The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform
Jose A.l. Arruda - One of the best experts on this subject based on the ideXlab platform.
-
Omentum facilitates liver regeneration.
World journal of gastroenterology, 2009Co-Authors: Ashok Kumar Singh, Nishit Pancholi, Jilpa Patel, Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, Mark A. Kraus, George Dunea, Jose A.l. ArrudaAbstract:To investigate the mechanism of liver regeneration induced by fusing the Omentum to a small traumatic injury created in the liver. We studied three groups of rats. In one group the rats were omentectomized; in another group the Omentum was left in situ and was not activated, and in the third group the Omentum was activated by polydextran particles. We pre-activated the Omentum by injecting polydextran particles and then made a small wedge wound in the rat liver to allow the Omentum to fuse to the wound. We monitored the regeneration of the liver by determining the ratio of liver weight/body weight, by histological evaluation (including immune staining for cytokeratin-19, an oval cell marker), and by testing for developmental gene activation using reverse transcription polymerase chain reaction (RT-PCR). There was no liver regeneration in the omentectomized rats, nor was there significant regeneration when the Omentum was not activated, even though in this instance the Omentum had fused with the liver. In contrast, the liver in the rats with the activated Omentum expanded to a size 50% greater than the original, and there was histologically an interlying tissue between the wounded liver and the activated Omentum in which bile ducts, containing cytokeratin-19 positive oval cells, extended from the wound edge. In this interlying tissue, oval cells were abundant and appeared to proliferate to form new liver tissue. In rats pre-treated with drugs that inhibited hepatocyte growth, liver proliferation was ongoing, indicating that regeneration of the liver was the result of oval cell expansion. Activated Omentum facilitates liver regeneration following injury by a mechanism that depends largely on oval cell proliferation.
-
Omentum facilitates liver regeneration
World Journal of Gastroenterology, 2009Co-Authors: Ashok Kumar Singh, Nishit Pancholi, Jilpa Patel, Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, Mark A. Kraus, George Dunea, Jose A.l. ArrudaAbstract:CONCLUSION: Activated Omentum facilitates liver regeneration following injury by a mechanism that depends largely on oval cell proliferation.
-
activated Omentum becomes rich in factors that promote healing and tissue regeneration
Cell and Tissue Research, 2007Co-Authors: Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, George Dunea, Jose A.l. Arruda, Ashok Kumar SinghAbstract:In order to study the mechanism by which an omental pedicle promotes healing when applied to an injured site, we injected a foreign body into the abdominal cavity to activate the Omentum. One week after the injection, we isolated the Omentum and measured blood vessel density, blood content, growth and angiogenesis factors (VEGF and others), chemotactic factors (SDF-1α), and progenitor cells (CXCR-4, WT-1). We found that the native Omentum, which consisted mostly of adipose tissue, expanded the mass of its non-adipose part (milky spots) 15– to 20-fold. VEGF and other growth factors increased by two– to four-fold, blood vessel density by three-fold, and blood content by two-fold. The activated Omentum also showed increases in SDF-1α, CXCR-4, and WT-1 cells (factors and cells positively associated with tissue regeneration). Thus, we propose that an Omentum activated by a foreign body (or by injury) greatly expands its milky-spot tissue and becomes rich in growth factors and progenitor cells that facilitate the healing and regeneration of injured tissue.
Ashok Kumar Singh - One of the best experts on this subject based on the ideXlab platform.
-
Omentum facilitates liver regeneration.
