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Yanling Wang - One of the best experts on this subject based on the ideXlab platform.
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Surgical model for Ocular Ischemic Syndrome in mice
Biomedical Research-tokyo, 2017Co-Authors: Yu Ling, Yanling WangAbstract:Background: To better understand the pathogenesis of Ocular Ischemic Syndrome (OIS), several animal models were established last decades. Our purpose is to characterize the functional and morphologic changes in a murine model of OIS caused by Bilateral Internal Carotid Artery Occlusion (BICAO). Methods: Adult mice were submitted to BICAO or sham surgery. Changes in Ocular blood flow and retinal circulation after surgery were evaluated by MRA to ensure the retinal blood flow occlusion. The retinal hypoperfusion was demonstrated 7 d after BICAO by both Ocular fundus photography and fluorescein angiography. The OCT images were taken to measure the thicknesses of the various retinal layers, and then eyes were enucleated and embedded in paraffin for morphological studies. Results: MRA images showed that the ligation of both internal carotid arteries significantly reduced Ocular blood flow and narrowed the blood vessels. BICAO for 7 d was explored by both Ocular fundus photography and fluorescein angiography finding that the retinal hypoperfusion persisted in the mice of BICAO group. The total retinal thickness and retinal ganglion cell density decreased compared with Sham group. However, no changes were evident in IPL layer in BICAO group, which was in agreement with the results obtained from OCT images. Conclusion: We successfully visualized the occlusion of blood flow after the BICAO by MRA, and angiography could also demonstrate that BICAO for 7 d was sufficient to maintain the retinal ischemia and induce the morphological changes. We suggest that this protocol might be used as a mouse model for OIS in the future.
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Role of RhoA/MERK1/ERK1/2/iNOS signaling in Ocular Ischemic Syndrome.
Graefe's Archive for Clinical and Experimental Ophthalmology, 2016Co-Authors: Jialin Wang, Yanling WangAbstract:To determine the molecular mechanisms underlying Ocular Ischemic Syndrome (OIS). This study uses a rat model to evaluate the role of the RhoA/MEK1/ ERK1/2/iNOS pathways in response to OIS-associated oxidative and nitrosative stress, with a long-term goal of identifying therapeutic targets for OIS. Rats were randomly allocated to one of three groups: bilateral occlusion of the common carotid artery (BOCCA), sham surgery control, or unoperated control (n = 8/group). Three months after the procedure, retinas were analyzed anatomically, using immunohistochemistry and by enzyme-linked immunosorbent assay for RhoA, MEK1, ERK1, ERK2, iNOS. Retinal injury was assessed using TUNEL. Levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured by WST-1 and TBA methods, respectively. In BOCCA rats, occlusion of the bilateral common carotid artery induced degeneration of retinal ganglion cells, which was not observed in either control group. Retinal levels of RhoA, MEK1, ERK1, ERK2, iNOS, NOX2, and MDA were elevated in the BOCCA group, but not in either control group. In comparison, retinal levels of SOD were reduced in SOD animals. By immunofluorescent staining, RhoA was elevated in all retinal layers, while the increased levels of MEK, ERK1/1, and NOX were restricted to the INL, and that of ERK1/2 and NOX inner nuclear layer; iNOS elevations were observed in both the inner and outer nuclear layers. TUNEL labeling results showed that BOCCA group is higher staining than sham and control group. OIS elevates activity of the RhoA/MERK1/ERK1/2/iNOS pathways throughout the retina, likely reflecting a response to oxidative and nitrosative stress. Retinal thickness was reduced in BOCCA rats, reflecting a loss of retinal ganglion cells following the reduced blood flow to the eye. These results indicate that drugs that inhibit these pathways may be effective treatments for OIS.
