The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
Lihong V Wang - One of the best experts on this subject based on the ideXlab platform.
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handheld Optical Resolution photoacoustic microscopy
Journal of Biomedical Optics, 2016Co-Authors: Li Lin, Lidai Wang, Junhui Shi, Junjie Yao, Pengfei Zhang, Jun Zou, Lihong V WangAbstract:Optical-Resolution photoacoustic microscopy (OR-PAM) offers label-free in vivo imaging with high spatial Resolution by acoustically detecting Optical absorption contrasts via the photoacoustic effect. We developed a compact handheld OR-PAM probe for fast photoacoustic imaging. Different from benchtop microscopes, the handheld probe provides flexibility in imaging various anatomical sites. Resembling a cup in size, the probe uses a two-axis water-immersible microelectromechanical system mirror to scan both the illuminating Optical beam and resultant acoustic beam. The system performance was tested in vivo by imaging the capillary bed in a mouse ear and both the capillary bed and a mole on a human volunteer.
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Multiview Optical Resolution photoacoustic microscopy
Optica, 2014Co-Authors: Liren Zhu, Liang Gao, Lihong V WangAbstract:Optical Resolution photoacoustic microscopy (OR-PAM), while providing high lateral Resolution, has been limited by its relatively poor acoustically determined axial Resolution. Although this limitation has been tackled in recent works by using either broadband acoustic detection or nonlinear photoacoustic effects, a flexible solution with three-dimensional Optical Resolution in reflection mode remains desired. Herein we present a multiview OR-PAM technique. By imaging the sample from multiple view angles and reconstructing the data using a multiview deconvolution method, we have experimentally demonstrated an isotropic Optical Resolution in three dimensions.
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near infrared Optical Resolution photoacoustic microscopy
Optics Letters, 2014Co-Authors: Pengfei Hai, Konstantin Maslov, Junjie Yao, Yong Zhou, Lihong V WangAbstract:Compared with visible light (380–700 nm), near-infrared light (700–1400 nm) undergoes weaker Optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared Optical-Resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using Optical fluence within the American National Standards Institute (ANSI) limit (100 mJ/cm^2). Beyond ∼0.6 mm deep in chicken breast tissue, NIR-OR-PAM has shown finer Resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.
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fully motorized Optical Resolution photoacoustic microscopy
IEEE Transactions on Biomedical Engineering, 2014Co-Authors: Chenghung Yeh, Kirk K. Shung, Konstantin Maslov, Lidai Wang, Ruimin Chen, Qifa Zhou, Brian T Soetikno, Lihong V WangAbstract:Fully motorized Optical-Resolution photoacoustic microscopy integrates five complementary scanning modes and simultaneously provides a high imaging speed (2 kHz B-scan rate) and a large field of view (10×8 mm2) for fast quantitative imaging.
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Near-infrared Optical-Resolution Photoacoustic Microscopy with 1046 nm Illumination
Biomedical Optics 2014, 2014Co-Authors: Pengfei Hai, Konstantin Maslov, Junjie Yao, Yong Zhou, Lihong V WangAbstract:Due to weaker scattering, near-infrared light can penetrate deep in tissue. We demonstrate near-infrared Optical-Resolution photoacoustic microscopy with 1046 nm illumination and validate its deep imaging capability both in phantoms and in vivo.
Konstantin Maslov - One of the best experts on this subject based on the ideXlab platform.
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Optical-Resolution photoacoustic endomicroscopy in vivo
Biomedical optics express, 2015Co-Authors: Joon-mo Yang, Konstantin Maslov, Ruimin Chen, Qifa Zhou, Junjie Yao, Chenghung Yeh, Bin Rao, Amos Danielli, Kirk K. ShungAbstract:Optical-Resolution photoacoustic microscopy (OR-PAM) has become a major experimental tool of photoacoustic tomography, with unique imaging capabilities for various biological applications. However, conventional imaging systems are all table-top embodiments, which preclude their use in internal organs. In this study, by applying the OR-PAM concept to our recently developed endoscopic technique, called photoacoustic endoscopy (PAE), we created an Optical-Resolution photoacoustic endomicroscopy (OR-PAEM) system, which enables internal organ imaging with a much finer Resolution than conventional acoustic-Resolution PAE systems. OR-PAEM has potential preclinical and clinical applications using either endogenous or exogenous contrast agents.
