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Ralph G Nuzzo - One of the best experts on this subject based on the ideXlab platform.

  • quantitative reflection Imaging of fixed aplysia californica pedal ganglion neurons on nanostructured plasmonic crystals
    Journal of Physical Chemistry B, 2013
    Co-Authors: Somi Kang, Lucas B Thompson, Stanislav S Rubakhin, Jonathan V Sweedler, John A Rogers, Ralph G Nuzzo
    Abstract:

    Studies of the interactions between cells and surrounding environment including cell culture surfaces and their responses to distinct chemical and physical cues are essential to understanding the regulation of cell growth, migration, and differentiation. In this work, we demonstrate the capability of a label-free Optical Imaging Technique—surface plasmon resonance (SPR)—to quantitatively investigate the relative thickness of complex biomolecular structures using a nanoimprinted plasmonic crystal and laboratory microscope. Polyelectrolyte films of different thicknesses deposited by layer-by-layer assembly served as the model system to calibrate the reflection contrast response originating from SPRs. The calibrated SPR system allows quantitative analysis of the thicknesses of the interface formed between the cell culture substrate and cellular membrane regions of fixed Aplysia californica pedal ganglion neurons. Bandpass filters were used to isolate spectral regions of reflected light with distinctive image...

  • quantitative reflection Imaging of fixed aplysia californica pedal ganglion neurons on nanostructured plasmonic crystals
    Journal of Physical Chemistry B, 2013
    Co-Authors: Somi Kang, Lucas B Thompson, Stanislav S Rubakhin, Jonathan V Sweedler, John A Rogers, Ralph G Nuzzo
    Abstract:

    Studies of the interactions between cells and surrounding environment including cell culture surfaces and their responses to distinct chemical and physical cues are essential to understanding the regulation of cell growth, migration, and differentiation. In this work, we demonstrate the capability of a label-free Optical Imaging Technique-surface plasmon resonance (SPR)-to quantitatively investigate the relative thickness of complex biomolecular structures using a nanoimprinted plasmonic crystal and laboratory microscope. Polyelectrolyte films of different thicknesses deposited by layer-by-layer assembly served as the model system to calibrate the reflection contrast response originating from SPRs. The calibrated SPR system allows quantitative analysis of the thicknesses of the interface formed between the cell culture substrate and cellular membrane regions of fixed Aplysia californica pedal ganglion neurons. Bandpass filters were used to isolate spectral regions of reflected light with distinctive image contrast changes. Combining of the data from images acquired using different bandpass filters leads to increase image contrast and sensitivity to topological differences in interface thicknesses. This SPR-based Imaging Technique is restricted in measurable thickness range (∼100-200 nm) due to the limited plasmonic sensing volume, but we complement this Technique with an interferometric analysis method. Described here simple reflection Imaging Techniques show promise as quantitative methods for analyzing surface thicknesses at nanometer scale over large areas in real-time and in physicochemical diverse environments.

Somi Kang - One of the best experts on this subject based on the ideXlab platform.

  • quantitative reflection Imaging of fixed aplysia californica pedal ganglion neurons on nanostructured plasmonic crystals
    Journal of Physical Chemistry B, 2013
    Co-Authors: Somi Kang, Lucas B Thompson, Stanislav S Rubakhin, Jonathan V Sweedler, John A Rogers, Ralph G Nuzzo
    Abstract:

    Studies of the interactions between cells and surrounding environment including cell culture surfaces and their responses to distinct chemical and physical cues are essential to understanding the regulation of cell growth, migration, and differentiation. In this work, we demonstrate the capability of a label-free Optical Imaging Technique—surface plasmon resonance (SPR)—to quantitatively investigate the relative thickness of complex biomolecular structures using a nanoimprinted plasmonic crystal and laboratory microscope. Polyelectrolyte films of different thicknesses deposited by layer-by-layer assembly served as the model system to calibrate the reflection contrast response originating from SPRs. The calibrated SPR system allows quantitative analysis of the thicknesses of the interface formed between the cell culture substrate and cellular membrane regions of fixed Aplysia californica pedal ganglion neurons. Bandpass filters were used to isolate spectral regions of reflected light with distinctive image...

