The Experts below are selected from a list of 82293 Experts worldwide ranked by ideXlab platform
Robert M. Hoffman - One of the best experts on this subject based on the ideXlab platform.
-
imaging exosome transfer from breast cancer cells to stroma at metastatic sites in orthotopic Nude Mouse models
Advanced Drug Delivery Reviews, 2013Co-Authors: Atsushi Suetsugu, Kimi Honma, Shigetoyo Saji, Hisataka Moriwaki, Takahiro Ochiya, Robert M. HoffmanAbstract:Abstract Exosomes play an important role in cell-to-cell communication to promote tumor metastasis. In order to image the fate of cancer-cell-derived exosomes in orthotopic Nude Mouse models of breast cancer, we used green fluorescent protein (GFP)-tagged CD63, which is a general marker of exosomes. Breast cancer cells transferred their own exosomes to other cancer cells and normal lung tissue cells in culture. In orthotopic Nude-Mouse models, breast cancer cells secreted exosomes into the tumor microenvironment. Tumor-derived exosomes were incorporated into tumor-associated cells as well as circulating in the blood of mice with breast cancer metastases. These results suggest that tumor-derived exosomes may contribute to forming a niche to promote tumor growth and metastasis. Our results demonstrate the usefulness of GFP imaging to investigate the role of exosomes in cancer metastasis.
-
fluorescence guided surgery of human colon cancer increases complete resection resulting in cures in an orthotopic Nude Mouse model
Journal of Surgical Research, 2013Co-Authors: Cristina A Metildi, Sharmeela Kaushal, Robert M. Hoffman, Cynthia S Snyder, Michael BouvetAbstract:Background We inquired if fluorescence-guided surgery (FGS) could improve surgical outcomes in fluorescent orthotopic Nude Mouse models of human colon cancer.
-
development of the transgenic cyan fluorescent protein cfp expressing Nude Mouse for technicolor cancer imaging
Journal of Cellular Biochemistry, 2009Co-Authors: Hop Tran S Cao, Meng Yang, Jose Reynoso, Robert M. Hoffman, Hiroaki Kimura, Sharmeela Kaushal, Cynthia S Snyder, Michael BouvetAbstract:A major goal for in vivo biology is to develop models which can express multiple colors of fluorescent proteins in order to image multiple processes simultaneously in real time. Towards this goal, the cyanofluorescent protein (CFP) Nude Mouse was developed by crossing non-transgenic Nude mice with the transgenic CK/ECFP Mouse in which the β-actin promoter drives expression of CFP in almost all tissues. In crosses between nu/nu CFP male mice and nu/+ CFP female mice, approximately 50% of the embryos fluoresced blue. In the CFP Nude mice, the pancreas and reproductive organs displayed the strongest fluorescent signals of all internal organs which vary in intensity. Orthotopic implantation of XPA-1 human pancreatic cancer cells expressing red fluorescent protein (RFP) or green fluorescent protein (GFP) in the nucleus and RFP in the cytoplasm was performed in female Nude CFP mice. Color-coded fluorescence imaging of these human pancreatic cancer cells implanted into bright flue fluorescent pancreas of the CFP Nude Mouse afforded novel insight into the interaction of the pancreatic tumor and the normal pancreas, in particular the strong desmoplastic reaction of the tumor. The naturally enhanced blue fluorescence of the pancreas in the CFP Mouse serves as an ideal background for color-coded imaging of the interaction of implanted cancer cells and the host. The CFP Nude Mouse will provide unique understanding of the critical interplay between the cancer cells and their microenvironment.
-
A transgenic red fluorescent protein-expressing Nude Mouse for color-coded imaging of the tumor microenvironment.
