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Jun Yang - One of the best experts on this subject based on the ideXlab platform.

  • Serotonin Influences the Endogenous Opiate Peptides in the Rat Spinal Cord to Participates in Pain Modulation
    2016
    Co-Authors: Yan-juan Pan, Jun Yang, Zhi-kui Yin, Ying Zhao, Da-xin Wang
    Abstract:

    Spinal cord is a necessary pathway that transfers the body nociceptive inputs to the brain. Endogenous Opiate Peptides have been proven to participate in the nociceptive process at spinal level. It has reported that serotonin (5-HT, 5-hy-droxytryptamine) in spinal cord plays a role in pan modulation, which can be blocked by Opiate receptor antagonists. The present study was designed to investigate the interaction between 5-HT and endogenous Opiate Peptides at rat spi-nal level effecting on pain modulation. The results showed that 1) pain stimulation increased not only leucine-en-kephalin (L-Ek), β-endorphin (β-Ep) and dynorphin A1-13 (DynA1-13) concentrations but also 5-HT and 5-hydorxyin-doleace acid (5-HIAA, the 5-HT main metabolic product) concentrations in spinal cord significantly; 2) 5-HT could increase L-Ek, β-Ep and DynA1-13 concentrations in spinal cord in a dose-dependent manner, whereas cypotolamine (a 5-HT receptor antagonist) decreased L-Ek, β-Ep and DynA1-13 concentrations in spinal cord. The data suggested that 5-HT antinociceptive role might be involved in the endogenous Opiate Peptide system through 5-HT receptors at spinal level

  • the antinociceptive role of central arginine vasopressin is involved in the endogenous Opiate Peptide serotonin and acetylcholine systems
    World Journal of Neuroscience, 2011
    Co-Authors: Jun Yang, Yan-juan Pan, Ying Zhao, Xiqing Yan, Peiyong Qiu, Xijian Zhou, Da-xin Wang
    Abstract:

    Our previous work has demonstrated that arginine vasopressin (AVP) plays a role in pain modulation. The present study investigated which kinds of neuroPeptides and neurotransmitters in central nervous system might be involved in AVP antinociceptive role in the rat. The results showed that (1) intraventricular injection (icv) of V1 receptor antagonist [d(CH2)5Tyr(Me)AVP] and V2 receptor antagonist [d(CH2)5[D-Ile2, Ile4, Ala9-NH2]AVP] blocked the antinociceptive effect induced by AVP (icv), (2) the Opiate recaptor antagonist (naloxone) reversed the antinociceptive effect induced by AVP (icv), and (3) both the serotonin receptor antagonist (cypoheptadine) and M receptor antagonist (atropine) could attenuate the antinociceptive effect induced by AVP (icv); but (4) oxytocin, dopamine, N-methyl-D-aspartate (NMDA), γ-aminobutyric acid (GABA), N, α or β receptor antagonist did not influence the antinociceptive effect induced by AVP (icv). The data suggested that AVP antinociceptive role was involved in the endogenous Opiate Peptide, serotonin and acetylcholine systems in central nervous system.

  • arginine vasopressin antinociception in the rat nucleus raphe magnus is involved in the endogenous Opiate Peptide and serotonin system
    Peptides, 2009
    Co-Authors: Jun Yang, Wen-yan Liu, Gen Wang, Huifeng Yuan, Jiegen Chu, Yu Yang, Bao-cheng Lin
    Abstract:

    Abstract Arginine vasopressin (AVP) in the nucleus raphe magnus (NRM) has been implicated in antinociception. This communication was designed to investigate which neuroPeptide and neurotransmitter are involved in AVP antinociception in the rat NRM. The results showed that (1) in the NRM perfuse liquid, pain stimulation could increase the concentrations of AVP, leucine-enkephalin (L-Ek), methionine-enkephalin (M-Ek), β-endorphin (β-Ep), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), but not change the concentrations of dynorphinA1–13 (DynA1–13), oxytocin, achetylcholine, choline, γ-aminobutyric acid, glutamate, dopamine, 3,4-dihydroxyphenylacetic acid, homovanilic acid, norepinephrine and epinephrine; (2) in the NRM perfuse liquid, AVP increased the concentrations of L-Ek, M-Ek, β-Ep, DynA1–13, 5-HT and 5-HIAA, but did not change the concentrations of oxytocin and the other studied neurotransmitters; (3) AVP antinociception in the NRM was attenuated by cypoheptadine (a 5-HT-receptor antagonist) or naloxone (an Opiate receptor antagonist), but was not influenced by the other studied receptor antagonists. The data suggested that AVP antinociception in the NRM might be involved in endogenous Opiate Peptide and 5-HT system.

