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Anne Osbourn - One of the best experts on this subject based on the ideXlab platform.
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identification and characterization of the iridoid synthase involved in Oleuropein biosynthesis in olive olea europaea fruits
Journal of Biological Chemistry, 2016Co-Authors: Fiammetta Alagna, Fernando Geuflores, Hajo Kries, Francesco Panara, Luciana Baldoni, Sarah E Oconnor, Anne OsbournAbstract:Abstract The secoiridoids are the main class of specialized metabolites present in olive (Olea europaea L.) fruit. In particular, the secoiridoid Oleuropein strongly influences olive oil quality due to its bitterness, which is a desirable trait. In addition, Oleuropein possesses a wide range of pharmacological properties, including antioxidant, antiinflammatory, and anti-cancer activities. In accordance, obtaining high-Oleuropein varieties is a main goal of molecular breeding programs. Here we use a transcriptomic approach to identify candidate genes belonging to the secoiridoid pathway in olive. From these candidates, we have functionally characterized the olive homologue of iridoid synthase (OeISY), an unusual terpene cyclase that couples an NAD(P)H-dependent 1,4-reduction step with a subsequent cyclization, and we provide evidence that OeISY likely generates the monoterpene scaffold of Oleuropein in olive fruits. OeISY, the first pathway gene characterised for this type of secoiridoid, is a potential target for breeding programs in a high value secoiridoid-accumulating species.
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identification and characterization of the iridoid synthase involved in Oleuropein biosynthesis in olive olea europaea fruits
Journal of Biological Chemistry, 2016Co-Authors: Fiammetta Alagna, Fernando Geuflores, Hajo Kries, Francesco Panara, Luciana Baldoni, Sarah E Oconnor, Anne OsbournAbstract:The secoiridoids are the main class of specialized metabolites present in olive (Olea europaea L.) fruit. In particular, the secoiridoid Oleuropein strongly influences olive oil quality because of its bitterness, which is a desirable trait. In addition, Oleuropein possesses a wide range of pharmacological properties, including antioxidant, anti-inflammatory, and anti-cancer activities. In accordance, obtaining high Oleuropein varieties is a main goal of molecular breeding programs. Here we use a transcriptomic approach to identify candidate genes belonging to the secoiridoid pathway in olive. From these candidates, we have functionally characterized the olive homologue of iridoid synthase (OeISY), an unusual terpene cyclase that couples an NAD (P)H-dependent 1,4-reduction step with a subsequent cyclization, and we provide evidence that OeISY likely generates the monoterpene scaffold of Oleuropein in olive fruits. OeISY, the first pathway gene characterized for this type of secoiridoid, is a potential target for breeding programs in a high value secoiridoid-accumulating species.
Daniela Impellizzeri - One of the best experts on this subject based on the ideXlab platform.
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the effects of a polyphenol present in olive oil Oleuropein aglycone in an experimental model of spinal cord injury in mice
Biochemical Pharmacology, 2012Co-Authors: Daniela Impellizzeri, Enzo Perri, Emanuela Esposito, Emanuela Mazzon, Irene Paterniti, Rosanna Di Paola, Placido Bramanti, Valeria Maria Morittu, Antonio Procopio, Domenico BrittiAbstract:Several olive oil phenolic compounds, such us Oleuropein have attracted considerable attention because of their antioxidant activity, anti-atherosclerotic and anti-inflammatory properties. The aim of this experimental study was to determine the effect of Oleuropein aglycone, a hydrolysis product of Oleuropein, in the inflammatory response, in particular in the secondary injury associated with the mouse model of spinal cord trauma. The injury was induced by application of vascular clips to the dura via a four-level T5-T8 laminectomy in mice. Oleuropein aglycone was administered in mice (100 μg/kg, 40 μg/kg, 20 μg/kg, 10% ethanol, i.p.) 1h and 6h after the trauma. The treatment with Oleuropein aglycone significantly decreased: (1) histological damage, (2) motor recovery, (3) nuclear factor (NF)-κB expression and IKB-α degradation, (4) protein kinase A (PKA) activity and expression, (5) pro-inflammatory cytokines production such as tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β), 6) inducible nitric oxide synthase (iNOS) expression, (7) neutrophil infiltration, (8) lipid peroxidation, (9) nitrotyrosine and poly-ADP-ribose (PAR) formation, (10) glial cell-derived neurotrophic factor (GDNF) levels, (11) apoptosis (TUNEL staining, FAS ligand expression, Caspase 3, Bax and Bcl-2 expression). Thus, we propose that olive oil phenolic constituents such as Oleuropein aglycone may be useful in the treatment of various inflammatory diseases.
