The Experts below are selected from a list of 15621 Experts worldwide ranked by ideXlab platform
Cock Van Oosterhout - One of the best experts on this subject based on the ideXlab platform.
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estimation and adjustment of microsatellite Null Alleles in nonequilibrium populations
Molecular Ecology Notes, 2006Co-Authors: Cock Van Oosterhout, David Weetman, William F. HutchinsonAbstract:Nonamplified (Null) Alleles are a common feature of microsatellite genotyping and can bias estimates of allele and genotype frequencies, thereby hindering population genetic analyses. The frequency of microsatellite Null Alleles in diploid populations can be estimated for populations that are in Hardy–Weinberg equilibrium. However, many microsatellite data sets are from nonequilibrium populations, often with known inbreeding coefficients ( F ) or fixation indices ( F IS or F ST ). Here, we propose a novel Null allele estimator that can be used to estimate the Null allele frequency and adjust visible allele frequencies in populations for which independent estimates of F , F IS or F ST are available. The algorithm is currently available as an Excel macro that can be downloaded at no cost from http://www.microchecker. hull.ac.uk/ and will be incorporated into the software MICRO - CHECKER .
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micro checker software for identifying and correcting genotyping errors in microsatellite data
Molecular Ecology Notes, 2004Co-Authors: Cock Van Oosterhout, William F. Hutchinson, Derek P. M. Wills, Peter ShipleyAbstract:DNA degradation, low DNA concentrations and primer-site mutations may result in the incorrect assignment of microsatellite genotypes, potentially biasing population genetic analyses. MICRO - CHECKER is WINDOWS ®-based software that tests the genotyping of microsatellites from diploid populations. The program aids identification of genotyping errors due to nonamplified Alleles (Null Alleles), short allele dominance (large allele dropout) and the scoring of stutter peaks, and also detects typographic errors. MICRO - CHECKER estimates the frequency of Null Alleles and, importantly, can adjust the allele and genotype frequencies of the amplified Alleles, permitting their use in further population genetic analysis. MICRO CHECKER can be freely downloaded from http://www.microchecker.hull.ac.uk/.
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micro checker software for identifying and correcting genotyping errors in microsatellite data
Molecular Ecology Notes, 2004Co-Authors: Cock Van Oosterhout, William F. Hutchinson, Derek P. M. Wills, Peter ShipleyAbstract:DNA degradation, low DNA concentrations and primer-site mutations may result in the incorrect assignment of microsatellite genotypes, potentially biasing population genetic analyses. MICRO - CHECKER is WINDOWS ®-based software that tests the genotyping of microsatellites from diploid populations. The program aids identification of genotyping errors due to nonamplified Alleles (Null Alleles), short allele dominance (large allele dropout) and the scoring of stutter peaks, and also detects typographic errors. MICRO - CHECKER estimates the frequency of Null Alleles and, importantly, can adjust the allele and genotype frequencies of the amplified Alleles, permitting their use in further population genetic analysis. MICRO CHECKER can be freely downloaded from http://www.microchecker.hull.ac.uk/.
William F. Hutchinson - One of the best experts on this subject based on the ideXlab platform.
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estimation and adjustment of microsatellite Null Alleles in nonequilibrium populations
Molecular Ecology Notes, 2006Co-Authors: Cock Van Oosterhout, David Weetman, William F. HutchinsonAbstract:Nonamplified (Null) Alleles are a common feature of microsatellite genotyping and can bias estimates of allele and genotype frequencies, thereby hindering population genetic analyses. The frequency of microsatellite Null Alleles in diploid populations can be estimated for populations that are in Hardy–Weinberg equilibrium. However, many microsatellite data sets are from nonequilibrium populations, often with known inbreeding coefficients ( F ) or fixation indices ( F IS or F ST ). Here, we propose a novel Null allele estimator that can be used to estimate the Null allele frequency and adjust visible allele frequencies in populations for which independent estimates of F , F IS or F ST are available. The algorithm is currently available as an Excel macro that can be downloaded at no cost from http://www.microchecker. hull.ac.uk/ and will be incorporated into the software MICRO - CHECKER .
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micro checker software for identifying and correcting genotyping errors in microsatellite data
Molecular Ecology Notes, 2004Co-Authors: Cock Van Oosterhout, William F. Hutchinson, Derek P. M. Wills, Peter ShipleyAbstract:DNA degradation, low DNA concentrations and primer-site mutations may result in the incorrect assignment of microsatellite genotypes, potentially biasing population genetic analyses. MICRO - CHECKER is WINDOWS ®-based software that tests the genotyping of microsatellites from diploid populations. The program aids identification of genotyping errors due to nonamplified Alleles (Null Alleles), short allele dominance (large allele dropout) and the scoring of stutter peaks, and also detects typographic errors. MICRO - CHECKER estimates the frequency of Null Alleles and, importantly, can adjust the allele and genotype frequencies of the amplified Alleles, permitting their use in further population genetic analysis. MICRO CHECKER can be freely downloaded from http://www.microchecker.hull.ac.uk/.
