The Experts below are selected from a list of 24402 Experts worldwide ranked by ideXlab platform
Antonio Tufaro - One of the best experts on this subject based on the ideXlab platform.
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Rapid Serological Assays and SARS-CoV-2 Real-Time Polymerase Chain Reaction Assays for the Detection of SARS-CoV-2: Comparative Study.
Journal of medical Internet research, 2020Co-Authors: Angelo Virgilio Paradiso, Simona De Summa, Daniela Loconsole, Vito Procacci, A. Sallustio, Francesca Centrone, Nicola Silvestris, Vito Cafagna, Giuseppe De Palma, Antonio TufaroAbstract:BACKGROUND Real-time polymerase chain reaction (RT-PCR) testing for the identification of viral Nucleic Acid is the current standard for the diagnosis of SARS-CoV-2 infection, but technical issues limit its utilization for large-scale screening. Serological immunoglobulin M (IgM)/IgG testing has been proposed as a useful tool for detecting SARS-CoV-2 exposure. OBJECTIVE The objective of our study was to compare the results of the rapid serological VivaDiag test for SARS-CoV-2-related IgM/IgG detection with those of the standard RT-PCR laboratory test for identifying SARS-CoV-2 Nucleic Acid. Methods We simultaneously performed both serological and molecular tests with a consecutive series of 191 symptomatic patients. The results provided by a new rapid serological colorimetric test for analyzing IgM/IgG expression were compared with those of RT-PCR testing for SARS-CoV-2 detection. RESULTS Of the 191 subjects, 70 (36.6%) tested positive for SARS-CoV-2 based on RT-PCR results, while 34 (17.3%) tested positive based on serological IgM/IgG expression. Additionally, 13 (6.8%) subjects tested positive based on serological test results, but also tested negative based on RT-PCR results. The rapid serological test had a sensitivity of 30% and a specificity of 89% compared to the standard RT-PCR assay. Interestingly, the performance of both assays improved 8 days after symptom appearance. After 10 days had passed since symptom appearance, the predictive value of the rapid serological test was higher than that of the standard molecular assay (proportion of positive results: 40% vs 20%). Multivariate analysis showed that age >58 years (P 15 days after symptom onset (P
Xiao Yanqun - One of the best experts on this subject based on the ideXlab platform.
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Discussion of the performance verification procedures of the real-time quantitative polymerase chain reaction for determination of virus Nucleic Acid
Journal of Molecular Diagnostics and Therapy, 2012Co-Authors: Xiao YanqunAbstract:Objective To discuss the reasonable performance verification procedures of real-time quantitative fluorescent polymerase chain reaction for determination of virus Nucleic Acid. Methods The performance verification procedures were applied according to the related documents published by Clinical and Laboratory Standards Institute(CLSI). The limits of quantification were verified by diluting the samples to which can not be tested. Results The within-run coefficient of variation(CV) of HBV-DNA were 4.28% and 2.08%, the total CV were 4.14% and 2.69% and the regression equation was y=1.1082x-0.5225; the correlation of linearity was 0.9976, the regression equation was y=0.9771x-0.0062, the linearity range was 1.05E3~1.05E8 and the limit of quantitation was 500 IU/mL. The within-run CV of HCV-RNA were 4.11% and 2.63%, the total CV were 5.15% and 3.41%, the regression equation was y=1.0075x-0.0662; the correlation of linearity was 0.9974,the regression equation was y=1.0479x-0.2594,the linearity range was 1.50E3~2.53E7 and the limit of quantitation was 1000 IU/mL. Conclusion It is necessary to run the performance verification procedures before using new tests in clinical laboratory. The performance verification procedures of quantitative PCR for testing Nucleic virus and the statistics Methods are selected on the basis of practical situations.
Yoong Min Chong - One of the best experts on this subject based on the ideXlab platform.
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real time reverse transcription loop mediated isothermal amplification for rapid detection of sars cov 2
PeerJ, 2020Co-Authors: Ilyiana Ismail, Nur Izati Mustapa, Afifah Hassan, Kalaiarasu M Peariasamy, Yoong Min ChongAbstract:Background Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) Methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of Nucleic Acid. Methods A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. Results This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.
Angelo Virgilio Paradiso - One of the best experts on this subject based on the ideXlab platform.
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Rapid Serological Assays and SARS-CoV-2 Real-Time Polymerase Chain Reaction Assays for the Detection of SARS-CoV-2: Comparative Study.
Journal of medical Internet research, 2020Co-Authors: Angelo Virgilio Paradiso, Simona De Summa, Daniela Loconsole, Vito Procacci, A. Sallustio, Francesca Centrone, Nicola Silvestris, Vito Cafagna, Giuseppe De Palma, Antonio TufaroAbstract:BACKGROUND Real-time polymerase chain reaction (RT-PCR) testing for the identification of viral Nucleic Acid is the current standard for the diagnosis of SARS-CoV-2 infection, but technical issues limit its utilization for large-scale screening. Serological immunoglobulin M (IgM)/IgG testing has been proposed as a useful tool for detecting SARS-CoV-2 exposure. OBJECTIVE The objective of our study was to compare the results of the rapid serological VivaDiag test for SARS-CoV-2-related IgM/IgG detection with those of the standard RT-PCR laboratory test for identifying SARS-CoV-2 Nucleic Acid. Methods We simultaneously performed both serological and molecular tests with a consecutive series of 191 symptomatic patients. The results provided by a new rapid serological colorimetric test for analyzing IgM/IgG expression were compared with those of RT-PCR testing for SARS-CoV-2 detection. RESULTS Of the 191 subjects, 70 (36.6%) tested positive for SARS-CoV-2 based on RT-PCR results, while 34 (17.3%) tested positive based on serological IgM/IgG expression. Additionally, 13 (6.8%) subjects tested positive based on serological test results, but also tested negative based on RT-PCR results. The rapid serological test had a sensitivity of 30% and a specificity of 89% compared to the standard RT-PCR assay. Interestingly, the performance of both assays improved 8 days after symptom appearance. After 10 days had passed since symptom appearance, the predictive value of the rapid serological test was higher than that of the standard molecular assay (proportion of positive results: 40% vs 20%). Multivariate analysis showed that age >58 years (P 15 days after symptom onset (P
Ilyiana Ismail - One of the best experts on this subject based on the ideXlab platform.
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real time reverse transcription loop mediated isothermal amplification for rapid detection of sars cov 2
PeerJ, 2020Co-Authors: Ilyiana Ismail, Nur Izati Mustapa, Afifah Hassan, Kalaiarasu M Peariasamy, Yoong Min ChongAbstract:Background Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) Methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of Nucleic Acid. Methods A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. Results This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.