  • through the central v2 not v1 receptors influencing the endogenous Opiate Peptide system arginine vasopressin not oxytocin in the hypothalamic paraventricular nucleus involves in the antinociception in the rat
    Brain Research, 2006
    Co-Authors: Jun Yang, Jian-min Chen, Wen-yan Liu, Cao-you Song, Gen Wang, Chenghai Wang, Bao-chen Lin
    Abstract:

    Our previous study has proven that hypothalamic paraventricular nucleus (PVN) played a role in the antinociception. The central bioactive substances involving in the PVN regulating antinociception were investigated in the rat. The results showed that electrical stimulation of the PVN increased the pain threshold, and L-glutamate sodium injection into the PVN elevated the pain threshold, but the PVN cauterization decreased the pain threshold; pain stimulation raised the arginine vasopressin (AVP), not oxytocin (OXT), leucine-enkephalin (L-Ek), beta-endorphin (beta-Ep) and DynorphinA1-13 (DynA1-13) concentrations in the PVN tissue using micropunch method, heightened AVP, L-Ek, beta-Ep and DynA1-13, not OXT concentrations in the PVN perfuse liquid, and reduced the number of AVP-, not OXT, L-Ek, beta-Ep and DynA1-13-immunoreactive neurons in the PVN especially in the posterior magnocellular part of the PVN using immunocytochemistry. There was a negative relationship between the PVN AVP concentration and the pain threshold; pain stimulation enhanced the AVP, not OXT mRNA expression in the PVN using in situ hybridization and RT-PCR; intraventricular injection of anti-AVP serum completely reversed L-glutamate sodium injection into the PVN-induced antinociception, and administration of naloxone - the Opiate Peptide antagonist, partly blocked this L-glutamate sodium effect, but anti-OXT serum pretreatment did not influence this L-glutamate sodium effect; L-glutamate sodium injection into the PVN-induced analgesia was inhibited by V2 receptor antagonist - d(CH2)5[D-Ile2, Ile4, Ala-NH2(9)]AVP, not V1 receptor antagonist - d(CH2)5Tyr(Me)AVP. The data suggested that the PVN was limited to the central AVP, not OXT, which was through V2, not V1 receptors influencing the endogenous Opiate Peptide system, to regulate antinociception.

  • Through central arginine vasopressin, not oxytocin and endogenous Opiate Peptides, glutamate sodium induces hypothalamic paraventricular nucleus enhancing acupuncture analgesia in the rat.
    Neuroscience research, 2005
    Co-Authors: Jun Yang, Wen-yan Liu, Cao-you Song, Bao-cheng Lin
    Abstract:

    Abstract Our previous study proved that the hypothalamic paraventricular nucleus (PVH) plays an important role in acupuncture analgesia. The neuroPeptides involving in the PVH regulation of acupuncture analgesia was investigated in the rat. The changes of pain threshold, which was induced by electrical acupuncture of “Zusanli” points (St. 36), were measured as acupuncture analgesia. Microinjection of l -glutamate sodium into the PVH, which only excites the PVH neurons, could dose-dependently enhance the acupuncture analgesia, but microinjection of l -glutamate sodium into the area nearby the PVH did not alter acupuncture analgesia. Removing pituitary did not influence this effect of l -glutamate sodium. Microinjection of l -glutamate sodium into the PVH only increased the arginine vasopressin (AVP), not oxytocin (OXT), leucine enkephaline (L-Ek), β-endorphine (β-Ep) and dynorphinA 1–13 (DynA 1–13 ) concentrations in the PVH perfuse liquid using radioimmunoassay. Intraventricular injection of anti-arginine vasopressin serum (AAVPS) could completely reverse the effect of microinjection of l -glutamate sodium into the PVH enhancing acupuncture analgesia. Intraventricular injection of naloxone, one Opiate Peptide antagonist, partly attenuated this effect of l -glutamate sodium, and intraventricular of anti-oxytocin serum (AOXTS) did not change this effect of l -glutamate sodium. The results suggested that l -glutamate sodium induces the PVH enhancing acupuncture analgesia only through AVP, not OXT and endogenous Opiate Peptides in central nervous system.

