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Jianping Wang - One of the best experts on this subject based on the ideXlab platform.
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genome wide association studies identified resistance loci to orange rust and yellow leaf virus diseases in Sugarcane saccharum spp
2019Co-Authors: Xiping Yang, Sushma Sood, Ziliang Luo, James Todd, Jianping WangAbstract:Sugarcane (Saccharum spp.) supplies globally ∼80% of table sugar and 60% of bioethanol. Sugarcane orange rust and Sugarcane yellow leaf virus (SCYLV) are major Sugarcane diseases, causing up to 50 and 40% yield losses, respectively. Sugarcane cultivars resistant to these diseases are needed to sustain Sugarcane production in several regions. Dissecting DNA sequence variants controlling disease resistance provides a valuable tool for fulfilling a breeding strategy to develop resistant cultivars. In this study, we evaluated disease reactions to orange rust and SCYLV of a Sugarcane diversity panel in repeated trials. We conducted a genome-wide association study between high-density markers and disease resistance reactions. We identified 91 putative DNA markers and 82 candidate genes significantly associated with resistance to one of the two diseases. These provide an important genetic resource for finding genes and molecular markers for disease resistance. Our results emphasized the importance of utilizing a wide germplasm collection for breeding resistant Sugarcane cultivars.
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identifying quantitative trait loci qtls and developing diagnostic markers linked to orange rust resistance in Sugarcane saccharum spp
2018Co-Authors: Xiping Yang, Sushma Sood, Jianping Wang, Erik Hanson, Md Sariful Islam, Stephanie Maya, J C ComstockAbstract:Sugarcane (Saccharum spp.) is an important economic crop, contributing up to 80% of table sugar used in the world and has become a promising feedstock for biofuel production. Sugarcane production has been threatened by many diseases, and fungicide applications for disease control have been opted out for sustainable agriculture. Orange rust is one of the major diseases impacting Sugarcane production worldwide. Identifying quantitative trait loci (QTLs) and developing diagnostic markers are valuable for breeding programs to expedite release of superior Sugarcane cultivars for disease control. In this study, an F1 segregating population derived from a cross between two hybrid Sugarcane clones, CP95-1039 and CP88-1762, was evaluated for orange rust resistance in replicated trails. Three QTLs controlling orange rust resistance in Sugarcane (qORR109, qORR4 and qORR102) were identified for the first time ever, which can explain 58, 12 and 8% of the phenotypic variation, separately. We also characterized 1,574 Sugarcane putative resistance (R) genes. These Sugarcane putative R genes and simple sequence repeats in the QTL intervals were further used to develop diagnostic markers for marker-assisted selection of orange rust resistance. A PCR-based Resistance gene-derived maker, G1 was developed, which showed significant association with orange rust resistance. The putative QTLs and marker developed in this study can be effectively utilized in Sugarcane breeding programs to facilitate the selection process, thus contributing to the sustainable agriculture for orange rust disease control.
Rott Philippe - One of the best experts on this subject based on the ideXlab platform.
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Comparison of the Virome of Quarantined Sugarcane Varieties and the Virome of Grasses Growing near the Quarantine Station
2021Co-Authors: Daugrois Jean, Fernandez Emmanuel, Filloux Denis, Rott Philippe, Julian Charlotte, Claude Lisa, Ferdinand Romain, Fontes Hugo, Roumagnac PhilippeAbstract:International audienceVisacane is a Sugarcane quarantine station located in the South of France, far away from Sugarcane growing areas. Visacane imports up to 100 Sugarcane varieties per year, using safe control and confinement measures of plants and their wastes to prevent any risk of pathogen spread outside of the facilities. Viruses hosted by the imported material are either known or unknown to cause disease in cultivated Sugarcane. Poaceae viruses occurring in plants surrounding the quarantine glasshouse are currently unknown. These viruses could be considered as a source of new Sugarcane infections and potentially cause new Sugarcane diseases in cases of confinement barrier failure. The aim of