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Shu-fen Weng – One of the best experts on this subject based on the ideXlab platform.

  • Riboflavin Synthesis Genes ribE, ribB, ribH, ribA Reside in the lux Operon of Photobacterium leiognathi
    Biochemical and biophysical research communications, 2001
    Co-Authors: Juey-wen Lin, Yuh-fen Chao, Shu-fen Weng
    Abstract:

    Abstract Nucleotide sequence of the riboflavin synthesis genes ribE, ribB, ribH, ribA (GenBank Accession No. AF364106) resided in the lux Operon of Photobacterium leiognathi PL741 has been determined, and the amino acid sequences of riboflavin synthetase (RibE), DHBP synthetase (RibB), lumazine synthetase (RibH), GTP cyclohydrolase II (RibA) encoded by the riboflavin synthesis genes are deduced. Nucleotide sequence reveals that the ribE gene encodes the riboflavin synthetase responsible for converting lumazine to riboflavin, the ribB gene encodes the DHBP synthetase responsible for 3,4-dihydroxyl-2-butanone 4-phosphate synthesis, the ribH gene encodes the lumazine synthetase responsible for lumazine synthesis, and the ribA gene encodes the GTP cyclohydrolase II responsible for lumazine synthesis. Functional analysis illustrates that the specific segments lay behind the ribH and ribA genes might form potential loops ΩoT and ΩTI–ΩTII; ΩoT is functioned as mRNA stability loop or/and for subregulation by alternative modulation, and ΩTI–ΩTII could be the transcriptional terminator of the lux Operon. The gene order of the ribE, ribB, ribH, ribA genes resided in the lux Operon and linked to the lum Operon is ←tero-lumQ-lumP-RR ter: transcriptional terminator), whereas the R&R is the regulatory region for the lum and the lux Operons, and ter and tero are the transcriptional terminators for the lux and lum Operons.

Juey-wen Lin – One of the best experts on this subject based on the ideXlab platform.

  • Riboflavin Synthesis Genes ribE, ribB, ribH, ribA Reside in the lux Operon of Photobacterium leiognathi
    Biochemical and biophysical research communications, 2001
    Co-Authors: Juey-wen Lin, Yuh-fen Chao, Shu-fen Weng
    Abstract:

    Abstract Nucleotide sequence of the riboflavin synthesis genes ribE, ribB, ribH, ribA (GenBank Accession No. AF364106) resided in the lux Operon of Photobacterium leiognathi PL741 has been determined, and the amino acid sequences of riboflavin synthetase (RibE), DHBP synthetase (RibB), lumazine synthetase (RibH), GTP cyclohydrolase II (RibA) encoded by the riboflavin synthesis genes are deduced. Nucleotide sequence reveals that the ribE gene encodes the riboflavin synthetase responsible for converting lumazine to riboflavin, the ribB gene encodes the DHBP synthetase responsible for 3,4-dihydroxyl-2-butanone 4-phosphate synthesis, the ribH gene encodes the lumazine synthetase responsible for lumazine synthesis, and the ribA gene encodes the GTP cyclohydrolase II responsible for lumazine synthesis. Functional analysis illustrates that the specific segments lay behind the ribH and ribA genes might form potential loops ΩoT and ΩTI–ΩTII; ΩoT is functioned as mRNA stability loop or/and for subregulation by alternative modulation, and ΩTI–ΩTII could be the transcriptional terminator of the lux Operon. The gene order of the ribE, ribB, ribH, ribA genes resided in the lux Operon and linked to the lum Operon is ←tero-lumQ-lumP-RR ter: transcriptional terminator), whereas the R&R is the regulatory region for the lum and the lux Operons, and ter and tero are the transcriptional terminators for the lux and lum Operons.

Yuh-fen Chao – One of the best experts on this subject based on the ideXlab platform.

