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Prachi Masih - One of the best experts on this subject based on the ideXlab platform.
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genetic divergence in natural populations of bronze featherback Notopterus Notopterus osteoglossiformes notopteridae from five indian rivers analyzed through mtdna atpase6 8 regions
Meta Gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus Notopterus. In all, 97 samples of N. Notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P < 0.05) of the total population was found significant. The results concluded that the polymorphism in ATPase6/8 gene is a potential marker that is important for determining genetic divergence of wild N. Notopterus populations. The findings reveal common ancestry of mahanadi population with the populations in rivers of Indo-Gangetic region. However, long evolutionary isolation must be responsible for the high genetic divergence between N. Notopterus in Mahanadi and other regions.
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Genetic divergence in natural populations of bronze featherback, Notopterus Notopterus (Osteoglossiformes: Notopteridae) from five Indian rivers, analyzed through mtDNA ATPase6/8 regions.
Meta gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus Notopterus. In all, 97 samples of N. Notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P
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characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback Notopterus Notopterus pallas 1769
Molecular Biology Reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus Notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. Notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. Notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. Notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. Notopterus.
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Characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback, Notopterus Notopterus (Pallas, 1769)
Molecular biology reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus Notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. Notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. Notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. Notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P
R. S. Kulkarni - One of the best experts on this subject based on the ideXlab platform.
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Histopathological Changes in the Kidney of the Freshwater Bronze Featherback Fish, Notopterus Notopterus (Pallas, 1769) Exposed to Profenofos
Asian Journal of Research in Zoology, 2019Co-Authors: Safiya Tazeen, R. S. KulkarniAbstract:The impact of profenofos, an organophosphate pesticide on the kidney of Indian freshwater teleost fish Notopterus Notopterus was evaluated. The experimental group was exposed to sublethal concentration of profenofos at 7, 14 and 21 days. Histopathological changes due to the toxicity of pesticide in experimental and control kidney of the fish were observed by killing the animal every 7, 14 and 21 days. The kidneys were fixed in Bouin’s solution, dehydrated in graded ethanol, infiltrated in xylene, sectioned at 4-6 µ and stained in haematoxylin and eosin. Haemorrhage, oedema, fragmented glomeruli and elongated kidney tubules in experimental fish, and no effect were however observed in control fishes. It is concluded that profenofos is significantly toxic to the fish.
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Histopathological Impact of Profenofos on Ovary of the Freshwater Fish Notopterus Notopterus
Asian Journal of Research in Zoology, 2018Co-Authors: Safiya Tazeen, R. S. KulkarniAbstract:The presence of pesticide in the environment, owing to extensive use in agriculture and their low degradation capacity, are the potential toxicological concern for fish. Histological studies have been extensively used as biomarker in the assessment of the health of fish exposed to the pesticides, both in the laboratory as well as in the field studies. In the present study adult freshwater teleost fish Notopterus Notopterus of nearly similar weight and length were exposed to sub lethal concentration of 0.7 pp LC50 of profenofos (Curacron 50% EC) during reproductive phase. For histological changes the treated fish groups were compared with the control group. Marked changes such as atretic follicles, degenerative changes in oocyte were observed.
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Available online at www.ilns.pl
2016Co-Authors: K. Ravikiran, R. S. KulkarniAbstract:Nucleic acid content in different tissues of the fish, N. Notopterus in relation to se
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Hematology of the freshwater fish, Notopterus Notopterus in relation to Physico-chemical characteristics of the water
International Letters of Natural Sciences, 2015Co-Authors: R. S. KulkarniAbstract:Hematological and some biochemical contents of the blood in relation to physico- chemical characteristics of the water have been studied in the fresh water fish, Notopterus Notopterus. The results indicated that normal values have been found in comparison to the studies carried out in other fishes. Hence, the fish Notopterus Notopterus is having favorable environmental temperature, pH and dissolved oxygen required for the fish to survive and regulate breeding activities.
