The Experts below are selected from a list of 49332 Experts worldwide ranked by ideXlab platform
Giovanni Ponti - One of the best experts on this subject based on the ideXlab platform.
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multiphoton laser tomography and fluorescence lifetime imaging of melanoma morphologic features and quantitative data for sensitive and specific non invasive Diagnostics
PLOS ONE, 2013Co-Authors: Stefania Seidenari, Federica Arginelli, Christopher Dunsby, P M W French, Karsten Konig, Cristina Magnoni, Clifford Talbot, Giovanni PontiAbstract:Multiphoton laser tomography (MPT) combined with fluorescence lifetime imaging (FLIM) is a Non-Invasive imaging technique, based on the study of fluorescence decay times of naturally occurring fluorescent molecules, enabling a Non-Invasive investigation of the skin with subcellular resolution. The aim of this retrospective observational ex vivo study, was to characterize melanoma both from a morphologic and a quantitative point of view, attaining an improvement in the diagnostic accuracy with respect to dermoscopy. In the training phase, thirty parameters, comprising both cytological descriptors and architectural aspects, were identified. The training set included 6 melanomas with a mean Breslow thickness±S.D. of 0.89±0.48 mm. In the test phase, these parameters were blindly evaluated on a test data set consisting of 25 melanomas, 50 nevi and 50 basal cell carcinomas. Melanomas in the test phase comprised 8 in situ lesions and had a mean thickness±S.D. of 0.77±1.2 mm. Moreover, quantitative FLIM data were calculated for special areas of interest. Melanoma was characterized by the presence of atypical short lifetime cells and architectural disorder, in contrast to nevi presenting typical cells and a regular histoarchitecture. Sensitivity and specificity values for melanoma diagnosis were 100% and 98%, respectively, whereas dermoscopy achieved the same sensitivity, but a lower specificity (82%). Mean fluorescence lifetime values of melanocytic cells did not vary between melanomas and nevi, but significantly differed from those referring to basal cell carcinoma enabling a differential diagnosis based on quantitative data. Data from prospective preoperative trials are needed to confirm if MPT/FLIM could increase diagnostic specificity and thus reduce unnecessary surgical excisions.
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multiphoton laser tomography and fluorescence lifetime imaging of basal cell carcinoma morphologic features for non invasive Diagnostics
Experimental Dermatology, 2012Co-Authors: Stefania Seidenari, Federica Arginelli, Christopher Dunsby, P M W French, Karsten Konig, Cristina Magnoni, Marco Manfredini, Clifford Talbot, Giovanni PontiAbstract:: Multiphoton laser tomography (MPT) combined with fluorescence lifetime imaging (FLIM) is a Non-Invasive imaging technique, which gives access to the cellular and extracellular morphology of the skin. The aim of our study was to assess the sensitivity and specificity of MPT/FLIM descriptors for basal cell carcinoma (BCC), to improve BCC diagnosis and the identification of tumor margins. In the preliminary study, FLIM images referring to 35 BCCs and 35 healthy skin samples were evaluated for the identification of morphologic descriptors characteristic of BCC. In the main study, the selected parameters were blindly evaluated on a test set comprising 63 BCCs, 63 healthy skin samples and 66 skin lesions. Moreover, FLIM values inside a region of interest were calculated on 98 healthy skin and 98 BCC samples. In the preliminary study, three epidermal descriptors and 7 BCC descriptors were identified. The specificity of the diagnostic criteria versus 'other lesions' was extremely high, indicating that the presence of at least one BCC descriptor makes the diagnosis of 'other lesion' extremely unlikely. FLIM values referring to BCC cells significantly differed from those of healthy skin. In this study, we identified morphological and numerical descriptors enabling the differentiation of BCC from other skin disorders and its distinction from healthy skin in ex vivo samples. In future, MPT/FLIM may be applied to skin lesions to provide direct clinical guidance before biopsy and histological examination and for the identification of tumor margins allowing a complete surgical removal.
Giuseppe Cringoli - One of the best experts on this subject based on the ideXlab platform.
