Nosema locustae

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Carlos E Lange - One of the best experts on this subject based on the ideXlab platform.

  • susceptibilidad de la langosta schistocerca cancellata orthoptera acrididae a Nosema locustae protozoa microspora en laboratorio
    2015
    Co-Authors: Carlos E Lange, Elizabeth Wittenstein, Norma Elba Sanchez
    Abstract:

    El presente estudio tuvo como objetivo determinar la susceptibilidad de Schistocerca cancellata (Serville) al patogeno Nosema locustae Canning, a fin de evaluar posibles alternativas de su control biologico. Ninfas de tercer estadio de la fase gregaria de S. cancellata de bioterio fueron individualmente inoculadas “per os” con 105 y 106 esporos/ninfa de N. locustae, un agente microbiano para el control de acridios. El 90,2 % de las ninfas inoculadas (n = 112) desarrollo infeccion, no existiendo diferencias significativas en el porcentaje de ninfas infectadas entre ambas dosis ensayadas. La mortalidad de las ninfas no fue independiente de la dosis del patogeno, tanto a los 20 como a los 30 dias postinoculacion. Ambas dosis produjeron porcentajes de mortalidad significativamente mas altos que el control. A su vez, la mortalidad acumulada al finalizar la experiencia fue significativamente mas alta con la dosis mayor (86%) que con la dosis menor (50%). Ninguna de las langostas infectadas llego al estado adulto. De ser corroborados estos resultados en condiciones naturales, N. locustae podria ser de utilidad para el control biologico de S. cancellata.

  • the host and geographical range of the grasshopper pathogen paraNosema Nosema locustae revisited
    2005
    Co-Authors: Carlos E Lange
    Abstract:

    Abstract Host and geographical ranges are updated for the microsporidium ParaNosema locustae; this pathogen was developed in the USA as a long-term microbial control agent of grasshoppers. Currently known to be susceptible to P. locustae, either naturally or experimentally, are 121 species of Orthoptera from North and South America, Africa, Australia, China, and India. Most belong to the Acrididae (112), and within this family, to the Melanoplinae (36), Oedipodinae (35), and Gomphocerinae (35). The host range of P. locustae, as presently understood, is based largely on morphology and could change if molecular techniques revealed cryptic species. The North American isolate is not only the best studied, but the one established after its introduction into Argentina, and produced and used in China: it can be considered a generalist pathogen. As such, P. locustae may have the ability to alter, through differences in host susceptibilities, the structure of grasshopper assemblages in areas where it was not prese...

  • niveles de esporulacion experimentales y naturales de Nosema locustae microsporidia en especies de tucuras y langostas orthoptera acridoidea de la argentina
    2003
    Co-Authors: Carlos E Lange
    Abstract:

    Nosema locustae  Canning, an intracellular sporogenic pathogen of the fat body of orthopterans used for the biological control of grasshoppers, became established in some grasshopper communities of Argentina after its introduction more than 20 years ago. Following standard methods for counting spores per individual insect affected, the levels of spore production by  N. locustae  was measured for three species of grasshoppers naturally infected [  Dichroplus elongatus  Giglio-Tos,  Dichroplus pratensis  Bruner,  Baeacris punctulatus  (Thunberg)], and for six species experimentally infected [  Dichroplus maculipennis  Blanchard,  Dichroplus schulzi  Bruner,  D. elongatus, B. punctulatus, Ronderosia bergi  Stal,  Schistocerca cancellata  (Serville)]. The levels of sporulation were relatively high in all species of the subfamily Melanoplinae (mean values varied from 2 x 10 8  to 3,2 x 10 9 ), while the production of spores was rare (9,5 % of the infected individuals) and low (maximum of 9,6 x 10 6 ) in  S. cancellata.  There was no infection development in the Romaleidae grasshopper  Elaeochlora viridicata  (Serville). The values of sporulation reached by the melanoplines agree with the establishment of  N. locustae  in communities of grasshoppers in Argentina because a high availability of infective transmissible units (propagules) is a central factor for the transmission and persistence of a disease in a population of susceptible insects. Although the highest sporulation levels were reached in  D. maculipennis, R. bergi  appears to be, among the species used in this study, the best choice for  in vivo  production of  N. locustae,  because there is no obligatory embrionic diapause in its cycle, and its less reactive behaviour makes the handling of colony easier

