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Maria Antonia Pedrine Colabone Celligoi - One of the best experts on this subject based on the ideXlab platform.
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assessment of a new edible film biodegradable based on starch Nystose to increase quality and the shelf life of blackberries
Food bioscience, 2021Co-Authors: Gabrielly Terassi Bersaneti, Suzana Mali, Sandra Helena Prudencio, Maria Antonia Pedrine Colabone CelligoiAbstract:Abstract Biodegradable edible films have been studied, because increase the shelf life of different foods that are highly perishable and, in addition, they carry on other compounds that improve their mechanical properties coating and add value to the final product. Therefore, in this study, was evaluated the effect of an edible prebiotic coating of starch with the addition of Nystose, with the objective to preserve the quality and increase the useful life of blackberries stored at 4 °C for 20 days. Control fruits and those coated only with starch showed higher counts for psychrotrophic microorganisms and molds and yeasts after 7 days and fruits with starch-Nystose coating contamination was reduced with counts recommended for consumption. The starch and starch-Nystose coatings were effective in delaying the increase in pH, maintaining the firmness and anthocyanin content of the fruits. The sensory evaluation showed that the fruits coated with the starch-Nystose film obtained good acceptance and purchase intention, without significant differences with the control fruit. These results are promising, the biodegradable starch coating with the addition of Nystose proved to efficient, preserving the quality of blackberry, and can be an innovative alternative to prolong the useful life of the fruits.
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evaluation of the prebiotic activities of edible starch films with the addition of Nystose from bacillus subtilis natto
Lwt - Food Science and Technology, 2019Co-Authors: Gabrielly Terassi Bersaneti, Sandra Garcia, Suzana Mali, Maria Antonia Pedrine Colabone CelligoiAbstract:Abstract Edible films and coatings have attracted industrial interest due to the ability to increase the shelf life of food products and the ability to contain bioactive ingredients to improve the nutritional quality of food. In the current study, we evaluated the prebiotic activities of Nystose synthesized by levansucrase from Bacillus subtilis and evaluated edible starch-Nystose as the sole source of energy for Bifidobacterium and Lactobacillus strains. The growth of the lactic acid bacteria and the formation of organic acids demonstrated that Nystose and the edible starch-Nystose film showed a prebiotic effect. The edible starch-Nystose film may exhibit a prebiotic function in addition to the protective coating effect. This biotechnology product demonstrates potential functional applications for the food industry and may be used for coating fresh fruits and vegetables as well as for the addition of the prebiotic Nystose.
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Utilization of low-cost substrates for the production of Nystose by Bacillus subtilis natto cct 7712
Editora da Universidade Estadual de Maringá, 2016Co-Authors: Dieyssi Alves Dos Santos, Cristiani Baldo, Dionísio Borsato, Maria Antonia Pedrine Colabone CelligoiAbstract:Current analysis describes the capacity of Bacillus subtilis natto CCT 7712 to produce high amounts of Nystose by low-cost substrates available in Brazil, such as commercial sucrose, sugarcane molasses and sugarcane juice. Optimization resulted in a maximum production of 179.77 g L-1 of Nystose, averaging 7.49 g L-1 hour-1 of productivity and a 71.73% yield in a medium with 400 g L-1of commercial sucrose and 0.8 g L-1 of MnSO4. Fermentations with sugarcane molasses and sugarcane juice also resulted in a satisfactory production reaching 97.93 and 42.58 g L-1 Nystose, respectively. High Nystose production in a medium with sugarcane derivatives suggests submerged fermentation with Bacillus subtillis natto CT 7712 as a promising strategy to produce Nystose at industrial level
Francisco J. Plou - One of the best experts on this subject based on the ideXlab platform.