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role of rhoa merk1 erk1 2 inos signaling in Ocular Ischemic Syndrome
Graefes Archive for Clinical and Experimental Ophthalmology, 2016Co-Authors: Jialin Wang, Yanling WangAbstract:To determine the molecular mechanisms underlying Ocular Ischemic Syndrome (OIS). This study uses a rat model to evaluate the role of the RhoA/MEK1/ ERK1/2/iNOS pathways in response to OIS-associated oxidative and nitrosative stress, with a long-term goal of identifying therapeutic targets for OIS. Rats were randomly allocated to one of three groups: bilateral occlusion of the common carotid artery (BOCCA), sham surgery control, or unoperated control (n = 8/group). Three months after the procedure, retinas were analyzed anatomically, using immunohistochemistry and by enzyme-linked immunosorbent assay for RhoA, MEK1, ERK1, ERK2, iNOS. Retinal injury was assessed using TUNEL. Levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured by WST-1 and TBA methods, respectively. In BOCCA rats, occlusion of the bilateral common carotid artery induced degeneration of retinal ganglion cells, which was not observed in either control group. Retinal levels of RhoA, MEK1, ERK1, ERK2, iNOS, NOX2, and MDA were elevated in the BOCCA group, but not in either control group. In comparison, retinal levels of SOD were reduced in SOD animals. By immunofluorescent staining, RhoA was elevated in all retinal layers, while the increased levels of MEK, ERK1/1, and NOX were restricted to the INL, and that of ERK1/2 and NOX inner nuclear layer; iNOS elevations were observed in both the inner and outer nuclear layers. TUNEL labeling results showed that BOCCA group is higher staining than sham and control group. OIS elevates activity of the RhoA/MERK1/ERK1/2/iNOS pathways throughout the retina, likely reflecting a response to oxidative and nitrosative stress. Retinal thickness was reduced in BOCCA rats, reflecting a loss of retinal ganglion cells following the reduced blood flow to the eye. These results indicate that drugs that inhibit these pathways may be effective treatments for OIS.
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expression of bcl 2 and bax on retinal with a rat model for Ocular Ischemic Syndrome
Chinese journal of ophthalmology, 2015Co-Authors: Jialin Wang, Lu Zhao, Jintong Hou, Zhaoyang Meng, Yanling WangAbstract:Objective To tell the difference of Bcl-2 and Bax expression on the retina between the Ocular Ischemic Syndrome group and the sham group. Methods Experimental study. To establish the Ocular Ischemic Syndrome model by bilateral common carotid arteries obliteration (BCCAO) in BN rats. Twenty BN rats were divided into two groups randomly: 12 in the model group and 8 in the sham group. In model group, midline incised in the neck and bilateral common carotid artery obliterated. While in the sham group, midline incised in the neck with no obliteration. Pupillary reflex were detected in both groups. After 1 month, rats underwent fundus fluorescein angiography (FFA) examination. Optical coherence tomography (OCT) was used to measure and tell the retina thickness difference in both groups. Expression of Bcl-2 and Bax on rat retina and change of the Bcl- 2/Bax ratio were detected by immunohistochemical staining. Chisquare test was used for classification variable, two independent samples T test was used for quantitative data. Results Compared to the sham group, only 1 eye of BCCAO group maintained its direct pupillary reflex, other eyes of the group appeared either lost or weakened their direct pupillary reflex. However, all rats in the sham group maintained their pupillary reflex. FFA showed that in the BCCAO group, retinal circulation time was significantly longer than it was in the sham group (aterial phase:t=6.19,P 0.05) in Bcl- 2 expression in both groups, which was (5.61±0.98) μm in the model group and which was (5.63±0.88) μm in the sham group. And Bcl-2/Bax ratio was observed statistically significant decreased (P<0.05) in the BCCAO group compared with that in the sham group, which was (1.01 ± 0.23) in the model group, and which was (2.47±0.47) in the sham group. Conclusions BCCAO of rats lead to the injury of retina function, diminish of retinal thickness, imbalance of Bax and Bcl-2 expression. (Chin J Ophthalmol, 2015, 51: 912-917) Key words: Eye diseases; Ischemia; Retina; bcl-2-associated X protein; Fluorescein angiography; Disease models, animal
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Expression of Bcl-2 and Bax on retinal with a rat model for Ocular Ischemic Syndrome