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Oleuropein aglycone an olive oil compound ameliorates development of arthritis caused by injection of collagen type ii in mice
Journal of Pharmacology and Experimental Therapeutics, 2011Co-Authors: Daniela Impellizzeri, Emanuela Esposito, Emanuela Mazzon, Irene Paterniti, Rosanna Di Paola, Valeria Maria Morittu, Antonio Procopio, Domenico Britti, Salvatore CuzzocreaAbstract:The aim of this study was to investigate the effect of Oleuropein aglycone, an olive oil compound, on the modulation of the inflammatory response in mice subjected to collagen-induced arthritis (CIA). CIA was induced in mice by an intradermal injection of 100 μl of an emulsion containing 100 μg of bovine type II collagen (CII) and complete Freund's adjuvant (CFA) at the base of the tail. On day 21, a second injection of CII in CFA was administered. Mice developed erosive hind paw arthritis when immunized with CII in CFA. Macroscopic clinical evidence of CIA first appeared as periarticular erythema and edema in the hind paws. The incidence of CIA was 100% by day 28 in the CII-challenged mice and the severity of CIA progressed over a 35-day period with resorption of bone. The histopathology of CIA included erosion of the cartilage at the joint. Treatment with Oleuropein aglycone starting at the onset of arthritis (day 25) ameliorated the clinical signs at days 26 to 35 and improved histological status in the joint and paw. The degree of oxidative and nitrosative damage was also significantly reduced in Oleuropein aglycone-treated mice. Plasma levels of the proinflammatory cytokines were also significantly reduced by Oleuropein aglycone. In addition, we have confirmed the beneficial effects of Oleuropein aglycone on an experimental model of CIA in a therapeutic regimen of post-treatment, with treatment started at day 28, demonstrating that Oleuropein aglycone exerts an anti-inflammatory effect during chronic inflammation and ameliorates the tissue damage associated with CIA.
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the effects of Oleuropein aglycone an olive oil compound in a mouse model of carrageenan induced pleurisy
Clinical Nutrition, 2011Co-Authors: Daniela Impellizzeri, Emanuela Esposito, Emanuela Mazzon, Irene Paterniti, Rosanna Di Paola, Placido Bramanti, Valeria Maria Morittu, Domenico Britti, A Procopio, Salvatore CuzzocreaAbstract:Summary Background & aims Several olive oil phenolic compounds, such us Oleuropein have attracted considerable attention because of their antioxidant activity, anti-atherosclerotic and anti-inflammatory properties. The aim of this study was to investigate the effects of Oleuropein aglycone, a hydrolysis product of Oleuropein, in a mouse model of carrageenan-induced pleurisy. Methods Mice were anaesthetized and subjected to a skin incision at the level of the left sixth intercostals space. The underlying muscle was dissected and saline or saline containing 2% λ-carrageenan was injected into the pleural cavity. Results Injection of carrageenan elicited an acute inflammatory response characterized by: infiltration of neutrophils in lung tissues ( P P P P P P P P P P Conclusions Thus, we propose that olive oil phenolic constituents may be useful in the treatment of various inflammatory diseases.
Salvatore Cuzzocrea - One of the best experts on this subject based on the ideXlab platform.
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Oleuropein aglycone an olive oil compound ameliorates development of arthritis caused by injection of collagen type ii in mice
Journal of Pharmacology and Experimental Therapeutics, 2011Co-Authors: Daniela Impellizzeri, Emanuela Esposito, Emanuela Mazzon, Irene Paterniti, Rosanna Di Paola, Valeria Maria Morittu, Antonio Procopio, Domenico Britti, Salvatore CuzzocreaAbstract:The aim of this study was to investigate the effect of Oleuropein aglycone, an olive oil compound, on the modulation of the inflammatory response in mice subjected to collagen-induced arthritis (CIA). CIA was induced in mice by an intradermal injection of 100 μl of an emulsion containing 100 μg of bovine type II collagen (CII) and complete Freund's adjuvant (CFA) at the base of the tail. On day 21, a second injection of CII in CFA was administered. Mice developed erosive hind paw arthritis when immunized with CII in CFA. Macroscopic clinical evidence of CIA first appeared as periarticular erythema and edema in the hind paws. The incidence of CIA was 100% by day 28 in the CII-challenged mice and the severity of CIA progressed over a 35-day period with resorption of bone. The histopathology of CIA included erosion of the cartilage at the joint. Treatment with Oleuropein aglycone starting at the onset of arthritis (day 25) ameliorated the clinical signs at days 26 to 35 and improved histological status in the joint and paw. The degree of oxidative and nitrosative damage was also significantly reduced in Oleuropein aglycone-treated mice. Plasma levels of the proinflammatory cytokines were also significantly reduced by Oleuropein aglycone. In addition, we have confirmed the beneficial effects of Oleuropein aglycone on an experimental model of CIA in a therapeutic regimen of post-treatment, with treatment started at day 28, demonstrating that Oleuropein aglycone exerts an anti-inflammatory effect during chronic inflammation and ameliorates the tissue damage associated with CIA.