Bao-cheng Lin - One of the best experts on this subject based on the ideXlab platform.

  • arginine vasopressin antinociception in the rat nucleus raphe magnus is involved in the endogenous Opiate Peptide and serotonin system
    Peptides, 2009
    Co-Authors: Jun Yang, Wen-yan Liu, Gen Wang, Huifeng Yuan, Jiegen Chu, Yu Yang, Bao-cheng Lin
    Abstract:

    Abstract Arginine vasopressin (AVP) in the nucleus raphe magnus (NRM) has been implicated in antinociception. This communication was designed to investigate which neuroPeptide and neurotransmitter are involved in AVP antinociception in the rat NRM. The results showed that (1) in the NRM perfuse liquid, pain stimulation could increase the concentrations of AVP, leucine-enkephalin (L-Ek), methionine-enkephalin (M-Ek), β-endorphin (β-Ep), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), but not change the concentrations of dynorphinA1–13 (DynA1–13), oxytocin, achetylcholine, choline, γ-aminobutyric acid, glutamate, dopamine, 3,4-dihydroxyphenylacetic acid, homovanilic acid, norepinephrine and epinephrine; (2) in the NRM perfuse liquid, AVP increased the concentrations of L-Ek, M-Ek, β-Ep, DynA1–13, 5-HT and 5-HIAA, but did not change the concentrations of oxytocin and the other studied neurotransmitters; (3) AVP antinociception in the NRM was attenuated by cypoheptadine (a 5-HT-receptor antagonist) or naloxone (an Opiate receptor antagonist), but was not influenced by the other studied receptor antagonists. The data suggested that AVP antinociception in the NRM might be involved in endogenous Opiate Peptide and 5-HT system.

  • Through central arginine vasopressin, not oxytocin and endogenous Opiate Peptides, glutamate sodium induces hypothalamic paraventricular nucleus enhancing acupuncture analgesia in the rat.
    Neuroscience research, 2005
    Co-Authors: Jun Yang, Wen-yan Liu, Cao-you Song, Bao-cheng Lin
    Abstract:

    Abstract Our previous study proved that the hypothalamic paraventricular nucleus (PVH) plays an important role in acupuncture analgesia. The neuroPeptides involving in the PVH regulation of acupuncture analgesia was investigated in the rat. The changes of pain threshold, which was induced by electrical acupuncture of “Zusanli” points (St. 36), were measured as acupuncture analgesia. Microinjection of l -glutamate sodium into the PVH, which only excites the PVH neurons, could dose-dependently enhance the acupuncture analgesia, but microinjection of l -glutamate sodium into the area nearby the PVH did not alter acupuncture analgesia. Removing pituitary did not influence this effect of l -glutamate sodium. Microinjection of l -glutamate sodium into the PVH only increased the arginine vasopressin (AVP), not oxytocin (OXT), leucine enkephaline (L-Ek), β-endorphine (β-Ep) and dynorphinA 1–13 (DynA 1–13 ) concentrations in the PVH perfuse liquid using radioimmunoassay. Intraventricular injection of anti-arginine vasopressin serum (AAVPS) could completely reverse the effect of microinjection of l -glutamate sodium into the PVH enhancing acupuncture analgesia. Intraventricular injection of naloxone, one Opiate Peptide antagonist, partly attenuated this effect of l -glutamate sodium, and intraventricular of anti-oxytocin serum (AOXTS) did not change this effect of l -glutamate sodium. The results suggested that l -glutamate sodium induces the PVH enhancing acupuncture analgesia only through AVP, not OXT and endogenous Opiate Peptides in central nervous system.