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levan versus fructooligosaccharide synthesis using the levansucrase from zymomonas mobilis effect of reaction conditions
Journal of Molecular Catalysis B-enzymatic, 2015Co-Authors: Paloma Santosmoriano, Lucia Fernandezarrojo, Ana Poveda, Jesus Jimenezbarbero, Antonio Ballesteros, Francisco J. PlouAbstract:Abstract This work addresses the effect of sucrose concentration and temperature on the three activities displayed by the levansucrase from Zymomonas mobilis : formation of levan (polymerization), production of short-chain fructooligosaccharides (FOS), and sucrose hydrolysis. Of the conditions tested, levan formation reached the highest value at 4 °C and 100 g/L sucrose. The increase of temperature (40 °C) and sucrose concentration (600 g/L) caused a significant decrease of the levan concentration and a higher production of FOS. However, an increase of the temperature also caused an enhancement of the undesired hydrolytic activity. Several inulin-type FOS (1-kestose, Nystose, 1 F-fructosylNystose), neoFOS (blastose, neokestose, neoNystose) and levan-type FOS (6-kestose, 6,6-Nystose) were synthesized by levansucrase. The latter compound was purified and characterized by mass spectrometry and 2D NMR. Using 600 g/L sucrose at 40 °C, the maximum yield of FOS was reached at 85% sucrose conversion; at this point, the reaction mixture contained (in weight basis) 31% glucose, 14% fructose, 15% sucrose and 40% FOS (including a small contribution of levan).
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production of fructooligosaccharides by mycelium bound transfructosylation activity present in cladosporium cladosporioides and penicilium sizovae
Process Biochemistry, 2014Co-Authors: Paolo Zambelli, Lucia Fernandezarrojo, Maria Fernandezlobato, Diego Romano, Paloma Santosmoriano, M Gimenoperez, Ana Poveda, Raffaella Gandolfi, Francesco Molinari, Francisco J. PlouAbstract:Abstract Different filamentous fungi isolated from molasses and jams (kiwi and fig) were screened for fructooligosaccharides (FOS) producing activity. Two strains, identified as Penicilium sizovae (CK1) and Cladosporium cladosporioides (CF215), were selected on the basis of the FOS yield and kestose/Nystose ratio. In both strains the activity was mostly mycelium-bound. Starting from 600 g/L of sucrose, maximum FOS yield was 184 and 339 g/L for P. sizovae and C. cladosporioides, respectively. Interestingly, the highest FOS concentration with C. cladosporioides was reached at 93% sucrose conversion, which indicated a notable transglycosylation to hydrolysis ratio. The main FOS in the reaction mixtures were identified by HPAEC–PAD chromatography. C. cladosporioides synthesized mainly 1-kestose (158 g/L), Nystose (97 g/L), 1F-fructosylNystose (19 g/L), 6-kestose (12 g/L), neokestose (10 g/L) and a disaccharide (34 g/L) that after its purification and NMR analysis was identified as blastose [Fru-β(2 → 6)-Glc]. P. sizovae was very selective for the formation of 1F-FOS (in particular 1-kestose) with minor contribution of neoFOS and negligible of levan-type FOS.
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biochemical characterization of a beta fructofuranosidase from rhodotorula dairenensis with transfructosylating activity
Fems Yeast Research, 2009Co-Authors: Patricia Gutierrezalonso, Lucia Fernandezarrojo, Francisco J. Plou, Maria FernandezlobatoAbstract:: An extracellular beta-fructofuranosidase from the yeast Rhodotorula dairenensis was characterized biochemically. The enzyme molecular mass was estimated to be 680 kDa by analytical gel filtration and 172 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, of which the N-linked carbohydrate accounts for 16% of the total mass. It displays optimum activity at pH 5 and 55-60 degrees C. The enzyme shows broad substrate specificity, hydrolyzing sucrose, 1-kestose, Nystose, leucrose, raffinose and inulin. Although the main reaction catalyzed by this enzyme is sucrose hydrolysis, it also exhibits transfructosylating activity that, unlike other microbial beta-fructofuranosidases, produces a varied type of prebiotic fructooligosaccharides containing beta-(2-->1)- and beta-(2-->6)-linked fructose oligomers. The maximum concentration of fructooligosaccharides was reached at 75% sucrose conversion and it was 87.9 g L(-1). The 17.0% (w/w) referred to the total amount of sugars in the reaction mixture. At this point, the amounts of 6-kestose, neokestose, 1-kestose and tetrasaccharides were 68.9, 10.6, 2.6 and 12.7 g L(-1), respectively.