  • quantitative reflection Imaging of fixed aplysia californica pedal ganglion neurons on nanostructured plasmonic crystals
    Journal of Physical Chemistry B, 2013
    Co-Authors: Somi Kang, Lucas B Thompson, Stanislav S Rubakhin, Jonathan V Sweedler, John A Rogers, Ralph G Nuzzo
    Abstract:

    Studies of the interactions between cells and surrounding environment including cell culture surfaces and their responses to distinct chemical and physical cues are essential to understanding the regulation of cell growth, migration, and differentiation. In this work, we demonstrate the capability of a label-free Optical Imaging Technique-surface plasmon resonance (SPR)-to quantitatively investigate the relative thickness of complex biomolecular structures using a nanoimprinted plasmonic crystal and laboratory microscope. Polyelectrolyte films of different thicknesses deposited by layer-by-layer assembly served as the model system to calibrate the reflection contrast response originating from SPRs. The calibrated SPR system allows quantitative analysis of the thicknesses of the interface formed between the cell culture substrate and cellular membrane regions of fixed Aplysia californica pedal ganglion neurons. Bandpass filters were used to isolate spectral regions of reflected light with distinctive image contrast changes. Combining of the data from images acquired using different bandpass filters leads to increase image contrast and sensitivity to topological differences in interface thicknesses. This SPR-based Imaging Technique is restricted in measurable thickness range (∼100-200 nm) due to the limited plasmonic sensing volume, but we complement this Technique with an interferometric analysis method. Described here simple reflection Imaging Techniques show promise as quantitative methods for analyzing surface thicknesses at nanometer scale over large areas in real-time and in physicochemical diverse environments.

Oleg D Lavrentovich - One of the best experts on this subject based on the ideXlab platform.

  • three dimensional Imaging of chemical bond orientation in liquid crystals by coherent anti stokes raman scattering microscopy
    Optics Express, 2007
    Co-Authors: Brian G Saar, Heungshik Park, Sunney X Xie, Oleg D Lavrentovich
    Abstract:

    Liquid crystals are a class of industrially important materials whose Optical properties make them useful particularly in display technology. Optical Imaging of these materials provides information about their structure and physical properties. Coherent anti-Stokes Raman scattering (CARS) microscopy is used to provide three-dimensional chemical maps of liquid crystalline samples without the use of external labels. CARS is an Optical Imaging Technique that derives contrast from Raman-active molecular vibrations in the sample. Compared to many other three-dimensional Imaging Techniques, CARS offers more rapid chemical characterization without the use of external dyes or contrast agents. The use of CARS to image chemical and orientational order in liquid crystals is demonstrated using several examples, and the limitations and benefits are discussed.

Lucas B Thompson - One of the best experts on this subject based on the ideXlab platform.

  • quantitative reflection Imaging of fixed aplysia californica pedal ganglion neurons on nanostructured plasmonic crystals
    Journal of Physical Chemistry B, 2013
    Co-Authors: Somi Kang, Lucas B Thompson, Stanislav S Rubakhin, Jonathan V Sweedler, John A Rogers, Ralph G Nuzzo
    Abstract:

    Studies of the interactions between cells and surrounding environment including cell culture surfaces and their responses to distinct chemical and physical cues are essential to understanding the regulation of cell growth, migration, and differentiation. In this work, we demonstrate the capability of a label-free Optical Imaging Technique—surface plasmon resonance (SPR)—to quantitatively investigate the relative thickness of complex biomolecular structures using a nanoimprinted plasmonic crystal and laboratory microscope. Polyelectrolyte films of different thicknesses deposited by layer-by-layer assembly served as the model system to calibrate the reflection contrast response originating from SPRs. The calibrated SPR system allows quantitative analysis of the thicknesses of the interface formed between the cell culture substrate and cellular membrane regions of fixed Aplysia californica pedal ganglion neurons. Bandpass filters were used to isolate spectral regions of reflected light with distinctive image...