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the effects of Oleuropein aglycone an olive oil compound in a mouse model of carrageenan induced pleurisy
Clinical Nutrition, 2011Co-Authors: Daniela Impellizzeri, Emanuela Esposito, Emanuela Mazzon, Irene Paterniti, Rosanna Di Paola, Placido Bramanti, Valeria Maria Morittu, Domenico Britti, A Procopio, Salvatore CuzzocreaAbstract:Summary Background & aims Several olive oil phenolic compounds, such us Oleuropein have attracted considerable attention because of their antioxidant activity, anti-atherosclerotic and anti-inflammatory properties. The aim of this study was to investigate the effects of Oleuropein aglycone, a hydrolysis product of Oleuropein, in a mouse model of carrageenan-induced pleurisy. Methods Mice were anaesthetized and subjected to a skin incision at the level of the left sixth intercostals space. The underlying muscle was dissected and saline or saline containing 2% λ-carrageenan was injected into the pleural cavity. Results Injection of carrageenan elicited an acute inflammatory response characterized by: infiltration of neutrophils in lung tissues ( P P P P P P P P P P Conclusions Thus, we propose that olive oil phenolic constituents may be useful in the treatment of various inflammatory diseases.
Domenico Britti - One of the best experts on this subject based on the ideXlab platform.
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the effects of a polyphenol present in olive oil Oleuropein aglycone in an experimental model of spinal cord injury in mice
Biochemical Pharmacology, 2012Co-Authors: Daniela Impellizzeri, Enzo Perri, Emanuela Esposito, Emanuela Mazzon, Irene Paterniti, Rosanna Di Paola, Placido Bramanti, Valeria Maria Morittu, Antonio Procopio, Domenico BrittiAbstract:Several olive oil phenolic compounds, such us Oleuropein have attracted considerable attention because of their antioxidant activity, anti-atherosclerotic and anti-inflammatory properties. The aim of this experimental study was to determine the effect of Oleuropein aglycone, a hydrolysis product of Oleuropein, in the inflammatory response, in particular in the secondary injury associated with the mouse model of spinal cord trauma. The injury was induced by application of vascular clips to the dura via a four-level T5-T8 laminectomy in mice. Oleuropein aglycone was administered in mice (100 μg/kg, 40 μg/kg, 20 μg/kg, 10% ethanol, i.p.) 1h and 6h after the trauma. The treatment with Oleuropein aglycone significantly decreased: (1) histological damage, (2) motor recovery, (3) nuclear factor (NF)-κB expression and IKB-α degradation, (4) protein kinase A (PKA) activity and expression, (5) pro-inflammatory cytokines production such as tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β), 6) inducible nitric oxide synthase (iNOS) expression, (7) neutrophil infiltration, (8) lipid peroxidation, (9) nitrotyrosine and poly-ADP-ribose (PAR) formation, (10) glial cell-derived neurotrophic factor (GDNF) levels, (11) apoptosis (TUNEL staining, FAS ligand expression, Caspase 3, Bax and Bcl-2 expression). Thus, we propose that olive oil phenolic constituents such as Oleuropein aglycone may be useful in the treatment of various inflammatory diseases.
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Oleuropein aglycone an olive oil compound ameliorates development of arthritis caused by injection of collagen type ii in mice
Journal of Pharmacology and Experimental Therapeutics, 2011Co-Authors: Daniela Impellizzeri, Emanuela Esposito, Emanuela Mazzon, Irene Paterniti, Rosanna Di Paola, Valeria Maria Morittu, Antonio Procopio, Domenico Britti, Salvatore CuzzocreaAbstract:The aim of this study was to investigate the effect of Oleuropein aglycone, an olive oil compound, on the modulation of the inflammatory response in mice subjected to collagen-induced arthritis (CIA). CIA was induced in mice by an intradermal injection of 100 μl of an emulsion containing 100 μg of bovine type II collagen (CII) and complete Freund's adjuvant (CFA) at the base of the tail. On day 21, a second injection of CII in CFA was administered. Mice developed erosive hind paw arthritis when immunized with CII in CFA. Macroscopic clinical evidence of CIA first appeared as periarticular erythema and edema in the hind paws. The incidence of CIA was 100% by day 28 in the CII-challenged mice and the severity of CIA progressed over a 35-day period with resorption of bone. The histopathology of CIA included erosion of the cartilage at the joint. Treatment with Oleuropein aglycone starting at the onset of arthritis (day 25) ameliorated the clinical signs at days 26 to 35 and improved histological status in the joint and paw. The degree of oxidative and nitrosative damage was also significantly reduced in Oleuropein aglycone-treated mice. Plasma levels of the proinflammatory cytokines were also significantly reduced by Oleuropein aglycone. In addition, we have confirmed the beneficial effects of Oleuropein aglycone on an experimental model of CIA in a therapeutic regimen of post-treatment, with treatment started at day 28, demonstrating that Oleuropein aglycone exerts an anti-inflammatory effect during chronic inflammation and ameliorates the tissue damage associated with CIA.