Journal of cellular biochemistry, 2009Co-Authors: Meng Yang, Jose Reynoso, Michael Bouvet, Robert M. HoffmanAbstract:The tumor microenvironment (TME) is critical for tumor growth and progression. We have previously developed color-coded imaging of the TME using a green fluorescent protein (GFP) transgenic Nude Mouse as a host. However, most donor sources of cell types appropriate for study in the TME are from mice expressing GFP. Therefore, a Nude Mouse expressing red fluorescent protein (RFP) would be an appropriate host for transplantation of GFP-expressing stromal cells as well as double-labeled cancer cells expressing GFP in the nucleus and RFP in the cytoplasm, thereby creating a three-color imaging model of the TME. The RFP Nude Mouse was obtained by crossing non-transgenic Nude mice with the transgenic C57/B6 Mouse in which the β-actin promoter drives RFP (DsRed2) expression in essentially all tissues. In crosses between nu/nu RFP male mice and nu/+ RFP female mice, the embryos fluoresced red. Approximately 50% of the offspring of these mice were RFP Nude mice. In the RFP Nude Mouse, the organs all brightly expressed RFP, including the heart, lungs, spleen, pancreas, esophagus, stomach, duodenum, the male and female reproductive systems; brain and spinal cord; and the circulatory system, including the heart, and major arteries and veins. The skinned skeleton highly expressed RFP. The bone marrow and spleen cells were also RFP positive. GFP-expressing human cancer cell lines, including HCT-116-GFP colon cancer and MDA-MB-435-GFP breast cancer were orthotopically transplanted to the transgenic RFP Nude mice. These human tumors grew extensively in the transgenic RFP Nude Mouse. Dual-color fluorescence imaging enabled visualization of human tumor–host interaction. The RFP Nude Mouse model should greatly expand our knowledge of the TME. J. Cell. Biochem. 106: 279–284, 2009. © 2008 Wiley-Liss, Inc.
-
transgenic Nude Mouse with ubiquitous green fluorescent protein expression as a host for human tumors
Cancer Research, 2004Co-Authors: Meng Yang, Jose Reynoso, Ping Jiang, Abdool R Moossa, Robert M. HoffmanAbstract:We report here the development of the transgenic green fluorescent protein (GFP) Nude Mouse with ubiquitous GFP expression. The GFP Nude Mouse was obtained by crossing nontransgenic Nude mice with the transgenic C57/B6 Mouse in which the beta-actin promoter drives GFP expression in essentially all tissues. In crosses between nu/nu GFP male mice and nu/+ GFP female mice, the embryos fluoresced green. Approximately 50% of the offspring of these mice were GFP Nude mice. Newborn mice and adult mice fluoresced very bright green and could be detected with a simple blue-light-emitting diode flashlight with a central peak of 470 nm and a bypass emission filter. In the adult mice, the organs all brightly expressed GFP, including the heart, lungs, spleen, pancreas, esophagus, stomach, and duodenum. The following systems were dissected out and shown to have brilliant GFP fluorescence: the entire digestive system from tongue to anus; the male and female reproductive systems; brain and spinal cord; and the circulatory system, including the heart and major arteries and veins. The skinned skeleton highly expressed GFP. Pancreatic islets showed GFP fluorescence. The spleen cells were also GFP positive. Red fluorescent protein (RFP)-expressing human cancer cell lines, including PC-3-RFP prostate cancer, HCT-116-RFP colon cancer, MDA-MB-435-RFP breast cancer, and HT1080-RFP fibrosarcoma were transplanted to the transgenic GFP Nude mice. All of these human tumors grew extensively in the transgenic GFP Nude Mouse. Dual-color fluorescence imaging enabled visualization of human tumor-host interaction by whole-body imaging and at the cellular level in fresh and frozen tissues. The GFP Mouse model should greatly expand our knowledge of human tumor-host interaction.
Meng Yang - One of the best experts on this subject based on the ideXlab platform.
