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Wafaa Alalawi - One of the best experts on this subject based on the ideXlab platform.

  • antibiotic resistant bacteria as bio indicator of polluted effluent in the green turtles chelonia mydas in oman
    Marine Environmental Research, 2011
    Co-Authors: Saif N Albahry, I Y Mahmoud, Maheera Alzadjali, A E Elshafie, Asila Alharthy, Wafaa Alalawi
    Abstract:

    Antibiotic resistant bacteria were studied as bio-indicators of marine polluted effluents during egg-laying in green turtles. A Non-Invasive Procedure for sampling oviductal fluid was used to test for exposure of turtles to pollution in Ras Al-Hadd, Oman, which is one of the most important nesting beaches in the world. Each sample was obtained by inserting a 15 cm sterile swab gently into the cloacal vent as the sphincter muscle is relaxed and the cloacal lining is unfolded to the outside. Forty turtles were sampled. A hundred and thirty-two species of bacteria from 7 genera were isolated. The dominant isolate was Citrobacter. Among the isolates 60.6% were multiple resistant to 15 tested antibiotics. The dominant resistance to antibiotics was ampicillin followed by streptomycin and sulphamethoxazole. Sampling oviductal fluid for resistant bacteria to antibiotics is valuable way to assess exposure to polluted effluents during feeding and migratory in turtles. Polluted effluents using bacteria as bio-indicator may influence reproductive potential in this endangered species.

  • antibiotic resistant bacteria as bio indicator of polluted effluent in the green turtles chelonia mydas in oman
    Marine Environmental Research, 2011
    Co-Authors: Saif N Albahry, I Y Mahmoud, Maheera Alzadjali, A E Elshafie, Asila Alharthy, Wafaa Alalawi
    Abstract:

    Abstract Antibiotic resistant bacteria were studied as bio-indicators of marine polluted effluents during egg-laying in green turtles. A Non-Invasive Procedure for sampling oviductal fluid was used to test for exposure of turtles to pollution in Ras Al-Hadd, Oman, which is one of the most important nesting beaches in the world. Each sample was obtained by inserting a 15 cm sterile swab gently into the cloacal vent as the sphincter muscle is relaxed and the cloacal lining is unfolded to the outside. Forty turtles were sampled. A hundred and thirty-two species of bacteria from 7 genera were isolated. The dominant isolate was Citrobacter . Among the isolates 60.6% were multiple resistant to 15 tested antibiotics. The dominant resistance to antibiotics was ampicillin followed by streptomycine and sulphamethoxazole. Sampling oviductal fluid for resistant bacteria to antibiotics is valuable way to assess exposure to polluted effluents during feeding and migratory in turtles. Polluted effluents using bacteria as bio-indicator may influence reproductive potential in this endangered species.

Barry J Pogson - One of the best experts on this subject based on the ideXlab platform.

  • a rapid Non Invasive Procedure for quantitative assessment of drought survival using chlorophyll fluorescence
    Plant Methods, 2008
    Co-Authors: Murray R Badger, Barry J Pogson
    Abstract:

    Background Analysis of survival is commonly used as a means of comparing the performance of plant lines under drought. However, the assessment of plant water status during such studies typically involves detachment to estimate water shock, imprecise methods of estimation or Invasive measurements such as osmotic adjustment that influence or annul further evaluation of a specimen's response to drought.

  • a rapid Non Invasive Procedure for quantitative assessment of drought survival using chlorophyll fluorescence
    Plant Methods, 2008
    Co-Authors: Nick S Woo, Murray R Badger, Barry J Pogson
    Abstract:

    Analysis of survival is commonly used as a means of comparing the performance of plant lines under drought. However, the assessment of plant water status during such studies typically involves detachment to estimate water shock, imprecise methods of estimation or Invasive measurements such as osmotic adjustment that influence or annul further evaluation of a specimen's response to drought. This article presents a Procedure for rapid, inexpensive and Non-Invasive assessment of the survival of soil-grown plants during drought treatment. The changes in major photosynthetic parameters during increasing water deficit were monitored via chlorophyll fluorescence imaging and the selection of the maximum efficiency of photosystem II (Fv/Fm) parameter as the most straightforward and practical means of monitoring survival is described. The veracity of this technique is validated through application to a variety of Arabidopsis thaliana ecotypes and mutant lines with altered tolerance to drought or reduced photosynthetic efficiencies. The method presented here allows the acquisition of quantitative numerical estimates of Arabidopsis drought survival times that are amenable to statistical analysis. Furthermore, the required measurements can be obtained quickly and Non-Invasively using inexpensive equipment and with minimal expertise in chlorophyll fluorometry. This technique enables the rapid assessment and comparison of the relative viability of germplasm during drought, and may complement detailed physiological and water relations studies.

