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Yin-hui Leong - One of the best experts on this subject based on the ideXlab platform.

  • associations of serum aflatoxin b1 lysine adduct level with socio demographic factors and aflatoxins intake from Nuts and related Nut Products in malaysia
    International Journal of Hygiene and Environmental Health, 2012
    Co-Authors: Yin-hui Leong, Ahmad Rosma, Aishah A. Latiff, Nurul A Izzah
    Abstract:

    Abstract Aflatoxins are one of the major risk factors in the multi-factorial etiology of human hepatocellular carcinoma. Therefore, the information on aflatoxins exposure is very important in the intervention planning in order to reduce the dietary intake of aflatoxins, especially among the children. This study investigated the relationship between aflatoxin B 1 (AFB 1 ) lysine adduct levers in serum and socio-demographic factors and dietary intake of aflatoxins from Nuts and Nut Products in Penang, Malaysia. A cross-sectional field study was conducted in five districts of Penang. A survey on socio-demographic characteristics was administered to 364 healthy adults from the three main ethnic groups (Malay, Chinese and Indian). A total of 170 blood samples were successfully collected and tested for the level of AFB 1 –lysine adduct. 97% of the samples contained AFB 1 –lysine adduct above the detection limit of 0.4 pg/mg albumin and ranged from 0.20 to 23.16 pg/mg albumin (mean ± standard deviation = 7.67 ± 4.54 pg/mg albumin; median = 7.12 pg/mg albumin). There was no significant association between AFB 1 –lysine adduct levels with gender, district, education level, household number and occupation when these socio-demographic characteristics were examined according to high or low levels of AFB 1 –lysine. However, participants in the age group of 31–50 years were 3.08 times more likely to have high AFB 1 levels compared to those aged between 18 and 30 years ( P  = 0.026). Significant difference ( P  = 0.000) was found among different ethnic groups. Chinese and Indian participants were 3.05 and 2.35 times more likely to have high AFB 1 levels than Malay. The result of AFB 1 –lysine adduct suggested that Penang adult population is likely to be exposed to AFB 1 but at a level of less than that needed to cause direct acute illness or death.

  • Associations of serum aflatoxin B1–lysine adduct level with socio-demographic factors and aflatoxins intake from Nuts and related Nut Products in Malaysia
    International journal of hygiene and environmental health, 2012
    Co-Authors: Yin-hui Leong, Ahmad Rosma, Aishah A. Latiff, A. Nurul Izzah
    Abstract:

    Abstract Aflatoxins are one of the major risk factors in the multi-factorial etiology of human hepatocellular carcinoma. Therefore, the information on aflatoxins exposure is very important in the intervention planning in order to reduce the dietary intake of aflatoxins, especially among the children. This study investigated the relationship between aflatoxin B 1 (AFB 1 ) lysine adduct levers in serum and socio-demographic factors and dietary intake of aflatoxins from Nuts and Nut Products in Penang, Malaysia. A cross-sectional field study was conducted in five districts of Penang. A survey on socio-demographic characteristics was administered to 364 healthy adults from the three main ethnic groups (Malay, Chinese and Indian). A total of 170 blood samples were successfully collected and tested for the level of AFB 1 –lysine adduct. 97% of the samples contained AFB 1 –lysine adduct above the detection limit of 0.4 pg/mg albumin and ranged from 0.20 to 23.16 pg/mg albumin (mean ± standard deviation = 7.67 ± 4.54 pg/mg albumin; median = 7.12 pg/mg albumin). There was no significant association between AFB 1 –lysine adduct levels with gender, district, education level, household number and occupation when these socio-demographic characteristics were examined according to high or low levels of AFB 1 –lysine. However, participants in the age group of 31–50 years were 3.08 times more likely to have high AFB 1 levels compared to those aged between 18 and 30 years ( P  = 0.026). Significant difference ( P  = 0.000) was found among different ethnic groups. Chinese and Indian participants were 3.05 and 2.35 times more likely to have high AFB 1 levels than Malay. The result of AFB 1 –lysine adduct suggested that Penang adult population is likely to be exposed to AFB 1 but at a level of less than that needed to cause direct acute illness or death.