this study was to compare the plant virome inside and outside of the quarantine station to identify potential confinement failures and risks of cross infections. Leaves from quarantined Sugarcane varieties and from wild Poaceae growing near the quarantine were collected and processed by a metagenomics approach based on virion-associated nucleic acids extraction and library preparation for Illumina sequencing. While viruses belonging to the same virus genus or family were identified in the Sugarcane quarantine and its surroundings, no virus species was detected in both environments. Based on the data obtained in this study, no virus movement between quarantined Sugarcane and nearby grassland has occurred so far, and the confinement procedures of Visacane appear to be properly implemented
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Lack of transmission of Sugarcane yellow leaf virus in Florida from Columbus grass and Sugarcane to Sugarcane with aphids or mites
2020Co-Authors: Boukari Wardatou, Hincapie Martha, Sood Sushma, Wei Chunyan, Tang Lihua, Naranjo Moramay, Nuessly Gregg, Beuzelin, Julien M., Rott PhilippeAbstract:Sugarcane yellow leaf virus (SCYLV), the causal agent of yellow leaf disease, naturally infects at least three plant species in Florida: Sugarcane (Saccharum spp.), the weed Columbus grass (Sorghum almum) and cultivated sorghum (S. bicolor). All three hosts are also colonized by the Sugarcane aphid (Melanaphis sacchari), the main vector of SCYLV worldwide. To understand the high incidence of SCYLV observed in Sugarcane commercial fields and in germplasm collections, we investigated the transmission efficiency of SCYLV from Sugarcane and Columbus grass to Sugarcane using the Sugarcane aphid and a spider mite (Oligonychus grypus) that also tested positive for SCYLV in Florida. Healthy and SCYLV-infected leaf pieces of Sugarcane and Columbus grass carrying viruliferous aphids or spider mites were transferred to virus-free plants of the yellow leaf susceptible Sugarcane cultivar CP96-1252. Three- and 6-months post inoculation, the 108 aphid-inoculated plants of Columbus grass and the 90 mite-inoculated plants of Sugarcane tested negative for SCYLV by tissue blot immunoassay (TBIA) or reverse transcription polymerase chain reaction (RT-PCR). Similar results were obtained for 162 aphid-inoculated plants of Sugarcane, except for two plants that tested positive for SCYLV by TBIA and RT-PCR. In two field experiments planted with SCYLV-free and virus-infected Sugarcane (cultivar CP96-1252), only 18–28% of healthy plants became infected during a 24- to 28-month period. SCYLV prevalence in these field experiments did not differ between aphicide treated and untreated plots. Incidence of M. sacchari haplotypes in the Everglades agricultural area also indicated that the predominant haplotype that is currently colonizing Sugarcane was not a vector of SCYLV in Florida. Lack of virus transmission by the spider mite suggested that this arthropod only acquired the virus when feeding on infected plants but was unable to transmit SCYLV. The current vector of SCYLV in Florida remains to be identified
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Sugarcane mosaic and sorghum mosaic are caused by two different strains of Sugarcane mosaic virus in the Everglades Agricultural Area
2019Co-Authors: Hincapie Martha, Sood Sushma, Mollov, Dimitre S., Rott PhilippeAbstract:Sugarcane mosaic virus (SCMV) infects Sugarcane and other species in the family Poaceae. In the Everglades Agricultural Area (EAA) of south Florida, we detected SCMV in Columbus grass (Sorghum almum), maize (Zea mays), sorghum (Sorghum bicolor), St. Augustine grass (Stenotaphrum secundatum), southern crabgrass (Digitaria ciliaris), and Sugarcane (Saccharum spp.). The entire coat protein (CP) of 43 SCMV isolates from these six host plants was amplified by reverse-transcription polymerase chain reaction and sequenced. The size of the CP sequences ranged from 837 to 1008 nucleotides. A phylogenetic analysis was performed using the sequences obtained in this study along with the CP sequences of other SCMV isolates retrieved from GenBank. SCMV isolates causing mosaic of Sugarcane formed a distinct phylogenetic group. This group was closest to a separate phylogenetic group formed by SCMV isolates from Florida causing mosaic of Columbus grass, maize, and sorghum. Maize and sorghum showed mosaic symptoms when mechanically inoculated with SCMV isolates from Sugarcane and Columbus grass. SCMV was not transmitted mechanically from Sugarcane to Columbus grass or from Columbus grass to Sugarcane. This suggests that SCMV from Sugarcane and SCMV from Columbus grass are two different strains of the virus, and that Columbus grass is not the inoculum source for SCMV infecting Sugarcane in the EAA. Furthermore, close phylogenetic relationship between isolates of SCMV from Columbus grass, maize, and sorghum suggests that the same virus strain is naturally spread among these plants in south Florida
