The Experts below are selected from a list of 645 Experts worldwide ranked by ideXlab platform
Yahong Geng - One of the best experts on this subject based on the ideXlab platform.
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sequential phototrophic mixotrophic cultivation of oleaginous microalga graesiella sp wbg 1 in a 1000 m 2 Open Raceway Pond
Biotechnology for Biofuels, 2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia Liu, Yahong GengAbstract:Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale Open Ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon. Laboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic–mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in Open Raceway Ponds. Graesiella sp. WBG-1 maximally produced 12.5 g biomass and 4.1 g lipids m−2 day−1 in SPMC in a 1000 m2 Raceway Pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97 × 105 CFU ml−1) is comparable to that of the phototrophic cultivations. SPMC is an effective and feasible method to cultivate lipid-rich microalgae in Open Raceway Ponds. Successful scale-up of SPMC in a commercial Raceway Pond (1000 m2 culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and β-carotene.
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isolation and characterization of an endoparasite from the culture of oleaginous microalga graesiella sp wbg 1
Algal Research-Biomass Biofuels and Bioproducts, 2017Co-Authors: Yi Ding, Zhongjie Wang, Xina Peng, Yahong GengAbstract:Abstract Oleaginous microalgae are an important potential feedstock for biodiesel production. However, a problem in mass cultivation of microalgae is the frequent occurrence of infection by algal parasites. In the present study, an endoparasite WZ01 was isolated from an Open Raceway Pond of oleaginous Graesiella sp. WBG-1, and caused epidemics resulting in algal population collapse. According to 18S rDNA-based phylogenetic analyses, morphology, ultrastructure and life cycle, strain WZ01 was identified as a member of Aphelidea, Amoeboaphelidium protococcarum , although it had some minor differences from A . protococcarum X-5 and FD95. Examination via transmission electron microscopy demonstrated that zoospores of the parasite WZ01 were amoeboid, which can produce two types of pseudopodia (multiple filopodia and short anterior lamellipodium), but the pseudocilium was not observed. Interestingly, amoeboid zoospores contained numerous dense-body vesicles, which have not been previously described. Moreover, our results revealed the presence of a microtubule inside the penetration tube, indicating that the contents of the cyst were injected into the host not only by vacuole pressure but also by using a microtubule-mediated mechanism. In addition, of the 42 tested algal species, only cultures of coccoid green algae Chlorococcum sp. A213 and Chlorococcum sp. GP1 rapidly and intensively developed infections by parasite WZ01. This is the first report on endoparasitic infection in oleaginous coccoid green algae. Our results will enhance understanding of parasite–host relationships, which will be beneficial in developing strategies for infection control.
Caixia Liu - One of the best experts on this subject based on the ideXlab platform.
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Sequential phototrophic–mixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000 m 2 Open Raceway Pond
Biotechnology for biofuels, 2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia LiuAbstract:Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale Open Ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon. Laboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic–mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in Open Raceway Ponds. Graesiella sp. WBG-1 maximally produced 12.5 g biomass and 4.1 g lipids m−2 day−1 in SPMC in a 1000 m2 Raceway Pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97 × 105 CFU ml−1) is comparable to that of the phototrophic cultivations. SPMC is an effective and feasible method to cultivate lipid-rich microalgae in Open Raceway Ponds. Successful scale-up of SPMC in a commercial Raceway Pond (1000 m2 culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and β-carotene.
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MOESM3 of Sequential phototrophicâmixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000Â m2 Open Raceway Pond
2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia LiuAbstract:Additional file 3. Changes of residual nitrate and phosphate during the Graesiella cultivation in Open Raceway Pond
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Sequential phototrophic–mixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000 m2 Open Raceway Pond
BMC, 2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia LiuAbstract:Abstract Background Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale Open Ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon. Results Laboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic–mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in Open Raceway Ponds. Graesiella sp. WBG-1 maximally produced 12.5 g biomass and 4.1 g lipids m−2 day−1 in SPMC in a 1000 m2 Raceway Pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97 × 105 CFU ml−1) is comparable to that of the phototrophic cultivations. Conclusions SPMC is an effective and feasible method to cultivate lipid-rich microalgae in Open Raceway Ponds. Successful scale-up of SPMC in a commercial Raceway Pond (1000 m2 culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and β-carotene
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sequential phototrophic mixotrophic cultivation of oleaginous microalga graesiella sp wbg 1 in a 1000 m 2 Open Raceway Pond
Biotechnology for Biofuels, 2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia Liu, Yahong GengAbstract:Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale Open Ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon. Laboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic–mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in Open Raceway Ponds. Graesiella sp. WBG-1 maximally produced 12.5 g biomass and 4.1 g lipids m−2 day−1 in SPMC in a 1000 m2 Raceway Pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97 × 105 CFU ml−1) is comparable to that of the phototrophic cultivations. SPMC is an effective and feasible method to cultivate lipid-rich microalgae in Open Raceway Ponds. Successful scale-up of SPMC in a commercial Raceway Pond (1000 m2 culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and β-carotene.