  • Exposure assessment and risk characterization of aflatoxin B_1 in Malaysia
    Mycotoxin Research, 2011
    Co-Authors: Yin-hui Leong, Ahmad Rosma, Aishah A. Latiff, Nurul Izzah Ahmad
    Abstract:

    Aflatoxin B_1 (AFB_1) was detected in 57% of the Nuts and Nut Products marketed in Penang, Malaysia using the liquid chromatography tandem mass spectrometry. The contamination levels ranged from 0.40 to 222 μg/kg and 17 out of 128 samples (13.3%) contained AFB_1 above the European Commission permitted level (2 μg/kg). Estimated dietary exposure of AFB_1 in Nuts and Nut Products were 0.36 ng per kg body weight and day and 8.89 ng per kg body weight and day, representing the low and high-level of exposure, respectively. Dose-response modelling resulted in benchmark dose lower confidence limit (BMDL_10) values of 0.305 ng per kg body weight and day, with the best fitted from the log-logistic model. The derived margin of exposure (MoE) values ranged from 34 to 847 suggested that AFB_1 would be of public health concern and might reasonably be considered as a high priority for risk management actions.

  • Exposure assessment and risk characterization of aflatoxin B1 in Malaysia.
    Mycotoxin research, 2011
    Co-Authors: Yin-hui Leong, Ahmad Rosma, Aishah A. Latiff, Nurul Izzah Ahmad
    Abstract:

    Aflatoxin B1 (AFB1) was detected in 57% of the Nuts and Nut Products marketed in Penang, Malaysia using the liquid chromatography tandem mass spectrometry. The contamination levels ranged from 0.40 to 222 μg/kg and 17 out of 128 samples (13.3%) contained AFB1 above the European Commission permitted level (2 μg/kg). Estimated dietary exposure of AFB1 in Nuts and Nut Products were 0.36 ng per kg body weight and day and 8.89 ng per kg body weight and day, representing the low and high-level of exposure, respectively. Dose-response modelling resulted in benchmark dose lower confidence limit (BMDL10) values of 0.305 ng per kg body weight and day, with the best fitted from the log-logistic model. The derived margin of exposure (MoE) values ranged from 34 to 847 suggested that AFB1 would be of public health concern and might reasonably be considered as a high priority for risk management actions.

  • Determination of aflatoxins in commercial Nuts and Nut Products using liquid chromatography tandem mass spectrometry
    World Mycotoxin Journal, 2011
    Co-Authors: Yin-hui Leong, Aishah A. Latiff, Nor Azliza Ismail, Normaliza Abdul Manaf, A. Rosma
    Abstract:

    A rapid and sensitive confirmatory analytical method for determination of aflatoxins in Nuts and their Products using liquid chromatography tandem mass spectrometry has been developed and validated. All four aflatoxins of interest (B1, B2, G1 and G2) were quantified using aflatoxin M1 as the internal standard. Samples were extracted using methanol/water (60:40, v/v) and cleaned-up with immunoaffinity column. Limit of detection ranged between 0.10 and 0.30 µg/kg. Recovery rates were between 76 and 105% for all analytes. For further validation, a reference material for contaminated peaNut has been used for quality assurance measures to check the accuracy and precision. The method was successfully applied to determine the natural occurrence of aflatoxins in 128 Nut samples marketed in Penang, Malaysia. More than half of the samples (57.0%) were positive and 13.3% were found to be non-compliant with the European Commission regulations permitted maximum level of 2 µg/kg for aflatoxin B1. The contamination leve...

James Chang - One of the best experts on this subject based on the ideXlab platform.

Chiading Liao - One of the best experts on this subject based on the ideXlab platform.