-
development of the transgenic cyan fluorescent protein cfp expressing Nude Mouse for technicolor cancer imaging
Journal of Cellular Biochemistry, 2009Co-Authors: Hop Tran S Cao, Meng Yang, Jose Reynoso, Robert M. Hoffman, Hiroaki Kimura, Sharmeela Kaushal, Cynthia S Snyder, Michael BouvetAbstract:A major goal for in vivo biology is to develop models which can express multiple colors of fluorescent proteins in order to image multiple processes simultaneously in real time. Towards this goal, the cyanofluorescent protein (CFP) Nude Mouse was developed by crossing non-transgenic Nude mice with the transgenic CK/ECFP Mouse in which the β-actin promoter drives expression of CFP in almost all tissues. In crosses between nu/nu CFP male mice and nu/+ CFP female mice, approximately 50% of the embryos fluoresced blue. In the CFP Nude mice, the pancreas and reproductive organs displayed the strongest fluorescent signals of all internal organs which vary in intensity. Orthotopic implantation of XPA-1 human pancreatic cancer cells expressing red fluorescent protein (RFP) or green fluorescent protein (GFP) in the nucleus and RFP in the cytoplasm was performed in female Nude CFP mice. Color-coded fluorescence imaging of these human pancreatic cancer cells implanted into bright flue fluorescent pancreas of the CFP Nude Mouse afforded novel insight into the interaction of the pancreatic tumor and the normal pancreas, in particular the strong desmoplastic reaction of the tumor. The naturally enhanced blue fluorescence of the pancreas in the CFP Mouse serves as an ideal background for color-coded imaging of the interaction of implanted cancer cells and the host. The CFP Nude Mouse will provide unique understanding of the critical interplay between the cancer cells and their microenvironment.
-
A transgenic red fluorescent protein-expressing Nude Mouse for color-coded imaging of the tumor microenvironment.
Journal of cellular biochemistry, 2009Co-Authors: Meng Yang, Jose Reynoso, Michael Bouvet, Robert M. HoffmanAbstract:The tumor microenvironment (TME) is critical for tumor growth and progression. We have previously developed color-coded imaging of the TME using a green fluorescent protein (GFP) transgenic Nude Mouse as a host. However, most donor sources of cell types appropriate for study in the TME are from mice expressing GFP. Therefore, a Nude Mouse expressing red fluorescent protein (RFP) would be an appropriate host for transplantation of GFP-expressing stromal cells as well as double-labeled cancer cells expressing GFP in the nucleus and RFP in the cytoplasm, thereby creating a three-color imaging model of the TME. The RFP Nude Mouse was obtained by crossing non-transgenic Nude mice with the transgenic C57/B6 Mouse in which the β-actin promoter drives RFP (DsRed2) expression in essentially all tissues. In crosses between nu/nu RFP male mice and nu/+ RFP female mice, the embryos fluoresced red. Approximately 50% of the offspring of these mice were RFP Nude mice. In the RFP Nude Mouse, the organs all brightly expressed RFP, including the heart, lungs, spleen, pancreas, esophagus, stomach, duodenum, the male and female reproductive systems; brain and spinal cord; and the circulatory system, including the heart, and major arteries and veins. The skinned skeleton highly expressed RFP. The bone marrow and spleen cells were also RFP positive. GFP-expressing human cancer cell lines, including HCT-116-GFP colon cancer and MDA-MB-435-GFP breast cancer were orthotopically transplanted to the transgenic RFP Nude mice. These human tumors grew extensively in the transgenic RFP Nude Mouse. Dual-color fluorescence imaging enabled visualization of human tumor–host interaction. The RFP Nude Mouse model should greatly expand our knowledge of the TME. J. Cell. Biochem. 106: 279–284, 2009. © 2008 Wiley-Liss, Inc.
-
transgenic Nude Mouse with ubiquitous green fluorescent protein expression as a host for human tumors
Cancer Research, 2004Co-Authors: Meng Yang, Jose Reynoso, Ping Jiang, Abdool R Moossa, Robert M. HoffmanAbstract:We report here the development of the transgenic green fluorescent protein (GFP) Nude Mouse with ubiquitous GFP expression. The GFP Nude Mouse was obtained by crossing nontransgenic Nude mice with the transgenic C57/B6 Mouse in which the beta-actin promoter drives GFP expression in essentially all tissues. In crosses between nu/nu GFP male mice and nu/+ GFP female mice, the embryos fluoresced green. Approximately 50% of the offspring of these mice were GFP Nude mice. Newborn mice and adult mice fluoresced very bright green and could be detected with a simple blue-light-emitting diode flashlight with a central peak of 470 nm and a bypass emission filter. In the adult mice, the organs all brightly expressed GFP, including the heart, lungs, spleen, pancreas, esophagus, stomach, and duodenum. The following systems were dissected out and shown to have brilliant GFP fluorescence: the entire digestive system from tongue to anus; the male and female reproductive systems; brain and spinal cord; and the circulatory system, including the heart and major arteries and veins. The skinned skeleton highly expressed GFP. Pancreatic islets showed GFP fluorescence. The spleen cells were also GFP positive. Red fluorescent protein (RFP)-expressing human cancer cell lines, including PC-3-RFP prostate cancer, HCT-116-RFP colon cancer, MDA-MB-435-RFP breast cancer, and HT1080-RFP fibrosarcoma were transplanted to the transgenic GFP Nude mice. All of these human tumors grew extensively in the transgenic GFP Nude Mouse. Dual-color fluorescence imaging enabled visualization of human tumor-host interaction by whole-body imaging and at the cellular level in fresh and frozen tissues. The GFP Mouse model should greatly expand our knowledge of human tumor-host interaction.