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Evidence of host plant specialization among the U.S. Sugarcane aphid (Hemiptera: Aphididae) genotypes
2019Co-Authors: Paudyal Sulochana, Rott Philippe, Armstrong, John Scott, Harris-shultz Karen, Wang Hongliang, Giles Kristopher, Payton MarkAbstract:The Sugarcane aphid (Melanaphis sacchari (Zehnter) (Hemiptera: Aphididae)) has become a serious pest of sorghum (Sorghum bicolor (L.) Moench) in the United States since it was detected in 2013. The Sugarcane aphid was considered only a pest of Sugarcane in Florida and Louisiana for over three decades before the 2013 outbreak. Recent studies suggest that the 2013 outbreak in sorghum was due to the introduction of a new genotype. Our scope for this study was to quantify phenotypic behaviors (host suitability as measured through life table statistics) and genetic diversity among Sugarcane aphid clones collected from different hosts. We collected Sugarcane aphid clones from sorghum (SoSCA), Sugarcane (SuSCA), and Columbus grass (CoSCA) and determined biodemographic data and host suitability when offered five different hosts plants including, Sugarcane, Columbus grass, Johnsongrass, and a resistant and susceptible grain sorghum. Sugarcane aphid clones collected from different hosts varied in performance among hosts plants. The survivorship and reproduction of the Sugarcane-collected aphid clone (SuSCA) was significantly higher when offered Sugarcane (>85%) as compared to other hosts and in contrast, there was negligible survival and reproduction when SoSCA and CoSCA were offered Sugarcane as host. Genotyping of the aphid clones collected from various hosts with microsatellite markers indicated that SuSCA was a different genotype and belonged to the multilocus lineage MLL-D as compared to SoSCA and CoSCA which belonged to MLL-F. Our results suggest there exists two different host-specific biotypes of the Sugarcane aphid within the United States
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Evidence of host plant specialization among the U.S. Sugarcane aphid(Hemiptera: Aphididae) genotypes
2019Co-Authors: Paudyal Sulochana, Rott Philippe, Armstrong, John Scott, Harris-shultz Karen, Wang Hongliang, Giles Kristopher, Payton MarkAbstract:The Sugarcane aphid (Melanaphis sacchari (Zehnter) (Hemiptera: Aphididae)) has become a serious pest of sorghum (Sorghum bicolor (L.) Moench) in the U.S. since it was detected in Texas in 2013. The Sugarcane aphid was considered only a pest on Sugarcane in Florida and Louisiana for over three decades before the 2013 outbreak. Recent studies suggest that the 2013 outbreak in sorghum was due to the introduction of new genotype. Our scope for this study was to quantify the phenotypic behaviors (host suitability as measured through life table statistics) and genetic diversity among Sugarcane aphid clones collected from different hosts. We collected a diverse group of Sugarcane aphid clones from sorghum (SoSCA), Sugarcane (SuSCA), and Columbus grass (CoSCA) and determined host suitability when introduced to five different plants including Sugarcane, Columbus grass, Johnsongrass, and a resistant and susceptible grain sorghum. Sugarcane aphid clones from different hosts and geographical regions varied in performance among plant hosts. The survivorship and reproduction of Sugarcane collected aphid clone (SuSCA) was significantly higher when offered Sugarcane (>85%) as compared to other hosts and in contrast, there was negligible survival and reproduction when SoSCA and CoSCA were offered Sugarcane as host. Genotyping of the aphid clones collected from various hosts with microsatellite markers indicated that SuSCA was a different genotype and belonged to multilocus lineage, MLL-D as compared to SoSCA and CoSCA which belonged to MLL-F. Our results suggest that there exist two different biotypes of M. sacchari within the U.S., and that they cannot be distinguished by taxonomic or morphometric characteristics
Xiping Yang - One of the best experts on this subject based on the ideXlab platform.