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immobilisation of fructosyltransferase from aspergillus aculeatus on epoxy activated sepabeads ec for the synthesis of fructo oligosaccharides
Journal of Molecular Catalysis B-enzymatic, 2005Co-Authors: Iraj Ghazi, Lucia Fernandezarrojo, Francisco J. Plou, Aranzazu Gomez De Segura, Miguel Alcalde, M Yates, Luisa M Rojascervantes, Antonio BallesterosAbstract:Abstract The transfructosylating activity present in two commercial pectinase preparations (Pectinex Ultra SP-L, from Aspergillus aculeatus, and Rapidase TF, from Aspergillus niger) was studied. Pectinex Ultra SP-L, which has a high transferase/hydrolase ratio, was covalently immobilised on a polymethacrylate-based polymer (Sepabeads® EC) activated with epoxy groups. The influence of pore volume and average pore size on biocatalyst performance was studied for two of these carriers (Sepabeads EC-EP3 and EC-EP5). Several parameters that affect immobilisation, such as buffer concentration, pH and amount (mg) of protein added per gram of support (varied over the range 30:1–200:1), were analysed. We found that Pectinex Ultra SP-L can be efficiently immobilised on these supports without adding any external salt or buffer. Using Sepabeads EC-EP5 – whose pore volume (1.67 cm3/g) and pore size (800 nm) are higher than those corresponding to Sepabeads EC-EP3 – the activity towards sucrose reached 25.9 U/g biocatalyst. The immobilised fructosyltransferase was applied to the batch synthesis of fructo-oligosaccharides (FOS) using 630 g/l sucrose to shift activity towards transfructosylation in detriment of hydrolysis. The FOS concentration reached a maximum value of 387 g/l after 36 h (240 g/l 1-kestose, 144 g/l Nystose and 3 g/l 1F-fructofuranosyl-Nystose), which corresponds to 61.5% (w/w) of the total carbohydrates in the mixture. The features of these immobilised biocatalysts are very attractive for their application in batch and fixed-bed bioreactors.
Norio Shiomi - One of the best experts on this subject based on the ideXlab platform.
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Chemical Structure and Localization of Levan, the Predominant Fructan Type in Underground Systems of Gomphrena marginata (Amaranthaceae)
Frontiers Media S.A., 2018Co-Authors: Emanuela O. Joaquim, Adriana H. Hayashi, Luce M. B. Torres, Rita C. L. Figueiredo-ribeiro, Norio Shiomi, Fernanda S. De Sousa, João H. G. Lago, Maria A. M. CarvalhoAbstract:Gomphrena marginata Seub. (Amaranthaceae) is an endemic species from Brazilian campos rupestres with a fructan accumulating underground reserve system. Analyses of high performance anion exchange chromatography (HPAEC–PAD) revealed the presence of the soluble carbohydrates glucose, fructose, sucrose, 1-kestose, 6-kestose, Nystose and fructans with degree of polymerization (DP) up to approximately 40 fructose units. Data of 1H and 13C Nuclear Magnetic Resonance (NMR) spectroscopy, including Heteronuclear Single-Quantum Correlation (HSQC) and Heteronuclear Multiple-Bonds Correlation (HMBC) showed the presence of β (2,6) linkages, characteristic of the linear molecule of levan-type fructan(2,6). These results confirmed previous studies suggesting that the reserve carbohydrate in the underground system of this species was levan-type fructans, similar to that of G. macrocephala. Structural analyses of the thickened underground system using light microscopy revealed a mixed origin system consisting mainly of a gemmiferous tuberous root with the upper region formed by short branched stems, both presenting vascular cylinders with unusual growth patterns. Fructan spherocrystals were visualized under polarized light and scanning electron microscopy (SEM) mostly in the cortex and vascular cylinder in both thickened stem and root. In addition to data reported in the literature concerning the occurrence of fructans in the Amaranthaceae, the results presented here suggest that fructans are a trait in this family while the levan-type fructan prevail in Gomphrena species