  • multi mycotoxin analysis of finished grain and Nut Products using ultrahigh performance liquid chromatography and positive electrospray ionization quadrupole orbital ion trap high resolution mass spectrometry
    Journal of Agricultural and Food Chemistry, 2015
    Co-Authors: Chiading Liao, Jon W Wong, Kai Zhang, Paul Yang, James B Wittenberg, Mary W Trucksess, Douglas G Hayward, Nathaniel S Lee, James Chang
    Abstract:

    Ultrahigh-performance liquid chromatography using positive electrospray ionization and quadrupole orbital ion trap high-resolution mass spectrometry was evaluated for analyzing mycotoxins in finished cereal and Nut Products. Optimizing the orbital ion trap mass analyzer in full-scan mode using mycotoxin-fortified matrix extracts gave mass accuracies, δM, of <±2.0 ppm at 70 000 full width at half maximum (FWHM) mass resolution (RFWHM). The limits of quantitation were matrix- and mycotoxin-dependent, ranging from 0.02 to 11.6 μg/kg. Mean recoveries and standard deviations for mycotoxins from acetonitrile/water extraction at their relevant fortification levels were 91 ± 10, 94 ± 10, 98 ± 12, 91 ± 13, 99 ± 15, and 93 ± 17% for corn, rice, wheat, almond, peaNut, and pistachio, respectively. Nineteen mycotoxins with concentrations ranging from 0.3 (aflatoxin B1 in peaNut and almond) to 1175 μg/kg (fumonisin B1 in corn flour) were found in 35 of the 70 commercial grain and Nut samples surveyed. Mycotoxins could ...

  • Multi-mycotoxin Analysis of Finished Grain and Nut Products Using Ultrahigh-Performance Liquid Chromatography and Positive Electrospray Ionization-Quadrupole Orbital Ion Trap High-Resolution Mass Spectrometry.
    Journal of agricultural and food chemistry, 2015
    Co-Authors: Chiading Liao, Jon W Wong, Kai Zhang, Paul Yang, James B Wittenberg, Mary W Trucksess, Douglas G Hayward, Nathaniel S Lee, James Chang
    Abstract:

    Ultrahigh-performance liquid chromatography using positive electrospray ionization and quadrupole orbital ion trap high-resolution mass spectrometry was evaluated for analyzing mycotoxins in finished cereal and Nut Products. Optimizing the orbital ion trap mass analyzer in full-scan mode using mycotoxin-fortified matrix extracts gave mass accuracies, δM, of

  • multi mycotoxin analysis of finished grain and Nut Products using high performance liquid chromatography triple quadrupole mass spectrometry
    Journal of Agricultural and Food Chemistry, 2013
    Co-Authors: Chiading Liao, Jon W Wong, Kai Zhang, Douglas G Hayward, Nathaniel S Lee, Mary W Trucksess
    Abstract:

    Mycotoxins in foods have long been recognized as potential health hazards due to their toxic and carcinogenic properties. A simple and rapid method was developed to detect 26 mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, and ergot alkaloids) in corn, rice, wheat, almond, peaNut, and pistachio Products using high-performance liquid chromatography-triple-quadrupole mass spectrometry. Test portions of homogenized grain or Nut Products were extracted with acetonitrile/water (85:15, v/v), followed by high-speed centrifugation and dilution with water. Mean recoveries (± standard deviations) were 84 ± 6, 89 ± 6, 97 ± 9, 87 ± 12, 104 ± 16, and 92 ± 18% from corn, rice, wheat, almond, peaNut, and pistachio Products, respectively, and the matrix-dependent instrument quantitation limits ranged from 0.2 to 12.8 μg/kg, depending on the mycotoxin. Matrix effects, as measured by the slope ratios of matrix-matched and solvent-only calibration curves, revealed primarily suppression and were more pronounced in Nuts than in grains. The measured mycotoxin concentrations in 11 corn and wheat reference materials were not different from the certified concentrations. Nineteen mycotoxins were identified and measured in 35 of 70 commercial grain and Nut Products, ranging from 0.3 ± 0.1 μg/kg (aflatoxin B1 in peaNuts) to 1143 ± 87 μg/kg (fumonisin B1 in corn flour). This rapid and efficient method was shown to be rugged and effective for the multiresidue analysis of mycotoxins in finished grain and Nut Products.

Mary W Trucksess - One of the best experts on this subject based on the ideXlab platform.