Wen-yan Liu - One of the best experts on this subject based on the ideXlab platform.

  • arginine vasopressin antinociception in the rat nucleus raphe magnus is involved in the endogenous Opiate Peptide and serotonin system
    Peptides, 2009
    Co-Authors: Jun Yang, Wen-yan Liu, Gen Wang, Huifeng Yuan, Jiegen Chu, Yu Yang, Bao-cheng Lin
    Abstract:

    Abstract Arginine vasopressin (AVP) in the nucleus raphe magnus (NRM) has been implicated in antinociception. This communication was designed to investigate which neuroPeptide and neurotransmitter are involved in AVP antinociception in the rat NRM. The results showed that (1) in the NRM perfuse liquid, pain stimulation could increase the concentrations of AVP, leucine-enkephalin (L-Ek), methionine-enkephalin (M-Ek), β-endorphin (β-Ep), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), but not change the concentrations of dynorphinA1–13 (DynA1–13), oxytocin, achetylcholine, choline, γ-aminobutyric acid, glutamate, dopamine, 3,4-dihydroxyphenylacetic acid, homovanilic acid, norepinephrine and epinephrine; (2) in the NRM perfuse liquid, AVP increased the concentrations of L-Ek, M-Ek, β-Ep, DynA1–13, 5-HT and 5-HIAA, but did not change the concentrations of oxytocin and the other studied neurotransmitters; (3) AVP antinociception in the NRM was attenuated by cypoheptadine (a 5-HT-receptor antagonist) or naloxone (an Opiate receptor antagonist), but was not influenced by the other studied receptor antagonists. The data suggested that AVP antinociception in the NRM might be involved in endogenous Opiate Peptide and 5-HT system.

  • through the central v2 not v1 receptors influencing the endogenous Opiate Peptide system arginine vasopressin not oxytocin in the hypothalamic paraventricular nucleus involves in the antinociception in the rat
    Brain Research, 2006
    Co-Authors: Jun Yang, Jian-min Chen, Wen-yan Liu, Cao-you Song, Gen Wang, Chenghai Wang, Bao-chen Lin
    Abstract:

    Our previous study has proven that hypothalamic paraventricular nucleus (PVN) played a role in the antinociception. The central bioactive substances involving in the PVN regulating antinociception were investigated in the rat. The results showed that electrical stimulation of the PVN increased the pain threshold, and L-glutamate sodium injection into the PVN elevated the pain threshold, but the PVN cauterization decreased the pain threshold; pain stimulation raised the arginine vasopressin (AVP), not oxytocin (OXT), leucine-enkephalin (L-Ek), beta-endorphin (beta-Ep) and DynorphinA1-13 (DynA1-13) concentrations in the PVN tissue using micropunch method, heightened AVP, L-Ek, beta-Ep and DynA1-13, not OXT concentrations in the PVN perfuse liquid, and reduced the number of AVP-, not OXT, L-Ek, beta-Ep and DynA1-13-immunoreactive neurons in the PVN especially in the posterior magnocellular part of the PVN using immunocytochemistry. There was a negative relationship between the PVN AVP concentration and the pain threshold; pain stimulation enhanced the AVP, not OXT mRNA expression in the PVN using in situ hybridization and RT-PCR; intraventricular injection of anti-AVP serum completely reversed L-glutamate sodium injection into the PVN-induced antinociception, and administration of naloxone - the Opiate Peptide antagonist, partly blocked this L-glutamate sodium effect, but anti-OXT serum pretreatment did not influence this L-glutamate sodium effect; L-glutamate sodium injection into the PVN-induced analgesia was inhibited by V2 receptor antagonist - d(CH2)5[D-Ile2, Ile4, Ala-NH2(9)]AVP, not V1 receptor antagonist - d(CH2)5Tyr(Me)AVP. The data suggested that the PVN was limited to the central AVP, not OXT, which was through V2, not V1 receptors influencing the endogenous Opiate Peptide system, to regulate antinociception.

