The Experts below are selected from a list of 33576 Experts worldwide ranked by ideXlab platform
Yann R Chemla - One of the best experts on this subject based on the ideXlab platform.
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ultrahigh resolution Optical Trap with single fluorophore sensitivity
Nature Methods, 2011Co-Authors: Matthew J Comstock, Taekjip Ha, Yann R ChemlaAbstract:We present a single-molecule instrument that combines a time-shared ultrahigh-resolution dual Optical Trap interlaced with a confocal fluorescence microscope. in a demonstration experiment, we observed individual single fluorophore–labeled dn A oligonucleotides to bind and unbind complementary dn A suspended between two Trapped beads. s imultaneous with the single-fluorophore detection, we clearly observed coincident angstrom-scale changes in tether extension. Fluorescence readout allowed us to determine the duplex melting rate as a function of force. t he new instrument will enable the simultaneous measurement of angstrom-scale mechanical motion of individual dn A-binding proteins (for example, single-base-pair stepping of dn A translocases) along with the detection of properties of fluorescently labeled protein (for example, internal configuration).
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Ultrahigh-resolution Optical Trap with single-fluorophore sensitivity
Nature Methods, 2011Co-Authors: Matthew J Comstock, Taekjip Ha, Yann R ChemlaAbstract:The combination of an ultrahigh-resolution dual Optical Trap with a confocal microscope allowed single-fluorophore detection of labeled oligonucleotide binding and simultaneous measurement of angstrom-scale changes in DNA tether extension. We present a single-molecule instrument that combines a time-shared ultrahigh-resolution dual Optical Trap interlaced with a confocal fluorescence microscope. In a demonstration experiment, we observed individual single fluorophore–labeled DNA oligonucleotides to bind and unbind complementary DNA suspended between two Trapped beads. Simultaneous with the single-fluorophore detection, we clearly observed coincident angstrom-scale changes in tether extension. Fluorescence readout allowed us to determine the duplex melting rate as a function of force. The new instrument will enable the simultaneous measurement of angstrom-scale mechanical motion of individual DNA-binding proteins (for example, single-base-pair stepping of DNA translocases) along with the detection of properties of fluorescently labeled protein (for example, internal configuration).
F Ferlaino - One of the best experts on this subject based on the ideXlab platform.
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narrow line magneto Optical Trap for erbium
Physical Review A, 2012Co-Authors: A Frisch, Kiyotaka Aikawa, Manfred J Mark, A Rietzler, J Schindler, Erik Zupanic, R Grimm, F FerlainoAbstract:We report on the experimental realization of a robust and efficient magneto-Optical Trap for erbium atoms, based on a narrow cooling transition at 583 nm. We observe up to $N=2\ifmmode\times\else\texttimes\fi{}{10}^{8}$ atoms at a temperature of about $T=15$ $\ensuremath{\mu}$K. This simple scheme provides better starting conditions for direct loading of dipole Traps as compared to approaches based on the strong cooling transition alone, or on a combination of a strong and a narrow kHz transition. Our results on Er point to a general, simple, and efficient approach to laser cool samples of other lanthanide atoms (Ho, Dy, and Tm) for the production of quantum-degenerate samples.
Taekjip Ha - One of the best experts on this subject based on the ideXlab platform.
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ultrahigh resolution Optical Trap with single fluorophore sensitivity
Nature Methods, 2011Co-Authors: Matthew J Comstock, Taekjip Ha, Yann R ChemlaAbstract:We present a single-molecule instrument that combines a time-shared ultrahigh-resolution dual Optical Trap interlaced with a confocal fluorescence microscope. in a demonstration experiment, we observed individual single fluorophore–labeled dn A oligonucleotides to bind and unbind complementary dn A suspended between two Trapped beads. s imultaneous with the single-fluorophore detection, we clearly observed coincident angstrom-scale changes in tether extension. Fluorescence readout allowed us to determine the duplex melting rate as a function of force. t he new instrument will enable the simultaneous measurement of angstrom-scale mechanical motion of individual dn A-binding proteins (for example, single-base-pair stepping of dn A translocases) along with the detection of properties of fluorescently labeled protein (for example, internal configuration).