Michael Bouvet - One of the best experts on this subject based on the ideXlab platform.
-
fluorescence guided surgery of human colon cancer increases complete resection resulting in cures in an orthotopic Nude Mouse model
Journal of Surgical Research, 2013Co-Authors: Cristina A Metildi, Sharmeela Kaushal, Robert M. Hoffman, Cynthia S Snyder, Michael BouvetAbstract:Background We inquired if fluorescence-guided surgery (FGS) could improve surgical outcomes in fluorescent orthotopic Nude Mouse models of human colon cancer.
-
development of the transgenic cyan fluorescent protein cfp expressing Nude Mouse for technicolor cancer imaging
Journal of Cellular Biochemistry, 2009Co-Authors: Hop Tran S Cao, Meng Yang, Jose Reynoso, Robert M. Hoffman, Hiroaki Kimura, Sharmeela Kaushal, Cynthia S Snyder, Michael BouvetAbstract:A major goal for in vivo biology is to develop models which can express multiple colors of fluorescent proteins in order to image multiple processes simultaneously in real time. Towards this goal, the cyanofluorescent protein (CFP) Nude Mouse was developed by crossing non-transgenic Nude mice with the transgenic CK/ECFP Mouse in which the β-actin promoter drives expression of CFP in almost all tissues. In crosses between nu/nu CFP male mice and nu/+ CFP female mice, approximately 50% of the embryos fluoresced blue. In the CFP Nude mice, the pancreas and reproductive organs displayed the strongest fluorescent signals of all internal organs which vary in intensity. Orthotopic implantation of XPA-1 human pancreatic cancer cells expressing red fluorescent protein (RFP) or green fluorescent protein (GFP) in the nucleus and RFP in the cytoplasm was performed in female Nude CFP mice. Color-coded fluorescence imaging of these human pancreatic cancer cells implanted into bright flue fluorescent pancreas of the CFP Nude Mouse afforded novel insight into the interaction of the pancreatic tumor and the normal pancreas, in particular the strong desmoplastic reaction of the tumor. The naturally enhanced blue fluorescence of the pancreas in the CFP Mouse serves as an ideal background for color-coded imaging of the interaction of implanted cancer cells and the host. The CFP Nude Mouse will provide unique understanding of the critical interplay between the cancer cells and their microenvironment.
-
A transgenic red fluorescent protein-expressing Nude Mouse for color-coded imaging of the tumor microenvironment.
Journal of cellular biochemistry, 2009Co-Authors: Meng Yang, Jose Reynoso, Michael Bouvet, Robert M. HoffmanAbstract:The tumor microenvironment (TME) is critical for tumor growth and progression. We have previously developed color-coded imaging of the TME using a green fluorescent protein (GFP) transgenic Nude Mouse as a host. However, most donor sources of cell types appropriate for study in the TME are from mice expressing GFP. Therefore, a Nude Mouse expressing red fluorescent protein (RFP) would be an appropriate host for transplantation of GFP-expressing stromal cells as well as double-labeled cancer cells expressing GFP in the nucleus and RFP in the cytoplasm, thereby creating a three-color imaging model of the TME. The RFP Nude Mouse was obtained by crossing non-transgenic Nude mice with the transgenic C57/B6 Mouse in which the β-actin promoter drives RFP (DsRed2) expression in essentially all tissues. In crosses between nu/nu RFP male mice and nu/+ RFP female mice, the embryos fluoresced red. Approximately 50% of the offspring of these mice were RFP Nude mice. In the RFP Nude Mouse, the organs all brightly expressed RFP, including the heart, lungs, spleen, pancreas, esophagus, stomach, duodenum, the male and female reproductive systems; brain and spinal cord; and the circulatory system, including the heart, and major arteries and veins. The skinned skeleton highly expressed RFP. The bone marrow and spleen cells were also RFP positive. GFP-expressing human cancer cell lines, including HCT-116-GFP colon cancer and MDA-MB-435-GFP breast cancer were orthotopically transplanted to the transgenic RFP Nude mice. These human tumors grew extensively in the transgenic RFP Nude Mouse. Dual-color fluorescence imaging enabled visualization of human tumor–host interaction. The RFP Nude Mouse model should greatly expand our knowledge of the TME. J. Cell. Biochem. 106: 279–284, 2009. © 2008 Wiley-Liss, Inc.