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genome wide association studies identified resistance loci to orange rust and yellow leaf virus diseases in Sugarcane saccharum spp
2019Co-Authors: Xiping Yang, Sushma Sood, Ziliang Luo, James Todd, Jianping WangAbstract:Sugarcane (Saccharum spp.) supplies globally ∼80% of table sugar and 60% of bioethanol. Sugarcane orange rust and Sugarcane yellow leaf virus (SCYLV) are major Sugarcane diseases, causing up to 50 and 40% yield losses, respectively. Sugarcane cultivars resistant to these diseases are needed to sustain Sugarcane production in several regions. Dissecting DNA sequence variants controlling disease resistance provides a valuable tool for fulfilling a breeding strategy to develop resistant cultivars. In this study, we evaluated disease reactions to orange rust and SCYLV of a Sugarcane diversity panel in repeated trials. We conducted a genome-wide association study between high-density markers and disease resistance reactions. We identified 91 putative DNA markers and 82 candidate genes significantly associated with resistance to one of the two diseases. These provide an important genetic resource for finding genes and molecular markers for disease resistance. Our results emphasized the importance of utilizing a wide germplasm collection for breeding resistant Sugarcane cultivars.
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target enrichment sequencing of 307 germplasm accessions identified ancestry of ancient and modern hybrids and signatures of adaptation and selection in Sugarcane saccharum spp a sweet crop with bitter genomes
2019Co-Authors: Xiping Yang, Ziliang Luo, James Todd, Jian Song, Ze Peng, Dev Paudel, Qian You, Erik Hanson, Zifan ZhaoAbstract:Sugarcane (Saccharum spp.) is a highly energy‐efficient crop primarily for sugar and bio‐ethanol production. Sugarcane genetics and cultivar improvement have been extremely challenging largely due to its complex genomes with high polyploidy levels. In this study, we deeply sequenced the coding regions of 307 Sugarcane germplasm accessions. Nearly five million sequence variations were catalogued. The average of 98× sequence depth enabled different allele dosages of sequence variation to be differentiated in this polyploid collection. With selected high‐quality genome‐wide SNPs, we performed population genomic studies and environmental association analysis. Results illustrated that the ancient Sugarcane hybrids, S. barberi and S. sinense, and modern Sugarcane hybrids are significantly different in terms of genomic compositions, hybridization processes and their potential ancestry contributors. Linkage disequilibrium (LD) analysis showed a large extent of LD in Sugarcane, with 962.4 Kbp, 2739.2 Kbp and 3573.6 Kbp for S. spontaneum, S. officinarum and modern S. hybrids respectively. Candidate selective sweep regions and genes were identified during domestication and historical selection processes of Sugarcane in addition to genes associated with environmental variables at the original locations of the collection. This research provided an extensive amount of genomic resources for Sugarcane community and the in‐depth population genomic analyses shed light on the breeding and evolution history of Sugarcane, a highly polyploid species.
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identifying quantitative trait loci qtls and developing diagnostic markers linked to orange rust resistance in Sugarcane saccharum spp
2018Co-Authors: Xiping Yang, Sushma Sood, Jianping Wang, Erik Hanson, Md Sariful Islam, Stephanie Maya, J C ComstockAbstract:Sugarcane (Saccharum spp.) is an important economic crop, contributing up to 80% of table sugar used in the world and has become a promising feedstock for biofuel production. Sugarcane production has been threatened by many diseases, and fungicide applications for disease control have been opted out for sustainable agriculture. Orange rust is one of the major diseases impacting Sugarcane production worldwide. Identifying quantitative trait loci (QTLs) and developing diagnostic markers are valuable for breeding programs to expedite release of superior Sugarcane cultivars for disease control. In this study, an F1 segregating population derived from a cross between two hybrid Sugarcane clones, CP95-1039 and CP88-1762, was evaluated for orange rust resistance in replicated trails. Three QTLs controlling orange rust resistance in Sugarcane (qORR109, qORR4 and qORR102) were identified for the first time ever, which can explain 58, 12 and 8% of the phenotypic variation, separately. We also characterized 1,574 Sugarcane putative resistance (R) genes. These Sugarcane putative R genes and simple sequence repeats in the QTL intervals were further used to develop diagnostic markers for marker-assisted selection of orange rust resistance. A PCR-based Resistance gene-derived maker, G1 was developed, which showed significant association with orange rust resistance. The putative QTLs and marker developed in this study can be effectively utilized in Sugarcane breeding programs to facilitate the selection process, thus contributing to the sustainable agriculture for orange rust disease control.
R E F Munhoz - One of the best experts on this subject based on the ideXlab platform.
