The Experts below are selected from a list of 27033 Experts worldwide ranked by ideXlab platform
Rashid Bashir - One of the best experts on this subject based on the ideXlab platform.
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Graphene nanopores for Nucleic Acid Analysis
2012 12th IEEE International Conference on Nanotechnology (IEEE-NANO), 2012Co-Authors: Jiwook Shim, Vita Solovyeva, David Estrada, Shouvik Banerjee, Jose A. Rivera, Eric Pop, Rashid BashirAbstract:Solid-state nanopores are being widely investigated as potential tools for DNA sequencing and early detection of diseases. We review our recent work in the formation of nanopores in graphene embedded in thin layers of dielectrics for sensitive, controllable, and low-noise detection of bio-molecules. We also discuss applications in medical diagnostics.
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nanopore sensors for Nucleic Acid Analysis
Nature Nanotechnology, 2011Co-Authors: Bala Murali Venkatesan, Rashid BashirAbstract:Recent advances suggest that nanopore-based sensors may be able to sequence the human genome for under $1,000. This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
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Solid-State Nanopore Sensors for Nucleic Acid Analysis
Nanopores, 2011Co-Authors: Bala Murali Venkatesan, Rashid BashirAbstract:Solid-state nanopores are nm sized apertures formed in thin synthetic membranes. These single molecule sensors have been used in a variety of biophysical and diagnostic applications and serve as a potential candidate in the development of cost-effective, next generation DNA sequencing technologies, critical to furthering our understanding of inheritance, individuality, disease and evolution. The versatility of solid-state nanopore technology allows for both interfacing with biological systems at the nano-scale as well as large scale VLSI integration promising reliable, affordable, mass producible biosensors with single molecule sensing capabilities. In addition, this technology allows for truly parallel, high throughput DNA and protein Analysis through the development of nanopore and micropore arrays in ultra-thin synthetic membranes. This chapter is focused on the development of solid-state nanopore sensors in synthetic membranes and the potential benefits and challenges associated with this technology. Biological nanopores, primarily α-hemolysin and the phi29 connector are also reviewed. We conclude with a detailed discussion on chemically modified solid-state nanopores. These surface functionalized nanopore sensors combine the stability and versatility of solid-state nanopores with the sensitivity and selectivity of biological nanopore systems and may play an important role in drug screening and medical diagnostics.
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Nanopore sensors for Nucleic Acid Analysis
Nature Nanotechnology, 2011Co-Authors: Bala Murali Venkatesan, Rashid BashirAbstract:Nanopore Analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
Dmitry M. Kolpashchikov - One of the best experts on this subject based on the ideXlab platform.
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Nucleic Acid Analysis Using Multifunctional Hybridization Sensors
Proceedings, 2017Co-Authors: Dmitry M. KolpashchikovAbstract:Hybridization of Nucleic Acid probes remains one of the most common strategies for sensing of [...]
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A universal split spinach aptamer (USSA) for Nucleic Acid Analysis and DNA computation
Chemical communications (Cambridge England), 2017Co-Authors: Nanami Kikuchi, Dmitry M. KolpashchikovAbstract:We demonstrate how a single universal spinach aptamer (USSA) probe can be used to detect multiple (potentially any) Nucleic Acid sequences. USSA can be used for cost-efficient and highly selective Analysis of even folded DNA and RNA analytes, as well as for the readout of outputs of DNA logic circuits.
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A Differential Fluorescent Receptor for Nucleic Acid Analysis
Chembiochem : a European journal of chemical biology, 2013Co-Authors: Hillary N. Bengtson, Dmitry M. KolpashchikovAbstract:Differential receptors use an array of sensors to recognize analytes. Each sensor in the array can recognize not one, but several analytes with different rates, so a single analyte triggers a response of several sensors in the array. The receptor thus produces a pattern of signals that is unique for each analyte, thereby enabling identification of a specific analyte by producing a "fingerprint" pattern. We applied this approach for the Analysis of DNA sequences of Mycobacterium tuberculosis strains that differ by single nucleotide substitutions in the 81-bp hot-spot region that imparts rifampin resistance. The technology takes advantage of the new multicomponent, selfassembling sensor, which produces a fluorescent signal in the presence of specific DNA sequences. A differential fluorescent receptor (DFR) contained an array of three such sensors and differentiated at least eight DNA sequences. The approach requires only one molecular-beacon-like fluorescent reporter, which can be used by all three sensors. The DFR developed in this study represents a cost-efficient alternative to molecular diagnostic technologies that use fluorescent hybridization probes.
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DNA nanotechnology for Nucleic Acid Analysis: DX motif-based sensor.
Chembiochem : a European journal of chemical biology, 2011Co-Authors: Dmitry M. Kolpashchikov, Yulia V. Gerasimova, Mohammad S. KhanAbstract:A sensor that fluoresces in the presence of specific Nucleic Acids was designed and characterized. The sensor uses a molecular beacon probe and three adaptor strands to form five-stranded associate, a DX-tile, with a specific analyte. The new sensor can be used as a highly selective and affordable tool for real-time Analysis of DNA and RNA.