  • long term patterns of occurrence of Nosema locustae and perezia dichroplusae microsporidia in grasshoppers orthoptera acrididae of the pampas argentina
    2003
    Co-Authors: Carlos E Lange
    Abstract:

    Summary. The long-term (through the years) occurrence of the two known microsporidia parasitizing grasshoppers of the subfamily Melanoplinae in the Pampas of Argentina (vertically transmitted, native Perezia dichroplusae and horizontally transmitted, introduced Nosema locustae) was monitored at 13 localities from 1995 to 2003. Nosema locustae occurred at nine localities, and P. dichroplusae at five. There was almost no overlap of occurrence, and mixed infections were not registered. The presence and prevalences (1% to 6.1%) of P. dichroplusae were relatively stable over the years in all localities while N. locustae alternated years of presence and absence, and prevalences fluctuated markedly from 1.8% up to 41%. On four instances, each at a different locality, unusually high prevalences (epizootics) of N. locustae were recorded. Natural epizootics of such magnitudes are not known for N. locustae in other regions of the world.

  • Niveles de esporulación experimentales y naturales de Nosema locustae (Microsporidia) en especies de tucuras y langostas (Orthoptera: Acridoidea) de la Argentina Levels of experimental and natural sporulation of Nosema locustae (Microsporidia) in grasshopper and locust species (Orthoptera: Acridoidea) of Argentina
    2003
    Co-Authors: Carlos E Lange
    Abstract:

    Nosema locustae Canning, un patógeno intracelular esporogénico del cuerpo graso de ortópteros utilizado para el control biológico de acridios, se estableció en ciertas comunidades de éstos de la Argentina luego de ser introducido hace poco más de 20 años. En el presente estudio se midieron, siguiendo metodología estandarizada para la estimación del número de esporos por insecto individual afectado, los niveles de esporulación alcanzados por N. locustae en tres especies de acridios naturalmente infectados [ Dichroplus elongatus Giglio-Tos, Dichroplus pratensis Bruner, Baeacris punctulatus (Thunberg)] y en seis especies infectadas experimentalmente [ Dichroplus maculipennis Blanchard, Dichroplus schulzi Bruner, D. elongatus, B. punctulatus, Ronderosia bergi Stal, Schistocerca cancellata (Serville)]. Los niveles de esporulación fueron importantes en todas las especies de la subfamilia Melanoplinae (el promedio varió entre 2 x 10(8) y 3,2 x 10(9)), mientras que la producción de esporos fue rara (9,5 % de los ejemplares infectados) y baja (máxima de 9,6 x 10(6)) en S. cancellata. No ocurrió desarrollo de infección en el acridio Romaleidae Elaeochlora viridicata (Serville). Los valores de esporulación alcanzados en los melanoplinos concuerdan con el establecimiento de N. locustae en comunidades de acridios de la Argentina, pues una alta disponibilidad de unidades infectivas transmisibles (propágulos) es un factor central para la transmisión y persistencia de una enfermedad en una población de insectos susceptibles. Aunque los niveles de esporulación más elevados se alcanzaron en D. maculipennis, R. bergi parece, por la falta de diapausa embrionaria obligatoria en su ciclo y por su comportamiento poco reactivo que facilita el manejo de la colonia, la especie más adecuada de las probadas en este estudio para la producción in vivo de N. locustae.Nosema locustae Canning, an intracellular sporogenic pathogen of the fat body of orthopterans used for the biological control of grasshoppers, became established in some grasshopper communities of Argentina after its introduction more than 20 years ago. Following standard methods for counting spores per individual insect affected, the levels of spore production by N. locustae was measured for three species of grasshoppers naturally infected [ Dichroplus elongatus Giglio-Tos, Dichroplus pratensis Bruner, Baeacris punctulatus (Thunberg)], and for six species experimentally infected [ Dichroplus maculipennis Blanchard, Dichroplus schulzi Bruner, D. elongatus, B. punctulatus, Ronderosia bergi Stal, Schistocerca cancellata (Serville)]. The levels of sporulation were relatively high in all species of the subfamily Melanoplinae (mean values varied from 2 x 10(8) to 3,2 x 10(9)), while the production of spores was rare (9,5 % of the infected individuals) and low (maximum of 9,6 x 10(6)) in S. cancellata. There was no infection development in the Romaleidae grasshopper Elaeochlora viridicata (Serville). The values of sporulation reached by the melanoplines agree with the establishment of N. locustae in communities of grasshoppers in Argentina because a high availability of infective transmissible units (propagules) is a central factor for the transmission and persistence of a disease in a population of susceptible insects. Although the highest sporulation levels were reached in D. maculipennis, R. bergi appears to be, among the species used in this study, the best choice for in vivo production of N. locustae, because there is no obligatory embrionic diapause in its cycle, and its less reactive behaviour makes the handling of colony easier

