The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform
Randall J. Cohrs - One of the best experts on this subject based on the ideXlab platform.
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Varicella-Zoster Virus Open Reading Frame 48 Encodes an Active Nuclease
Journal of virology, 2013Co-Authors: Niklaus H. Mueller, Donald H. Gilden, Randall J. CohrsAbstract:Based on a DNA sequence and relative genomic position similar to those other herpesviruses, varicella-zoster virus (VZV) Open Reading Frame 48 (ORF48) is predicted to encode an alkaline nuclease. Here we report the cloning, expression, purification, and characterization of recombinant VZV ORF48 protein and a VZV ORF48 point mutation (T172P). Protein encoded by wild-type ORF48, but not mutant protein, displayed both endo- and exonuclease activity, identifying ORF48 as a potential therapeutic target in VZV disease since efficient viral replication requires viral nuclease activity.
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Expression of protein encoded by varicella-zoster virus Open Reading Frame 63 in latently infected human ganglionic neurons.
Proceedings of the National Academy of Sciences of the United States of America, 1996Co-Authors: Ravy Mahalingam, Mary Wellish, Randall J. Cohrs, Serge Debrus, Jacques Piette, Bernard Rentier, Donald H. GildenAbstract:Abstract The ganglionic cell type in which varicella-zoster virus (VZV) is latent in humans was analyzed by using antibodies raised against in vitro-expressed VZV Open Reading Frame 63 protein. VZV Open Reading Frame 63 protein was detected exclusively in the cytoplasm of neurons of latently infected human trigeminal and thoracic ganglia. This is, to our knowledge, the first identification of a herpesvirus protein expressed during latency in the human nervous system.
Donald H. Gilden - One of the best experts on this subject based on the ideXlab platform.
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Varicella-Zoster Virus Open Reading Frame 48 Encodes an Active Nuclease
Journal of virology, 2013Co-Authors: Niklaus H. Mueller, Donald H. Gilden, Randall J. CohrsAbstract:Based on a DNA sequence and relative genomic position similar to those other herpesviruses, varicella-zoster virus (VZV) Open Reading Frame 48 (ORF48) is predicted to encode an alkaline nuclease. Here we report the cloning, expression, purification, and characterization of recombinant VZV ORF48 protein and a VZV ORF48 point mutation (T172P). Protein encoded by wild-type ORF48, but not mutant protein, displayed both endo- and exonuclease activity, identifying ORF48 as a potential therapeutic target in VZV disease since efficient viral replication requires viral nuclease activity.
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Expression of protein encoded by varicella-zoster virus Open Reading Frame 63 in latently infected human ganglionic neurons.
Proceedings of the National Academy of Sciences of the United States of America, 1996Co-Authors: Ravy Mahalingam, Mary Wellish, Randall J. Cohrs, Serge Debrus, Jacques Piette, Bernard Rentier, Donald H. GildenAbstract:Abstract The ganglionic cell type in which varicella-zoster virus (VZV) is latent in humans was analyzed by using antibodies raised against in vitro-expressed VZV Open Reading Frame 63 protein. VZV Open Reading Frame 63 protein was detected exclusively in the cytoplasm of neurons of latently infected human trigeminal and thoracic ganglia. This is, to our knowledge, the first identification of a herpesvirus protein expressed during latency in the human nervous system.
Paul R. Kinchington - One of the best experts on this subject based on the ideXlab platform.
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Anatomy of the varicella-zoster virus Open-Reading Frame 4 promoter
The Journal of Infectious Diseases, 1998Co-Authors: Eric J. Michael, Karen M. Kuck, Paul R. KinchingtonAbstract:The regulation of varicella-zoster virus (VZV) gene expression is largely controlled at the transcriptional level by a few key viral proteins cooperating with one another and with cellular transcription factors. However, the mechanisms involved are largely unclear. To identify the sequences important for the transcriptional regulation of Open-Reading Frame (ORF) 4, itself encoding a transcriptional regulator, a mutation analysis of the promoter was done. These studies identified an element between -69 and -59 (relative to the transcriptional start site), which was critical to the activity of the promoter upon stimulation by the VZV transactivator IE62 and by VZV infection. DNA-protein interaction studies revealed that VZV induced the binding of a specific protein complex to this element, which contained the ubiquitous transcription factor USF. ORF 4 is the second VZV gene (in addition to VZV ORF 29) in which USF binding plays a critical role in gene expression.
Abbas Vafai - One of the best experts on this subject based on the ideXlab platform.
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Stability of varicella-zoster virus Open Reading Frame 63
Archives of virology, 2008Co-Authors: Merry Liu, Nicholas Vafai, Angela Liu, John Hart, Hsi Liu, Xiaoling Tang, Dongxia Wang, Abbas VafaiAbstract:The stability of varicella-zoster virus (VZV) Open Reading Frame (ORF) 63 was analyzed by sequential passage of a virus strain in cell culture. VZV was propagated in culture for 1,206 passages. ORF63 from six passages (18, 220, 516, 730, 1060, and 1,206) was selected and sequenced. Among the six passages, only passage 1,206 showed point mutations at three locations: 551, 618 and 661. In addition, western blot analysis with anti-ORF63 monoclonal antibodies showed no discernable difference in the size of the ORF63 gene product from passage 18 and that from passage 1,206. These results indicate the stability of VZV ORF63 gene in culture over 1,206 passages.
Eric J. Michael - One of the best experts on this subject based on the ideXlab platform.
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Anatomy of the varicella-zoster virus Open-Reading Frame 4 promoter
The Journal of Infectious Diseases, 1998Co-Authors: Eric J. Michael, Karen M. Kuck, Paul R. KinchingtonAbstract:The regulation of varicella-zoster virus (VZV) gene expression is largely controlled at the transcriptional level by a few key viral proteins cooperating with one another and with cellular transcription factors. However, the mechanisms involved are largely unclear. To identify the sequences important for the transcriptional regulation of Open-Reading Frame (ORF) 4, itself encoding a transcriptional regulator, a mutation analysis of the promoter was done. These studies identified an element between -69 and -59 (relative to the transcriptional start site), which was critical to the activity of the promoter upon stimulation by the VZV transactivator IE62 and by VZV infection. DNA-protein interaction studies revealed that VZV induced the binding of a specific protein complex to this element, which contained the ubiquitous transcription factor USF. ORF 4 is the second VZV gene (in addition to VZV ORF 29) in which USF binding plays a critical role in gene expression.
