The Experts below are selected from a list of 243270 Experts worldwide ranked by ideXlab platform
Yoichiro Ito - One of the best experts on this subject based on the ideXlab platform.
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isolation of high Purity casticin from artemisia annua l by high speed counter current chromatography
Journal of Chromatography A, 2007Co-Authors: Xiao Han, Tianyou Zhang, Yabin Zhang, Qinghui Liu, Yoichiro ItoAbstract:Abstract Following an initial clean-up step on silica, high-speed counter-current chromatography (HSCCC) was used to purify a flavone, casticin (5,3′-dihydroxy-3,6,7, 4′-tetramethoxyflavone), from an extract of the dried leaves of Artemisia annua L. The two-phase solvent system used was composed of n-hexane–ethyl acetate–methanol–water at an optimized volume ratio of 7:10:7:10 (v/v). HSCCC separation of 226.4 mg of crude sample (containing casticin at 16.5% Purity after silica gel clean-up) yielded 36.3 mg of casticin with a Purity of over 99% and 96.2% recovery. Identification of the target compound was performed by 1H NMR, 13C NMR, two-dimensional NMR, electrospray ionization MS, IR and UV.
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separation of salidroside from rhodiola crenulata by high speed counter current chromatography
Journal of Chromatography A, 2002Co-Authors: Xiao Han, Tianyou Zhang, Yun Wei, Xueli Cao, Yoichiro ItoAbstract:Abstract High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate–n-butanol–water (1:4:5, v/v) in the first run and chloroform–methanol–isopropanol–water (5:6:1:4) in the second run. The method yielded 21.9 mg of salidroside from 1.216 g of the crude sample at 98% Purity determined by HPLC analyses. Identification was performed by 1H NMR, 13C NMR, and MS.
Xiao Han - One of the best experts on this subject based on the ideXlab platform.
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isolation of high Purity casticin from artemisia annua l by high speed counter current chromatography
Journal of Chromatography A, 2007Co-Authors: Xiao Han, Tianyou Zhang, Yabin Zhang, Qinghui Liu, Yoichiro ItoAbstract:Abstract Following an initial clean-up step on silica, high-speed counter-current chromatography (HSCCC) was used to purify a flavone, casticin (5,3′-dihydroxy-3,6,7, 4′-tetramethoxyflavone), from an extract of the dried leaves of Artemisia annua L. The two-phase solvent system used was composed of n-hexane–ethyl acetate–methanol–water at an optimized volume ratio of 7:10:7:10 (v/v). HSCCC separation of 226.4 mg of crude sample (containing casticin at 16.5% Purity after silica gel clean-up) yielded 36.3 mg of casticin with a Purity of over 99% and 96.2% recovery. Identification of the target compound was performed by 1H NMR, 13C NMR, two-dimensional NMR, electrospray ionization MS, IR and UV.
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separation of salidroside from rhodiola crenulata by high speed counter current chromatography
Journal of Chromatography A, 2002Co-Authors: Xiao Han, Tianyou Zhang, Yun Wei, Xueli Cao, Yoichiro ItoAbstract:Abstract High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate–n-butanol–water (1:4:5, v/v) in the first run and chloroform–methanol–isopropanol–water (5:6:1:4) in the second run. The method yielded 21.9 mg of salidroside from 1.216 g of the crude sample at 98% Purity determined by HPLC analyses. Identification was performed by 1H NMR, 13C NMR, and MS.
Yun Wei - One of the best experts on this subject based on the ideXlab platform.