  • quantitative reflection Imaging of fixed aplysia californica pedal ganglion neurons on nanostructured plasmonic crystals
    Journal of Physical Chemistry B, 2013
    Co-Authors: Somi Kang, Lucas B Thompson, Stanislav S Rubakhin, Jonathan V Sweedler, John A Rogers, Ralph G Nuzzo
    Abstract:

    Studies of the interactions between cells and surrounding environment including cell culture surfaces and their responses to distinct chemical and physical cues are essential to understanding the regulation of cell growth, migration, and differentiation. In this work, we demonstrate the capability of a label-free Optical Imaging Technique-surface plasmon resonance (SPR)-to quantitatively investigate the relative thickness of complex biomolecular structures using a nanoimprinted plasmonic crystal and laboratory microscope. Polyelectrolyte films of different thicknesses deposited by layer-by-layer assembly served as the model system to calibrate the reflection contrast response originating from SPRs. The calibrated SPR system allows quantitative analysis of the thicknesses of the interface formed between the cell culture substrate and cellular membrane regions of fixed Aplysia californica pedal ganglion neurons. Bandpass filters were used to isolate spectral regions of reflected light with distinctive image contrast changes. Combining of the data from images acquired using different bandpass filters leads to increase image contrast and sensitivity to topological differences in interface thicknesses. This SPR-based Imaging Technique is restricted in measurable thickness range (∼100-200 nm) due to the limited plasmonic sensing volume, but we complement this Technique with an interferometric analysis method. Described here simple reflection Imaging Techniques show promise as quantitative methods for analyzing surface thicknesses at nanometer scale over large areas in real-time and in physicochemical diverse environments.

Stanislav S Rubakhin - One of the best experts on this subject based on the ideXlab platform.

  • quantitative reflection Imaging of fixed aplysia californica pedal ganglion neurons on nanostructured plasmonic crystals
    Journal of Physical Chemistry B, 2013
    Co-Authors: Somi Kang, Lucas B Thompson, Stanislav S Rubakhin, Jonathan V Sweedler, John A Rogers, Ralph G Nuzzo
    Abstract:

    Studies of the interactions between cells and surrounding environment including cell culture surfaces and their responses to distinct chemical and physical cues are essential to understanding the regulation of cell growth, migration, and differentiation. In this work, we demonstrate the capability of a label-free Optical Imaging Technique—surface plasmon resonance (SPR)—to quantitatively investigate the relative thickness of complex biomolecular structures using a nanoimprinted plasmonic crystal and laboratory microscope. Polyelectrolyte films of different thicknesses deposited by layer-by-layer assembly served as the model system to calibrate the reflection contrast response originating from SPRs. The calibrated SPR system allows quantitative analysis of the thicknesses of the interface formed between the cell culture substrate and cellular membrane regions of fixed Aplysia californica pedal ganglion neurons. Bandpass filters were used to isolate spectral regions of reflected light with distinctive image...

  • quantitative reflection Imaging of fixed aplysia californica pedal ganglion neurons on nanostructured plasmonic crystals
    Journal of Physical Chemistry B, 2013
    Co-Authors: Somi Kang, Lucas B Thompson, Stanislav S Rubakhin, Jonathan V Sweedler, John A Rogers, Ralph G Nuzzo
    Abstract:

    Studies of the interactions between cells and surrounding environment including cell culture surfaces and their responses to distinct chemical and physical cues are essential to understanding the regulation of cell growth, migration, and differentiation. In this work, we demonstrate the capability of a label-free Optical Imaging Technique-surface plasmon resonance (SPR)-to quantitatively investigate the relative thickness of complex biomolecular structures using a nanoimprinted plasmonic crystal and laboratory microscope. Polyelectrolyte films of different thicknesses deposited by layer-by-layer assembly served as the model system to calibrate the reflection contrast response originating from SPRs. The calibrated SPR system allows quantitative analysis of the thicknesses of the interface formed between the cell culture substrate and cellular membrane regions of fixed Aplysia californica pedal ganglion neurons. Bandpass filters were used to isolate spectral regions of reflected light with distinctive image contrast changes. Combining of the data from images acquired using different bandpass filters leads to increase image contrast and sensitivity to topological differences in interface thicknesses. This SPR-based Imaging Technique is restricted in measurable thickness range (∼100-200 nm) due to the limited plasmonic sensing volume, but we complement this Technique with an interferometric analysis method. Described here simple reflection Imaging Techniques show promise as quantitative methods for analyzing surface thicknesses at nanometer scale over large areas in real-time and in physicochemical diverse environments.