Patrick J Keeling - One of the best experts on this subject based on the ideXlab platform.

  • transfer of Nosema locustae microsporidia to antonospora locustae n comb based on molecular and ultrastructural data
    2004
    Co-Authors: Claudio H Slamovits, Bryony A P Williams, Patrick J Keeling
    Abstract:

    Nosema locustae is a microsporidian parasite of grasshopper pests that is used as a biological control agent, and is one of the emerging model systems for microsporidia. Due largely to its diplokaryotic nuclei, N. locustae has been classified in the genus Nosema, a large genus with members that infect a wide variety of insects. However, some molecular studies have cast doubt on the validity of certain Nosema species, and on the taxonomic position of N. locustae. To clarify the affinities of this important insect parasite we sequenced part of the rRNA operon of N. locustae and conducted a phylogenetic analysis using the complete small subunit rRNA gene. Nosema locustae is only distantly related to the nominotypic N. bombycis, and is instead closely related to Antonospora scoticae, a recently described parasite of bees. We examined the ultrastructure of mature N. locustae spores, and found the spore wall to differ from true Nosema species in having a multi-layered exospore resembling that of Antonospora (one of the distinguishing features of that genus). Based on both molecular and morphological evidence, therefore, we propose transferring N. locustae to the genus Antonospora, as Antonospora locustae n. comb.

  • bacterial catalase in the microsporidian Nosema locustae implications for microsporidian metabolism and genome evolution
    2003
    Co-Authors: Naomi M Fast, Bryony A P Williams, Joyce S Law, Patrick J Keeling
    Abstract:

    Microsporidia constitute a group of extremely specialized intracellular parasites that infect virtually all animals. They are highly derived, reduced fungi that lack several features typical of other eukaryotes, including canonical mitochondria, flagella, and peroxisomes. Consistent with the absence of peroxisomes in microsporidia, the recently completed genome of the microsporidian Encephalitozoon cuniculi lacks a gene for catalase, the major enzymatic marker for the organelle. We show, however, that the genome of the microsporidian Nosema locustae, in contrast to that of E. cuniculi, encodes a group II large-subunit catalase. Surprisingly, phylogenetic analyses indicate that the N. locustae catalase is not specifically related to fungal homologs, as one would expect, but is instead closely related to proteobacterial sequences. This finding indicates that the N. locustae catalase is derived by lateral gene transfer from a bacterium. The catalase gene is adjacent to a large region of the genome that appears to be far less compact than is typical of microsporidian genomes, a characteristic which may make this region more amenable to the insertion of foreign genes. The N. locustae catalase gene is expressed in spores, and the protein is detectable by Western blotting. This type of catalase is a particularly robust enzyme that has been shown to function in dormant cells, indicating that the N. locustae catalase may play some functional role in the spore. There is no evidence that the N. locustae catalase functions in a cryptic peroxisome.

