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Nick A Van Huizen - One of the best experts on this subject based on the ideXlab platform.

  • human extrahepatic and intrahepatic cholangiocyte organoids show region specific differentiation potential and model cystic fibrosis related bile duct disease
    Scientific Reports, 2020
    Co-Authors: Monique M A Verstegen, Floris J M Roos, Ksenia Burka, Helmuth Gehart, Myrthe Jager, Maaike De Wolf, Marcel J C Bijvelds, Hugo R De Jonge, Arif I Ardisasmita, Nick A Van Huizen
    Abstract:

    The development, homeostasis, and repair of intrahepatic and extrahepatic bile ducts are thought to involve distinct mechanisms including proliferation and maturation of cholangiocyte and progenitor cells. This study aimed to characterize human extrahepatic cholangiocyte organoids (ECO) using canonical Wnt-stimulated culture medium previously developed for intrahepatic cholangiocyte organoids (ICO). Paired ECO and ICO were derived from common bile duct and liver tissue, respectively. Characterization showed both organoid types were highly similar, though some differences in size and gene expression were observed. Both ECO and ICO have cholangiocyte fate differentiation capacity. However, unlike ICO, ECO lack the potential for differentiation towards a hepatocyte-like fate. Importantly, ECO derived from a cystic fibrosis patient showed no CFTR channel activity but normal chloride channel and MDR1 transporter activity. In conclusion, this study shows that ECO and ICO have distinct lineage fate and that ECO provide a competent model to study extrahepatic bile duct diseases like cystic fibrosis.

V Doerges - One of the best experts on this subject based on the ideXlab platform.

  • the reliability of pulse contour derived cardiac output during hemorrhage and after vasopressor administration
    Anesthesia & Analgesia, 2007
    Co-Authors: Berthold Bein, Patrick Meybohm, E Cavus, Jochen Renner, Peter H Tonner, Markus Steinfath, Jens Scholz, V Doerges
    Abstract:

    BACKGROUND: Reliable measurement of cardiac output (CO) is important in the critically ill. Pulse contour-derived CO (PCCO) has been evaluated during stable hemodynamics, but is sensitive to changes in vascular tone and has not been validated under conditions of changing hemodynamics. Furthermore, PCCO requires calibration for the individual vascular impedance by transpulmonary thermodilution CO (TPCO), and the required frequency of recalibration to maintain accurate measurements, especially during changing conditions, has not been confirmed. We compared PCCO measurements of CO with TPCO and continuous and bolus pulmonary artery CO (CCO and BCO, respectively) during conditions of uncontrolled hemorrhage and resuscitation with norepinephrine. METHODS: Thirteen pigs were anesthetized and instrumented for determination of CO by BCO and CCO, respectively, as well as bolus TPCO and PCCO. Uncontrolled hemorrhage was accomplished by liver incision. When mean arterial blood pressure was <25 mm Hg, or heart rate declined progressively to <20% of its peak value, vasopressor therapy was started. TPCO and BCO were performed after induction of anesthesia and 15 min after start of therapy, and PCCO and CCO were obtained repeatedly. CO measurements were compared using Bland-Altman analysis. RESULTS: Mean arterial blood pressure, CO and systemic vascular resistance decreased after hemorrhage (P < 0.001 and <0.01, respectively). Bias and limits of agreement between CCO and PCCO (0.54 L/min; 1.46 L/min) increased after hemorrhage (-3.49; 6.12) and further deteriorated after norepinephrine administration (-8.01; 9.9). After recalibration, bias and limits of agreement returned to -0.51 and 1.28. CONCLUSIONS: PCCO needs frequent recalibration during hemorrhage and after vasopressor administration.