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rna cleaving deoxyribozyme sensor for Nucleic Acid Analysis the limit of detection
ChemBioChem, 2010Co-Authors: Yulia V. Gerasimova, Evan M. Cornett, Dmitry M. KolpashchikovAbstract:Along with biocompatibility, chemical stability, and simplicity of structural prediction and modification, deoxyribozyme-based molecular sensors have the potential of an improved detection limit due to their ability to catalytically amplify signal. This study contributes to the understanding of the factors responsible for the limit of detection (LOD) of RNA-cleaving deoxyribozyme sensors. A new sensor that detects specific DNA/RNA sequences was designed from deoxyribozyme OA-II [Chiuman, W.; Li, Y. (2006) J. Mol. Biol. 357, 748-754]. The sensor architecture allows for a unique combination of high selectivity, low LOD and the convenience of fluorescent signal monitoring in homogeneous solution. The LOD of the sensor was found to be approximately 1.6 x 10(-10) M after 3 h of incubation. An equation that allows estimation of the lowest theoretical LOD using characteristics of parent deoxyribozymes and their fluorogenic substrates was derived and experimentally verified. According to the equation, "catalytically perfect" enzymes can serve as scaffolds for the design of sensors with the LOD not lower than approximately 2 x 10(-15) M after 3 h of incubation. A new value termed the detection efficiency (DE) is suggested as a time-independent characteristic of a sensor's sensitivity. The expressions for the theoretical LOD and DE can be used to evaluate Nucleic Acid and protein enzymes for their application as biosensing platforms.
Bala Murali Venkatesan - One of the best experts on this subject based on the ideXlab platform.
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nanopore sensors for Nucleic Acid Analysis
Nature Nanotechnology, 2011Co-Authors: Bala Murali Venkatesan, Rashid BashirAbstract:Recent advances suggest that nanopore-based sensors may be able to sequence the human genome for under $1,000. This article reviews the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
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Solid-State Nanopore Sensors for Nucleic Acid Analysis
Nanopores, 2011Co-Authors: Bala Murali Venkatesan, Rashid BashirAbstract:Solid-state nanopores are nm sized apertures formed in thin synthetic membranes. These single molecule sensors have been used in a variety of biophysical and diagnostic applications and serve as a potential candidate in the development of cost-effective, next generation DNA sequencing technologies, critical to furthering our understanding of inheritance, individuality, disease and evolution. The versatility of solid-state nanopore technology allows for both interfacing with biological systems at the nano-scale as well as large scale VLSI integration promising reliable, affordable, mass producible biosensors with single molecule sensing capabilities. In addition, this technology allows for truly parallel, high throughput DNA and protein Analysis through the development of nanopore and micropore arrays in ultra-thin synthetic membranes. This chapter is focused on the development of solid-state nanopore sensors in synthetic membranes and the potential benefits and challenges associated with this technology. Biological nanopores, primarily α-hemolysin and the phi29 connector are also reviewed. We conclude with a detailed discussion on chemically modified solid-state nanopores. These surface functionalized nanopore sensors combine the stability and versatility of solid-state nanopores with the sensitivity and selectivity of biological nanopore systems and may play an important role in drug screening and medical diagnostics.
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Nanopore sensors for Nucleic Acid Analysis
Nature Nanotechnology, 2011Co-Authors: Bala Murali Venkatesan, Rashid BashirAbstract:Nanopore Analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
Ying Fang - One of the best experts on this subject based on the ideXlab platform.
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Recent advances in nanopore-based Nucleic Acid Analysis and sequencing
Microchimica Acta, 2015Co-Authors: Jidong Shi, Junfeng Hou, Ying FangAbstract:Nanopore-based sequencing platforms are transforming the field of genomic science. This review (containing 116 references) highlights some recent progress on nanopore-based Nucleic Acid Analysis and sequencing. These studies are classified into three categories, biological, solid-state, and hybrid nanopores, according to their nanoporous materials. We begin with a brief description of the translocation-based detection mechanism of nanopores. Next, specific examples are given in nanopore-based Nucleic Acid Analysis and sequencing, with an emphasis on identifying strategies that can improve the resolution of nanopores. This review concludes with a discussion of future research directions that will advance the practical applications of nanopore technology.
Jidong Shi - One of the best experts on this subject based on the ideXlab platform.
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Recent advances in nanopore-based Nucleic Acid Analysis and sequencing
Microchimica Acta, 2015Co-Authors: Jidong Shi, Junfeng Hou, Ying FangAbstract:Nanopore-based sequencing platforms are transforming the field of genomic science. This review (containing 116 references) highlights some recent progress on nanopore-based Nucleic Acid Analysis and sequencing. These studies are classified into three categories, biological, solid-state, and hybrid nanopores, according to their nanoporous materials. We begin with a brief description of the translocation-based detection mechanism of nanopores. Next, specific examples are given in nanopore-based Nucleic Acid Analysis and sequencing, with an emphasis on identifying strategies that can improve the resolution of nanopores. This review concludes with a discussion of future research directions that will advance the practical applications of nanopore technology.