Yi Ding - One of the best experts on this subject based on the ideXlab platform.
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Sequential phototrophic–mixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000 m 2 Open Raceway Pond
Biotechnology for biofuels, 2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia LiuAbstract:Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale Open Ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon. Laboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic–mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in Open Raceway Ponds. Graesiella sp. WBG-1 maximally produced 12.5 g biomass and 4.1 g lipids m−2 day−1 in SPMC in a 1000 m2 Raceway Pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97 × 105 CFU ml−1) is comparable to that of the phototrophic cultivations. SPMC is an effective and feasible method to cultivate lipid-rich microalgae in Open Raceway Ponds. Successful scale-up of SPMC in a commercial Raceway Pond (1000 m2 culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and β-carotene.
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MOESM3 of Sequential phototrophicâmixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000Â m2 Open Raceway Pond
2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia LiuAbstract:Additional file 3. Changes of residual nitrate and phosphate during the Graesiella cultivation in Open Raceway Pond
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Sequential phototrophic–mixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000 m2 Open Raceway Pond
BMC, 2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia LiuAbstract:Abstract Background Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale Open Ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon. Results Laboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic–mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in Open Raceway Ponds. Graesiella sp. WBG-1 maximally produced 12.5 g biomass and 4.1 g lipids m−2 day−1 in SPMC in a 1000 m2 Raceway Pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97 × 105 CFU ml−1) is comparable to that of the phototrophic cultivations. Conclusions SPMC is an effective and feasible method to cultivate lipid-rich microalgae in Open Raceway Ponds. Successful scale-up of SPMC in a commercial Raceway Pond (1000 m2 culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and β-carotene
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sequential phototrophic mixotrophic cultivation of oleaginous microalga graesiella sp wbg 1 in a 1000 m 2 Open Raceway Pond
Biotechnology for Biofuels, 2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia Liu, Yahong GengAbstract:Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale Open Ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon. Laboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic–mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in Open Raceway Ponds. Graesiella sp. WBG-1 maximally produced 12.5 g biomass and 4.1 g lipids m−2 day−1 in SPMC in a 1000 m2 Raceway Pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97 × 105 CFU ml−1) is comparable to that of the phototrophic cultivations. SPMC is an effective and feasible method to cultivate lipid-rich microalgae in Open Raceway Ponds. Successful scale-up of SPMC in a commercial Raceway Pond (1000 m2 culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and β-carotene.
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isolation and characterization of an endoparasite from the culture of oleaginous microalga graesiella sp wbg 1
Algal Research-Biomass Biofuels and Bioproducts, 2017Co-Authors: Yi Ding, Zhongjie Wang, Xina Peng, Yahong GengAbstract:Abstract Oleaginous microalgae are an important potential feedstock for biodiesel production. However, a problem in mass cultivation of microalgae is the frequent occurrence of infection by algal parasites. In the present study, an endoparasite WZ01 was isolated from an Open Raceway Pond of oleaginous Graesiella sp. WBG-1, and caused epidemics resulting in algal population collapse. According to 18S rDNA-based phylogenetic analyses, morphology, ultrastructure and life cycle, strain WZ01 was identified as a member of Aphelidea, Amoeboaphelidium protococcarum , although it had some minor differences from A . protococcarum X-5 and FD95. Examination via transmission electron microscopy demonstrated that zoospores of the parasite WZ01 were amoeboid, which can produce two types of pseudopodia (multiple filopodia and short anterior lamellipodium), but the pseudocilium was not observed. Interestingly, amoeboid zoospores contained numerous dense-body vesicles, which have not been previously described. Moreover, our results revealed the presence of a microtubule inside the penetration tube, indicating that the contents of the cyst were injected into the host not only by vacuole pressure but also by using a microtubule-mediated mechanism. In addition, of the 42 tested algal species, only cultures of coccoid green algae Chlorococcum sp. A213 and Chlorococcum sp. GP1 rapidly and intensively developed infections by parasite WZ01. This is the first report on endoparasitic infection in oleaginous coccoid green algae. Our results will enhance understanding of parasite–host relationships, which will be beneficial in developing strategies for infection control.