[Zhonghua yan ke za zhi] Chinese journal of ophthalmology, 2015Co-Authors: Jialin Wang, Lu Zhao, Jintong Hou, Zhaoyang Meng, Yanling WangAbstract:Objective To tell the difference of Bcl-2 and Bax expression on the retina between the Ocular Ischemic Syndrome group and the sham group. Methods Experimental study. To establish the Ocular Ischemic Syndrome model by bilateral common carotid arteries obliteration (BCCAO) in BN rats. Twenty BN rats were divided into two groups randomly: 12 in the model group and 8 in the sham group. In model group, midline incised in the neck and bilateral common carotid artery obliterated. While in the sham group, midline incised in the neck with no obliteration. Pupillary reflex were detected in both groups. After 1 month, rats underwent fundus fluorescein angiography (FFA) examination. Optical coherence tomography (OCT) was used to measure and tell the retina thickness difference in both groups. Expression of Bcl-2 and Bax on rat retina and change of the Bcl- 2/Bax ratio were detected by immunohistochemical staining. Chisquare test was used for classification variable, two independent samples T test was used for quantitative data. Results Compared to the sham group, only 1 eye of BCCAO group maintained its direct pupillary reflex, other eyes of the group appeared either lost or weakened their direct pupillary reflex. However, all rats in the sham group maintained their pupillary reflex. FFA showed that in the BCCAO group, retinal circulation time was significantly longer than it was in the sham group (aterial phase:t=6.19,P 0.05) in Bcl- 2 expression in both groups, which was (5.61±0.98) μm in the model group and which was (5.63±0.88) μm in the sham group. And Bcl-2/Bax ratio was observed statistically significant decreased (P
Lippincott Williams Wilkins - One of the best experts on this subject based on the ideXlab platform.
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pearls oy sters Ocular Ischemic Syndrome
Neurology, 2012Co-Authors: Lippincott Williams WilkinsAbstract:In the Resident & Fellow article “Pearls & Oy-sters: Ocular Ischemic Syndrome” by R. Fernandez-Torron et al. ( Neurology ® 2012;79:e92–e94), there is an error in the text. Duplex carotid ultrasonography revealed occlusion of the initial portion of the right internal carotid artery (as shown in figure 2), not the right common carotid artery. The authors regret the error.
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Pearls & Oy-sters: Ocular Ischemic Syndrome
Neurology, 2012Co-Authors: Lippincott Williams WilkinsAbstract:In the Resident & Fellow article “Pearls & Oy-sters: Ocular Ischemic Syndrome” by R. Fernandez-Torron et al. ( Neurology ® 2012;79:e92–e94), there is an error in the text. Duplex carotid ultrasonography revealed occlusion of the initial portion of the right internal carotid artery (as shown in figure 2), not the right common carotid artery. The authors regret the error.
Jialin Wang - One of the best experts on this subject based on the ideXlab platform.
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Role of RhoA/MERK1/ERK1/2/iNOS signaling in Ocular Ischemic Syndrome.
Graefe's Archive for Clinical and Experimental Ophthalmology, 2016Co-Authors: Jialin Wang, Yanling WangAbstract:To determine the molecular mechanisms underlying Ocular Ischemic Syndrome (OIS). This study uses a rat model to evaluate the role of the RhoA/MEK1/ ERK1/2/iNOS pathways in response to OIS-associated oxidative and nitrosative stress, with a long-term goal of identifying therapeutic targets for OIS. Rats were randomly allocated to one of three groups: bilateral occlusion of the common carotid artery (BOCCA), sham surgery control, or unoperated control (n = 8/group). Three months after the procedure, retinas were analyzed anatomically, using immunohistochemistry and by enzyme-linked immunosorbent assay for RhoA, MEK1, ERK1, ERK2, iNOS. Retinal injury was assessed using TUNEL. Levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured by WST-1 and TBA methods, respectively. In BOCCA rats, occlusion of the bilateral common carotid artery induced degeneration of retinal ganglion cells, which was not observed in either control group. Retinal levels of RhoA, MEK1, ERK1, ERK2, iNOS, NOX2, and MDA were elevated in the BOCCA group, but not in either control group. In comparison, retinal levels of SOD were reduced in SOD animals. By immunofluorescent staining, RhoA was elevated in all retinal layers, while the increased levels of MEK, ERK1/1, and NOX were restricted to the INL, and that of ERK1/2 and NOX inner nuclear layer; iNOS elevations were observed in both the inner and outer nuclear layers. TUNEL labeling results showed that BOCCA group is higher staining than sham and control group. OIS elevates activity of the RhoA/MERK1/ERK1/2/iNOS pathways throughout the retina, likely reflecting a response to oxidative and nitrosative stress. Retinal thickness was reduced in BOCCA rats, reflecting a loss of retinal ganglion cells following the reduced blood flow to the eye. These results indicate that drugs that inhibit these pathways may be effective treatments for OIS.
