The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Nomdo M Jansonius - One of the best experts on this subject based on the ideXlab platform.
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glaucoma screening during regular Optician visits the feasibility and specificity of screening in real life
Acta Ophthalmologica, 2012Co-Authors: Margriet M De Vries, Remco Stoutenbeek, Rogier P H M Muskens, Nomdo M JansoniusAbstract:Purpose: To determine the feasibility and specificity of glaucoma screening during regular Optician visits. Methods: In four Optician shops, glaucoma screening was offered to 400 consecutive visitors aged 45 years or above. If the visitor agreed to participate, an intraocular pressure measurement and - in those with a pressure below 25 mmHg - a frequency-doubling perimeter (FDT) C20-1 visual field screening test were performed. Those with an elevated pressure or at least one reproducibly abnormal test location on FDT were referred to our hospital. Results: Three-hundred and fifty-two of 400 consecutive visitors (88%) were screened. Fifteen of the unscreened visitors were not screened because they were already regularly checked by an ophthalmologist related to glaucoma. Forty-two of 352 screened participants (12%) were referred. Of these 42 referrals, seven were diagnosed with glaucoma, 10 were diagnosed with ocular hypertension (OHT), 12 did not have any eye disease, seven had an eye disease other than glaucoma or OHT that was diagnosed previously and six were newly diagnosed with an eye disease other than glaucoma or OHT. The specificity of the screening protocol was 91% (95% confidence interval 88-94%). Conclusions: Glaucoma screening at the Optician shop was feasible, but the specificity of the screening protocol was rather low. With more stringent cut-off points (30 mmHg; at least two reproducibly abnormal test locations), the specificity could be improved to 96% (94-98%), apparently without a significant loss of sensitivity. This suggests that screening during regular Optician visits might be a viable approach.
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glaucoma screening during regular Optician visits can the population at risk of developing glaucoma be reached
British Journal of Ophthalmology, 2006Co-Authors: Remco Stoutenbeek, Nomdo M JansoniusAbstract:Aim: To determine the percentage of the population at risk of developing glaucoma, which can potentially be reached by conducting glaucoma screening during regular Optician visits. Methods: 1200 inhabitants aged >40 years were randomly selected from Dutch community population databases. A questionnaire was mailed to these inhabitants with questions on their latest Optician visit and risk factors for glaucoma. A second questionnaire was sent to their Opticians, who were asked about their willingness to conduct an additional glaucoma screening programme in the future. Results: The questionnaire was returned by 959 of 1200 inhabitants and 37 of 50 Opticians. The percentage of inhabitants who visited an Optician during a 5-year period was 83% (95% confidence interval (CI) 80% to 85%). This percentage was adjusted for the presence of risk factors for glaucoma to obtain the percentage of the population at risk of developing glaucoma. The percentage of Opticians willing to cooperate in a glaucoma screening programme extended beyond a non-contact tonometry measurement alone was 91% (95% CI 77% to 98%). Conclusion: By conducting glaucoma screening during regular Optician visits, a large section of the population at risk of developing glaucoma can be reached.
Katherine Peterson - One of the best experts on this subject based on the ideXlab platform.
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expressed sequence tag analysis of human rpe choroid for the neibank project over 6000 non redundant transcripts novel genes and splice variants
Molecular Vision, 2002Co-Authors: Graeme Wistow, Steven L Bernstein, M K Wyatt, Robert N Fariss, A Behal, J W Touchman, G Bouffard, D Smith, Katherine PetersonAbstract:PURPOSE The retinal pigment epithelium (RPE) and choroid comprise a functional unit of the eye that is essential to normal retinal health and function. Here we describe expressed sequence tag (EST) analysis of human RPE/choroid as part of a project for ocular bioinformatics. METHODS A cDNA library (cs) was made from human RPE/choroid and sequenced. Data were analyzed and assembled using the program GRIST (GRouping and Identification of Sequence Tags). Complete sequencing, Northern and Western blots, RH mapping, peptide antibody synthesis and immunofluorescence (IF) have been used to examine expression patterns and genome location for selected transcripts and proteins. RESULTS Ten thousand individual sequence reads yield over 6300 unique gene clusters of which almost half have no matches with named genes. One of the most abundant transcripts is from a gene (named "alpha") that maps to the BBS1 region of chromosome 11. A number of tissue preferred transcripts are common to both RPE/choroid and iris. These include oculoglycan/opticin, for which an alternative splice form is detected in RPE/choroid, and "oculospanin" (Ocsp), a novel tetraspanin that maps to chromosome 17q. Antiserum to Ocsp detects expression in RPE, iris, ciliary body, and retinal ganglion cells by IF. A newly identified gene for a zinc-finger protein (TIRC) maps to 19q13.4. Variant transcripts of several genes were also detected. Most notably, the predominant form of Bestrophin represented in cs contains a longer open reading frame as a result of splice junction skipping. CONCLUSIONS The unamplified cs library gives a view of the transcriptional repertoire of the adult RPE/choroid. A large number of potentially novel genes and splice forms and candidates for genetic diseases are revealed. Clones from this collection are being included in a large, nonredundant set for cDNA microarray construction.