Jose Reynoso - One of the best experts on this subject based on the ideXlab platform.
-
development of the transgenic cyan fluorescent protein cfp expressing Nude Mouse for technicolor cancer imaging
Journal of Cellular Biochemistry, 2009Co-Authors: Hop Tran S Cao, Meng Yang, Jose Reynoso, Robert M. Hoffman, Hiroaki Kimura, Sharmeela Kaushal, Cynthia S Snyder, Michael BouvetAbstract:A major goal for in vivo biology is to develop models which can express multiple colors of fluorescent proteins in order to image multiple processes simultaneously in real time. Towards this goal, the cyanofluorescent protein (CFP) Nude Mouse was developed by crossing non-transgenic Nude mice with the transgenic CK/ECFP Mouse in which the β-actin promoter drives expression of CFP in almost all tissues. In crosses between nu/nu CFP male mice and nu/+ CFP female mice, approximately 50% of the embryos fluoresced blue. In the CFP Nude mice, the pancreas and reproductive organs displayed the strongest fluorescent signals of all internal organs which vary in intensity. Orthotopic implantation of XPA-1 human pancreatic cancer cells expressing red fluorescent protein (RFP) or green fluorescent protein (GFP) in the nucleus and RFP in the cytoplasm was performed in female Nude CFP mice. Color-coded fluorescence imaging of these human pancreatic cancer cells implanted into bright flue fluorescent pancreas of the CFP Nude Mouse afforded novel insight into the interaction of the pancreatic tumor and the normal pancreas, in particular the strong desmoplastic reaction of the tumor. The naturally enhanced blue fluorescence of the pancreas in the CFP Mouse serves as an ideal background for color-coded imaging of the interaction of implanted cancer cells and the host. The CFP Nude Mouse will provide unique understanding of the critical interplay between the cancer cells and their microenvironment.
-
A transgenic red fluorescent protein-expressing Nude Mouse for color-coded imaging of the tumor microenvironment.
Journal of cellular biochemistry, 2009Co-Authors: Meng Yang, Jose Reynoso, Michael Bouvet, Robert M. HoffmanAbstract:The tumor microenvironment (TME) is critical for tumor growth and progression. We have previously developed color-coded imaging of the TME using a green fluorescent protein (GFP) transgenic Nude Mouse as a host. However, most donor sources of cell types appropriate for study in the TME are from mice expressing GFP. Therefore, a Nude Mouse expressing red fluorescent protein (RFP) would be an appropriate host for transplantation of GFP-expressing stromal cells as well as double-labeled cancer cells expressing GFP in the nucleus and RFP in the cytoplasm, thereby creating a three-color imaging model of the TME. The RFP Nude Mouse was obtained by crossing non-transgenic Nude mice with the transgenic C57/B6 Mouse in which the β-actin promoter drives RFP (DsRed2) expression in essentially all tissues. In crosses between nu/nu RFP male mice and nu/+ RFP female mice, the embryos fluoresced red. Approximately 50% of the offspring of these mice were RFP Nude mice. In the RFP Nude Mouse, the organs all brightly expressed RFP, including the heart, lungs, spleen, pancreas, esophagus, stomach, duodenum, the male and female reproductive systems; brain and spinal cord; and the circulatory system, including the heart, and major arteries and veins. The skinned skeleton highly expressed RFP. The bone marrow and spleen cells were also RFP positive. GFP-expressing human cancer cell lines, including HCT-116-GFP colon cancer and MDA-MB-435-GFP breast cancer were orthotopically transplanted to the transgenic RFP Nude mice. These human tumors grew extensively in the transgenic RFP Nude Mouse. Dual-color fluorescence imaging enabled visualization of human tumor–host interaction. The RFP Nude Mouse model should greatly expand our knowledge of the TME. J. Cell. Biochem. 106: 279–284, 2009. © 2008 Wiley-Liss, Inc.