  • Bacterial catalase in the microsporidian Nosema locustae: implications for microsporidian metabolism and genome evolution. Eukaryot. Cell 2:1069–1075
    2003
    Co-Authors: Naomi M Fast, Bryony A P Williams, Joyce S Law, Patrick J Keeling
    Abstract:

    Microsporidia constitute a group of extremely specialized intracellular parasites that infect virtually all animals. They are highly derived, reduced fungi that lack several features typical of other eukaryotes, including canonical mitochondria, flagella, and peroxisomes. Consistent with the absence of peroxisomes in microspo-ridia, the recently completed genome of the microsporidian Encephalitozoon cuniculi lacks a gene for catalase, the major enzymatic marker for the organelle. We show, however, that the genome of the microsporidian Nosema locustae, in contrast to that of E. cuniculi, encodes a group II large-subunit catalase. Surprisingly, phylogenetic analyses indicate that the N. locustae catalase is not specifically related to fungal homologs, as one would expect, but is instead closely related to proteobacterial sequences. This finding indicates that the N. locustae catalase is derived by lateral gene transfer from a bacterium. The catalase gene is adjacent to a large region of the genome that appears to be far less compact than is typical of microsporidian genomes, a characteristic which may make this region more amenable to the insertion of foreign genes. The N. locustae catalase gene is expressed in spores, and the protein is detectable by Western blotting. This type of catalase is a particularly robust enzyme that has been shown to function in dormant cells, indicating that the N. locustae catalase may play some functional role in the spore. There is no evidence that the N. locustae catalase functions in a cryptic peroxisome

  • alpha and beta subunits of pyruvate dehydrogenase e1 from the microsporidian Nosema locustae mitochondrion derived carbon metabolism in microsporidia
    2001
    Co-Authors: Naomi M Fast, Patrick J Keeling
    Abstract:

    Microsporidia are highly adapted eukaryotic intracellular parasites that infect a variety of animals. Microsporidia contain no recognisable mitochondrion, but recently have been shown to have evolved from fungi and to possess heat shock protein genes derived from mitochondria. These findings make it clear that microsporidian ancestors were mitochondrial, yet it remains unknown whether they still contain the organelle, and if so what its role in microsporidian metabolism might be. Here we have characterised genes encoding the alpha and beta subunits of pyruvate dehydrogenase complex E1 (PDH, EC 1.2.4.1) from the microsporidian Nosema locustae. All other amitochondriate eukaryotes studied to date have lost the PDH complex and replaced it with pyruvate:ferredoxin oxidoreductase (PFOR). Nevertheless, molecular phylogeny shows that these Nosema enzymes are most closely related to mitochondrial PDH from other eukaryotes, demonstrating that elements of mitochondrial metabolism have been retained in microsporidia, and that PDH has not been wholly lost. However, there is still no evidence for a mitochondrion in microsporidia, and neither PDH subunit is predicted to encode an amino terminal leader sequence that could function as a mitochondrion-targeting transit peptide, raising questions as to whether these proteins function in a relic organelle or in the cytosol. Moreover, it is also unclear whether these proteins remain part of the PDH complex, or whether they have been retained for another purpose. We propose that microsporidia may utilise a unique pyruvate decarboxylation pathway involving PDH, demonstrating once again the diversity of core metabolism in amitochondriate eukaryotes.