A E Elshafie - One of the best experts on this subject based on the ideXlab platform.

  • Biomonitoring marine habitats in reference to antibiotic resistant bacteria and ampicillin resistance determinants from oviductal fluid of the nesting green sea turtle, Chelonia mydas.
    Chemosphere, 2012
    Co-Authors: Saif N. Al-bahry, M.a. Al-zadjali, Ibrahim Y. Mahmoud, A E Elshafie
    Abstract:

    During the egg-laying process, oviductal fluid was collected using a Non-Invasive Procedure from the cloacal vent of the green turtles. Forty-two independent isolates of antibiotic-resistant bacteria from 11 genera were obtained from 20 turtles during nesting. The dominant isolate was Citrobacter (52.4%), followed by Pseudomonas, Proteus, Enterobacter, Salmonella, Escherichia coli, Shigella, Edwardsiella, Morganella, Providencia and Arcomobacter. Most of the isolates were resistant to ampicillin. Ampicillin-resistant isolates showed variations in their resistance for the following classes of β-lactamases: extended-spectrum β-lactamases (EBSLs), AmpC type β-lactamases C (AmpC), and screen-positive β-lactamase. None of the isolates produced metallo β-lactamase. Some ampicillin-resistant genes were detected by multiplex polymerase chain reaction (PCR) only. Inhibitor based test (IBT) categorized some isolates as AmpC β-lactamase producers. β-Lactamase genes were detected from a few strains. The sequencing of those genes revealed the presence of cephamycinase (CMY) and AmpC β-lactamases. The oviductal fluid was used in this study as a source of bacterial antibiotic-resistant determinants for biomonitoring marine turtles exposed to contaminated effluents. This data can be of value in understanding the decline of this endangered species as a result of exposure to marine pollution which is threatening their survival.

  • antibiotic resistant bacteria as bio indicator of polluted effluent in the green turtles chelonia mydas in oman
    Marine Environmental Research, 2011
    Co-Authors: Saif N Albahry, I Y Mahmoud, Maheera Alzadjali, A E Elshafie, Asila Alharthy, Wafaa Alalawi
    Abstract:

    Antibiotic resistant bacteria were studied as bio-indicators of marine polluted effluents during egg-laying in green turtles. A Non-Invasive Procedure for sampling oviductal fluid was used to test for exposure of turtles to pollution in Ras Al-Hadd, Oman, which is one of the most important nesting beaches in the world. Each sample was obtained by inserting a 15 cm sterile swab gently into the cloacal vent as the sphincter muscle is relaxed and the cloacal lining is unfolded to the outside. Forty turtles were sampled. A hundred and thirty-two species of bacteria from 7 genera were isolated. The dominant isolate was Citrobacter. Among the isolates 60.6% were multiple resistant to 15 tested antibiotics. The dominant resistance to antibiotics was ampicillin followed by streptomycin and sulphamethoxazole. Sampling oviductal fluid for resistant bacteria to antibiotics is valuable way to assess exposure to polluted effluents during feeding and migratory in turtles. Polluted effluents using bacteria as bio-indicator may influence reproductive potential in this endangered species.

  • antibiotic resistant bacteria as bio indicator of polluted effluent in the green turtles chelonia mydas in oman
    Marine Environmental Research, 2011
    Co-Authors: Saif N Albahry, I Y Mahmoud, Maheera Alzadjali, A E Elshafie, Asila Alharthy, Wafaa Alalawi
    Abstract:

    Abstract Antibiotic resistant bacteria were studied as bio-indicators of marine polluted effluents during egg-laying in green turtles. A Non-Invasive Procedure for sampling oviductal fluid was used to test for exposure of turtles to pollution in Ras Al-Hadd, Oman, which is one of the most important nesting beaches in the world. Each sample was obtained by inserting a 15 cm sterile swab gently into the cloacal vent as the sphincter muscle is relaxed and the cloacal lining is unfolded to the outside. Forty turtles were sampled. A hundred and thirty-two species of bacteria from 7 genera were isolated. The dominant isolate was Citrobacter . Among the isolates 60.6% were multiple resistant to 15 tested antibiotics. The dominant resistance to antibiotics was ampicillin followed by streptomycine and sulphamethoxazole. Sampling oviductal fluid for resistant bacteria to antibiotics is valuable way to assess exposure to polluted effluents during feeding and migratory in turtles. Polluted effluents using bacteria as bio-indicator may influence reproductive potential in this endangered species.

Saif N Albahry - One of the best experts on this subject based on the ideXlab platform.