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role of rhoa merk1 erk1 2 inos signaling in Ocular Ischemic Syndrome
Graefes Archive for Clinical and Experimental Ophthalmology, 2016Co-Authors: Jialin Wang, Yanling WangAbstract:To determine the molecular mechanisms underlying Ocular Ischemic Syndrome (OIS). This study uses a rat model to evaluate the role of the RhoA/MEK1/ ERK1/2/iNOS pathways in response to OIS-associated oxidative and nitrosative stress, with a long-term goal of identifying therapeutic targets for OIS. Rats were randomly allocated to one of three groups: bilateral occlusion of the common carotid artery (BOCCA), sham surgery control, or unoperated control (n = 8/group). Three months after the procedure, retinas were analyzed anatomically, using immunohistochemistry and by enzyme-linked immunosorbent assay for RhoA, MEK1, ERK1, ERK2, iNOS. Retinal injury was assessed using TUNEL. Levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured by WST-1 and TBA methods, respectively. In BOCCA rats, occlusion of the bilateral common carotid artery induced degeneration of retinal ganglion cells, which was not observed in either control group. Retinal levels of RhoA, MEK1, ERK1, ERK2, iNOS, NOX2, and MDA were elevated in the BOCCA group, but not in either control group. In comparison, retinal levels of SOD were reduced in SOD animals. By immunofluorescent staining, RhoA was elevated in all retinal layers, while the increased levels of MEK, ERK1/1, and NOX were restricted to the INL, and that of ERK1/2 and NOX inner nuclear layer; iNOS elevations were observed in both the inner and outer nuclear layers. TUNEL labeling results showed that BOCCA group is higher staining than sham and control group. OIS elevates activity of the RhoA/MERK1/ERK1/2/iNOS pathways throughout the retina, likely reflecting a response to oxidative and nitrosative stress. Retinal thickness was reduced in BOCCA rats, reflecting a loss of retinal ganglion cells following the reduced blood flow to the eye. These results indicate that drugs that inhibit these pathways may be effective treatments for OIS.
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expression of bcl 2 and bax on retinal with a rat model for Ocular Ischemic Syndrome
Chinese journal of ophthalmology, 2015Co-Authors: Jialin Wang, Lu Zhao, Jintong Hou, Zhaoyang Meng, Yanling WangAbstract:Objective To tell the difference of Bcl-2 and Bax expression on the retina between the Ocular Ischemic Syndrome group and the sham group. Methods Experimental study. To establish the Ocular Ischemic Syndrome model by bilateral common carotid arteries obliteration (BCCAO) in BN rats. Twenty BN rats were divided into two groups randomly: 12 in the model group and 8 in the sham group. In model group, midline incised in the neck and bilateral common carotid artery obliterated. While in the sham group, midline incised in the neck with no obliteration. Pupillary reflex were detected in both groups. After 1 month, rats underwent fundus fluorescein angiography (FFA) examination. Optical coherence tomography (OCT) was used to measure and tell the retina thickness difference in both groups. Expression of Bcl-2 and Bax on rat retina and change of the Bcl- 2/Bax ratio were detected by immunohistochemical staining. Chisquare test was used for classification variable, two independent samples T test was used for quantitative data. Results Compared to the sham group, only 1 eye of BCCAO group maintained its direct pupillary reflex, other eyes of the group appeared either lost or weakened their direct pupillary reflex. However, all rats in the sham group maintained their pupillary reflex. FFA showed that in the BCCAO group, retinal circulation time was significantly longer than it was in the sham group (aterial phase:t=6.19,P 0.05) in Bcl- 2 expression in both groups, which was (5.61±0.98) μm in the model group and which was (5.63±0.88) μm in the sham group. And Bcl-2/Bax ratio was observed statistically significant decreased (P<0.05) in the BCCAO group compared with that in the sham group, which was (1.01 ± 0.23) in the model group, and which was (2.47±0.47) in the sham group. Conclusions BCCAO of rats lead to the injury of retina function, diminish of retinal thickness, imbalance of Bax and Bcl-2 expression. (Chin J Ophthalmol, 2015, 51: 912-917) Key words: Eye diseases; Ischemia; Retina; bcl-2-associated X protein; Fluorescein angiography; Disease models, animal
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Expression of Bcl-2 and Bax on retinal with a rat model for Ocular Ischemic Syndrome