Wei Cong - One of the best experts on this subject based on the ideXlab platform.
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Installation of flow deflectors and wing baffles to reduce dead zone and enhance flashing light effect in an Open Raceway Pond
Bioresource technology, 2015Co-Authors: Qinghua Zhang, Shengzhang Xue, Chenghu Yan, Shumei Wen, Wei CongAbstract:To reduce the dead zone and enhance the flashing light effect, a novel Open Raceway Pond with flow deflectors and wing baffles was developed. The hydrodynamics and light characteristics in the novel Open Raceway Pond were investigated using computational fluid dynamics. Results showed that, compared with the control Pond, pressure loss in the flow channel of the Pond with optimized flow deflectors decreased by 14.58%, average fluid velocity increased by 26.89% and dead zone decreased by 60.42%. With wing baffles built into the Raceway Pond, significant swirling flow was produced. Moreover, the period of average L/D cycle was shortened. In outdoor cultivation of freshwater Chlorella sp., the biomass concentration of Chlorella sp. cultivated in the Raceway Pond with wing baffles was 30.11% higher than that of the control Pond.
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evaluation of power consumption of paddle wheel in an Open Raceway Pond
Bioprocess and Biosystems Engineering, 2014Co-Authors: Yanxi Li, Xia Wu, Zhihui Wang, Qinghua Zhang, Wei CongAbstract:Open Raceway Ponds are widely adopted in microalgae cultivation. Paddle wheels consume the most part of power during the process of cultivation in Open Raceway Ponds. The configuration of blades directly determines power consumption for paddle wheels. In this work, power consumption of four blades configurations was determined in a bench-scale Open Raceway Pond of 2.2 m(2). The effect of blades configuration, the influence of filling levels from 5 to 15 cm and influence of rotational speeds from 7 to 15 r min(-1) on shaft power consumption (P (S)), fluid velocity (U (c)) and paddle wheel efficiency (eta) was investigated. Results demonstrated that flat blades were the most efficient configuration. Higher culture depth led to larger U (c), more P (s) and larger eta, especially when blades were not totally immerged in water. Under the same filling level and rotational speed, the value of P (S) decreases in the order: zigzagged, flat, forward-curved and back-curved, respectively. The zigzagged blades led to a larger U (c) at the culture depth of 5 cm, while flat and forward-curved blades drove a larger U (c) when culture depth was higher than 5 cm. The maximum value of eta was 0.50 with flat blades at 11 r min(-1) and 15 cm of culture depth. Empirical correlations of non-dimensional numbers related to operation parameters and blades geometry for four paddle wheel blades were also proposed.
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Evaluation of power consumption of paddle wheel in an Open Raceway Pond.
Bioprocess and biosystems engineering, 2013Co-Authors: Li Yanxi, Qinghua Zhang, Zhihui Wang, Wei CongAbstract:Open Raceway Ponds are widely adopted in microalgae cultivation. Paddle wheels consume the most part of power during the process of cultivation in Open Raceway Ponds. The configuration of blades directly determines power consumption for paddle wheels. In this work, power consumption of four blades configurations was determined in a bench-scale Open Raceway Pond of 2.2 m(2). The effect of blades configuration, the influence of filling levels from 5 to 15 cm and influence of rotational speeds from 7 to 15 r min(-1) on shaft power consumption (P(S)), fluid velocity (U(c)) and paddle wheel efficiency (η) was investigated. Results demonstrated that flat blades were the most efficient configuration. Higher culture depth led to larger U(c), more P(s) and larger η, especially when blades were not totally immerged in water. Under the same filling level and rotational speed, the value of P(S) decreases in the order: zigzagged, flat, forward-curved and back-curved, respectively. The zigzagged blades led to a larger U(c) at the culture depth of 5 cm, while flat and forward-curved blades drove a larger U(c) when culture depth was higher than 5 cm. The maximum value of η was 0.50 with flat blades at 11 r min(-1) and 15 cm of culture depth. Empirical correlations of non-dimensional numbers related to operation parameters and blades geometry for four paddle wheel blades were also proposed.