World journal of gastroenterology, 2009Co-Authors: Ashok Kumar Singh, Nishit Pancholi, Jilpa Patel, Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, Mark A. Kraus, George Dunea, Jose A.l. ArrudaAbstract:To investigate the mechanism of liver regeneration induced by fusing the Omentum to a small traumatic injury created in the liver. We studied three groups of rats. In one group the rats were omentectomized; in another group the Omentum was left in situ and was not activated, and in the third group the Omentum was activated by polydextran particles. We pre-activated the Omentum by injecting polydextran particles and then made a small wedge wound in the rat liver to allow the Omentum to fuse to the wound. We monitored the regeneration of the liver by determining the ratio of liver weight/body weight, by histological evaluation (including immune staining for cytokeratin-19, an oval cell marker), and by testing for developmental gene activation using reverse transcription polymerase chain reaction (RT-PCR). There was no liver regeneration in the omentectomized rats, nor was there significant regeneration when the Omentum was not activated, even though in this instance the Omentum had fused with the liver. In contrast, the liver in the rats with the activated Omentum expanded to a size 50% greater than the original, and there was histologically an interlying tissue between the wounded liver and the activated Omentum in which bile ducts, containing cytokeratin-19 positive oval cells, extended from the wound edge. In this interlying tissue, oval cells were abundant and appeared to proliferate to form new liver tissue. In rats pre-treated with drugs that inhibited hepatocyte growth, liver proliferation was ongoing, indicating that regeneration of the liver was the result of oval cell expansion. Activated Omentum facilitates liver regeneration following injury by a mechanism that depends largely on oval cell proliferation.
-
Omentum facilitates liver regeneration
World Journal of Gastroenterology, 2009Co-Authors: Ashok Kumar Singh, Nishit Pancholi, Jilpa Patel, Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, Mark A. Kraus, George Dunea, Jose A.l. ArrudaAbstract:CONCLUSION: Activated Omentum facilitates liver regeneration following injury by a mechanism that depends largely on oval cell proliferation.
-
activated Omentum becomes rich in factors that promote healing and tissue regeneration
Cell and Tissue Research, 2007Co-Authors: Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, George Dunea, Jose A.l. Arruda, Ashok Kumar SinghAbstract:In order to study the mechanism by which an omental pedicle promotes healing when applied to an injured site, we injected a foreign body into the abdominal cavity to activate the Omentum. One week after the injection, we isolated the Omentum and measured blood vessel density, blood content, growth and angiogenesis factors (VEGF and others), chemotactic factors (SDF-1α), and progenitor cells (CXCR-4, WT-1). We found that the native Omentum, which consisted mostly of adipose tissue, expanded the mass of its non-adipose part (milky spots) 15– to 20-fold. VEGF and other growth factors increased by two– to four-fold, blood vessel density by three-fold, and blood content by two-fold. The activated Omentum also showed increases in SDF-1α, CXCR-4, and WT-1 cells (factors and cells positively associated with tissue regeneration). Thus, we propose that an Omentum activated by a foreign body (or by injury) greatly expands its milky-spot tissue and becomes rich in growth factors and progenitor cells that facilitate the healing and regeneration of injured tissue.
Natalia O. Litbarg - One of the best experts on this subject based on the ideXlab platform.
-
Omentum facilitates liver regeneration.
World journal of gastroenterology, 2009Co-Authors: Ashok Kumar Singh, Nishit Pancholi, Jilpa Patel, Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, Mark A. Kraus, George Dunea, Jose A.l. ArrudaAbstract:To investigate the mechanism of liver regeneration induced by fusing the Omentum to a small traumatic injury created in the liver. We studied three groups of rats. In one group the rats were omentectomized; in another group the Omentum was left in situ and was not activated, and in the third group the Omentum was activated by polydextran particles. We pre-activated the Omentum by injecting polydextran particles and then made a small wedge wound in the rat liver to allow the Omentum to fuse to the wound. We monitored the regeneration of the liver by determining the ratio of liver weight/body weight, by histological evaluation (including immune staining for cytokeratin-19, an oval cell marker), and by testing for developmental gene activation using reverse transcription polymerase chain reaction (RT-PCR). There was no liver regeneration in the omentectomized rats, nor was there significant regeneration when the Omentum was not activated, even though in this instance the Omentum had fused with the liver. In contrast, the liver in the rats with the activated Omentum expanded to a size 50% greater than the original, and there was histologically an interlying tissue between the wounded liver and the activated Omentum in which bile ducts, containing cytokeratin-19 positive oval cells, extended from the wound edge. In this interlying tissue, oval cells were abundant and appeared to proliferate to form new liver tissue. In rats pre-treated with drugs that inhibited hepatocyte growth, liver proliferation was ongoing, indicating that regeneration of the liver was the result of oval cell expansion. Activated Omentum facilitates liver regeneration following injury by a mechanism that depends largely on oval cell proliferation.