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avaliacao de clones de cana de acucar promissores rbs quanto a resistencia a broca da cana diatraea saccharalis na regiao noroeste do parana
2008Co-Authors: Paulo Afonso Demetrio, Patricia Da Costa Zonetti, R E F MunhozAbstract:Sugarcane (Saccharum ssp) is a crop generally attacked by the agricultural pest Diatraea saccharalis, known as Sugarcane borer, that besides reducing agricultural production, still cause problems of fermentation in the alcohol and sugar’s mill. The fast expansion of the agricultural yield put on decline some cultivars, reducing the varietal options regarding the resistance to the Sugarcane borer. The present work had as objective to select the RBs clones of the series 97 coming from hybridization carried out in 1997 at Serra do Ouro, in Murici – Alagoas, by the Breeding Sugarcane/ridesa program, with better resistance to the Diatraea saccharalis. This research was done comparing RB’s Sugarcane clones series 97, relating to the Sugarcane infestation index and relating to the industrial yield of these clones, through a experiment carried out in Alto Alegre’s mill, on Colorado City, in the Northwest of Parana State. The clones RB 975311 and RB 9755286 showed a good resistance to the Sugarcane borer infestation, an average of 1,7% e 3,5% respectively, as well showed good pure characteristics exceeding the RB 72454 and RB 835486 standards.
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avaliacao de clones de cana de acucar promissores rb s quanto a resistencia a broca da cana diatraea saccharalis na regiao noroeste do parana
2008Co-Authors: Paulo Afonso Demetrio, Patricia Da Costa Zonetti, R E F MunhozAbstract:Sugarcane (Saccharum ssp) is a crop generally attacked by the agricultural pest Diatraea saccharalis, known as Sugarcane borer, that besides reducing agricultural production, still cause problems of fermentation in the alcohol and sugar’s mill. The fast expansion of the agricultural yield put on decline some cultivars, reducing the varietal options regarding the resistance to the Sugarcane borer. The present work had as objective to select the RBs clones of the series 97 coming from hybridization carried out in 1997 at Serra do Ouro, in Murici – Alagoas, by the Breeding Sugarcane/ridesa program, with better resistance to the Diatraea saccharalis. This research was done comparing RB’s Sugarcane clones series 97, relating to the Sugarcane infestation index and relating to the industrial yield of these clones, through a experiment carried out in Alto Alegre’s mill, on Colorado City, in the Northwest of Parana State. The clones RB 975311 and RB 9755286 showed a good resistance to the Sugarcane borer infestation, an average of 1,7% e 3,5% respectively, as well showed good pure characteristics exceeding the RB 72454 and RB 835486 standards.
Youxiong Que - One of the best experts on this subject based on the ideXlab platform.
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transcriptional analysis identifies major pathways as response components to sporisorium scitamineum stress in Sugarcane
2018Co-Authors: Ning Huang, Hui Ling, Feng Liu, Jinlong Guo, Shiwu Gao, Youxiong QueAbstract:Abstract Background Sugarcane smut, which is caused by Sporisorium scitamineum, is a severe fungal disease affecting Sugarcane. However, the major pathways involved in the interaction between Sugarcane and S. scitamineum remains unclear. Results In the present study, suppression subtractive hybridization (SSH) library construction, together with reverse northern blotting, was conducted on the most prevalent Sugarcane genotype ROC22 challenged with S. scitamineum. After alignment and homologous expressed sequence tag (EST) assembly, a total of 155 differentially expressed unigenes were identified from SSH libraries. Totally, 26 of 155 differentially expressed unigenes were analyzed by qRT-PCR in Sugarcane smut-resistant genotype YC05-179 and susceptible genotype ROC22. Genes encoded two unknown protein (Q1 and Q11), serine/threonine kinase (Q2), fiber protein (Q3), eukaryotic translation initiation factor 5A (Q23), and Sc14-3-3-like protein (Q24) were induced in Sugarcane smut-resistant genotype YC05-179 but inhibited in susceptible genotype ROC22. Based on the differential expression data achieved from SSH libraries and qRT-PCR, we found that, serine/threonine kinases, Ca2+ sensors, mitogen-activated protein genes and some NBS-LRR genes may involve in the signal recognition and transduction of smut fungus infection in Sugarcane. While in the plant hormone signaling pathways, the genes related to auxin, abscisic acid, salicylic acid and ethylene were more apparently in response to smut fungus invasion. The hypersensitive response, protein metabolism, polyamine synthesis, and cell wall formation may play an important role in Sugarcane defense against smut fungus colonization. Additionally, the Sc14-3-3 might serve as a molecular modulator in Sugarcane being immune to smut disease by interacting with proteins like ScGAPN (Q10), which have been further verified by BiFC assay. Conclusions The findings of the present study could provide a general view about gene pathways involving in Sugarcane defense against smut disease and facilitate a better understanding of the molecular mechanism underlying Sugarcane-S. scitamineum interaction.