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Image_1_Chemical Structure and Localization of Levan, the Predominant Fructan Type in Underground Systems of Gomphrena marginata (Amaranthaceae).pdf
2018Co-Authors: Emanuela O. Joaquim, Adriana H. Hayashi, Luce M. B. Torres, Rita C. L. Figueiredo-ribeiro, Norio Shiomi, Fernanda S. De Sousa, João H. G. Lago, Maria A. M. CarvalhoAbstract:Gomphrena marginata Seub. (Amaranthaceae) is an endemic species from Brazilian campos rupestres with a fructan accumulating underground reserve system. Analyses of high performance anion exchange chromatography (HPAEC–PAD) revealed the presence of the soluble carbohydrates glucose, fructose, sucrose, 1-kestose, 6-kestose, Nystose and fructans with degree of polymerization (DP) up to approximately 40 fructose units. Data of 1H and 13C Nuclear Magnetic Resonance (NMR) spectroscopy, including Heteronuclear Single-Quantum Correlation (HSQC) and Heteronuclear Multiple-Bonds Correlation (HMBC) showed the presence of β (2,6) linkages, characteristic of the linear molecule of levan-type fructan(2,6). These results confirmed previous studies suggesting that the reserve carbohydrate in the underground system of this species was levan-type fructans, similar to that of G. macrocephala. Structural analyses of the thickened underground system using light microscopy revealed a mixed origin system consisting mainly of a gemmiferous tuberous root with the upper region formed by short branched stems, both presenting vascular cylinders with unusual growth patterns. Fructan spherocrystals were visualized under polarized light and scanning electron microscopy (SEM) mostly in the cortex and vascular cylinder in both thickened stem and root. In addition to data reported in the literature concerning the occurrence of fructans in the Amaranthaceae, the results presented here suggest that fructans are a trait in this family while the levan-type fructan prevail in Gomphrena species.
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variation of 1 kestose Nystose and higher fructooligosaccharides in burdock root stored under different temperature regimes
Agriculture and Biology Journal of North America, 2010Co-Authors: Yojiro Ishiguro, Noureddine Benkeblia, Shuichi Onodera, Taiki Yoshihira, Shinichi Kosaka, Norio ShiomiAbstract:The variation of fructooligosccharides (FOSs) of differed degree of polymerization (DP), 1kestose, Nystose, GF4, GF5, GF6, GF7 and GF8 in burdock roots stored during 42 days under three different temperature regimes, 0, 15 and 20 °C was investigated. Results showed that 1kestose increased more significantly at 0 and 15 °C, and Nystose showed similar variation but the increase observed at 0 °C was higher than those observed at 15 and 20 °C. The fructosylNystose (GF4) increased significantly during the first three days of storage but slightly and progressively during the four following weeks, and the GF5 and GF6 showed close pattern under the three different storage temperatures. The higher oligomer GF7 showed similar variation to these of GF6 but the increase during the first four weeks of storage was lower at 0 °C. At 0 °C, GF8 showed similar variation to those of GF5, GF6 and GF7, but at 15 and 20 °C, GF8 increased after three days and remained quite stable during three weeks but increased at 20 °C during the last two weeks. Overall, results showed that the different FOS increased significantly during the first four weeks at 15 and 20 °C and then decreased, while this decrease was not observed at 0 °C.