[Zhonghua yan ke za zhi] Chinese journal of ophthalmology, 2015Co-Authors: Jialin Wang, Lu Zhao, Jintong Hou, Zhaoyang Meng, Yanling WangAbstract:Objective To tell the difference of Bcl-2 and Bax expression on the retina between the Ocular Ischemic Syndrome group and the sham group. Methods Experimental study. To establish the Ocular Ischemic Syndrome model by bilateral common carotid arteries obliteration (BCCAO) in BN rats. Twenty BN rats were divided into two groups randomly: 12 in the model group and 8 in the sham group. In model group, midline incised in the neck and bilateral common carotid artery obliterated. While in the sham group, midline incised in the neck with no obliteration. Pupillary reflex were detected in both groups. After 1 month, rats underwent fundus fluorescein angiography (FFA) examination. Optical coherence tomography (OCT) was used to measure and tell the retina thickness difference in both groups. Expression of Bcl-2 and Bax on rat retina and change of the Bcl- 2/Bax ratio were detected by immunohistochemical staining. Chisquare test was used for classification variable, two independent samples T test was used for quantitative data. Results Compared to the sham group, only 1 eye of BCCAO group maintained its direct pupillary reflex, other eyes of the group appeared either lost or weakened their direct pupillary reflex. However, all rats in the sham group maintained their pupillary reflex. FFA showed that in the BCCAO group, retinal circulation time was significantly longer than it was in the sham group (aterial phase:t=6.19,P 0.05) in Bcl- 2 expression in both groups, which was (5.61±0.98) μm in the model group and which was (5.63±0.88) μm in the sham group. And Bcl-2/Bax ratio was observed statistically significant decreased (P
Yoshitomo Uchiyama - One of the best experts on this subject based on the ideXlab platform.
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Clinical Study Ocular Circulation and Chronic Ocular Ischemic Syndrome before and after Carotid Artery Revascularization Surgery
2016Co-Authors: Shoichiro Kawaguchi, Jun-ichi Iida, Yoshitomo UchiyamaAbstract:Copyright © 2012 Shoichiro Kawaguchi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. We evaluated the effect of carotid revascularization surgery on Ocular circulation and chronic Ocular Ischemic Syndrome (OIS). Methods. We examined ninety patients with carotid artery stenosis (more than 50 % stenosis) at its origin treated with carotid endarterectomy (N = 56) or carotid artery stenting (N = 34). Twenty-five patients (28%) complained of chronic OIS. Ocular circulation was examined before and after revascularization surgery using ophthalmic artery (OphAr) and central retinal artery (CRA) color Doppler flow imaging. Results. (1) Ocular circulation: preoperatively, the average OphAr peak systolic flow velocity (Vs) was 0.05m/sec, and the average CRA Vs was 0.07m/sec. At 1 week after surgery, the average OphAr Vs significantly increased to 0.32 (P < 0.05), and the average CRAVs significantly increased to 0.11m/sec (P < 0.05). These significant improvements were sustained throughout the three months of the followup. (2) OIS: during the follow-up period (mean: 3.6 years), 15 patients (60%) showed visual acuity improvement, and no patients complained of amaurosis fugax or worsening of the chronic OIS. Conclusion. Carotid revascularization surgery was effective in improving the Ocular circulation, and it was also useful for the chronic OIS due to the carotid artery stenosis. 1
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Ocular circulation and chronic Ocular Ischemic Syndrome before and after carotid artery revascularization surgery
Journal of Ophthalmology, 2012Co-Authors: Shoichiro Kawaguchi, Jun-ichi Iida, Yoshitomo UchiyamaAbstract:Background. We evaluated the effect of carotid revascularization surgery on Ocular circulation and chronic Ocular Ischemic Syndrome (OIS). Methods. We examined ninety patients with carotid artery stenosis (more than 50% stenosis) at its origin treated with carotid endarterectomy () or carotid artery stenting (). Twenty-five patients (28%) complained of chronic OIS. Ocular circulation was examined before and after revascularization surgery using ophthalmic artery (OphAr) and central retinal artery (CRA) color Doppler flow imaging. Results. (1) Ocular circulation: preoperatively, the average OphAr peak systolic flow velocity (Vs) was 0.05 m/sec, and the average CRA Vs was 0.07 m/sec. At 1 week after surgery, the average OphAr Vs significantly increased to 0.32 (), and the average CRA Vs significantly increased to 0.11 m/sec (). These significant improvements were sustained throughout the three months of the followup. (2) OIS: during the follow-up period (mean: 3.6 years), 15 patients (60%) showed visual acuity improvement, and no patients complained of amaurosis fugax or worsening of the chronic OIS. Conclusion. Carotid revascularization surgery was effective in improving the Ocular circulation, and it was also useful for the chronic OIS due to the carotid artery stenosis.