-
Omentum facilitates liver regeneration
World Journal of Gastroenterology, 2009Co-Authors: Ashok Kumar Singh, Nishit Pancholi, Jilpa Patel, Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, Mark A. Kraus, George Dunea, Jose A.l. ArrudaAbstract:CONCLUSION: Activated Omentum facilitates liver regeneration following injury by a mechanism that depends largely on oval cell proliferation.
-
activated Omentum becomes rich in factors that promote healing and tissue regeneration
Cell and Tissue Research, 2007Co-Authors: Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, George Dunea, Jose A.l. Arruda, Ashok Kumar SinghAbstract:In order to study the mechanism by which an omental pedicle promotes healing when applied to an injured site, we injected a foreign body into the abdominal cavity to activate the Omentum. One week after the injection, we isolated the Omentum and measured blood vessel density, blood content, growth and angiogenesis factors (VEGF and others), chemotactic factors (SDF-1α), and progenitor cells (CXCR-4, WT-1). We found that the native Omentum, which consisted mostly of adipose tissue, expanded the mass of its non-adipose part (milky spots) 15– to 20-fold. VEGF and other growth factors increased by two– to four-fold, blood vessel density by three-fold, and blood content by two-fold. The activated Omentum also showed increases in SDF-1α, CXCR-4, and WT-1 cells (factors and cells positively associated with tissue regeneration). Thus, we propose that an Omentum activated by a foreign body (or by injury) greatly expands its milky-spot tissue and becomes rich in growth factors and progenitor cells that facilitate the healing and regeneration of injured tissue.
Krishnamurthy P. Gudehithlu - One of the best experts on this subject based on the ideXlab platform.
-
Omentum facilitates liver regeneration.
World journal of gastroenterology, 2009Co-Authors: Ashok Kumar Singh, Nishit Pancholi, Jilpa Patel, Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, Mark A. Kraus, George Dunea, Jose A.l. ArrudaAbstract:To investigate the mechanism of liver regeneration induced by fusing the Omentum to a small traumatic injury created in the liver. We studied three groups of rats. In one group the rats were omentectomized; in another group the Omentum was left in situ and was not activated, and in the third group the Omentum was activated by polydextran particles. We pre-activated the Omentum by injecting polydextran particles and then made a small wedge wound in the rat liver to allow the Omentum to fuse to the wound. We monitored the regeneration of the liver by determining the ratio of liver weight/body weight, by histological evaluation (including immune staining for cytokeratin-19, an oval cell marker), and by testing for developmental gene activation using reverse transcription polymerase chain reaction (RT-PCR). There was no liver regeneration in the omentectomized rats, nor was there significant regeneration when the Omentum was not activated, even though in this instance the Omentum had fused with the liver. In contrast, the liver in the rats with the activated Omentum expanded to a size 50% greater than the original, and there was histologically an interlying tissue between the wounded liver and the activated Omentum in which bile ducts, containing cytokeratin-19 positive oval cells, extended from the wound edge. In this interlying tissue, oval cells were abundant and appeared to proliferate to form new liver tissue. In rats pre-treated with drugs that inhibited hepatocyte growth, liver proliferation was ongoing, indicating that regeneration of the liver was the result of oval cell expansion. Activated Omentum facilitates liver regeneration following injury by a mechanism that depends largely on oval cell proliferation.
-
Omentum facilitates liver regeneration
World Journal of Gastroenterology, 2009Co-Authors: Ashok Kumar Singh, Nishit Pancholi, Jilpa Patel, Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, Mark A. Kraus, George Dunea, Jose A.l. ArrudaAbstract:CONCLUSION: Activated Omentum facilitates liver regeneration following injury by a mechanism that depends largely on oval cell proliferation.