  • multi mycotoxin analysis of finished grain and Nut Products using ultrahigh performance liquid chromatography and positive electrospray ionization quadrupole orbital ion trap high resolution mass spectrometry
    Journal of Agricultural and Food Chemistry, 2015
    Co-Authors: Chiading Liao, Jon W Wong, Kai Zhang, Paul Yang, James B Wittenberg, Mary W Trucksess, Douglas G Hayward, Nathaniel S Lee, James Chang
    Abstract:

    Ultrahigh-performance liquid chromatography using positive electrospray ionization and quadrupole orbital ion trap high-resolution mass spectrometry was evaluated for analyzing mycotoxins in finished cereal and Nut Products. Optimizing the orbital ion trap mass analyzer in full-scan mode using mycotoxin-fortified matrix extracts gave mass accuracies, δM, of <±2.0 ppm at 70 000 full width at half maximum (FWHM) mass resolution (RFWHM). The limits of quantitation were matrix- and mycotoxin-dependent, ranging from 0.02 to 11.6 μg/kg. Mean recoveries and standard deviations for mycotoxins from acetonitrile/water extraction at their relevant fortification levels were 91 ± 10, 94 ± 10, 98 ± 12, 91 ± 13, 99 ± 15, and 93 ± 17% for corn, rice, wheat, almond, peaNut, and pistachio, respectively. Nineteen mycotoxins with concentrations ranging from 0.3 (aflatoxin B1 in peaNut and almond) to 1175 μg/kg (fumonisin B1 in corn flour) were found in 35 of the 70 commercial grain and Nut samples surveyed. Mycotoxins could ...

  • Multi-mycotoxin Analysis of Finished Grain and Nut Products Using Ultrahigh-Performance Liquid Chromatography and Positive Electrospray Ionization-Quadrupole Orbital Ion Trap High-Resolution Mass Spectrometry.
    Journal of agricultural and food chemistry, 2015
    Co-Authors: Chiading Liao, Jon W Wong, Kai Zhang, Paul Yang, James B Wittenberg, Mary W Trucksess, Douglas G Hayward, Nathaniel S Lee, James Chang
    Abstract:

    Ultrahigh-performance liquid chromatography using positive electrospray ionization and quadrupole orbital ion trap high-resolution mass spectrometry was evaluated for analyzing mycotoxins in finished cereal and Nut Products. Optimizing the orbital ion trap mass analyzer in full-scan mode using mycotoxin-fortified matrix extracts gave mass accuracies, δM, of

  • multi mycotoxin analysis of finished grain and Nut Products using high performance liquid chromatography triple quadrupole mass spectrometry
    Journal of Agricultural and Food Chemistry, 2013
    Co-Authors: Chiading Liao, Jon W Wong, Kai Zhang, Douglas G Hayward, Nathaniel S Lee, Mary W Trucksess
    Abstract:

    Mycotoxins in foods have long been recognized as potential health hazards due to their toxic and carcinogenic properties. A simple and rapid method was developed to detect 26 mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, and ergot alkaloids) in corn, rice, wheat, almond, peaNut, and pistachio Products using high-performance liquid chromatography-triple-quadrupole mass spectrometry. Test portions of homogenized grain or Nut Products were extracted with acetonitrile/water (85:15, v/v), followed by high-speed centrifugation and dilution with water. Mean recoveries (± standard deviations) were 84 ± 6, 89 ± 6, 97 ± 9, 87 ± 12, 104 ± 16, and 92 ± 18% from corn, rice, wheat, almond, peaNut, and pistachio Products, respectively, and the matrix-dependent instrument quantitation limits ranged from 0.2 to 12.8 μg/kg, depending on the mycotoxin. Matrix effects, as measured by the slope ratios of matrix-matched and solvent-only calibration curves, revealed primarily suppression and were more pronounced in Nuts than in grains. The measured mycotoxin concentrations in 11 corn and wheat reference materials were not different from the certified concentrations. Nineteen mycotoxins were identified and measured in 35 of 70 commercial grain and Nut Products, ranging from 0.3 ± 0.1 μg/kg (aflatoxin B1 in peaNuts) to 1143 ± 87 μg/kg (fumonisin B1 in corn flour). This rapid and efficient method was shown to be rugged and effective for the multiresidue analysis of mycotoxins in finished grain and Nut Products.

Douglas G Hayward - One of the best experts on this subject based on the ideXlab platform.