  • Through central arginine vasopressin, not oxytocin and endogenous Opiate Peptides, glutamate sodium induces hypothalamic paraventricular nucleus enhancing acupuncture analgesia in the rat.
    Neuroscience research, 2005
    Co-Authors: Jun Yang, Wen-yan Liu, Cao-you Song, Bao-cheng Lin
    Abstract:

    Abstract Our previous study proved that the hypothalamic paraventricular nucleus (PVH) plays an important role in acupuncture analgesia. The neuroPeptides involving in the PVH regulation of acupuncture analgesia was investigated in the rat. The changes of pain threshold, which was induced by electrical acupuncture of “Zusanli” points (St. 36), were measured as acupuncture analgesia. Microinjection of l -glutamate sodium into the PVH, which only excites the PVH neurons, could dose-dependently enhance the acupuncture analgesia, but microinjection of l -glutamate sodium into the area nearby the PVH did not alter acupuncture analgesia. Removing pituitary did not influence this effect of l -glutamate sodium. Microinjection of l -glutamate sodium into the PVH only increased the arginine vasopressin (AVP), not oxytocin (OXT), leucine enkephaline (L-Ek), β-endorphine (β-Ep) and dynorphinA 1–13 (DynA 1–13 ) concentrations in the PVH perfuse liquid using radioimmunoassay. Intraventricular injection of anti-arginine vasopressin serum (AAVPS) could completely reverse the effect of microinjection of l -glutamate sodium into the PVH enhancing acupuncture analgesia. Intraventricular injection of naloxone, one Opiate Peptide antagonist, partly attenuated this effect of l -glutamate sodium, and intraventricular of anti-oxytocin serum (AOXTS) did not change this effect of l -glutamate sodium. The results suggested that l -glutamate sodium induces the PVH enhancing acupuncture analgesia only through AVP, not OXT and endogenous Opiate Peptides in central nervous system.

Yu Yang - One of the best experts on this subject based on the ideXlab platform.

  • arginine vasopressin antinociception in the rat nucleus raphe magnus is involved in the endogenous Opiate Peptide and serotonin system
    Peptides, 2009
    Co-Authors: Jun Yang, Wen-yan Liu, Gen Wang, Huifeng Yuan, Jiegen Chu, Yu Yang, Bao-cheng Lin
    Abstract:

    Abstract Arginine vasopressin (AVP) in the nucleus raphe magnus (NRM) has been implicated in antinociception. This communication was designed to investigate which neuroPeptide and neurotransmitter are involved in AVP antinociception in the rat NRM. The results showed that (1) in the NRM perfuse liquid, pain stimulation could increase the concentrations of AVP, leucine-enkephalin (L-Ek), methionine-enkephalin (M-Ek), β-endorphin (β-Ep), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), but not change the concentrations of dynorphinA1–13 (DynA1–13), oxytocin, achetylcholine, choline, γ-aminobutyric acid, glutamate, dopamine, 3,4-dihydroxyphenylacetic acid, homovanilic acid, norepinephrine and epinephrine; (2) in the NRM perfuse liquid, AVP increased the concentrations of L-Ek, M-Ek, β-Ep, DynA1–13, 5-HT and 5-HIAA, but did not change the concentrations of oxytocin and the other studied neurotransmitters; (3) AVP antinociception in the NRM was attenuated by cypoheptadine (a 5-HT-receptor antagonist) or naloxone (an Opiate receptor antagonist), but was not influenced by the other studied receptor antagonists. The data suggested that AVP antinociception in the NRM might be involved in endogenous Opiate Peptide and 5-HT system.