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Ultrahigh-resolution Optical Trap with single-fluorophore sensitivity
Nature Methods, 2011Co-Authors: Matthew J Comstock, Taekjip Ha, Yann R ChemlaAbstract:The combination of an ultrahigh-resolution dual Optical Trap with a confocal microscope allowed single-fluorophore detection of labeled oligonucleotide binding and simultaneous measurement of angstrom-scale changes in DNA tether extension. We present a single-molecule instrument that combines a time-shared ultrahigh-resolution dual Optical Trap interlaced with a confocal fluorescence microscope. In a demonstration experiment, we observed individual single fluorophore–labeled DNA oligonucleotides to bind and unbind complementary DNA suspended between two Trapped beads. Simultaneous with the single-fluorophore detection, we clearly observed coincident angstrom-scale changes in tether extension. Fluorescence readout allowed us to determine the duplex melting rate as a function of force. The new instrument will enable the simultaneous measurement of angstrom-scale mechanical motion of individual DNA-binding proteins (for example, single-base-pair stepping of DNA translocases) along with the detection of properties of fluorescently labeled protein (for example, internal configuration).
Matthew J Comstock - One of the best experts on this subject based on the ideXlab platform.
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ultrahigh resolution Optical Trap with single fluorophore sensitivity
Nature Methods, 2011Co-Authors: Matthew J Comstock, Taekjip Ha, Yann R ChemlaAbstract:We present a single-molecule instrument that combines a time-shared ultrahigh-resolution dual Optical Trap interlaced with a confocal fluorescence microscope. in a demonstration experiment, we observed individual single fluorophore–labeled dn A oligonucleotides to bind and unbind complementary dn A suspended between two Trapped beads. s imultaneous with the single-fluorophore detection, we clearly observed coincident angstrom-scale changes in tether extension. Fluorescence readout allowed us to determine the duplex melting rate as a function of force. t he new instrument will enable the simultaneous measurement of angstrom-scale mechanical motion of individual dn A-binding proteins (for example, single-base-pair stepping of dn A translocases) along with the detection of properties of fluorescently labeled protein (for example, internal configuration).
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Ultrahigh-resolution Optical Trap with single-fluorophore sensitivity
Nature Methods, 2011Co-Authors: Matthew J Comstock, Taekjip Ha, Yann R ChemlaAbstract:The combination of an ultrahigh-resolution dual Optical Trap with a confocal microscope allowed single-fluorophore detection of labeled oligonucleotide binding and simultaneous measurement of angstrom-scale changes in DNA tether extension. We present a single-molecule instrument that combines a time-shared ultrahigh-resolution dual Optical Trap interlaced with a confocal fluorescence microscope. In a demonstration experiment, we observed individual single fluorophore–labeled DNA oligonucleotides to bind and unbind complementary DNA suspended between two Trapped beads. Simultaneous with the single-fluorophore detection, we clearly observed coincident angstrom-scale changes in tether extension. Fluorescence readout allowed us to determine the duplex melting rate as a function of force. The new instrument will enable the simultaneous measurement of angstrom-scale mechanical motion of individual DNA-binding proteins (for example, single-base-pair stepping of DNA translocases) along with the detection of properties of fluorescently labeled protein (for example, internal configuration).
Matthias Weidemüller - One of the best experts on this subject based on the ideXlab platform.
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Two-dimensional magneto-Optical Trap as a source for cold strontium atoms
Physical Review A, 2017Co-Authors: Ingo Nosske, Luc Couturier, Canzhu Tan, Chang Qiao, Jan Blume, Y. H. Jiang, Peng Chen, Matthias WeidemüllerAbstract:We report on the realization of a transversely loaded two-dimensional magneto-Optical Trap serving as a source for cold strontium atoms. We analyze the dependence of the source's properties on various parameters, in particular the intensity of a pushing beam accelerating the atoms out of the source. An atomic flux exceeding $10^9\,\mathrm{atoms/s}$ at a rather moderate oven temperature of $500\,^\circ\mathrm{C}$ is achieved. The longitudinal velocity of the atomic beam can be tuned over several tens of m/s by adjusting the power of the pushing laser beam. The beam divergence is around $60$ mrad, determined by the transverse velocity distribution of the cold atoms. The slow atom source is used to load a three-dimensional magneto-Optical Trap realizing loading rates up to $10^9\,\mathrm{atoms/s}$ without indication of saturation of the loading rate for increasing oven temperature. The compact setup avoids undesired effects found in alternative sources like, e.g., Zeeman slowers, such as vacuum contamination and black-body radiation due to the hot strontium oven