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Haematological and blood biochemical changes in the fresh water fish, Notopterus Notopterus (pallas) exposed to acidic medium
International Letters of Natural Sciences, 2015Co-Authors: R. S. Kulkarni, Vinayak BaradAbstract:The present study was carried out to find out the changes induced by one of the stress conditions such as acidic medium exposure on some haematological and blood biochemical, parameters in the fresh water fish, Notopterus Notopterus. Increase in the haemoglobin and haematocrite indicate haemoconcentration in the fish blood. Glucose, alkaline phosphatase, sodium and calcium, protein, BUN, creatinine, triglycerides, enzymes such as SGOT and SGPT were decreased in the fish N.Notopterus exposed to acidic condition. The changes in the environmental factors such as acidic condition cause stress to the fish which may bring disturbance in the blood parameters effecting the survival of fish.
Arti Gupta - One of the best experts on this subject based on the ideXlab platform.
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genetic divergence in natural populations of bronze featherback Notopterus Notopterus osteoglossiformes notopteridae from five indian rivers analyzed through mtdna atpase6 8 regions
Meta Gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus Notopterus. In all, 97 samples of N. Notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P < 0.05) of the total population was found significant. The results concluded that the polymorphism in ATPase6/8 gene is a potential marker that is important for determining genetic divergence of wild N. Notopterus populations. The findings reveal common ancestry of mahanadi population with the populations in rivers of Indo-Gangetic region. However, long evolutionary isolation must be responsible for the high genetic divergence between N. Notopterus in Mahanadi and other regions.
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Genetic divergence in natural populations of bronze featherback, Notopterus Notopterus (Osteoglossiformes: Notopteridae) from five Indian rivers, analyzed through mtDNA ATPase6/8 regions.
Meta gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus Notopterus. In all, 97 samples of N. Notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P
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characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback Notopterus Notopterus pallas 1769
Molecular Biology Reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus Notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. Notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. Notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. Notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. Notopterus.
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Characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback, Notopterus Notopterus (Pallas, 1769)
Molecular biology reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus Notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. Notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. Notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. Notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P
Rajeev K. Singh - One of the best experts on this subject based on the ideXlab platform.
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Spatio-temporal variations in length-weight relationship and condition factor of two notopterids, Chitala chitala (Hamilton, 1822) and Notopterus Notopterus (Pallas, 1769)
Indian Journal of Fisheries, 2020Co-Authors: Rejani Chandran, Rajeev K. Singh, Achal Singh, Pradipta Paul, R. S. Sah, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, J. K. JenaAbstract:Length-weight relationship and condition factor of Chitala chitala (Hamilton, 1822) and Notopterus Notopterus (Pallas, 1769) was studied from 439 samples of C. chitala collected from 14 locations and 368 samples of N. Notopterus colleted from 19 locations in Indian rivers. The regression coefficient ranged from 2.24 (River Gomti) to 3.24 (River Son) in C. chitala and 2.34 (River Son) to 3.71 (River Krishna) in N. Notopterus. Fulton’s condition factor and relative condition factor were found to be greater for N. Notopterus than C. chitala in common sites of collection. Spatial variations indicated better biological behaviour for C. chitala in River Son and for N. Notopterus in River Chambal. The results of the study form important baseline information for developing sustainable management and conservation measures.
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genetic divergence in natural populations of bronze featherback Notopterus Notopterus osteoglossiformes notopteridae from five indian rivers analyzed through mtdna atpase6 8 regions
Meta Gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus Notopterus. In all, 97 samples of N. Notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P < 0.05) of the total population was found significant. The results concluded that the polymorphism in ATPase6/8 gene is a potential marker that is important for determining genetic divergence of wild N. Notopterus populations. The findings reveal common ancestry of mahanadi population with the populations in rivers of Indo-Gangetic region. However, long evolutionary isolation must be responsible for the high genetic divergence between N. Notopterus in Mahanadi and other regions.
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Genetic divergence in natural populations of bronze featherback, Notopterus Notopterus (Osteoglossiformes: Notopteridae) from five Indian rivers, analyzed through mtDNA ATPase6/8 regions.
Meta gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus Notopterus. In all, 97 samples of N. Notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P
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characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback Notopterus Notopterus pallas 1769
Molecular Biology Reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus Notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. Notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. Notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. Notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. Notopterus.
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Characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback, Notopterus Notopterus (Pallas, 1769)
Molecular biology reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus Notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. Notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. Notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. Notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P
Kuldeep K. Lal - One of the best experts on this subject based on the ideXlab platform.