  • the reliability of pulse contour derived cardiac output during hemorrhage and after vasopressor administration
    Anesthesia & Analgesia, 2007
    Co-Authors: Berthold Bein, Patrick Meybohm, E Cavus, Jochen Renner, Peter H Tonner, Markus Steinfath, Jens Scholz, V Doerges
    Abstract:

    BACKGROUND: Reliable measurement of cardiac output (CO) is important in the critically ill. Pulse contour-derived CO (PCCO) has been evaluated during stable hemodynamics, but is sensitive to changes in vascular tone and has not been validated under conditions of changing hemodynamics. Furthermore, PCCO requires calibration for the individual vascular impedance by transpulmonary thermodilution CO (TPCO), and the required frequency of recalibration to maintain accurate measurements, especially during changing conditions, has not been confirmed. We compared PCCO measurements of CO with TPCO and continuous and bolus pulmonary artery CO (CCO and BCO, respectively) during conditions of uncontrolled hemorrhage and resuscitation with norepinephrine. METHODS: Thirteen pigs were anesthetized and instrumented for determination of CO by BCO and CCO, respectively, as well as bolus TPCO and PCCO. Uncontrolled hemorrhage was accomplished by liver incision. When mean arterial blood pressure was 25 mm Hg, or heart rate declined progressively to 20% of its peak value, vasopressor therapy was started. TPCO and BCO were performed after induction of anesthesia and 15 min after start of therapy, and PCCO and CCO were obtained repeatedly. CO measurements were compared using Bland-Altman analysis. RESULTS: Mean arterial blood pressure, CO and systemic vascular resistance decreased after hemorrhage (P 0.001 and 0.01, respectively). Bias and limits of agreement between CCO and PCCO (0.54 L/min; 1.46 L/min) increased after hemorrhage (3.49; 6.12) and further deteriorated after norepinephrine administration (8.01; 9.9). After recalibration, bias and limits of agreement returned to 0.51 and 1.28. CONCLUSIONS: PCCO needs frequent recalibration during hemorrhage and after vasopressor administration. (Anesth Analg 2007;105:107‐13)

Zeyuan Deng - One of the best experts on this subject based on the ideXlab platform.

  • investigation of lipid metabolism by a new structured lipid with medium and long chain triacylglycerols from cinnamomum camphora seed oil in healthy c57bl 6j mice
    Journal of Agricultural and Food Chemistry, 2018
    Co-Authors: Jinrong Shen, Chaoyue Xiong, Xuemei Zhu, Zeyuan Deng
    Abstract:

    In the present study, a new structured lipid with medium- and long-chain triacylglycerols (MLCTs) was synthesized from camellia oil (CO) and Cinnamomum camphora seed oil (CCSO) by enzymatic interesterification. Meanwhile, the antiobesity effects of structured lipid were investigated through observing the changes of enzymes related to lipid mobilization in healthy C57BL/6J mice. Results showed that after synthesis, the major triacylgeride (TAG) species of intesterificated product changed to LaCC/CLaC (12.6 ± 0.46%), LaCO/LCL (21.7 ± 0.76%), CCO/LaCL (14.2 ± 0.55%), COO/OCO (10.8 ± 0.43%), and OOO (18.6 ± 0.64%). Through second-stage molecular distillation, the purity of interesterified product (MLCT) achieved 95.6%. Later, male C57BL/6J mice were applied to study whether the new structured lipid with MLCT has the efficacy of preventing the formation of obesity or not. After feeding with different diets for 6 weeks, MLCTs could reduce body weight and fat deposition in adipose tissue, lower plasma triacylgly...

  • enzymatic synthesis of medium and long chain triacylglycerols enriched structured lipid from cinnamomum camphora seed oil and camellia oil by lipozyme rm im
    International Journal of Food Science and Technology, 2014
    Co-Authors: Manli Zhao, Yawei Fan, Xuemei Zhu, Zeyuan Deng
    Abstract:

    Summary Medium- and long-chain triacylglycerols (MLCTs)–enriched structured lipid (SL) was synthesised through enzymatic interesterification from Cinnamomum camphora seed oil (CCSO) and camellia oil (CO) using Lipozyme RM IM from Rhizomucor miehei as a biocatalyst. Effects of different reaction conditions including substrate molar ratio, reaction time and reaction temperature were investigated. Results showed that 55.81% of total MLCT species (CCO/LaCL, LaCO/LCL, COO/OCO and LaOO/OLaO) was obtained in the interesterified product under the optimal conditions of substrate molar ratio of 1:1.5 (CCSO/CO) at 60 °C for 3 h. Thereafter, fatty acid profiles, tOCOpherol contents and physiochemical characteristics of the interesterified product and physical blend were comparatively investigated. The fatty acid composition of the interesterified product consisted of capric acid (26.33%), lauric acid (21.29%) and oleic acid (42.33%). It should be mentioned that the interesterified product contained predominantly oleic acid (88.69%) at Sn-2 position, while MCFAs (68.05%) at Sn-1,3 positions. Compared with physical blend, the reduction in tOCOpherol contents and changes of physiochemical characteristics occurred in SL. The smoke point of the interesterified product was much higher than that of the physical blend, which meant that such MLCTs-enriched SL could be better for cooking purpose.

Christoph K. Hofer - One of the best experts on this subject based on the ideXlab platform.

  • Continuous cardiac output measurement by un-calibrated pulse wave analysis and pulmonary artery catheter in patients with septic shock
    Journal of Clinical Monitoring and Computing, 2016
    Co-Authors: Michael T. Ganter, Jamal A. Alhashemi, Adel M. Al-shabasy, Ursina M. Schmid, Peter Schott, Sanaa A. Shalabi, Ahmed M. Badri, Sonja Hartnack, Christoph K. Hofer
    Abstract:

    Septic shock is a serious medical condition. With increased concerns about invasive techniques, a number of non-invasive and semi-invasive devices measuring cardiac output (CO) have become commercially available. The aim of the present study was to determine the accuracy, precision and trending abilities of the FloTrac and the continuous pulmonary artery catheter thermodilution technique determining CO in septic shock patients. Consecutive septic shock patients were included in two centres and CO was measured every 4 h up to 48 h by FloTrac (APCO) and by pulmonary artery catheter (PAC) using the continuous (CCO) and intermittent (ICO) technique. Forty-seven septic shock patients with 326 matched sets of APCO, CCO and ICO data were available for analysis. Bland and Altman analysis revealed a mean bias ±2 SD of 0.0 ± 2.14 L min^−1 for APCO–ICO (%error = 34.5 %) and 0.23 ± 2.55 L min^−1 for CCO–ICO (%error = 40.4 %). Trend analysis showed a concordance of 85 and 81 % for APCO and CCO, respectively. In contrast to CCO, APCO was influenced by systemic vascular resistance and by mean arterial pressure. In septic shock patients, APCO measurements assessed by FloTrac but also the established CCO measurements using the PAC did not meet the currently accepted statistical criteria indicating acceptable clinical performance.

  • uncalibrated radial and femoral arterial pressure waveform analysis for continuous cardiac output measurement an evaluation in cardiac surgery patients
    Journal of Cardiothoracic and Vascular Anesthesia, 2010
    Co-Authors: Christoph K. Hofer, Danny Button, Luc Weibel, Michele Genoni, Andreas Zollinger
    Abstract:

    Objectives Arterial pressure waveform analysis is a less invasive alternative to the pulmonary artery catheter for continuous cardiac output (CO) measurement. Uncalibrated and calibrated systems are actually available (ie, the FloTrac/Vigileo system [Edwards Lifesciences, Irvine, CA] and the PiCCOplus system [Pulsion Medical Systems, Munich, Germany]). According to the FloTrac/Vigileo manufacturer, reliable measurements can be performed using any existing arterial catheter. The aim of this study was to evaluate CO determined by the FloTrac/Vigileo system using a radial (FCO radial ) and femoral arterial catheter (FCO femoral ) as well as the PiCCOplus system (PCO). Intermittent pulmonary artery thermodilution (ICO) was used as primary reference technique. Design A prospective clinical study. Setting A teaching hospital, single center. Participants Twenty-six cardiac surgery patients. Interventions Perioperative CO measurements. Measurements and Main Results CO was assessed at predefined measurement points. FCO radial , FCO femoral , and PCO were recorded after the induction of anesthesia, after sternotomy, at skin closure, after intensive care unit transfer, and during intensive care unit stay 12 and 24 hours after study initiation. ICO was determined as the mean of 3 bolus injections. Bland-Altman analysis revealed comparable mean bias and limits of agreement for FCO radial , FCO femoral , and PCO when compared with ICO. There was a decreased agreement for all devices in the postoperative period. However, a consistently close agreement was observed for the direct comparison between FCO radial and FCO femoral . Conclusions Performance of the FloTrac/Vigileo system via radial as well as femoral access and the PiCCOplus monitoring for cardiac output measurement were comparable when tested against intermittent thermodilution in cardiac surgery patients.