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Mini-FLOTAC as an alternative, Non-Invasive diagnostic tool for Schistosoma mansoni and other trematode infections in wildlife reservoirs
Parasites & Vectors, 2019Co-Authors: Stefano Catalano, Amelia Symeou, Kirsty J. Marsh, Anna Borlase, Elsa Léger, Cheikh B. Fall, Mariama Sène, Nicolas D. Diouf, Davide Ianniello, Giuseppe CringoliAbstract:Background Schistosomiasis and food-borne trematodiases are not only of major public health concern, but can also have profound implications for livestock production and wildlife conservation. The zoonotic, multi-host nature of many digenean trematodes is a significant challenge for disease control programmes in endemic areas. However, our understanding of the epidemiological role that animal reservoirs, particularly wild hosts, may play in the transmission of zoonotic trematodiases suffers a dearth of information, with few, if any, standardised, reliable diagnostic tests available. We combined qualitative and quantitative data derived from post-mortem examinations, coprological analyses using the Mini-FLOTAC technique, and molecular tools to assess parasite community composition and the validity of Non-Invasive methods to detect trematode infections in 89 wild Hubert’s multimammate mice ( Mastomys huberti ) from northern Senegal. Results Parasites isolated at post-mortem examination were identified as Plagiorchis sp., Anchitrema sp., Echinostoma caproni , Schistosoma mansoni , and a hybrid between Schistosoma haematobium and Schistosoma bovis . The reports of E. caproni and Anchitrema sp. represent the first molecularly confirmed identifications for these trematodes in definitive hosts of sub-Saharan Africa. Comparison of prevalence estimates derived from parasitological analysis at post-mortem examination and Mini-FLOTAC analysis showed non-significant differences indicating comparable results between the two techniques ( P = 1.00 for S. mansoni ; P = 0.85 for E. caproni ; P = 0.83 for Plagiorchis sp.). A Bayesian model, applied to estimate the sensitivities of the two tests for the diagnosis of Schistosoma infections, indicated similar median posterior probabilities of 83.1% for Mini-FLOTAC technique and 82.9% for post-mortem examination (95% Bayesian credible intervals of 64.0–94.6% and 63.7–94.7%, respectively). Conclusions Our results showed that the Mini-FLOTAC could be applied as an alternative diagnostic technique for the detection of the zoonotic S. mansoni and other trematodes in rodent reservoirs. The implementation of Non-Invasive Diagnostics in wildlife would offer numerous advantages over lethal sampling methodologies, with potential impact on control strategies of zoonotic helminthiases in endemic areas of sub-Saharan Africa and on fostering a framework of animal use reduction in scientific practice.
Stefania Seidenari - One of the best experts on this subject based on the ideXlab platform.
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multiphoton laser tomography and fluorescence lifetime imaging of melanoma morphologic features and quantitative data for sensitive and specific non invasive Diagnostics
PLOS ONE, 2013Co-Authors: Stefania Seidenari, Federica Arginelli, Christopher Dunsby, P M W French, Karsten Konig, Cristina Magnoni, Clifford Talbot, Giovanni PontiAbstract:Multiphoton laser tomography (MPT) combined with fluorescence lifetime imaging (FLIM) is a Non-Invasive imaging technique, based on the study of fluorescence decay times of naturally occurring fluorescent molecules, enabling a Non-Invasive investigation of the skin with subcellular resolution. The aim of this retrospective observational ex vivo study, was to characterize melanoma both from a morphologic and a quantitative point of view, attaining an improvement in the diagnostic accuracy with respect to dermoscopy. In the training phase, thirty parameters, comprising both cytological descriptors and architectural aspects, were identified. The training set included 6 melanomas with a mean Breslow thickness±S.D. of 0.89±0.48 mm. In the test phase, these parameters were blindly evaluated on a test data set consisting of 25 melanomas, 50 nevi and 50 basal cell carcinomas. Melanomas in the test phase comprised 8 in situ lesions and had a mean thickness±S.D. of 0.77±1.2 mm. Moreover, quantitative FLIM data were calculated for special areas of interest. Melanoma was characterized by the presence of atypical short lifetime cells and architectural disorder, in contrast to nevi presenting typical cells and a regular histoarchitecture. Sensitivity and specificity values for melanoma diagnosis were 100% and 98%, respectively, whereas dermoscopy achieved the same sensitivity, but a lower specificity (82%). Mean fluorescence lifetime values of melanocytic cells did not vary between melanomas and nevi, but significantly differed from those referring to basal cell carcinoma enabling a differential diagnosis based on quantitative data. Data from prospective preoperative trials are needed to confirm if MPT/FLIM could increase diagnostic specificity and thus reduce unnecessary surgical excisions.
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multiphoton laser tomography and fluorescence lifetime imaging of basal cell carcinoma morphologic features for non invasive Diagnostics
Experimental Dermatology, 2012Co-Authors: Stefania Seidenari, Federica Arginelli, Christopher Dunsby, P M W French, Karsten Konig, Cristina Magnoni, Marco Manfredini, Clifford Talbot, Giovanni PontiAbstract:: Multiphoton laser tomography (MPT) combined with fluorescence lifetime imaging (FLIM) is a Non-Invasive imaging technique, which gives access to the cellular and extracellular morphology of the skin. The aim of our study was to assess the sensitivity and specificity of MPT/FLIM descriptors for basal cell carcinoma (BCC), to improve BCC diagnosis and the identification of tumor margins. In the preliminary study, FLIM images referring to 35 BCCs and 35 healthy skin samples were evaluated for the identification of morphologic descriptors characteristic of BCC. In the main study, the selected parameters were blindly evaluated on a test set comprising 63 BCCs, 63 healthy skin samples and 66 skin lesions. Moreover, FLIM values inside a region of interest were calculated on 98 healthy skin and 98 BCC samples. In the preliminary study, three epidermal descriptors and 7 BCC descriptors were identified. The specificity of the diagnostic criteria versus 'other lesions' was extremely high, indicating that the presence of at least one BCC descriptor makes the diagnosis of 'other lesion' extremely unlikely. FLIM values referring to BCC cells significantly differed from those of healthy skin. In this study, we identified morphological and numerical descriptors enabling the differentiation of BCC from other skin disorders and its distinction from healthy skin in ex vivo samples. In future, MPT/FLIM may be applied to skin lesions to provide direct clinical guidance before biopsy and histological examination and for the identification of tumor margins allowing a complete surgical removal.