-
transgenic Nude Mouse with ubiquitous green fluorescent protein expression as a host for human tumors
Cancer Research, 2004Co-Authors: Meng Yang, Jose Reynoso, Ping Jiang, Abdool R Moossa, Robert M. HoffmanAbstract:We report here the development of the transgenic green fluorescent protein (GFP) Nude Mouse with ubiquitous GFP expression. The GFP Nude Mouse was obtained by crossing nontransgenic Nude mice with the transgenic C57/B6 Mouse in which the beta-actin promoter drives GFP expression in essentially all tissues. In crosses between nu/nu GFP male mice and nu/+ GFP female mice, the embryos fluoresced green. Approximately 50% of the offspring of these mice were GFP Nude mice. Newborn mice and adult mice fluoresced very bright green and could be detected with a simple blue-light-emitting diode flashlight with a central peak of 470 nm and a bypass emission filter. In the adult mice, the organs all brightly expressed GFP, including the heart, lungs, spleen, pancreas, esophagus, stomach, and duodenum. The following systems were dissected out and shown to have brilliant GFP fluorescence: the entire digestive system from tongue to anus; the male and female reproductive systems; brain and spinal cord; and the circulatory system, including the heart and major arteries and veins. The skinned skeleton highly expressed GFP. Pancreatic islets showed GFP fluorescence. The spleen cells were also GFP positive. Red fluorescent protein (RFP)-expressing human cancer cell lines, including PC-3-RFP prostate cancer, HCT-116-RFP colon cancer, MDA-MB-435-RFP breast cancer, and HT1080-RFP fibrosarcoma were transplanted to the transgenic GFP Nude mice. All of these human tumors grew extensively in the transgenic GFP Nude Mouse. Dual-color fluorescence imaging enabled visualization of human tumor-host interaction by whole-body imaging and at the cellular level in fresh and frozen tissues. The GFP Mouse model should greatly expand our knowledge of human tumor-host interaction.
Fiorella Guadagni - One of the best experts on this subject based on the ideXlab platform.
-
A metastatic Nude-Mouse model of human pancreatic cancer constructed orthotopically with histologically intact patient specimens.
Proceedings of the National Academy of Sciences of the United States of America, 1992Co-Authors: Fiorella Guadagni, Robert M. HoffmanAbstract:Abstract Pancreatic cancer is one of the most intractable and least understood of all human cancers. Pancreatic cancers is the fourth-leading cause of cancer-related mortality in the United States with less than 2% of the patients surviving for 5 yr. In an effort to help develop more effective treatment modalities for pancreatic cancer and improve detection, we report an animal model for individual human pancreatic-cancer patients. The model involves orthotopic transplantation of histologically intact pancreatic-cancer specimens to the Nude-Mouse pancreas, which can result in models that resemble the clinical picture including (i) extensive local tumor growth, (ii) extension of the locally growing human pancreatic cancer to the Nude-Mouse stomach and duodenum, (iii) metastases of the human pancreatic tumor to the Nude-Mouse liver and regional lymph nodes, and (iv) distant metastases of the human pancreatic tumor to the Nude-Mouse adrenal gland, diaphragm, and mediastinal lymph nodes. In a series of five patient cases, a 100% take rate has been demonstrated, and of 17 mice transplanted, 15 supported tumor growth. Immunohistochemical analysis of the antigenic phenotype of the transplanted human pancreatic tumors showed a similar pattern of expression of two different human tumor-associated antigens, such as tumor-associated glycoprotein 72 and carcinoembryonic antigen in the transplanted tumors when compared with the original surgical biopsy, suggesting similarity between the two. This model should, therefore, prove valuable for treatment evaluation of individual cancer patients, as well as for evaluation of experimental treatment modalities for this disease.