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In situ carbon supplementation in large-scale cultivations of Spirulina platensis in Open Raceway Pond
Biotechnology and Bioprocess Engineering, 2012Co-Authors: Yilu Bao, Wei Cong, Ming Liu, Zhengxiang NingAbstract:The objective of this study was to estimate the CO2 absorptivity provided by an in situ carbon supply system using a photosynthetic culture of the cyanobacterium Spirulina platensis in an Open Raceway Pond. The effects of initial total carbon concentrations (ranging from 0 to 0.1 mol/L), suspension depths (ranging from 5 to 20 cm) and pH values (ranging from 8.9 to 11.0) on the CO2 absorptivity were studied. The results indicated that CO2 absorptivity was positively correlated with pH value, negatively correlated with total carbon concentration, and only negligibly affected by the suspension depth. The optimum total carbon concentration range and pH range were 0.03 similar to 0.09 mol/L and 9.7 similar to 10.0, respectively. An average CO2 absorptivity of 86.16% and average CO2 utilization efficiency of 79.18% were achieved using this in situ carbon-supply system in large-scale cultivation of Spirulina platensis, with an initial total carbon concentration of 0.06 mol/L and pH value of 9.8. Our results demonstrated that this system could obtain a favorable CO2 utilization efficiency in outdoor, large-scale cultivation of Spirulina platensis in Open Raceway Ponds.
Xiaobin Wen - One of the best experts on this subject based on the ideXlab platform.
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Sequential phototrophic–mixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000 m 2 Open Raceway Pond
Biotechnology for biofuels, 2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia LiuAbstract:Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale Open Ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon. Laboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic–mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in Open Raceway Ponds. Graesiella sp. WBG-1 maximally produced 12.5 g biomass and 4.1 g lipids m−2 day−1 in SPMC in a 1000 m2 Raceway Pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97 × 105 CFU ml−1) is comparable to that of the phototrophic cultivations. SPMC is an effective and feasible method to cultivate lipid-rich microalgae in Open Raceway Ponds. Successful scale-up of SPMC in a commercial Raceway Pond (1000 m2 culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and β-carotene.
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MOESM3 of Sequential phototrophicâmixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000Â m2 Open Raceway Pond
2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia LiuAbstract:Additional file 3. Changes of residual nitrate and phosphate during the Graesiella cultivation in Open Raceway Pond
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Sequential phototrophic–mixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000 m2 Open Raceway Pond
BMC, 2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia LiuAbstract:Abstract Background Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale Open Ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon. Results Laboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic–mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in Open Raceway Ponds. Graesiella sp. WBG-1 maximally produced 12.5 g biomass and 4.1 g lipids m−2 day−1 in SPMC in a 1000 m2 Raceway Pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97 × 105 CFU ml−1) is comparable to that of the phototrophic cultivations. Conclusions SPMC is an effective and feasible method to cultivate lipid-rich microalgae in Open Raceway Ponds. Successful scale-up of SPMC in a commercial Raceway Pond (1000 m2 culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and β-carotene
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sequential phototrophic mixotrophic cultivation of oleaginous microalga graesiella sp wbg 1 in a 1000 m 2 Open Raceway Pond
Biotechnology for Biofuels, 2019Co-Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Lin Liang, Aoqi Zhang, Caixia Liu, Yahong GengAbstract:Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale Open Ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon. Laboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic–mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in Open Raceway Ponds. Graesiella sp. WBG-1 maximally produced 12.5 g biomass and 4.1 g lipids m−2 day−1 in SPMC in a 1000 m2 Raceway Pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97 × 105 CFU ml−1) is comparable to that of the phototrophic cultivations. SPMC is an effective and feasible method to cultivate lipid-rich microalgae in Open Raceway Ponds. Successful scale-up of SPMC in a commercial Raceway Pond (1000 m2 culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and β-carotene.