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micro checker software for identifying and correcting genotyping errors in microsatellite data
Molecular Ecology Notes, 2004Co-Authors: Cock Van Oosterhout, William F. Hutchinson, Derek P. M. Wills, Peter ShipleyAbstract:DNA degradation, low DNA concentrations and primer-site mutations may result in the incorrect assignment of microsatellite genotypes, potentially biasing population genetic analyses. MICRO - CHECKER is WINDOWS ®-based software that tests the genotyping of microsatellites from diploid populations. The program aids identification of genotyping errors due to nonamplified Alleles (Null Alleles), short allele dominance (large allele dropout) and the scoring of stutter peaks, and also detects typographic errors. MICRO - CHECKER estimates the frequency of Null Alleles and, importantly, can adjust the allele and genotype frequencies of the amplified Alleles, permitting their use in further population genetic analysis. MICRO CHECKER can be freely downloaded from http://www.microchecker.hull.ac.uk/.
Peter Shipley - One of the best experts on this subject based on the ideXlab platform.
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micro checker software for identifying and correcting genotyping errors in microsatellite data
Molecular Ecology Notes, 2004Co-Authors: Cock Van Oosterhout, William F. Hutchinson, Derek P. M. Wills, Peter ShipleyAbstract:DNA degradation, low DNA concentrations and primer-site mutations may result in the incorrect assignment of microsatellite genotypes, potentially biasing population genetic analyses. MICRO - CHECKER is WINDOWS ®-based software that tests the genotyping of microsatellites from diploid populations. The program aids identification of genotyping errors due to nonamplified Alleles (Null Alleles), short allele dominance (large allele dropout) and the scoring of stutter peaks, and also detects typographic errors. MICRO - CHECKER estimates the frequency of Null Alleles and, importantly, can adjust the allele and genotype frequencies of the amplified Alleles, permitting their use in further population genetic analysis. MICRO CHECKER can be freely downloaded from http://www.microchecker.hull.ac.uk/.
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micro checker software for identifying and correcting genotyping errors in microsatellite data
Molecular Ecology Notes, 2004Co-Authors: Cock Van Oosterhout, William F. Hutchinson, Derek P. M. Wills, Peter ShipleyAbstract:DNA degradation, low DNA concentrations and primer-site mutations may result in the incorrect assignment of microsatellite genotypes, potentially biasing population genetic analyses. MICRO - CHECKER is WINDOWS ®-based software that tests the genotyping of microsatellites from diploid populations. The program aids identification of genotyping errors due to nonamplified Alleles (Null Alleles), short allele dominance (large allele dropout) and the scoring of stutter peaks, and also detects typographic errors. MICRO - CHECKER estimates the frequency of Null Alleles and, importantly, can adjust the allele and genotype frequencies of the amplified Alleles, permitting their use in further population genetic analysis. MICRO CHECKER can be freely downloaded from http://www.microchecker.hull.ac.uk/.
Carole Saison - One of the best experts on this subject based on the ideXlab platform.
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Null Alleles of abcg2 encoding the breast cancer resistance protein define the new blood group system junior
Nature Genetics, 2012Co-Authors: Carole Saison, Virginie Helias, Bryan A Ballif, Thierry Peyrard, Herve Puy, Toru Miyazaki, Sebastien Perrot, Muriel Vayssiertaussat, Mauro Waldner, Pierreyves Le PennecAbstract:Lionel Arnaud and colleagues identify Null Alleles of ABCG2 as the genetic basis of the Junior blood group system.
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Null Alleles of ABCG2 encoding the breast cancer resistance protein define the new blood group system Junior
Nature Genetics, 2012Co-Authors: Carole Saison, Virginie Helias, Bryan A Ballif, Thierry Peyrard, Herve Puy, Sebastien Perrot, Muriel Taussat, Pierre Yves Le Pennec, Jean Pierre Cartron, Lionel ArnaudAbstract:The breast cancer resistance protein, also known as ABCG2, is one of the most highly studied ATP-binding cassette (ABC) transporters because of its ability to confer multidrug resistance. The lack of information on the physiological role of ABCG2 in humans severely limits cancer chemotherapeutic approaches targeting this transporter. We report here that ABCG2 comprises the molecular basis of a new blood group system (Junior, Jr) and that individuals of the Jr(a-) blood type have inherited two Null Alleles of ABCG2. We identified five frameshift and three nonsense mutations in ABCG2. We also show that the prevalence of the Jr(a-) blood type in the Japanese and European Gypsy populations is related to the p.Gln126* and p.Arg236* protein alterations, respectively. The identification of ABCG2(-/-) (Jr(a-)) individuals who appear phenotypically normal is an essential step toward targeting ABCG2 in cancer and also in understanding the physiological and pharmacological roles of this promiscuous transporter in humans.
Akihiro Kijima - One of the best experts on this subject based on the ideXlab platform.
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inheritance of microsatellite dna markers in the pacific abalone haliotis discus hannai
Marine Biotechnology, 2003Co-Authors: Choulji Park, Toshimasa Kobayashi, Akihiro KijimaAbstract:Microsatellite markers have been developed for a variety of abalones, and locus-specific homozygote excesses at population level have been recorded for microsatellite loci. To ascertain whether Null Alleles exist at microsatellite loci in the Pacific abalone, we studied the mode of inheritance of 7 microsatellite loci in 4 families with a reciprocal cross of 2 females x 2 males. All loci segregated codominantly, but only 3 loci ( Hdh1321, Hdh78, and Hdd108C) conformed to Mendelian segregation and can be used for parental analysis and population genetic studies. When Null Alleles were considered, 2 loci (Hdh1761 and Hdh1457) confirmed Mendelian expectations in all families, while the remaining 2 loci (Hdd114B and Hdd229) showed deviation from Mendelian segregation in at least one family even though Null Alleles were considered. These results indicated the need to test the inheritance pattern for microsatellite markers in abalones before using them for population genetic of parentage analysis.
