The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Ville Santala - One of the best experts on this subject based on the ideXlab platform.
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Real-Time monitoring of intracellular wax ester metabolism
Microbial Cell Factories, 2011Co-Authors: Suvi Santala, Elena Efimova, Matti Karp, Ville SantalaAbstract:Background Wax esters are industrially relevant molecules exploited in several applications of Oleochemistry and food industry. At the moment, the production processes mostly rely on chemical synthesis from rather expensive starting materials, and therefore solutions are sought from biotechnology. Bacterial wax esters are attractive alternatives, and especially the wax ester metabolism of Acinetobacter sp. has been extensively studied. However, the lack of suitable tools for rapid and simple monitoring of wax ester metabolism in vivo has partly restricted the screening and analyses of potential hosts and optimal conditions.
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Real-Time monitoring of intracellular wax ester metabolism
Microbial Cell Factories, 2011Co-Authors: Suvi Santala, Elena Efimova, Matti Karp, Ville SantalaAbstract:Background Wax esters are industrially relevant molecules exploited in several applications of Oleochemistry and food industry. At the moment, the production processes mostly rely on chemical synthesis from rather expensive starting materials, and therefore solutions are sought from biotechnology. Bacterial wax esters are attractive alternatives, and especially the wax ester metabolism of Acinetobacter sp. has been extensively studied. However, the lack of suitable tools for rapid and simple monitoring of wax ester metabolism in vivo has partly restricted the screening and analyses of potential hosts and optimal conditions. Results Based on sensitive and specific detection of intracellular long-chain aldehydes, specific intermediates of wax ester synthesis, bacterial luciferase (LuxAB) was exploited in studying the wax ester metabolism in Acinetobacter baylyi ADP1. Luminescence was detected in the cultivation of the strain producing wax esters, and the changes in signal levels could be linked to corresponding cell growth and wax ester synthesis phases. Conclusions The monitoring system showed correlation between wax ester synthesis pattern and luminescent signal. The system shows potential for real-time screening purposes and studies on bacterial wax esters, revealing new aspects to dynamics and role of wax ester metabolism in bacteria.
Suvi Santala - One of the best experts on this subject based on the ideXlab platform.
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Real-Time monitoring of intracellular wax ester metabolism
Microbial Cell Factories, 2011Co-Authors: Suvi Santala, Elena Efimova, Matti Karp, Ville SantalaAbstract:Background Wax esters are industrially relevant molecules exploited in several applications of Oleochemistry and food industry. At the moment, the production processes mostly rely on chemical synthesis from rather expensive starting materials, and therefore solutions are sought from biotechnology. Bacterial wax esters are attractive alternatives, and especially the wax ester metabolism of Acinetobacter sp. has been extensively studied. However, the lack of suitable tools for rapid and simple monitoring of wax ester metabolism in vivo has partly restricted the screening and analyses of potential hosts and optimal conditions.
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Real-Time monitoring of intracellular wax ester metabolism
Microbial Cell Factories, 2011Co-Authors: Suvi Santala, Elena Efimova, Matti Karp, Ville SantalaAbstract:Background Wax esters are industrially relevant molecules exploited in several applications of Oleochemistry and food industry. At the moment, the production processes mostly rely on chemical synthesis from rather expensive starting materials, and therefore solutions are sought from biotechnology. Bacterial wax esters are attractive alternatives, and especially the wax ester metabolism of Acinetobacter sp. has been extensively studied. However, the lack of suitable tools for rapid and simple monitoring of wax ester metabolism in vivo has partly restricted the screening and analyses of potential hosts and optimal conditions. Results Based on sensitive and specific detection of intracellular long-chain aldehydes, specific intermediates of wax ester synthesis, bacterial luciferase (LuxAB) was exploited in studying the wax ester metabolism in Acinetobacter baylyi ADP1. Luminescence was detected in the cultivation of the strain producing wax esters, and the changes in signal levels could be linked to corresponding cell growth and wax ester synthesis phases. Conclusions The monitoring system showed correlation between wax ester synthesis pattern and luminescent signal. The system shows potential for real-time screening purposes and studies on bacterial wax esters, revealing new aspects to dynamics and role of wax ester metabolism in bacteria.
Elena Efimova - One of the best experts on this subject based on the ideXlab platform.
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Real-Time monitoring of intracellular wax ester metabolism
Microbial Cell Factories, 2011Co-Authors: Suvi Santala, Elena Efimova, Matti Karp, Ville SantalaAbstract:Background Wax esters are industrially relevant molecules exploited in several applications of Oleochemistry and food industry. At the moment, the production processes mostly rely on chemical synthesis from rather expensive starting materials, and therefore solutions are sought from biotechnology. Bacterial wax esters are attractive alternatives, and especially the wax ester metabolism of Acinetobacter sp. has been extensively studied. However, the lack of suitable tools for rapid and simple monitoring of wax ester metabolism in vivo has partly restricted the screening and analyses of potential hosts and optimal conditions.