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synthesis and structural analysis of five novel oligosaccharides prepared by glucosyltransfer from β d glucose 1 phosphate to isokestose and Nystose using thermoanaerobacter brockii kojibiose phosphorylase
Carbohydrate Research, 2003Co-Authors: Hideki Okada, Eri Fukushi, Shuichi Onodera, Tomoyuki Nishimoto, Jun Kawabata, Masanori Kikuchi, Norio ShiomiAbstract:Abstract Five novel oligosaccharides (tetra-, penta- and hexa-saccharides) were synthesized by glucosyltransfer from β- d -glucose 1-phosphate to isokestose ( O -β- d -fructofuranosyl-(2→1)- O -β- d -fructofuranosyl-(2→1)-α- d -glucopyranoside) or Nystose ( O -β- d -fructofuranosyl-(2→1)- O -β- d -fructofuranosyl-(2→1)- O -β- d -fructofuranosyl-(2→1)-α- d -glucopyranoside) using Thermoanaerobacter brockii kojibiose phosphorylase. The oligosaccharides were identified as 2(2-α- d -glucopyranosyl) m isokestose; [ O -α- d -glucopyranosyl-(1→2)] m - O -[β- d -fructofuranosyl-(2→1)] 2 -α- d -glucopyranoside: m =1, 2, and 3, and 2(2-α- d -glucopyranosyl) n Nystose; [ O -α- d -glucopyranosyl-(1→2)] n - O -[β- d -fructofuranosyl-(2→1)] 3 -α- d -glucopyranoside: n =1 and 2 using gas liquid chromatography analysis of the methyl derivatives, and MALDI-TOF-MS and NMR measurements of the newly formed oligosaccharides. 1 H, 13 C NMR signals of each saccharide were assigned using 2D-NMR techniques, including COSY, HSQC, HSQC–TOCSY, HMBC, CH 2 -selected E-HSQC, and CH 2 -selected E-HSQC–TOCSY.
H H Jonker - One of the best experts on this subject based on the ideXlab platform.
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purification and characterization of the enzymes of fructan biosynthesis in tubers of helianthus tuberosus colombia ii purification of sucrose sucrose 1 fructosyltransferase and reconstitution of fructan synthesis in vitro with purified sucrose sucro
Plant Physiology, 1996Co-Authors: A J Koops, H H JonkerAbstract:Sucrose:sucrose 1-fructosyltransferase (1-SST), an enzyme involved in fructan biosynthesis, was purified to homogeneity from tubers of Helianthus tuberosus that were harvested in the accumulation phase. Gel filtration under native conditions predicted a molecular mass of about 67 kD. Electrophoresis or gel filtration under denaturing conditions yielded a 27- and a 55-kD fragment. 1-SST preferentially catalyzed the conversion of sucrose into the trisaccharide 1-kestose (GF2). Other reactions catalyzed by 1-SST at a lower rate were self-transfructosylations with GF2 and 1,1-Nystose (GF3) as substrates yielding GF3 and 1,1,1-fructosylNystose, respectively, as products. 1-SST also catalyzed the removal of the terminal fructosyl unit from both GF2 and GF3, which resulted in the release of sucrose and GF2, respectively, and free Fru. The purified enzyme did not display [beta]-fructosidase activity. An enzyme mixture of purified 1-SST and fructan:fructan 1-fructosyltransferase, both isolated from tubers, was able to synthesize fructans up to a degree of polymerization of at least 13 with sucrose as a sole substrate.
Debin Huang - One of the best experts on this subject based on the ideXlab platform.
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the tetrasaccharide Nystose trihydrate crystal structure analysis and hydrogen bonding
Carbohydrate Research, 1993Co-Authors: George A Jeffrey, Debin HuangAbstract:Abstract The crystal structure of Nystose trihydate, O -β- d -fructofuranosyl-(2 → 1)- O -β- d -fructofuranosyl-(2 → 1)-β- d -fructofuranosyl α- d -glucopyranoside trihydate, C 24 H 42 O 21 ·3H 2 O, has been determined using Cu K α X-ray data at 121 K. The space group is P 2 1 2 1 2 1 , with 4 molecules in a unit cell of dimensions a = 10.155(1), b = 13,506(1), and c = 23.278(2) A. The structure was refined to R = 0.052 and R w = 0.048 for 2734 observed structure amplitudes. The α- d -glucopyranose unit of the molecule has the normal 4 C 1 chair conformation and the three fructofuranose units have twist conformations lying between E 3 and 4 T 5 . One of the three water molecules is distributed over two sites: W-3 with occupancy 0.80 and W-3′ with occupancy 0.20. All the hydrogen atoms were located on the difference synthesis with the exception of those attached to the low-occupancy water site. All hydroxyls, two of the three linkage oxygen atoms, and the water molecules are involved in a complex three-dimensional network which can be decomposed into a series of infinite chains intersecting at the water molecules to form homo- and hetero-dromic cycles.