  • Arginine vasopressin antinociception in the rat nucleus raphe magnus is involved in the endogenous Opiate Peptide and serotonin system
    Elsevier, 2009
    Co-Authors: Yang Jun, Yu Yang, Yuan Huifeng, Chu Jiegen, Xu Hongtao, Wang Gen, Liu Wen-yan, Lin Bao-cheng
    Abstract:

    Arginine vasopressin (AVP) in the nucleus raphe magnus (NRM) has been implicated in antinociception. This communication was designed to investigate which neuroPeptide and neurotransmitter are involved in AVP antinociception in the rat NRM. The results showed that (1) in the NRM perfuse liquid, pain stimulation could increase the concentrations of AVP, leucine-enkephalin (L-Ek), methionine-enkephalin (M-Ek), \u3b2-endorphin (\u3b2-Ep), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), but not change the concentrations of dynorphinA1\u201313 (DynA1\u201313), oxytocin, achetylcholine, choline, \u3b3-aminobutyric acid, glutamate, dopamine, 3,4-dihydroxyphenylacetic acid, homovanilic acid, norepinephrine and epinephrine; (2) in the NRM perfuse liquid, AVP increased the concentrations of L-Ek, M-Ek, \u3b2-Ep, DynA1\u201313, 5-HT and 5-HIAA, but did not change the concentrations of oxytocin and the other studied neurotransmitters; (3) AVP antinociception in the NRM was attenuated by cypoheptadine (a 5-HT-receptor antagonist) or naloxone (an Opiate receptor antagonist), but was not influenced by the other studied receptor antagonists. The data suggested that AVP antinociception in the NRM might be involved in endogenous Opiate Peptide and 5-HT system.Peer reviewed: YesNRC publication: Ye

  • Endogenous Opiate Peptides in the spinal cord are involved in the analgesia of hypothalamic paraventricular nucleus in the rat
    Elsevier, 2009
    Co-Authors: Yang Jun, Yu Yang, Chu Jiegen, Xu Hongtao, Wang Gen, Liu Wen-yan, Wang Cheng-hai, Lin Bao-cheng
    Abstract:

    Many studies have shown that hypothalamic paraventricular nucleus (PVN) plays a role in pain process, and endogenous Opiate Peptide system in the spinal cord is involved in nociception. This communication was designed to study the relationship between PVN and endogenous Opiate system in the spinal cord in the rat. The results showed that in both the thoracic and the lumber spinal cord, microinjection of 100 ng l-glutamate sodium into PVN could increase leucine-enkephalin (L-Ek), \u3b2-endorphin (\u3b2-Ep), dynorphinA1\u201313 (DynA1\u201313) concentrations and PVN cauterization decreased L-Ek and \u3b2-Ep concentrations. Pretreatment of the spinal cord with 5 \u3bcg naloxone, an Opiate receptor antagonist could partly reverse the analgesia induced by microinjection of 100 ng l-glutamate sodium into PVN. The data suggested that PVN analgesia might be involved in the endogenous Opiate Peptide system in the spinal cord independently.Peer reviewed: YesNRC publication: Ye

  • Effect of arginine vasopressin on acupuncture analgesia in the rat
    Elsevier, 2008
    Co-Authors: Yang Jun, Yu Yang, Xu Hongtao, Wang Gen, Liu Wen-yan, Wang Cheng-hai, Lin Bao-cheng
    Abstract:

    Arginine vasopressin (AVP) has been proven to be involved in the process of pain regulation. This communication was designed to investigate the effect of AVP on acupuncture analgesia in the rat model. The results showed that intraventricular injection (icv) of AVP could enhance acupuncture analgesia in a dose-dependent manner, whereas icv of anti-AVP serum decreased acupuncture analgesia. However, neither intrathecal (ith) nor intravenous injection (iv) of AVP or anti-AVP serum could influence acupuncture analgesia. Electrical acupuncture of \u201cZusanli\u201d points (St. 36) decreased AVP concentration in the hypothalamic paraventricular nucleus (PVN), and increased AVP concentration in the hypothalamic supraoptic nucleus (SON), periaqueductial gray (PAG), caudate nucleus (CdN) and raphe magnus nucleus (RMN), but did not change AVP concentration in the pituitary, spinal cord and plasma. The effect of AVP on acupuncture analgesia was partly reversed by pretreatment with naloxone, an Opiate receptor antagonist. These data suggested that AVP in the brain played a role in the process of acupuncture analgesia in combination with the endogenous Opiate Peptide system.Peer reviewed: YesNRC publication: Ye

Gen Wang - One of the best experts on this subject based on the ideXlab platform.