S P Park - One of the best experts on this subject based on the ideXlab platform.
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Diagnostic efficacy of total homocysteine and C-reactive protein for Ocular Ischemic Syndrome
Eye, 2011Co-Authors: I H Hong, J K Ahn, S Chang, S P ParkAbstract:Background To assess diagnostic efficacy of plasma total homocysteine (tHcy) and C-reactive protein (CRP) levels for Ocular Ischemic Syndrome (OIS). Methods In all, 87 patients with retinal vein occlusion (RVO), 955 patients with a stenosis of internal carotid artery (ICA) 90% were included between 2003 and 2009. A total of 43 patients with a stenosis ICA >90% were diagnosed as OIS. Fasting tHcy, CRP, lipid profiles, creatinine were measured, and diagnostic values of hyperhomocysteinemia or elevated CRP for OIS were evaluated. Result The mean plasma levels of tHcy (18.8 μ mol/l) and CRP (1.1 mmol/l) were the highest in patients with OIS among the groups. The prevalences of hyperhomocysteinemia (72%) and elevated CRP (77%) were the highest in OIS among the groups. In patients with stenosis of ICA, the diagnostic sensitivity/specificity for OIS was 70/79% in hyperhomocysteinemia and 73/73% in elevated CRP. The diagnostic sensitivity and specificity for OIS were 53 and 86% in both hyperhomocysteinemia and elevated CRP. The lipid profiles and creatinine levels were similar among the groups. Conclusion Our results suggest that hyperhomocysteinemia and elevated CRP may be associated with the development of OIS. The measurements of tHcy and CRP in blood may help to assist the diagnosis of OIS in a stenosis of ICA.
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Diagnostic efficacy of total homocysteine and C-reactive protein for Ocular Ischemic Syndrome
Eye, 2011Co-Authors: I H Hong, J K Ahn, Seong-sil Chang, S P ParkAbstract:To assess diagnostic efficacy of plasma total homocysteine (tHcy) and C-reactive protein (CRP) levels for Ocular Ischemic Syndrome (OIS). In all, 87 patients with retinal vein occlusion (RVO), 955 patients with a stenosis of internal carotid artery (ICA) 90% were included between 2003 and 2009. A total of 43 patients with a stenosis ICA >90% were diagnosed as OIS. Fasting tHcy, CRP, lipid profiles, creatinine were measured, and diagnostic values of hyperhomocysteinemia or elevated CRP for OIS were evaluated. The mean plasma levels of tHcy (18.8 μmol/l) and CRP (1.1 mmol/l) were the highest in patients with OIS among the groups. The prevalences of hyperhomocysteinemia (72%) and elevated CRP (77%) were the highest in OIS among the groups. In patients with stenosis of ICA, the diagnostic sensitivity/specificity for OIS was 70/79% in hyperhomocysteinemia and 73/73% in elevated CRP. The diagnostic sensitivity and specificity for OIS were 53 and 86% in both hyperhomocysteinemia and elevated CRP. The lipid profiles and creatinine levels were similar among the groups. Our results suggest that hyperhomocysteinemia and elevated CRP may be associated with the development of OIS. The measurements of tHcy and CRP in blood may help to assist the diagnosis of OIS in a stenosis of ICA.