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identification and evaluation of pcr reference genes for host and pathogen in Sugarcane sporisorium scitamineum interaction system
2018Co-Authors: Ning Huang, Hui Ling, Feng Liu, Huaying Mao, Xu Zhang, Ling Wang, Rukai Chen, Youxiong QueAbstract:Sugarcane (Saccharum L. plant) is an important crop for sugar and bio-energy production around the world. Among Sugarcane diseases, smut caused by Sporisorium scitamineum is one of the major fungal diseases causing severe losses to the Sugarcane industry. The use of PCR reference genes is essential to the normalization of data on gene expression involving the Sugarcane-S. scitamineum interaction system; however, no report that addresses criteria in selecting these reference genes has been published to date. In this study, 10 Sugarcane genes and eight S. scitamineum genes were selected as candidate PCR reference genes in the Sugarcane-S. scitamineum interaction system. The stability and reliability of these 18 candidate genes were analyzed in smut-resistant (NCo376) and -susceptible (YC71–374) genotypes using the statistical algorithms geNorm, NormFinder, BestKeeper, and deltaCt method. Subsequently, the relative expression levels of the Sugarcane chitinase I-3 gene and S. scitamineum chorismate mutase gene were determined to validate the applicability of these Sugarcane and S. scitamineum PCR reference genes, respectively. We finally found that the acyl-CoA dehydrogenase gene (ACAD), serine/arginine repetitive matrix protein 1 gene (SARMp1), or their combination (ACAD + SARMp1) could be utilized as the most suitable reference genes for normalization of Sugarcane gene expression in Sugarcane bud tissues after S. scitamineum infection. Similarly, the inosine 5′-monophosphate dehydrogenase gene (S10), the SEC65-signal recognition particle subunit gene (S11), or their combination (S10 + S11) were suitable for normalization of S. scitamineum gene expression in Sugarcane bud tissues. The PCR reference genes ACAD, SARMp1, S10, and S11 may be employed in gene transcriptional studies involving the Sugarcane-S. scitamineum interaction system.
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Identification and evaluation of PCR reference genes for host and pathogen in Sugarcane-Sporisorium scitamineum interaction system
2018Co-Authors: Ning Huang, Hui Ling, Feng Liu, Huaying Mao, Xu Zhang, Ling Wang, Rukai Chen, Youxiong QueAbstract:Abstract Background Sugarcane (Saccharum L. plant) is an important crop for sugar and bio-energy production around the world. Among Sugarcane diseases, smut caused by Sporisorium scitamineum is one of the major fungal diseases causing severe losses to the Sugarcane industry. The use of PCR reference genes is essential to the normalization of data on gene expression involving the Sugarcane-S. scitamineum interaction system; however, no report that addresses criteria in selecting these reference genes has been published to date. Results In this study, 10 Sugarcane genes and eight S. scitamineum genes were selected as candidate PCR reference genes in the Sugarcane-S. scitamineum interaction system. The stability and reliability of these 18 candidate genes were analyzed in smut-resistant (NCo376) and -susceptible (YC71–374) genotypes using the statistical algorithms geNorm, NormFinder, BestKeeper, and deltaCt method. Subsequently, the relative expression levels of the Sugarcane chitinase I-3 gene and S. scitamineum chorismate mutase gene were determined to validate the applicability of these Sugarcane and S. scitamineum PCR reference genes, respectively. We finally found that the acyl-CoA dehydrogenase gene (ACAD), serine/arginine repetitive matrix protein 1 gene (SARMp1), or their combination (ACAD + SARMp1) could be utilized as the most suitable reference genes for normalization of Sugarcane gene expression in Sugarcane bud tissues after S. scitamineum infection. Similarly, the inosine 5′-monophosphate dehydrogenase gene (S10), the SEC65-signal recognition particle subunit gene (S11), or their combination (S10 + S11) were suitable for normalization of S. scitamineum gene expression in Sugarcane bud tissues. Conclusions The PCR reference genes ACAD, SARMp1, S10, and S11 may be employed in gene transcriptional studies involving the Sugarcane-S. scitamineum interaction system
