that strain MSSRF40T could not be affiliated to any of the recognized genera within the family Enterobacteriaceae. The dominant cellular fatty acids were C16 : 0, C16 : 1 ω7c and/or iso-C15 : 0 2-OH and C18 : 1 ω7c, similar to those of other members of the Enterobacteriaceae. The DNA G+C content was 50.1 mol%. Phylogenetic distinctiveness and phenotypic differences from its phylogenetic neighbours indicated that strain MSSRF40T represents a novel species and genus within the family Enterobacteriaceae, for which the name Mangrovibacter plantisponsor gen. nov., sp. nov. is proposed. The type strain of Mangrovibacter plantisponsor is strain MSSRF40T (=LMG 24236T =DSM 19579T).

  • Mangrovibacter plantisponsor gen. nov., sp. nov., a Nitrogen-Fixing Bacterium isolated from a mangrove-associated wild rice (Porteresia coarctata Tateoka).
    International journal of systematic and evolutionary microbiology, 2009
    Co-Authors: Natarajan Rameshkumar, Elke Lang, Sudha Nair
    Abstract:

    A facultatively anaerobic, Nitrogen-Fixing Bacterium, strain MSSRF40(T), was isolated from roots of mangrove-associated wild rice (Porteresia coarctata Tateoka). On the basis of 16S rRNA gene sequence similarities, strain MSSRF40(T) was shown to belong to the family Enterobacteriaceae, most closely related to Cronobacter muytjensii E603(T) (97.2 % sequence similarity), Enterobacter cloacae subsp. dissolvens LMG 2683(T) (97.1 %), E. radicincitans D5/23(T) (97.1 %) and E. ludwigii EN-119(T) (97.0 %). Sequence analysis of rpoB, gyrB and hsp60 genes showed that strain MSSRF40(T) had relatively low sequence similarity (

Gary P Roberts - One of the best experts on this subject based on the ideXlab platform.

  • glnd is essential for nifa activation ntrb ntrc regulated gene expression and posttranslational regulation of nitrogenase activity in the photosynthetic nitrogen fixing Bacterium rhodospirillum rubrum
    Journal of Bacteriology, 2005
    Co-Authors: Yaoping Zhang, Edward L Pohlmann, Gary P Roberts
    Abstract:

    GlnD is a bifunctional uridylyltransferase/uridylyl-removing enzyme and is thought to be the primary sensor of nitrogen status in the cell. It plays an important role in nitrogen assimilation and metabolism by reversibly regulating the modification of PII proteins, which in turn regulate a variety of other proteins. We report here the characterization of glnD mutants from the photosynthetic, Nitrogen-Fixing Bacterium Rhodospirillum rubrum and the analysis of the roles of GlnD in the regulation of nitrogen fixation. Unlike glnD mutations in Azotobacter vinelandii and some other bacteria, glnD deletion mutations are not lethal in R. rubrum. Such mutants grew well in minimal medium with glutamate as the sole nitrogen source, although they grew slowly with ammonium as the sole nitrogen source (MN medium) and were unable to fix N2. The slow growth in MN medium is apparently due to low glutamine synthetase activity, because a ΔglnD strain with an altered glutamine synthetase that cannot be adenylylated can grow well in MN medium. Various mutation and complementation studies were used to show that the critical uridylyltransferase activity of GlnD is localized to the N-terminal region. Mutants with intermediate levels of uridylyltransferase activity are differentially defective in nif gene expression, the posttranslational regulation of nitrogenase, and NtrB/NtrC function, indicating the complexity of the physiological role of GlnD. These results have implications for the interpretation of results obtained with GlnD in many other organisms.

  • GlnD Is Essential for NifA Activation, NtrB/NtrC-Regulated Gene Expression, and Posttranslational Regulation of Nitrogenase Activity in the Photosynthetic, Nitrogen-Fixing Bacterium Rhodospirillum rubrum
    Journal of Bacteriology, 2005
    Co-Authors: Yaoping Zhang, Edward L Pohlmann, Gary P Roberts
    Abstract:

    GlnD is a bifunctional uridylyltransferase/uridylyl-removing enzyme and is thought to be the primary sensor of nitrogen status in the cell. It plays an important role in nitrogen assimilation and metabolism by reversibly regulating the modification of PII proteins, which in turn regulate a variety of other proteins. We report here the characterization of glnD mutants from the photosynthetic, Nitrogen-Fixing Bacterium Rhodospirillum rubrum and the analysis of the roles of GlnD in the regulation of nitrogen fixation. Unlike glnD mutations in Azotobacter vinelandii and some other bacteria, glnD deletion mutations are not lethal in R. rubrum. Such mutants grew well in minimal medium with glutamate as the sole nitrogen source, although they grew slowly with ammonium as the sole nitrogen source (MN medium) and were unable to fix N2. The slow growth in MN medium is apparently due to low glutamine synthetase activity, because a ΔglnD strain with an altered glutamine synthetase that cannot be adenylylated can grow well in MN medium. Various mutation and complementation studies were used to show that the critical uridylyltransferase activity of GlnD is localized to the N-terminal region. Mutants with intermediate levels of uridylyltransferase activity are differentially defective in nif gene expression, the posttranslational regulation of nitrogenase, and NtrB/NtrC function, indicating the complexity of the physiological role of GlnD. These results have implications for the interpretation of results obtained with GlnD in many other organisms.

Sergio Miana Faria - One of the best experts on this subject based on the ideXlab platform.

  • Mycorrhizal networks facilitate the colonization of legume roots by a symbiotic Nitrogen-Fixing Bacterium
    Mycorrhiza, 2020
    Co-Authors: Candido Barreto Novais, Cristiana Sbrana, Ederson Conceição Jesus, Luc Felicianus Marie Rouws, Manuela Giovannetti, Luciano Avio, José Oswaldo Siqueira, Orivaldo José Saggin Júnior, Eliane Maria Ribeiro Silva, Sergio Miana Faria
    Abstract:

    Arbuscular mycorrhizal fungi (AMF) absorb and translocate nutrients from soil to their host plants by means of a wide network of extraradical mycelium (ERM). Here, we assessed whether Nitrogen-Fixing rhizobia can be transferred to the host legume Glycine max by ERM produced by Glomus formosanum isolate CNPAB020 colonizing the grass Urochloa decumbens . An H-bridge experimental system was developed to evaluate the migration of ERM and of the GFP-tagged Bradyrhizobium diazoefficiens USDA 110 strain across an air gap compartment. Mycorrhizal colonization, nodule formation in legumes, and occurrence of the GFP-tagged strain in root nodules were assessed by optical and confocal laser scanning microscopy. In the presence of non-mycorrhizal U. decumbens , legume roots were neither AMF-colonized nor nodulated. In contrast, G. formosanum ERM crossing the discontinuous compartment connected mycorrhizal U. decumbens and G. max roots, which showed 30–42% mycorrhizal colonization and 7–11 nodules per plant. Fluorescent B. diazoefficiens cells were detected in 94% of G. max root nodules. Our findings reveal that, besides its main activity in nutrient transfer, ERM produced by AMF may facilitate bacterial translocation and the simultaneous associations of plants with beneficial fungi and bacteria, representing an important structure, functional to the establishment of symbiotic relationships.