O-Polysaccharide

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Victor J Morris - One of the best experts on this subject based on the ideXlab platform.

  • characterization of a variant of the polysaccharide acetan produced by a mutant of acetobacter xylinum strain cr1 4
    Journal of Applied Microbiology, 1993
    Co-Authors: C A Maccormick, A. Patrick Gunning, J E Harris, Victor J Morris
    Abstract:

    : Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.

  • Characterization of a variant of the polysaccharide acetan produced by a mutant of Acetobacter xylinum strain CR1/4
    Journal of Applied Microbiology, 1993
    Co-Authors: C A Maccormick, A. Patrick Gunning, J E Harris, Victor J Morris
    Abstract:

    : Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.

C A Maccormick - One of the best experts on this subject based on the ideXlab platform.

  • characterization of a variant of the polysaccharide acetan produced by a mutant of acetobacter xylinum strain cr1 4
    Journal of Applied Microbiology, 1993
    Co-Authors: C A Maccormick, A. Patrick Gunning, J E Harris, Victor J Morris
    Abstract:

    : Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.

  • Characterization of a variant of the polysaccharide acetan produced by a mutant of Acetobacter xylinum strain CR1/4
    Journal of Applied Microbiology, 1993
    Co-Authors: C A Maccormick, A. Patrick Gunning, J E Harris, Victor J Morris
    Abstract:

    : Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.

J E Harris - One of the best experts on this subject based on the ideXlab platform.

  • characterization of a variant of the polysaccharide acetan produced by a mutant of acetobacter xylinum strain cr1 4
    Journal of Applied Microbiology, 1993
    Co-Authors: C A Maccormick, A. Patrick Gunning, J E Harris, Victor J Morris
    Abstract:

    : Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.

  • Characterization of a variant of the polysaccharide acetan produced by a mutant of Acetobacter xylinum strain CR1/4
    Journal of Applied Microbiology, 1993
    Co-Authors: C A Maccormick, A. Patrick Gunning, J E Harris, Victor J Morris
    Abstract:

    : Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.

A. Patrick Gunning - One of the best experts on this subject based on the ideXlab platform.

  • characterization of a variant of the polysaccharide acetan produced by a mutant of acetobacter xylinum strain cr1 4
    Journal of Applied Microbiology, 1993
    Co-Authors: C A Maccormick, A. Patrick Gunning, J E Harris, Victor J Morris
    Abstract:

    : Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.

  • Characterization of a variant of the polysaccharide acetan produced by a mutant of Acetobacter xylinum strain CR1/4
    Journal of Applied Microbiology, 1993
    Co-Authors: C A Maccormick, A. Patrick Gunning, J E Harris, Victor J Morris
    Abstract:

    : Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.

Chul Soo Shin - One of the best experts on this subject based on the ideXlab platform.

  • immuno stimulating effect of the endo polysaccharide produced by submerged culture of inonotus obliquus
    Life Sciences, 2005
    Co-Authors: Yeo Dae Yoon, Joon Ki Jung, Chul Soo Shin
    Abstract:

    Abstract Inonotus obliquus BELYU1102 was selected from 12 different strains of Inonotus as a producer of immuno-stimulating polysaccharide. After a batch fermentation of I. obliquus BELYU1102 was carried out in a 300 l pilot vessel, endO-Polysaccharide and exO-Polysaccharide were both obtained. The proliferation activity of endO-Polysaccharide for splenic cells was much higher than the activity of exO-Polysaccharide. The active endO-Polysaccharide was produced primarily during the late stationary phase. Enhanced proliferation and polyclonal IgM antibody production were observed in B cells by purified water-soluble endO-Polysaccharide. Nitrite production and expression of IL-1β, IL-6, TNF-α, and iNOS in macrophages were also enhanced. However, the endO-Polysaccharide did not affect the proliferation of T cells, the IL-2 expression of Th1 cells, or the IL-4 expression of Th2 cells. The endO-Polysaccharide showed activities similar to lipopolysaccharide (LPS) for B cells and macrophages, but there was a large difference between the two polysaccharides because cellular activations induced by endO-Polysaccharide were not affected by polymyxin B, a specific inhibitor of LPS. The endO-Polysaccharide appeared to have other cellular binding sites with TLR-4 and did not show a direct toxicity against tumor cells. However, indirect anti-cancer effects via immuno-stimulation were observed. The mycelial endO-Polysaccharide of I. obliquus is a candidate for use as an immune response modifier. Submerged mycelial cultures are advantageous for industrial production of polysaccharides.