  • Riboflavin Synthesis Genes ribE, ribB, ribH, ribA Reside in the lux Operon of Photobacterium leiognathi
    Biochemical and biophysical research communications, 2001
    Co-Authors: Juey-wen Lin, Yuh-fen Chao, Shu-fen Weng
    Abstract:

    Abstract Nucleotide sequence of the riboflavin synthesis genes ribE, ribB, ribH, ribA (GenBank Accession No. AF364106) resided in the lux Operon of Photobacterium leiognathi PL741 has been determined, and the amino acid sequences of riboflavin synthetase (RibE), DHBP synthetase (RibB), lumazine synthetase (RibH), GTP cyclohydrolase II (RibA) encoded by the riboflavin synthesis genes are deduced. Nucleotide sequence reveals that the ribE gene encodes the riboflavin synthetase responsible for converting lumazine to riboflavin, the ribB gene encodes the DHBP synthetase responsible for 3,4-dihydroxyl-2-butanone 4-phosphate synthesis, the ribH gene encodes the lumazine synthetase responsible for lumazine synthesis, and the ribA gene encodes the GTP cyclohydrolase II responsible for lumazine synthesis. Functional analysis illustrates that the specific segments lay behind the ribH and ribA genes might form potential loops ΩoT and ΩTI–ΩTII; ΩoT is functioned as mRNA stability loop or/and for subregulation by alternative modulation, and ΩTI–ΩTII could be the transcriptional terminator of the lux Operon. The gene order of the ribE, ribB, ribH, ribA genes resided in the lux Operon and linked to the lum Operon is ←tero-lumQ-lumP-RR ter: transcriptional terminator), whereas the R&R is the regulatory region for the lum and the lux Operons, and ter and tero are the transcriptional terminators for the lux and lum Operons.

Weng S.f. – One of the best experts on this subject based on the ideXlab platform.

  • Riboflavin synthesis genes ribE, ribB, ribH, ribA reside in the lux Operon of Photobacterium leiognathi
    , 2014
    Co-Authors: Lin J.w., Chao Y.f., Weng S.f.
    Abstract:

    Nucleotide sequence of the riboflavin synthesis genes ribE, ribB, ribH, ribA (GenBank Accession No. AF364106) resided in the lux Operon of Photobacterium leiognathi PL741 has been determined, and the amino acid sequences of riboflavin synthetase (RibE), DHBP synthetase (RibB), lumazine synthetase (RibH), GTP cyclohydrolase II (RibA) encoded by the riboflavin synthesis genes are deduced. Nucleotide sequence reveals that the ribE gene encodes the riboflavin synthetase responsible for converting lumazine to riboflavin, the ribB gene encodes the DHBP synthetase responsible for 3,4-dihydroxyl-2-butanone 4-phosphate synthesis, the ribH gene encodes the lumazine synthetase responsible for lumazine synthesis, and the ribA gene encodes the GTP cyclohydrolase II responsible for lumazine synthesis. Functional analysis illustrates that the specific segments lay behind the ribH and ribA genes might form potential loops Omega (oT) and Omega (TI)-Omega (TII); Omega (oT) is functioned as mRNA stability loop or/and for subregulation by alternative modulation, and Omega (TI)-Omega (TII) could be the transcriptional terminator of the lux Operon. The gene order of the ribE, ribB, ribH, ribA genes resided in the lux Operon and linked to the lum Operon is , (R&R: regulatory region; ter: transcriptional terminator), whereas the R&R is the regulatory region for the lum and the lux Operons, and ter and ter(o) are the transcriptional terminators for the lux and lum Operons. (C) 2001 Academic Press

Gyorgy Posfai – One of the best experts on this subject based on the ideXlab platform.

  • engineered ribosomal rna Operon copy number variants of e coli reveal the evolutionary trade offs shaping rrna Operon number
    Nucleic Acids Research, 2015
    Co-Authors: Zsuzsanna Gyorfy, Gabor Draskovits, Viktor Vernyik, Frederick F Blattner, Tamas Gaal, Gyorgy Posfai
    Abstract:

    Ribosomal RNA (rrn) Operons, characteristically present in several copies in bacterial genomes (7 in E. coli), play a central role in cellular physiology. We investigated the factors determining the optimal number of rrn Operons in E. coli by constructing isogenic variants with 5–10 Operons. We found that the total RNA and protein content, as well as the size of the cells reflected the number of rrn Operons. While growth parameters showed only minor differences, competition experiments revealed a clear pattern: 7–8 copies were optimal under conditions of fluctuating, occasionally rich nutrient influx and lower numbers were favored in stable, nutrient-limited environments. We found that the advantages of quick adjustment to nutrient availability, rapid growth and economic regulation of ribosome number all contribute to the selection of the optimal rrn Operon number. Our results suggest that the wt rrn Operon number of E. coli reflects the natural, ‘feast and famine’ life-style of the bacterium, however, different copy numbers might be beneficial under different environmental conditions. Understanding the impact of the copy number of rrn Operons on the fitness of the cell is an important step towards the creation of functional and robust genomes, the ultimate goal of synthetic biology.