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Spatio-temporal variations in length-weight relationship and condition factor of two notopterids, Chitala chitala (Hamilton, 1822) and Notopterus Notopterus (Pallas, 1769)
Indian Journal of Fisheries, 2020Co-Authors: Rejani Chandran, Rajeev K. Singh, Achal Singh, Pradipta Paul, R. S. Sah, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, J. K. JenaAbstract:Length-weight relationship and condition factor of Chitala chitala (Hamilton, 1822) and Notopterus Notopterus (Pallas, 1769) was studied from 439 samples of C. chitala collected from 14 locations and 368 samples of N. Notopterus colleted from 19 locations in Indian rivers. The regression coefficient ranged from 2.24 (River Gomti) to 3.24 (River Son) in C. chitala and 2.34 (River Son) to 3.71 (River Krishna) in N. Notopterus. Fulton’s condition factor and relative condition factor were found to be greater for N. Notopterus than C. chitala in common sites of collection. Spatial variations indicated better biological behaviour for C. chitala in River Son and for N. Notopterus in River Chambal. The results of the study form important baseline information for developing sustainable management and conservation measures.
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genetic divergence in natural populations of bronze featherback Notopterus Notopterus osteoglossiformes notopteridae from five indian rivers analyzed through mtdna atpase6 8 regions
Meta Gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus Notopterus. In all, 97 samples of N. Notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P < 0.05) of the total population was found significant. The results concluded that the polymorphism in ATPase6/8 gene is a potential marker that is important for determining genetic divergence of wild N. Notopterus populations. The findings reveal common ancestry of mahanadi population with the populations in rivers of Indo-Gangetic region. However, long evolutionary isolation must be responsible for the high genetic divergence between N. Notopterus in Mahanadi and other regions.
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Genetic divergence in natural populations of bronze featherback, Notopterus Notopterus (Osteoglossiformes: Notopteridae) from five Indian rivers, analyzed through mtDNA ATPase6/8 regions.
Meta gene, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi Masih, Braj Kishor Gupta, Rahasya Mani MishraAbstract:The present study characterized 842 bp fragment of mitochondrial ATP synthase 6 and 8 (ATPase6/8) genes in Notopterus Notopterus. In all, 97 samples of N. Notopterus were collected from five distant rivers; viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi representing 4 river basins in India. The analysis of variation revealed presence of 23 haplotypes in ATPase6/8 gene with haplotype diversity (Hd) of 0.899 and nucleotide diversity (π) of 0.00336. The within population variation which was 41.78% of the total variation of 58.22% was found among population. The Fst value of 0.582 (P
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characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback Notopterus Notopterus pallas 1769
Molecular Biology Reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus Notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. Notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. Notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. Notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P < 0.0001) and high FST (0.2205) over all loci indicated that the samples are not drawn from the same genepool. The identified microsatellite loci are promising for use in fine-scale population structure analysis of N. Notopterus.
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Characterization of polymorphic microsatellite markers and genetic diversity in wild bronze featherback, Notopterus Notopterus (Pallas, 1769)
Molecular biology reports, 2013Co-Authors: Arti Gupta, Rajeev K. Singh, Rajesh Kumar, Vindhya Mohindra, Kuldeep K. Lal, Peyush Punia, Rama S Sah, Rupesh K Luhariya, Arvind Kumar Dwivedi, Prachi MasihAbstract:Six polymorphic microsatellite DNA loci were identified in the primitive fish, bronze featherback, Notopterus Notopterus for the first time and demonstrated significant population genetic structure. Out of the six primers, one primer (NN90) was specific to N. Notopterus (microsatellite sequence within the RAG1 gene) and five primers were product of successful cross-species amplification. Sixty-four primers available from 3 fish species of order Osteoglossiformes and families Notopteridae and Osteoglossidae were tested to amplify homologous microsatellite loci in N. Notopterus. Fifteen primer pairs exhibited successful cross-priming PCR product. However, polymorphism was detected only at five loci. To assess the significance of these six loci (including NN90) in population genetic study, 215 samples of N. Notopterus from five rivers, viz Satluj, Gomti, Yamuna, Brahmaputra and Mahanadi were analyzed. The five sample sets displayed different diversity levels and observed heterozygosity ranged from 0.6036 to 0.7373. Significant genotype heterogeneity (P