  • clinical evaluation of the flotrac vigileotm system and two established continuous cardiac output monitoring devices in patients undergoing cardiac surgery
    BJA: British Journal of Anaesthesia, 2007
    Co-Authors: Danny Button, Luc Weibel, Michele Genoni, Andreas Zollinger, O Reuthebuch, Christoph K. Hofer
    Abstract:

    Background Assessment of cardiac output (CO) by the FloTrac/Vigileo™ system may offer a less invasive means of determining the CO than either the pulmonary artery catheter (PAC) or the PiCCOplus™ system. The aim of this study was to compare CO measurements made using the FloTrac/Vigileo™ system with upgraded software (FCO, Edwards Lifesciences, Irvine CA, USA), the PiCCOplus™ system (PCO, Pulsion Medical Systems, Munich, Germany) and continuous CO monitoring using a PAC (CCO; Vigilance™ monitoring, Edwards Lifesciences, Irvine CA, USA) with intermittent pulmonary artery thermodilution (ICO). The study was conducted in patients undergoing elective cardiac surgery. Methods Thirty-one patients with preserved left ventricular function were enrolled. CCO, FCO, and PCO were recorded in the perioperative period at six predefined time points after achieving stable haemodynamic conditions; ICO was determined from the mean of three bolus injections. Bland–Altman analysis was used to compare CCO, FCO, and PCO with ICO. Results Bland–Altman analysis revealed a comparable mean bias and limits of agreement for all tested continuous CO monitoring devices using ICO as reference method. Agreement for all devices decreased in the postoperative period. Conclusion The performance of the FloTrac/Vigileo™ system, the PiCCOplus™, and the Vigilance™ CCO monitoring for CO measurement were comparable when tested against intermittent thermodilution in patients undergoing elective cardiac surgery.

Jonny Hobbhahn - One of the best experts on this subject based on the ideXlab platform.

  • continuous cardiac output measurement pulse contour analysis vs thermodilution technique in cardiac surgical patients
    BJA: British Journal of Anaesthesia, 1999
    Co-Authors: G Rodig, Christopher Prasser, Cornelius Keyl, A Liebold, Jonny Hobbhahn
    Abstract:

    We have analysed the clinical agreement between two methods of continuous cardiac output measurement pulse contour analysis (PCCO) and a continuous thermodilution technique (CCO), were both compared with the intermittent bolus thermodilution technique (BCO). Measurements were performed in 26 cardiac surgical patients (groups 1 and 2, 13 patients each, with an ejection fraction > 45% and < 45%, respectively) at 12 selected times. During operation, mean differences (bias) between PCCO-BCO and CCO-BCO did not differ in either group. However, phenylephrine-induced increases in systemic vascular resistance (SVR) by approximately 60% resulted in significant differences. Significantly higher absolute bias values of PCCO-BCO compared with CCO-BCO were also found early after operation in the ICU. Thus PCCO and CCO provided comparable measurements during coronary bypass surgery. After marked changes in SVR, further calibration of the PCCO device is necessary.

  • continuous cardiac output measurement pulse contour analysis vs thermodilution technique in cardiac surgical patients
    BJA: British Journal of Anaesthesia, 1999
    Co-Authors: G Rodig, Christopher Prasser, Cornelius Keyl, A Liebold, Jonny Hobbhahn
    Abstract:

    We have analysed the clinical agreement between two methods of continuous cardiac output measurement pulse contour analysis (PCCO) and a continuous thermodilution technique (CCO), were both compared with the intermittent bolus thermodilution technique (BCO). Measurements were performed in 26 cardiac surgical patients (groups 1 and 2, 13 patients each, with an ejection fraction > 45% and