  • Alpha and beta subunits of pyruvate dehydrogenase E1 from the microsporidian Nosema locustae: mitochondrionderived carbon metabolism in microsporidia.
    2001
    Co-Authors: Naomi M Fast, Patrick J Keeling
    Abstract:

    Abstract Microsporidia are highly adapted eukaryotic intracellular parasites that infect a variety of animals. Microsporidia contain no recognisable mitochondrion, but recently have been shown to have evolved from fungi and to possess heat shock protein genes derived from mitochondria. These findings make it clear that microsporidian ancestors were mitochondrial, yet it remains unknown whether they still contain the organelle, and if so what its role in microsporidian metabolism might be. Here we have characterised genes encoding the alpha and beta subunits of pyruvate dehydrogenase complex E1 (PDH, EC 1.2.4.1) from the microsporidian Nosema locustae. All other amitochondriate eukaryotes studied to date have lost the PDH complex and replaced it with pyruvate:ferredoxin oxidoreductase (PFOR). Nevertheless, molecular phylogeny shows that these Nosema enzymes are most closely related to mitochondrial PDH from other eukaryotes, demonstrating that elements of mitochondrial metabolism have been retained in microsporidia, and that PDH has not been wholly lost. However, there is still no evidence for a mitochondrion in microsporidia, and neither PDH subunit is predicted to encode an amino terminal leader sequence that could function as a mitochondrion-targeting transit peptide, raising questions as to whether these proteins function in a relic organelle or in the cytosol. Moreover, it is also unclear whether these proteins remain part of the PDH complex, or whether they have been retained for another purpose. We propose that microsporidia may utilise a unique pyruvate decarboxylation pathway involving PDH, demonstrating once again the diversity of core metabolism in amitochondriate eukaryotes

Lange, Carlos Ernesto - One of the best experts on this subject based on the ideXlab platform.

  • Susceptibilidad de la langosta Schistocerca cancellata (Orthoptera: Acrididae) a Nosema locustae (Protozoa: Microspora), en laboratorio
    1998
    Co-Authors: Wittenstein E., Sánchez, Norma Elba, Lange, Carlos Ernesto
    Abstract:

    The objective of this study was to determine the susceptibility of Schistocerca cancellata (Serville) to the pathogen Nosema locustae Canning, with the purpose of evaluating alternative control strategies of this pest. Third-instar nymphs of the gregarious phase of cultured S. cancellata were individually, per os challenged with 105 and 106 spores/nymph of N. locustae, a microbial agent for grasshopper control. Infection development occurred in 90.2 % of the nymphs inoculated (n = 112) and there was no difference in the percentage of infection between doses. There was a direct effect of dosage of N. locustae on mortality of S. cancellata, at 20 and 30 days postinoculation. Both doses had significantly higher percentages of mortality than that of the control. The higher dose caused distinctly greater mortality than the lower, with cumulative mortality at the end of the experiment being of 86% and 50%, respectively. None of the infected locusts reached adulthood. If these results are corroborated under natural conditions, N. locustae could be of value for the biological control of S. cancellata.El presente estudio tuvo como objetivo determinar la susceptibilidad de Schistocerca cancellata (Serville) al patógeno Nosema locustae Canning, a fin de evaluar posibles alternativas de su control biológico. Ninfas de tercer estadío de la fase gregaria de S. cancellata de bioterio fueron individualmente inoculadas per os con 105 y 106 esporos/ninfa de N. locustae, un agente microbiano para el control de acridios. El 90,2 % de las ninfas inoculadas (n = 112) desarrolló infección, no existiendo diferencias significativas en el porcentaje de ninfas infectadas entre ambas dosis ensayadas. La mortalidad de las ninfas no fue independiente de la dosis del patógeno, tanto a los 20 como a los 30 días postinoculación. Ambas dosis produjeron porcentajes de mortalidad significativamente más altos que el control. A su vez, la mortalidad acumulada al finalizar la experiencia fue significativamente más alta con la dosis mayor (86%) que con la dosis menor (50%). Ninguna de las langostas infectadas llegó al estado adulto. De ser corroborados estos resultados en condiciones naturales, N. locustae podría ser de utilidad para el control biológico de S. cancellata

  • Susceptibility of the locust Schistocerca cancellata (Orthoptera: Acrididae) to Nosema locustae (Protozoa: Microspora) in the laboratory
    1998
    Co-Authors: Lange, Carlos Ernesto, Wittenstein E., Sánchez, Norma Elba
    Abstract:

    El presente estudio tuvo como objetivo determinar la susceptibilidad de Schistocerca cancellata (Serville) al patógeno Nosema locustae Canning, a fin de evaluar posibles alternativas de su control biológico. Ninfas de tercer estadío de la fase gregaria de S. cancellata de bioterio fueron individualmente inoculadas "per os" con 105 y 106 esporos/ninfa de N. locustae, un agente microbiano para el control de acridios. El 90,2 % de las ninfas inoculadas (n = 112) desarrolló infección, no existiendo diferencias significativas en el porcentaje de ninfas infectadas entre ambas dosis ensayadas. La mortalidad de las ninfas no fue independiente de la dosis del patógeno, tanto a los 20 como a los 30 días postinoculación. Ambas dosis produjeron porcentajes de mortalidad significativamente más altos que el control. A su vez, la mortalidad acumulada al finalizar la experiencia fue significativamente más alta con la dosis mayor (86%) que con la dosis menor (50%). Ninguna de las langostas infectadas llegó al estado adulto. De ser corroborados estos resultados en condiciones naturales, N. locustae podría ser de utilidad para el control biológico de S. cancellata.The objective of this study was to determine the susceptibility of Schistocerca cancellata (Serville) to the pathogen Nosema locustae Canning, with the purpose of evaluating alternative control strategies of this pest. Third-instar nymphs of the gregarious phase of cultured S. cancellata were individually, "per os" challenged with 105 and 106 spores/nymph of N. locustae, a microbial agent for grasshopper control. Infection development occurred in 90.2 % of the nymphs inoculated (n = 112) and there was no difference in the percentage of infection between doses. There was a direct effect of dosage of N. locustae on mortality of S. cancellata, at 20 and 30 days postinoculation. Both doses had significantly higher percentages of mortality than that of the control. The higher dose caused distinctly greater mortality than the lower, with cumulative mortality at the end of the experiment being of 86% and 50%, respectively. None of the infected locusts reached adulthood. If these results are corroborated under natural conditions, N. locustae could be of value for the biological control of S. cancellata.Facultad de Ciencias Agrarias y Forestale

Arun M Khurad - One of the best experts on this subject based on the ideXlab platform.

  • transovarial transmission of Nosema locustae microsporida Nosematidae in the migratory locust locusta migratoria migratorioides
    1995
    Co-Authors: S K Raina, S Das, M M Rai, Arun M Khurad
    Abstract:

    Nosema locustae, a microsporidian parasite of locusts and grasshoppers, was transovarially transmitted to the progeny of infectedLocusta migratoria reared for up to F14 generations. The mortality of infected progeny in each generation was higher than that of uninfected controls and ranged from 67.6% to 95.5%. Infected female survivors transmitted the microsporidium to the progeny via eggs. The developing eggs harboured vegetative stages ofN. locustae, and development of the microsporidium occurred during embryonation. Spores accumulated in the yolk and, after blastokinesis, both the yolk and the spores were enclosed in the midgut of the embryo. germinated spores infected the functional midgut epithelium and invaded internal tissues. The mortality of newly hatched instars was high when embryonic tissue had been infected during development.

Sánchez, Norma Elba - One of the best experts on this subject based on the ideXlab platform.

  • Susceptibilidad de la langosta Schistocerca cancellata (Orthoptera: Acrididae) a Nosema locustae (Protozoa: Microspora), en laboratorio
    1998
    Co-Authors: Wittenstein E., Sánchez, Norma Elba, Lange, Carlos Ernesto
    Abstract:

    The objective of this study was to determine the susceptibility of Schistocerca cancellata (Serville) to the pathogen Nosema locustae Canning, with the purpose of evaluating alternative control strategies of this pest. Third-instar nymphs of the gregarious phase of cultured S. cancellata were individually, per os challenged with 105 and 106 spores/nymph of N. locustae, a microbial agent for grasshopper control. Infection development occurred in 90.2 % of the nymphs inoculated (n = 112) and there was no difference in the percentage of infection between doses. There was a direct effect of dosage of N. locustae on mortality of S. cancellata, at 20 and 30 days postinoculation. Both doses had significantly higher percentages of mortality than that of the control. The higher dose caused distinctly greater mortality than the lower, with cumulative mortality at the end of the experiment being of 86% and 50%, respectively. None of the infected locusts reached adulthood. If these results are corroborated under natural conditions, N. locustae could be of value for the biological control of S. cancellata.El presente estudio tuvo como objetivo determinar la susceptibilidad de Schistocerca cancellata (Serville) al patógeno Nosema locustae Canning, a fin de evaluar posibles alternativas de su control biológico. Ninfas de tercer estadío de la fase gregaria de S. cancellata de bioterio fueron individualmente inoculadas per os con 105 y 106 esporos/ninfa de N. locustae, un agente microbiano para el control de acridios. El 90,2 % de las ninfas inoculadas (n = 112) desarrolló infección, no existiendo diferencias significativas en el porcentaje de ninfas infectadas entre ambas dosis ensayadas. La mortalidad de las ninfas no fue independiente de la dosis del patógeno, tanto a los 20 como a los 30 días postinoculación. Ambas dosis produjeron porcentajes de mortalidad significativamente más altos que el control. A su vez, la mortalidad acumulada al finalizar la experiencia fue significativamente más alta con la dosis mayor (86%) que con la dosis menor (50%). Ninguna de las langostas infectadas llegó al estado adulto. De ser corroborados estos resultados en condiciones naturales, N. locustae podría ser de utilidad para el control biológico de S. cancellata

  • Susceptibility of the locust Schistocerca cancellata (Orthoptera: Acrididae) to Nosema locustae (Protozoa: Microspora) in the laboratory
    1998
    Co-Authors: Lange, Carlos Ernesto, Wittenstein E., Sánchez, Norma Elba
    Abstract:

    El presente estudio tuvo como objetivo determinar la susceptibilidad de Schistocerca cancellata (Serville) al patógeno Nosema locustae Canning, a fin de evaluar posibles alternativas de su control biológico. Ninfas de tercer estadío de la fase gregaria de S. cancellata de bioterio fueron individualmente inoculadas "per os" con 105 y 106 esporos/ninfa de N. locustae, un agente microbiano para el control de acridios. El 90,2 % de las ninfas inoculadas (n = 112) desarrolló infección, no existiendo diferencias significativas en el porcentaje de ninfas infectadas entre ambas dosis ensayadas. La mortalidad de las ninfas no fue independiente de la dosis del patógeno, tanto a los 20 como a los 30 días postinoculación. Ambas dosis produjeron porcentajes de mortalidad significativamente más altos que el control. A su vez, la mortalidad acumulada al finalizar la experiencia fue significativamente más alta con la dosis mayor (86%) que con la dosis menor (50%). Ninguna de las langostas infectadas llegó al estado adulto. De ser corroborados estos resultados en condiciones naturales, N. locustae podría ser de utilidad para el control biológico de S. cancellata.The objective of this study was to determine the susceptibility of Schistocerca cancellata (Serville) to the pathogen Nosema locustae Canning, with the purpose of evaluating alternative control strategies of this pest. Third-instar nymphs of the gregarious phase of cultured S. cancellata were individually, "per os" challenged with 105 and 106 spores/nymph of N. locustae, a microbial agent for grasshopper control. Infection development occurred in 90.2 % of the nymphs inoculated (n = 112) and there was no difference in the percentage of infection between doses. There was a direct effect of dosage of N. locustae on mortality of S. cancellata, at 20 and 30 days postinoculation. Both doses had significantly higher percentages of mortality than that of the control. The higher dose caused distinctly greater mortality than the lower, with cumulative mortality at the end of the experiment being of 86% and 50%, respectively. None of the infected locusts reached adulthood. If these results are corroborated under natural conditions, N. locustae could be of value for the biological control of S. cancellata.Facultad de Ciencias Agrarias y Forestale

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