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near infrared Optical Resolution photoacoustic microscopy
Optics Letters, 2014Co-Authors: Pengfei Hai, Konstantin Maslov, Junjie Yao, Yong Zhou, Lihong V WangAbstract:Compared with visible light (380–700 nm), near-infrared light (700–1400 nm) undergoes weaker Optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared Optical-Resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using Optical fluence within the American National Standards Institute (ANSI) limit (100 mJ/cm^2). Beyond ∼0.6 mm deep in chicken breast tissue, NIR-OR-PAM has shown finer Resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.
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fully motorized Optical Resolution photoacoustic microscopy
IEEE Transactions on Biomedical Engineering, 2014Co-Authors: Chenghung Yeh, Kirk K. Shung, Konstantin Maslov, Lidai Wang, Ruimin Chen, Qifa Zhou, Brian T Soetikno, Lihong V WangAbstract:Fully motorized Optical-Resolution photoacoustic microscopy integrates five complementary scanning modes and simultaneously provides a high imaging speed (2 kHz B-scan rate) and a large field of view (10×8 mm2) for fast quantitative imaging.
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Near-infrared Optical-Resolution Photoacoustic Microscopy with 1046 nm Illumination
Biomedical Optics 2014, 2014Co-Authors: Pengfei Hai, Konstantin Maslov, Junjie Yao, Yong Zhou, Lihong V WangAbstract:Due to weaker scattering, near-infrared light can penetrate deep in tissue. We demonstrate near-infrared Optical-Resolution photoacoustic microscopy with 1046 nm illumination and validate its deep imaging capability both in phantoms and in vivo.
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wide field two dimensional multifocal Optical Resolution photoacoustic computed microscopy
Optics Letters, 2013Co-Authors: Jun Xia, Konstantin Maslov, Lidai Wang, Mohammadreza Nasiriavanaki, John A Engelbach, Joel R Garbow, Lihong V WangAbstract:Optical-Resolution photoacoustic microscopy (OR-PAM) is an emerging technique that directly images Optical absorption in tissue at high spatial Resolution. To date, the majority of OR-PAM systems are based on single-focused Optical excitation and ultrasonic detection, limiting the wide-field imaging speed. While 1D multifocal OR-PAM (1D-MFOR-PAM) has been developed, the potential of microlens and transducer arrays has not been fully realized. Here we present the development of 2D multifocal Optical-Resolution photoacoustic-computed microscopy (2D-MFOR-PACM), using a 2D microlens array and a full-ring ultrasonic transducer array. The 10 mm×10 mm microlens array generates 1800 Optical foci within the focal plane of the 512-element transducer array, and raster scanning the microlens array yields Optical-Resolution photoacoustic images. The system has improved the in-plane Resolution of a full-ring transducer array from ≥100 to 29 μm and achieved an imaging time of 36 s over a 10 mm×10 mm field of view. In comparison, the 1D-MFOR-PAM would take more than 4 min to image over the same field of view. The imaging capability of the system was demonstrated on phantoms and animals both ex vivo and in vivo.
Lidai Wang - One of the best experts on this subject based on the ideXlab platform.
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handheld Optical Resolution photoacoustic microscopy
Journal of Biomedical Optics, 2016Co-Authors: Li Lin, Lidai Wang, Junhui Shi, Junjie Yao, Pengfei Zhang, Jun Zou, Lihong V WangAbstract:Optical-Resolution photoacoustic microscopy (OR-PAM) offers label-free in vivo imaging with high spatial Resolution by acoustically detecting Optical absorption contrasts via the photoacoustic effect. We developed a compact handheld OR-PAM probe for fast photoacoustic imaging. Different from benchtop microscopes, the handheld probe provides flexibility in imaging various anatomical sites. Resembling a cup in size, the probe uses a two-axis water-immersible microelectromechanical system mirror to scan both the illuminating Optical beam and resultant acoustic beam. The system performance was tested in vivo by imaging the capillary bed in a mouse ear and both the capillary bed and a mole on a human volunteer.
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fully motorized Optical Resolution photoacoustic microscopy
IEEE Transactions on Biomedical Engineering, 2014Co-Authors: Chenghung Yeh, Kirk K. Shung, Konstantin Maslov, Lidai Wang, Ruimin Chen, Qifa Zhou, Brian T Soetikno, Lihong V WangAbstract:Fully motorized Optical-Resolution photoacoustic microscopy integrates five complementary scanning modes and simultaneously provides a high imaging speed (2 kHz B-scan rate) and a large field of view (10×8 mm2) for fast quantitative imaging.