  • antibiotic resistant bacteria as bio indicator of polluted effluent in the green turtles chelonia mydas in oman
    Marine Environmental Research, 2011
    Co-Authors: Saif N Albahry, I Y Mahmoud, Maheera Alzadjali, A E Elshafie, Asila Alharthy, Wafaa Alalawi
    Abstract:

    Antibiotic resistant bacteria were studied as bio-indicators of marine polluted effluents during egg-laying in green turtles. A Non-Invasive Procedure for sampling oviductal fluid was used to test for exposure of turtles to pollution in Ras Al-Hadd, Oman, which is one of the most important nesting beaches in the world. Each sample was obtained by inserting a 15 cm sterile swab gently into the cloacal vent as the sphincter muscle is relaxed and the cloacal lining is unfolded to the outside. Forty turtles were sampled. A hundred and thirty-two species of bacteria from 7 genera were isolated. The dominant isolate was Citrobacter. Among the isolates 60.6% were multiple resistant to 15 tested antibiotics. The dominant resistance to antibiotics was ampicillin followed by streptomycin and sulphamethoxazole. Sampling oviductal fluid for resistant bacteria to antibiotics is valuable way to assess exposure to polluted effluents during feeding and migratory in turtles. Polluted effluents using bacteria as bio-indicator may influence reproductive potential in this endangered species.

  • antibiotic resistant bacteria as bio indicator of polluted effluent in the green turtles chelonia mydas in oman
    Marine Environmental Research, 2011
    Co-Authors: Saif N Albahry, I Y Mahmoud, Maheera Alzadjali, A E Elshafie, Asila Alharthy, Wafaa Alalawi
    Abstract:

    Abstract Antibiotic resistant bacteria were studied as bio-indicators of marine polluted effluents during egg-laying in green turtles. A Non-Invasive Procedure for sampling oviductal fluid was used to test for exposure of turtles to pollution in Ras Al-Hadd, Oman, which is one of the most important nesting beaches in the world. Each sample was obtained by inserting a 15 cm sterile swab gently into the cloacal vent as the sphincter muscle is relaxed and the cloacal lining is unfolded to the outside. Forty turtles were sampled. A hundred and thirty-two species of bacteria from 7 genera were isolated. The dominant isolate was Citrobacter . Among the isolates 60.6% were multiple resistant to 15 tested antibiotics. The dominant resistance to antibiotics was ampicillin followed by streptomycine and sulphamethoxazole. Sampling oviductal fluid for resistant bacteria to antibiotics is valuable way to assess exposure to polluted effluents during feeding and migratory in turtles. Polluted effluents using bacteria as bio-indicator may influence reproductive potential in this endangered species.

Adrien Dubois - One of the best experts on this subject based on the ideXlab platform.

  • towards translational immunopet mr imaging of Invasive pulmonary aspergillosis the humanised monoclonal antibody jf5 detects aspergillus lung infections in vivo
    Theranostics, 2017
    Co-Authors: Genna Davies, Annamaria Rolle, Andreas Maurer, Philipp R Spycher, Claudia Schillinger, Djamschid Solouksaran, Mike Hasenberg, Juliane Weski, Jesper Fonslet, Adrien Dubois
    Abstract:

    Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease of hematological malignancy or bone marrow transplant patients caused by the ubiquitous environmental fungus Aspergillus fumigatus. Current diagnostic tests for the disease lack sensitivity as well as specificity, and culture of the fungus from Invasive lung biopsy, considered the gold standard for IPA detection, is slow and often not possible in critically ill patients. In a previous study, we reported the development of a novel Non-Invasive Procedure for IPA diagnosis based on antibody-guided positron emission tomography and magnetic resonance imaging (immunoPET/MRI) using a [64Cu]DOTA-labeled mouse monoclonal antibody (mAb), mJF5, specific to Aspergillus. To enable translation of the tracer to the clinical setting, we report here the development of a humanised version of the antibody (hJF5), and pre-clinical imaging of lung infection using a [64Cu]NODAGA-hJF5 tracer. The humanised antibody tracer shows a significant increase in in vivo biodistribution in A. fumigatus infected lungs compared to its radiolabeled murine counterpart [64Cu]NODAGA-mJF5. Using reverse genetics of the pathogen, we show that the antibody binds to the antigenic determinant β1,5-galactofuranose (Galf) present in a diagnostic mannoprotein antigen released by the pathogen during Invasive growth in the lung. The absence of the epitope Galf in mammalian carbohydrates, coupled with the enhanced imaging capabilities of the hJF5 antibody, means that the [64Cu]NODAGA-hJF5 tracer developed here represents an ideal candidate for the diagnosis of IPA and translation to the clinical setting.