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preparative separation of bioactive compounds from essential oil of flaveria bidentis l kuntze using steam distillation extraction and one step high speed counter current chromatography
IEEE Journal of Solid-state Circuits, 2012Co-Authors: Yun WeiAbstract:In order to utilize and control the invasive weed, bioactive compounds from essential oil of Flaveria bidentis (L.) Kuntze were studied. Steam distillation extraction and one step high-speed counter-current chromatography were applied to separate and purify the caryophyllene oxide, 7,11-dimethyl-3-methylene-1,6,10-dodecatriene, and caryophyllene from essential oil of Flaveria bidentis (L.) Kuntze. The two-phase solvent system containing n-hexane/acetonitrile/ethanol (5:4:3, v/v/v) was selected for the one step separation mode according to the partition coefficient values (K) of the target compounds and the separation factor (α). The Purity of each isolated fraction after a single high-speed counter-current chromatography run was determined by high performance liquid chromatography. A 3.2 mg of caryophyllene oxide at a Purity of 92.6%, 10.4 mg of 7,11-dimethyl-3-methylene-1,6,10-dodecatriene at a Purity of 99.1% and 5.7 mg of caryophyllene at a Purity of 98.8% were obtained from 200 mg essential oil of Flaveria bidentis (L.) Kuntze. The chemical structures of these components were identified by GC-MS, (1) H-NMR, and (13) C-NMR.
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separation of salidroside from rhodiola crenulata by high speed counter current chromatography
Journal of Chromatography A, 2002Co-Authors: Xiao Han, Tianyou Zhang, Yun Wei, Xueli Cao, Yoichiro ItoAbstract:Abstract High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate–n-butanol–water (1:4:5, v/v) in the first run and chloroform–methanol–isopropanol–water (5:6:1:4) in the second run. The method yielded 21.9 mg of salidroside from 1.216 g of the crude sample at 98% Purity determined by HPLC analyses. Identification was performed by 1H NMR, 13C NMR, and MS.
Tianyou Zhang - One of the best experts on this subject based on the ideXlab platform.
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isolation of high Purity casticin from artemisia annua l by high speed counter current chromatography
Journal of Chromatography A, 2007Co-Authors: Xiao Han, Tianyou Zhang, Yabin Zhang, Qinghui Liu, Yoichiro ItoAbstract:Abstract Following an initial clean-up step on silica, high-speed counter-current chromatography (HSCCC) was used to purify a flavone, casticin (5,3′-dihydroxy-3,6,7, 4′-tetramethoxyflavone), from an extract of the dried leaves of Artemisia annua L. The two-phase solvent system used was composed of n-hexane–ethyl acetate–methanol–water at an optimized volume ratio of 7:10:7:10 (v/v). HSCCC separation of 226.4 mg of crude sample (containing casticin at 16.5% Purity after silica gel clean-up) yielded 36.3 mg of casticin with a Purity of over 99% and 96.2% recovery. Identification of the target compound was performed by 1H NMR, 13C NMR, two-dimensional NMR, electrospray ionization MS, IR and UV.
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separation of salidroside from rhodiola crenulata by high speed counter current chromatography
Journal of Chromatography A, 2002Co-Authors: Xiao Han, Tianyou Zhang, Yun Wei, Xueli Cao, Yoichiro ItoAbstract:Abstract High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate–n-butanol–water (1:4:5, v/v) in the first run and chloroform–methanol–isopropanol–water (5:6:1:4) in the second run. The method yielded 21.9 mg of salidroside from 1.216 g of the crude sample at 98% Purity determined by HPLC analyses. Identification was performed by 1H NMR, 13C NMR, and MS.
Ju Yi-hsu - One of the best experts on this subject based on the ideXlab platform.
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Separation and purification of cardol, cardanol and anacardic acid from cashew (Anacardium occidentale L.) nut-shell liquid using a simple two-step column chromatography
Taiwan Institute of Chemical Engineers, 2014Co-Authors: Yuliana Maria, Thi, Bich Thuyen Nguyen, Faika Sitti, Huynh, Lien Huong, Soetaredjo, Felycia Edi, Ju Yi-hsuAbstract:Long chain phenols contained in cashew nut shell liquid have important applications. Anacardic acids and cardols are extensively used in the pharmaceutical industry, while cardanols find use in resins, coating and polymer industries. In this work, a two-step reversed-phase silica gel column chromatography was employed to purify these major phenols in cashew nut shell liquid (CNSL). 6- Pentadecyl salicylic acid (C22H36O3, 98.8% Purity and 82.4% recovery), 5-pentadecyl resorcinol (C21H36O2, 99.3% Purity and 80.7% recovery) and 3-pentadecyl phenol (C21H36O, 99.2% Purity and 81.8% recovery) were successfully obtained and identified by 1H and 13C NMR, FTIR, mass spectrometry and elemental analysis