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Real-Time monitoring of intracellular wax ester metabolism
Microbial Cell Factories, 2011Co-Authors: Suvi Santala, Elena Efimova, Matti Karp, Ville SantalaAbstract:Background Wax esters are industrially relevant molecules exploited in several applications of Oleochemistry and food industry. At the moment, the production processes mostly rely on chemical synthesis from rather expensive starting materials, and therefore solutions are sought from biotechnology. Bacterial wax esters are attractive alternatives, and especially the wax ester metabolism of Acinetobacter sp. has been extensively studied. However, the lack of suitable tools for rapid and simple monitoring of wax ester metabolism in vivo has partly restricted the screening and analyses of potential hosts and optimal conditions. Results Based on sensitive and specific detection of intracellular long-chain aldehydes, specific intermediates of wax ester synthesis, bacterial luciferase (LuxAB) was exploited in studying the wax ester metabolism in Acinetobacter baylyi ADP1. Luminescence was detected in the cultivation of the strain producing wax esters, and the changes in signal levels could be linked to corresponding cell growth and wax ester synthesis phases. Conclusions The monitoring system showed correlation between wax ester synthesis pattern and luminescent signal. The system shows potential for real-time screening purposes and studies on bacterial wax esters, revealing new aspects to dynamics and role of wax ester metabolism in bacteria.
Matti Karp - One of the best experts on this subject based on the ideXlab platform.
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Real-Time monitoring of intracellular wax ester metabolism
Microbial Cell Factories, 2011Co-Authors: Suvi Santala, Elena Efimova, Matti Karp, Ville SantalaAbstract:Background Wax esters are industrially relevant molecules exploited in several applications of Oleochemistry and food industry. At the moment, the production processes mostly rely on chemical synthesis from rather expensive starting materials, and therefore solutions are sought from biotechnology. Bacterial wax esters are attractive alternatives, and especially the wax ester metabolism of Acinetobacter sp. has been extensively studied. However, the lack of suitable tools for rapid and simple monitoring of wax ester metabolism in vivo has partly restricted the screening and analyses of potential hosts and optimal conditions.
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Real-Time monitoring of intracellular wax ester metabolism
Microbial Cell Factories, 2011Co-Authors: Suvi Santala, Elena Efimova, Matti Karp, Ville SantalaAbstract:Background Wax esters are industrially relevant molecules exploited in several applications of Oleochemistry and food industry. At the moment, the production processes mostly rely on chemical synthesis from rather expensive starting materials, and therefore solutions are sought from biotechnology. Bacterial wax esters are attractive alternatives, and especially the wax ester metabolism of Acinetobacter sp. has been extensively studied. However, the lack of suitable tools for rapid and simple monitoring of wax ester metabolism in vivo has partly restricted the screening and analyses of potential hosts and optimal conditions. Results Based on sensitive and specific detection of intracellular long-chain aldehydes, specific intermediates of wax ester synthesis, bacterial luciferase (LuxAB) was exploited in studying the wax ester metabolism in Acinetobacter baylyi ADP1. Luminescence was detected in the cultivation of the strain producing wax esters, and the changes in signal levels could be linked to corresponding cell growth and wax ester synthesis phases. Conclusions The monitoring system showed correlation between wax ester synthesis pattern and luminescent signal. The system shows potential for real-time screening purposes and studies on bacterial wax esters, revealing new aspects to dynamics and role of wax ester metabolism in bacteria.
Oznur Kose - One of the best experts on this subject based on the ideXlab platform.
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immobilized candida antarctica lipase catalyzed alcoholysis of cotton seed oil in a solvent free medium
Bioresource Technology, 2002Co-Authors: Oznur Kose, Melek Tüter, Ayse H AksoyAbstract:Abstract The alcoholysis (transesterification) of the refined cotton seed oil of Turkish origin with primary and secondary alcohols was investigated in the presence of an immobilized enzyme from Candida antarctica , commercially called Novozym 435 in a solvent-free medium. The optimum conditions of the methanolysis were as follows: 30% enzyme based on oil weight; oil/alcohol molar ratio 1:4; temperature: 50 °C and reaction time: 7 h. Maximum methyl esters (ME) yield was 91.5%. At the same conditions cotton seed oil was converted with short-chain primary and secondary alcohols to its corresponding esters with conversions between 72% and 94%. Our results indicated that alcoholysis products of cotton seed oil could be used as valuable intermediates in Oleochemistry.
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Immobilized Candida antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium.
Bioresource technology, 2002Co-Authors: Oznur Kose, Melek Tüter, H. Ayşe AksoyAbstract:The alcoholysis (transesterification) of the refined cotton seed oil of Turkish origin with primary and secondary alcohols was investigated in the presence of an immobilized enzyme from Candida antarctica, commercially called Novozym 435 in a solvent-free medium. The optimum conditions of the methanolysis were as follows: 30% enzyme based on oil weight; oil/alcohol molar ratio 1:4; temperature: 50 degrees C and reaction time: 7 h. Maximum methyl esters (ME) yield was 91.5%. At the same conditions cotton seed oil was converted with short-chain primary and secondary alcohols to its corresponding esters with conversions between 72% and 94%. Our results indicated that alcoholysis products of cotton seed oil could be used as valuable intermediates in Oleochemistry.