  • arginine vasopressin antinociception in the rat nucleus raphe magnus is involved in the endogenous Opiate Peptide and serotonin system
    Peptides, 2009
    Co-Authors: Jun Yang, Wen-yan Liu, Gen Wang, Huifeng Yuan, Jiegen Chu, Yu Yang, Bao-cheng Lin
    Abstract:

    Abstract Arginine vasopressin (AVP) in the nucleus raphe magnus (NRM) has been implicated in antinociception. This communication was designed to investigate which neuroPeptide and neurotransmitter are involved in AVP antinociception in the rat NRM. The results showed that (1) in the NRM perfuse liquid, pain stimulation could increase the concentrations of AVP, leucine-enkephalin (L-Ek), methionine-enkephalin (M-Ek), β-endorphin (β-Ep), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA), but not change the concentrations of dynorphinA1–13 (DynA1–13), oxytocin, achetylcholine, choline, γ-aminobutyric acid, glutamate, dopamine, 3,4-dihydroxyphenylacetic acid, homovanilic acid, norepinephrine and epinephrine; (2) in the NRM perfuse liquid, AVP increased the concentrations of L-Ek, M-Ek, β-Ep, DynA1–13, 5-HT and 5-HIAA, but did not change the concentrations of oxytocin and the other studied neurotransmitters; (3) AVP antinociception in the NRM was attenuated by cypoheptadine (a 5-HT-receptor antagonist) or naloxone (an Opiate receptor antagonist), but was not influenced by the other studied receptor antagonists. The data suggested that AVP antinociception in the NRM might be involved in endogenous Opiate Peptide and 5-HT system.

  • through the central v2 not v1 receptors influencing the endogenous Opiate Peptide system arginine vasopressin not oxytocin in the hypothalamic paraventricular nucleus involves in the antinociception in the rat
    Brain Research, 2006
    Co-Authors: Jun Yang, Jian-min Chen, Wen-yan Liu, Cao-you Song, Gen Wang, Chenghai Wang, Bao-chen Lin
    Abstract:

    Our previous study has proven that hypothalamic paraventricular nucleus (PVN) played a role in the antinociception. The central bioactive substances involving in the PVN regulating antinociception were investigated in the rat. The results showed that electrical stimulation of the PVN increased the pain threshold, and L-glutamate sodium injection into the PVN elevated the pain threshold, but the PVN cauterization decreased the pain threshold; pain stimulation raised the arginine vasopressin (AVP), not oxytocin (OXT), leucine-enkephalin (L-Ek), beta-endorphin (beta-Ep) and DynorphinA1-13 (DynA1-13) concentrations in the PVN tissue using micropunch method, heightened AVP, L-Ek, beta-Ep and DynA1-13, not OXT concentrations in the PVN perfuse liquid, and reduced the number of AVP-, not OXT, L-Ek, beta-Ep and DynA1-13-immunoreactive neurons in the PVN especially in the posterior magnocellular part of the PVN using immunocytochemistry. There was a negative relationship between the PVN AVP concentration and the pain threshold; pain stimulation enhanced the AVP, not OXT mRNA expression in the PVN using in situ hybridization and RT-PCR; intraventricular injection of anti-AVP serum completely reversed L-glutamate sodium injection into the PVN-induced antinociception, and administration of naloxone - the Opiate Peptide antagonist, partly blocked this L-glutamate sodium effect, but anti-OXT serum pretreatment did not influence this L-glutamate sodium effect; L-glutamate sodium injection into the PVN-induced analgesia was inhibited by V2 receptor antagonist - d(CH2)5[D-Ile2, Ile4, Ala-NH2(9)]AVP, not V1 receptor antagonist - d(CH2)5Tyr(Me)AVP. The data suggested that the PVN was limited to the central AVP, not OXT, which was through V2, not V1 receptors influencing the endogenous Opiate Peptide system, to regulate antinociception.