-
activated Omentum becomes rich in factors that promote healing and tissue regeneration
Cell and Tissue Research, 2007Co-Authors: Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, George Dunea, Jose A.l. Arruda, Ashok Kumar SinghAbstract:In order to study the mechanism by which an omental pedicle promotes healing when applied to an injured site, we injected a foreign body into the abdominal cavity to activate the Omentum. One week after the injection, we isolated the Omentum and measured blood vessel density, blood content, growth and angiogenesis factors (VEGF and others), chemotactic factors (SDF-1α), and progenitor cells (CXCR-4, WT-1). We found that the native Omentum, which consisted mostly of adipose tissue, expanded the mass of its non-adipose part (milky spots) 15– to 20-fold. VEGF and other growth factors increased by two– to four-fold, blood vessel density by three-fold, and blood content by two-fold. The activated Omentum also showed increases in SDF-1α, CXCR-4, and WT-1 cells (factors and cells positively associated with tissue regeneration). Thus, we propose that an Omentum activated by a foreign body (or by injury) greatly expands its milky-spot tissue and becomes rich in growth factors and progenitor cells that facilitate the healing and regeneration of injured tissue.
Perianna Sethupathi - One of the best experts on this subject based on the ideXlab platform.
-
Omentum facilitates liver regeneration.
World journal of gastroenterology, 2009Co-Authors: Ashok Kumar Singh, Nishit Pancholi, Jilpa Patel, Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, Mark A. Kraus, George Dunea, Jose A.l. ArrudaAbstract:To investigate the mechanism of liver regeneration induced by fusing the Omentum to a small traumatic injury created in the liver. We studied three groups of rats. In one group the rats were omentectomized; in another group the Omentum was left in situ and was not activated, and in the third group the Omentum was activated by polydextran particles. We pre-activated the Omentum by injecting polydextran particles and then made a small wedge wound in the rat liver to allow the Omentum to fuse to the wound. We monitored the regeneration of the liver by determining the ratio of liver weight/body weight, by histological evaluation (including immune staining for cytokeratin-19, an oval cell marker), and by testing for developmental gene activation using reverse transcription polymerase chain reaction (RT-PCR). There was no liver regeneration in the omentectomized rats, nor was there significant regeneration when the Omentum was not activated, even though in this instance the Omentum had fused with the liver. In contrast, the liver in the rats with the activated Omentum expanded to a size 50% greater than the original, and there was histologically an interlying tissue between the wounded liver and the activated Omentum in which bile ducts, containing cytokeratin-19 positive oval cells, extended from the wound edge. In this interlying tissue, oval cells were abundant and appeared to proliferate to form new liver tissue. In rats pre-treated with drugs that inhibited hepatocyte growth, liver proliferation was ongoing, indicating that regeneration of the liver was the result of oval cell expansion. Activated Omentum facilitates liver regeneration following injury by a mechanism that depends largely on oval cell proliferation.
-
Omentum facilitates liver regeneration
World Journal of Gastroenterology, 2009Co-Authors: Ashok Kumar Singh, Nishit Pancholi, Jilpa Patel, Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, Mark A. Kraus, George Dunea, Jose A.l. ArrudaAbstract:CONCLUSION: Activated Omentum facilitates liver regeneration following injury by a mechanism that depends largely on oval cell proliferation.
-
activated Omentum becomes rich in factors that promote healing and tissue regeneration
Cell and Tissue Research, 2007Co-Authors: Natalia O. Litbarg, Krishnamurthy P. Gudehithlu, Perianna Sethupathi, George Dunea, Jose A.l. Arruda, Ashok Kumar SinghAbstract:In order to study the mechanism by which an omental pedicle promotes healing when applied to an injured site, we injected a foreign body into the abdominal cavity to activate the Omentum. One week after the injection, we isolated the Omentum and measured blood vessel density, blood content, growth and angiogenesis factors (VEGF and others), chemotactic factors (SDF-1α), and progenitor cells (CXCR-4, WT-1). We found that the native Omentum, which consisted mostly of adipose tissue, expanded the mass of its non-adipose part (milky spots) 15– to 20-fold. VEGF and other growth factors increased by two– to four-fold, blood vessel density by three-fold, and blood content by two-fold. The activated Omentum also showed increases in SDF-1α, CXCR-4, and WT-1 cells (factors and cells positively associated with tissue regeneration). Thus, we propose that an Omentum activated by a foreign body (or by injury) greatly expands its milky-spot tissue and becomes rich in growth factors and progenitor cells that facilitate the healing and regeneration of injured tissue.