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wide field two dimensional multifocal Optical Resolution photoacoustic computed microscopy
Optics Letters, 2013Co-Authors: Jun Xia, Konstantin Maslov, Lidai Wang, Mohammadreza Nasiriavanaki, John A Engelbach, Joel R Garbow, Lihong V WangAbstract:Optical-Resolution photoacoustic microscopy (OR-PAM) is an emerging technique that directly images Optical absorption in tissue at high spatial Resolution. To date, the majority of OR-PAM systems are based on single-focused Optical excitation and ultrasonic detection, limiting the wide-field imaging speed. While 1D multifocal OR-PAM (1D-MFOR-PAM) has been developed, the potential of microlens and transducer arrays has not been fully realized. Here we present the development of 2D multifocal Optical-Resolution photoacoustic-computed microscopy (2D-MFOR-PACM), using a 2D microlens array and a full-ring ultrasonic transducer array. The 10 mm×10 mm microlens array generates 1800 Optical foci within the focal plane of the 512-element transducer array, and raster scanning the microlens array yields Optical-Resolution photoacoustic images. The system has improved the in-plane Resolution of a full-ring transducer array from ≥100 to 29 μm and achieved an imaging time of 36 s over a 10 mm×10 mm field of view. In comparison, the 1D-MFOR-PAM would take more than 4 min to image over the same field of view. The imaging capability of the system was demonstrated on phantoms and animals both ex vivo and in vivo.
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fast voice coil scanning Optical Resolution photoacoustic microscopy
Optics Letters, 2011Co-Authors: Lidai Wang, Konstantin Maslov, Junjie Yao, Bin Rao, Lihong V WangAbstract:We developed a photoacoustic imaging system that has real-time imaging capability with Optical Resolution. The imaging system is capable of scanning at 20 Hz over a 9 mm range and up to 40 Hz over a 1 mm scanning range. A focused laser beam provides a lateral Resolution of 3.4 μm as measured in an Optically nonscattering medium. Flows of micrometer-sized carbon particles or whole blood in a silicone tube and individual red blood cells (RBCs) in mouse ear capillaries were also imaged in real time, demonstrating the capability to image highly dynamic processes in vivo at a micrometer-scale Resolution.
Liang Song - One of the best experts on this subject based on the ideXlab platform.
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blind deconvolution Optical Resolution photoacoustic microscopy in vivo
Optics Express, 2013Co-Authors: Jianhua Chen, Riqiang Lin, Huina Wang, Jing Meng, Hairong Zheng, Liang SongAbstract:Optical-Resolution photoacoustic microscopy (OR-PAM) is becoming a vital tool for studying the microcirculation system in vivo. By increasing the numerical aperture of Optical focusing, the lateral Resolution of OR-PAM can be improved; however, the depth of focus and thus the imaging range will be sacrificed correspondingly. In this work, we report our development of blind-deconvolution Optical-Resolution photoacoustic microscopy (BD-PAM) that can provide a lateral Resolution ~2-fold finer than that of conventional OR-PAM (3.04 vs. 5.78μm), without physically increasing the system's numerical aperture. The improvement achieved with BD-PAM is demonstrated by imaging graphene nanoparticles and the microvasculature of mice ears in vivo. Our results suggest that BD-PAM may become a valuable tool for many biomedical applications that require both fine spatial Resolution and extended depth of focus.
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In vivo Optical-Resolution photoacoustic computed tomography with compressed sensing.
Optics letters, 2012Co-Authors: Jing Meng, Lihong V Wang, Dong Liang, Liang SongAbstract:Optical-Resolution photoacoustic microscopy is becoming a powerful research tool for studying microcirculation in vivo. Moreover, ultrasonic-array-based Optical-Resolution photoacoustic computed tomography (OR-PACT), providing comparable Resolution at an improved speed, has opened up new opportunities for studying microvascular dynamics. In this Letter, we have developed a compressed sensing with partially known support (CS-PKS) photoacoustic reconstruction strategy for OR-PACT. Compared with conventional backprojection reconstruction, the CS-PKS strategy was shown to produce high-quality in vivo OR-PACT images with threefold less measurement data, which can be leveraged to improve the data acquisition speed and costs of OR-PACT systems.