Sergey Piletsky - One of the best experts on this subject based on the ideXlab platform.
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Vanishing tattoo multi-sensor for biomedical Diagnostics
Biophotonics: Photonic Solutions for Better Health Care, 2008Co-Authors: E. Moczko, Igor Meglinski, Sergey PiletskyAbstract:Currently, precise Non-Invasive Diagnostics systems for the real-time multi detection and monitoring of physiological parameters and chemical analytes in the human body are urgently required by clinicians, physiologists and bio-medical researchers. We have developed a novel cost effective smart 'vanishing tattoo' (similar to temporary child's tattoos) consisting of environmental-sensitive dyes. Painlessly impregnated into the skin the smart tattoo is capable of generating optical/fluorescence changes (absorbance, transmission, reflectance, emission and/or luminescence within UV, VIS or NIR regions) in response to physical or chemical changes. These changes allow the identification of colour pattern changes similar to bar-code scanning. Such a system allows an easy, cheap and robust comprehensive detection of various parameters and analytes in a small volume of sample (e.g. variations in pH, temperature, ionic strength, solvent polarity, presence of redox species, surfactants, oxygen). These smart tattoos have possible applications in monitoring the progress of disease and transcutaneous drug delivery. The potential of this highly innovative diagnostic tool is wide and diverse and can impact on routine clinical Diagnostics, general therapeutic management, skin care and cosmetic products testing as well as fundamental physiological investigations.
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Vanishing "tattoo" multisensor for biomedical Diagnostics
Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2008Co-Authors: E. Moczko, Igor Meglinski, Sergey PiletskyAbstract:Currently, precise Non-Invasive Diagnostics systems for the real-time multi detection and monitoring of physiological parameters and chemical analytes in the human body are urgently required by clinicians, physiologists and bio-medical researchers. We have developed a novel cost effective smart 'vanishing tattoo' (similar to temporary child's tattoos) consisting of environmental-sensitive dyes. Painlessly impregnated into the skin the smart tattoo is capable of generating optical/fluorescence changes (absorbance, transmission, reflectance, emission and/or luminescence within UV, VIS or NIR regions) in response to physical or chemical changes. These changes allow the identification of colour pattern changes similar to bar-code scanning. Such a system allows an easy, cheap and robust comprehensive detection of various parameters and analytes in a small volume of sample (e.g. variations in pH, temperature, ionic strength, solvent polarity, presence of redox species, surfactants, oxygen). These smart tattoos have possible applications in monitoring the progress of disease and transcutaneous drug delivery. The potential of this highly innovative diagnostic tool is wide and diverse and can impact on routine clinical Diagnostics, general therapeutic management, skin care and cosmetic products testing as well as fundamental physiological investigations.
Marica B Ericson - One of the best experts on this subject based on the ideXlab platform.
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multiphoton laser scanning microscopy on non melanoma skin cancer morphologic features for future non invasive Diagnostics
Journal of Investigative Dermatology, 2008Co-Authors: John Paoli, Maria Smedh, Annmarie Wennberg, Marica B EricsonAbstract:This study describes the morphologic features of human non-melanoma skin cancer obtained using multiphoton laser scanning microscopy (MPLSM) on freshly excised specimens from 14 patients. Optical sectioning parallel to the tissue surface was performed, resulting in en face autofluorescence images of the epidermis and upper dermis, reaching tissue depths of 135mm. The microscopy was carried out ex vivo using a femtosecond pulsed laser at 780nm and a 40/0.8 objective. The autofluorescence was detected in the range of 450–530nm. Traditional histopathological criteria such as bowenoid dysplasia, multinucleated cells, or hyperkeratosis in squamous cell carcinoma in situ (SCCIS) (five specimens), and peripheral palisading of tumor cells in superficial basal cell carcinoma (SBCC) (six specimens) were clearly discerned. The morphologic features differed significantly between these lesions and perilesional skin. However, characteristic tumor aggregates were found in only one of the three investigated nodular basal cell carcinomas (NBCCs) due to limited imaging depth. In addition, speckled perinuclear fluorescence was observed in both lesions and normal perilesional skin. In conclusion, MPLSM could potentially be applied for Non-Invasive Diagnostics of SCCIS and SBCC, whereas the ability to characterize NBCC is unclear at this point.