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the effects of Oleuropein aglycone an olive oil compound in a mouse model of carrageenan induced pleurisy
Clinical Nutrition, 2011Co-Authors: Daniela Impellizzeri, Emanuela Esposito, Emanuela Mazzon, Irene Paterniti, Rosanna Di Paola, Placido Bramanti, Valeria Maria Morittu, Domenico Britti, A Procopio, Salvatore CuzzocreaAbstract:Summary Background & aims Several olive oil phenolic compounds, such us Oleuropein have attracted considerable attention because of their antioxidant activity, anti-atherosclerotic and anti-inflammatory properties. The aim of this study was to investigate the effects of Oleuropein aglycone, a hydrolysis product of Oleuropein, in a mouse model of carrageenan-induced pleurisy. Methods Mice were anaesthetized and subjected to a skin incision at the level of the left sixth intercostals space. The underlying muscle was dissected and saline or saline containing 2% λ-carrageenan was injected into the pleural cavity. Results Injection of carrageenan elicited an acute inflammatory response characterized by: infiltration of neutrophils in lung tissues ( P P P P P P P P P P Conclusions Thus, we propose that olive oil phenolic constituents may be useful in the treatment of various inflammatory diseases.
Enzo Perri - One of the best experts on this subject based on the ideXlab platform.
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the effects of a polyphenol present in olive oil Oleuropein aglycone in an experimental model of spinal cord injury in mice
Biochemical Pharmacology, 2012Co-Authors: Daniela Impellizzeri, Enzo Perri, Emanuela Esposito, Emanuela Mazzon, Irene Paterniti, Rosanna Di Paola, Placido Bramanti, Valeria Maria Morittu, Antonio Procopio, Domenico BrittiAbstract:Several olive oil phenolic compounds, such us Oleuropein have attracted considerable attention because of their antioxidant activity, anti-atherosclerotic and anti-inflammatory properties. The aim of this experimental study was to determine the effect of Oleuropein aglycone, a hydrolysis product of Oleuropein, in the inflammatory response, in particular in the secondary injury associated with the mouse model of spinal cord trauma. The injury was induced by application of vascular clips to the dura via a four-level T5-T8 laminectomy in mice. Oleuropein aglycone was administered in mice (100 μg/kg, 40 μg/kg, 20 μg/kg, 10% ethanol, i.p.) 1h and 6h after the trauma. The treatment with Oleuropein aglycone significantly decreased: (1) histological damage, (2) motor recovery, (3) nuclear factor (NF)-κB expression and IKB-α degradation, (4) protein kinase A (PKA) activity and expression, (5) pro-inflammatory cytokines production such as tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β), 6) inducible nitric oxide synthase (iNOS) expression, (7) neutrophil infiltration, (8) lipid peroxidation, (9) nitrotyrosine and poly-ADP-ribose (PAR) formation, (10) glial cell-derived neurotrophic factor (GDNF) levels, (11) apoptosis (TUNEL staining, FAS ligand expression, Caspase 3, Bax and Bcl-2 expression). Thus, we propose that olive oil phenolic constituents such as Oleuropein aglycone may be useful in the treatment of various inflammatory diseases.
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quantitation of Oleuropein in virgin olive oil by ionspray mass spectrometry selected reaction monitoring
Journal of Agricultural and Food Chemistry, 1999Co-Authors: Enzo Perri, And Andrea Raffaelli, Giovanni SindonaAbstract:This work describes the unambiguous evaluation of the presence of Oleuropein in virgin olive oils by ionspray tandem mass spectrometry (ISI-MS/MS). The oil samples obtained from different cultivars, such as Carolea, Cassanese, Coratina, Dolce di Rossano, Roggianella, and Tonda di Strongoli, grown in different geographical areas of Calabria, Italy, have shown an average content of Oleuropein ranging from 1 ppb to 11 ppm. Commercial virgin oil samples, blended in some cases, contain significant amounts of this pharmacologically important antioxidant. The MS/MS methodology was applied in a triple-quadrupole instrument, through continuous scanning of the third analyzer to detect Oleuropein in methanol extracts and in selected ion monitoring (SRM) for its quantitative assay. Keywords: Oleuropein; virgin olive oil; quantitative assay of Oleuropein; ionspray mass spectrometry; selected reaction monitoring (SRM)