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multifocal Optical Resolution photoacoustic microscopy in vivo
Optics Letters, 2011Co-Authors: Liang Song, Konstantin Maslov, Lihong V WangAbstract:Although ultrasound arrays have been exploited in photoacoustic imaging to improve imaging speed, ultrasound-array-based Optical-Resolution photoacoustic microscopy (OR-PAM) has never been achieved previously to our knowledge. Here we present our development of multifocal OR-PAM using a microlens array for Optical illumination and an ultrasound array for photoacoustic detection. Our system is capable of imaging hemoglobin concentration and oxygenation in individual microvessels in vivo at high speed. Compared with a single focus, multiple foci reduce the scanning load and increase the imaging speed significantly. The current multifocal system can acquire 1000×500×200 voxels at ∼10 μm lateral Resolution within 4 min.
Junjie Yao - One of the best experts on this subject based on the ideXlab platform.
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handheld Optical Resolution photoacoustic microscopy
Journal of Biomedical Optics, 2016Co-Authors: Li Lin, Lidai Wang, Junhui Shi, Junjie Yao, Pengfei Zhang, Jun Zou, Lihong V WangAbstract:Optical-Resolution photoacoustic microscopy (OR-PAM) offers label-free in vivo imaging with high spatial Resolution by acoustically detecting Optical absorption contrasts via the photoacoustic effect. We developed a compact handheld OR-PAM probe for fast photoacoustic imaging. Different from benchtop microscopes, the handheld probe provides flexibility in imaging various anatomical sites. Resembling a cup in size, the probe uses a two-axis water-immersible microelectromechanical system mirror to scan both the illuminating Optical beam and resultant acoustic beam. The system performance was tested in vivo by imaging the capillary bed in a mouse ear and both the capillary bed and a mole on a human volunteer.
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Optical-Resolution photoacoustic endomicroscopy in vivo
Biomedical optics express, 2015Co-Authors: Joon-mo Yang, Konstantin Maslov, Ruimin Chen, Qifa Zhou, Junjie Yao, Chenghung Yeh, Bin Rao, Amos Danielli, Kirk K. ShungAbstract:Optical-Resolution photoacoustic microscopy (OR-PAM) has become a major experimental tool of photoacoustic tomography, with unique imaging capabilities for various biological applications. However, conventional imaging systems are all table-top embodiments, which preclude their use in internal organs. In this study, by applying the OR-PAM concept to our recently developed endoscopic technique, called photoacoustic endoscopy (PAE), we created an Optical-Resolution photoacoustic endomicroscopy (OR-PAEM) system, which enables internal organ imaging with a much finer Resolution than conventional acoustic-Resolution PAE systems. OR-PAEM has potential preclinical and clinical applications using either endogenous or exogenous contrast agents.
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near infrared Optical Resolution photoacoustic microscopy
Optics Letters, 2014Co-Authors: Pengfei Hai, Konstantin Maslov, Junjie Yao, Yong Zhou, Lihong V WangAbstract:Compared with visible light (380–700 nm), near-infrared light (700–1400 nm) undergoes weaker Optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared Optical-Resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using Optical fluence within the American National Standards Institute (ANSI) limit (100 mJ/cm^2). Beyond ∼0.6 mm deep in chicken breast tissue, NIR-OR-PAM has shown finer Resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.
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Near-infrared Optical-Resolution Photoacoustic Microscopy with 1046 nm Illumination
Biomedical Optics 2014, 2014Co-Authors: Pengfei Hai, Konstantin Maslov, Junjie Yao, Yong Zhou, Lihong V WangAbstract:Due to weaker scattering, near-infrared light can penetrate deep in tissue. We demonstrate near-infrared Optical-Resolution photoacoustic microscopy with 1046 nm illumination and validate its deep imaging capability both in phantoms and in vivo.
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fast voice coil scanning Optical Resolution photoacoustic microscopy
Optics Letters, 2011Co-Authors: Lidai Wang, Konstantin Maslov, Junjie Yao, Bin Rao, Lihong V WangAbstract:We developed a photoacoustic imaging system that has real-time imaging capability with Optical Resolution. The imaging system is capable of scanning at 20 Hz over a 9 mm range and up to 40 Hz over a 1 mm scanning range. A focused laser beam provides a lateral Resolution of 3.4 μm as measured in an Optically nonscattering medium. Flows of micrometer-sized carbon particles or whole blood in a silicone tube and individual red blood cells (RBCs) in mouse ear capillaries were also imaged in real time, demonstrating the capability to image highly dynamic processes in vivo at a micrometer-scale Resolution.