  • multi mycotoxin analysis of finished grain and Nut Products using ultrahigh performance liquid chromatography and positive electrospray ionization quadrupole orbital ion trap high resolution mass spectrometry
    Journal of Agricultural and Food Chemistry, 2015
    Co-Authors: Chiading Liao, Jon W Wong, Kai Zhang, Paul Yang, James B Wittenberg, Mary W Trucksess, Douglas G Hayward, Nathaniel S Lee, James Chang
    Abstract:

    Ultrahigh-performance liquid chromatography using positive electrospray ionization and quadrupole orbital ion trap high-resolution mass spectrometry was evaluated for analyzing mycotoxins in finished cereal and Nut Products. Optimizing the orbital ion trap mass analyzer in full-scan mode using mycotoxin-fortified matrix extracts gave mass accuracies, δM, of <±2.0 ppm at 70 000 full width at half maximum (FWHM) mass resolution (RFWHM). The limits of quantitation were matrix- and mycotoxin-dependent, ranging from 0.02 to 11.6 μg/kg. Mean recoveries and standard deviations for mycotoxins from acetonitrile/water extraction at their relevant fortification levels were 91 ± 10, 94 ± 10, 98 ± 12, 91 ± 13, 99 ± 15, and 93 ± 17% for corn, rice, wheat, almond, peaNut, and pistachio, respectively. Nineteen mycotoxins with concentrations ranging from 0.3 (aflatoxin B1 in peaNut and almond) to 1175 μg/kg (fumonisin B1 in corn flour) were found in 35 of the 70 commercial grain and Nut samples surveyed. Mycotoxins could ...

  • Multi-mycotoxin Analysis of Finished Grain and Nut Products Using Ultrahigh-Performance Liquid Chromatography and Positive Electrospray Ionization-Quadrupole Orbital Ion Trap High-Resolution Mass Spectrometry.
    Journal of agricultural and food chemistry, 2015
    Co-Authors: Chiading Liao, Jon W Wong, Kai Zhang, Paul Yang, James B Wittenberg, Mary W Trucksess, Douglas G Hayward, Nathaniel S Lee, James Chang
    Abstract:

    Ultrahigh-performance liquid chromatography using positive electrospray ionization and quadrupole orbital ion trap high-resolution mass spectrometry was evaluated for analyzing mycotoxins in finished cereal and Nut Products. Optimizing the orbital ion trap mass analyzer in full-scan mode using mycotoxin-fortified matrix extracts gave mass accuracies, δM, of

  • multi mycotoxin analysis of finished grain and Nut Products using high performance liquid chromatography triple quadrupole mass spectrometry
    Journal of Agricultural and Food Chemistry, 2013
    Co-Authors: Chiading Liao, Jon W Wong, Kai Zhang, Douglas G Hayward, Nathaniel S Lee, Mary W Trucksess
    Abstract:

    Mycotoxins in foods have long been recognized as potential health hazards due to their toxic and carcinogenic properties. A simple and rapid method was developed to detect 26 mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, and ergot alkaloids) in corn, rice, wheat, almond, peaNut, and pistachio Products using high-performance liquid chromatography-triple-quadrupole mass spectrometry. Test portions of homogenized grain or Nut Products were extracted with acetonitrile/water (85:15, v/v), followed by high-speed centrifugation and dilution with water. Mean recoveries (± standard deviations) were 84 ± 6, 89 ± 6, 97 ± 9, 87 ± 12, 104 ± 16, and 92 ± 18% from corn, rice, wheat, almond, peaNut, and pistachio Products, respectively, and the matrix-dependent instrument quantitation limits ranged from 0.2 to 12.8 μg/kg, depending on the mycotoxin. Matrix effects, as measured by the slope ratios of matrix-matched and solvent-only calibration curves, revealed primarily suppression and were more pronounced in Nuts than in grains. The measured mycotoxin concentrations in 11 corn and wheat reference materials were not different from the certified concentrations. Nineteen mycotoxins were identified and measured in 35 of 70 commercial grain and Nut Products, ranging from 0.3 ± 0.1 μg/kg (aflatoxin B1 in peaNuts) to 1143 ± 87 μg/kg (fumonisin B1 in corn flour). This rapid and efficient method was shown to be rugged and effective for the multiresidue analysis of mycotoxins in finished grain and Nut Products.

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