The Experts below are selected from a list of 138 Experts worldwide ranked by ideXlab platform
M Vijayan - One of the best experts on this subject based on the ideXlab platform.
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effect of linkage on the location of reducing and Nonreducing Sugars bound to jacalin
Iubmb Life, 2016Co-Authors: K V Abhinav, K Sharma, Avadhesha Surolia, M VijayanAbstract:The crystal structures of jacalin complexed with Gal alpha-(1,4) Gal and Gal alpha-(1,3) Gal beta-(1,4) Gal have been determined with the primary objective of exploring the effect of linkage on the location of reducing and non-reducing Sugars in the extended binding site of the lectin, an issue which has not been studied thoroughly. Contrary to the earlier surmise based on simple steric considerations, the two structures demonstrate that alpha-linked Sugars can bind to jacalin with Nonreducing sugar at the primary binding site. This is made possible substantially on account of the hitherto underestimated plasticity of a non-polar region of the extended binding site. Modeling studies involving conformational search and energy minimization, along with available crystallographic and thermodynamic data, indicate a strong preference for complexation with Gal beta-(1,3) Gal with the reducing Gal at the primary site, followed by that with Gal alpha-(1,3) Gal, with the reducing or non-reducing Gal located at the primary binding site. This observation is in consonance with the facility of jacalin to bind mucin type O-glycans containing T-antigen core. (C) 2016 IUBMB Life, 68(12): 971-979, 2016
K V Abhinav - One of the best experts on this subject based on the ideXlab platform.
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effect of linkage on the location of reducing and Nonreducing Sugars bound to jacalin
Iubmb Life, 2016Co-Authors: K V Abhinav, K Sharma, Avadhesha Surolia, M VijayanAbstract:The crystal structures of jacalin complexed with Gal alpha-(1,4) Gal and Gal alpha-(1,3) Gal beta-(1,4) Gal have been determined with the primary objective of exploring the effect of linkage on the location of reducing and non-reducing Sugars in the extended binding site of the lectin, an issue which has not been studied thoroughly. Contrary to the earlier surmise based on simple steric considerations, the two structures demonstrate that alpha-linked Sugars can bind to jacalin with Nonreducing sugar at the primary binding site. This is made possible substantially on account of the hitherto underestimated plasticity of a non-polar region of the extended binding site. Modeling studies involving conformational search and energy minimization, along with available crystallographic and thermodynamic data, indicate a strong preference for complexation with Gal beta-(1,3) Gal with the reducing Gal at the primary site, followed by that with Gal alpha-(1,3) Gal, with the reducing or non-reducing Gal located at the primary binding site. This observation is in consonance with the facility of jacalin to bind mucin type O-glycans containing T-antigen core. (C) 2016 IUBMB Life, 68(12): 971-979, 2016
Bala Nambisan - One of the best experts on this subject based on the ideXlab platform.
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Carbohydrate Metabolism during Dormancy and Sprouting in Yam (Dioscorea) Tubers: Changes in Carbohydrate Constituents in Yam (Dioscorea) Tubers during Dormancy and Sprouting
Journal of Agricultural and Food Chemistry, 1996Co-Authors: C. S. Hariprakash, Bala NambisanAbstract:Changes in carbohydrate constituents were stuided in tubers of Dioscorea rotundata, Dioscorea alata, and Dioscorea esculenta during dormancy and sprouting for a period of 90 days. Decrease in starch content (35%) was the most significant change observed in all yam species. This decrease was reflected in proximal, middle, and distal regions of tubers. More than 50% of the starch reduction took place during the dormancy period. The mobilization of starch to Sugars and its utilization was reflected in fluctuating levels of reducing and Nonreducing Sugars during presprouting/sprouting periods. Changes were also observed in the amylose fraction in starch. Chromatographic sugar analysis in tubers indicated the presence of glucose and sucrose in fresh tubers. Fructose and maltose were detected during dormancy/sprouting periods. Keywords: Dioscorea tubers; carbohydrates; dormancy; sprouting
Paul Thomas - One of the best experts on this subject based on the ideXlab platform.
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Effects of gamma irradiation and temperature on sugar and vitamin C changes in five Indian potato cultivars during storage
Food Chemistry, 1990Co-Authors: M. R. Joshi, A. N. Srirangarajan, Paul ThomasAbstract:Abstract The changes in sugar and vitamin C content of five potato cultivars subjected to gamma irradiation (100 Gy) to cause sprout inhibition were compared with nonirradiated control tubers during storage at 27–32°C (ambient temperature) and 15°C, and also with control tubers stored at 2–4°C—the commercial cold storage temperature. Reducing and Nonreducing Sugars increased during extended storage at all temperatures, the maximum increase occurring at 2–4°C. The proportion of Nonreducing Sugars was higher at 27–32°C, whereas at 2–4°C reducing Sugars formed the greater proportion. Irradiation did not influence the pattern of sugar accumulation. Irradiated potatoes stored at 15°C for 6 months had lower sugar levels than control tubers stored at 2–4°C. During the first 2 months vitamin C levels were lower in irradiated potatoes stored at 15°C than in the controls stored at 2–4°C, but were subsequently higher. The suitability of tubers for processing into crisps and french fries was not affected by irradiation and storage at 15°C for 6 months.
K Sharma - One of the best experts on this subject based on the ideXlab platform.
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effect of linkage on the location of reducing and Nonreducing Sugars bound to jacalin
Iubmb Life, 2016Co-Authors: K V Abhinav, K Sharma, Avadhesha Surolia, M VijayanAbstract:The crystal structures of jacalin complexed with Gal alpha-(1,4) Gal and Gal alpha-(1,3) Gal beta-(1,4) Gal have been determined with the primary objective of exploring the effect of linkage on the location of reducing and non-reducing Sugars in the extended binding site of the lectin, an issue which has not been studied thoroughly. Contrary to the earlier surmise based on simple steric considerations, the two structures demonstrate that alpha-linked Sugars can bind to jacalin with Nonreducing sugar at the primary binding site. This is made possible substantially on account of the hitherto underestimated plasticity of a non-polar region of the extended binding site. Modeling studies involving conformational search and energy minimization, along with available crystallographic and thermodynamic data, indicate a strong preference for complexation with Gal beta-(1,3) Gal with the reducing Gal at the primary site, followed by that with Gal alpha-(1,3) Gal, with the reducing or non-reducing Gal located at the primary binding site. This observation is in consonance with the facility of jacalin to bind mucin type O-glycans containing T-antigen core. (C) 2016 IUBMB Life, 68(12): 971-979, 2016