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expressed sequence tag analysis of adult human iris for the neibank project steroid response factors and similarities with retinal pigment epithelium
Molecular Vision, 2002Co-Authors: Graeme Wistow, Steven L Bernstein, M K Wyatt, A Behal, J W Touchman, G Bouffard, D Smith, Katherine PetersonAbstract:PURPOSE: The iris is a specialized tissue with important roles in the development and function of the eye. It is involved in diseases, including glaucoma and ocular melanoma, and its pigmented cells share an origin with the retinal pigment epithelium (RPE). Expressed sequence tag (EST) analysis of human iris has been performed to explore the repertoire of genes expressed in this tissue. METHODS: An unamplified, un-normalized cDNA library (designated bx) was constructed from pooled (4-80 years old) human iris tissue. Over 2000 clones were picked and sequenced. Sequences were analyzed and clustered using GRIST (GRouping and Identification of Sequence Tags). The library was then normalized (and designated fg) and a further 2200 clones were sequenced for deeper examination of rarer sequence. Some sequences of interest were investigated further by standard methods. RESULTS: From bx and fg libraries respectively, 1263 and 1604 clusters of expressed genes have been identified, giving a combined total of almost 2700 potentially unique genes. The most abundant novel transcript in bx is oculoglycan/opticin. Others include glucocorticoid induced leucine zipper protein (GILZ), Ris, a novel member of the Ras family, Iris Ring Finger (IRF), a member of the midline family, melastatin 2 (MLSN2), a member of the transient receptor potential calcium channel family, and iris expressed growth factor (IEGF), a member of the VEGF/PDGF family. Several factors involved in steroid responses are also represented. CONCLUSIONS: The iris libraries are a rich source of novel as well as known genes, including molecular markers for pigmented cells that are also shared with RPE. A number of transcripts code for proteins involved in steroid response, with interesting implications for control of intraocular pressure. These sequence verified clones provide a nonredundant set for micro-array construction.
Anna Mills - One of the best experts on this subject based on the ideXlab platform.
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Bringing the Effects of Occupational Licensing into Focus: Optician Licensing in the United States
Eastern Economic Journal, 2016Co-Authors: Edward J Timmons, Anna MillsAbstract:The labor market institution of occupational licensing continues to grow in scope in the United States and abroad. In this paper, we estimate the effects of occupational licensing on Opticians using data from the US Census and American Community Survey. Our results suggest that Optician licensing is associated with Opticians receiving as much as 16.9 percent more in annual earnings. In an examination of malpractice insurance premiums in all states and participation rates in Optician certification programs in Texas, we find little evidence that Optician licensing has enhanced the quality of services delivered to consumers. By and large, Optician licensing appears to be reducing consumer welfare by raising the earnings of Opticians without enhancing the quality of services delivered to consumers.
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bringing the effects of occupational licensing into focus Optician licensing in the united states
Social Science Research Network, 2015Co-Authors: Edward Timmons, Anna MillsAbstract:The scope and scale of occupational licensing in the United States continues to grow. Our study finds that stricter Optician licensing laws and laws that have been in place for longer periods of time result in higher Optician earnings. On the other hand, there is little evidence that licensing has affected vision insurance or Optician malpractice insurance premiums. Optician licensing increases Optician earnings with no measurable benefit to consumers.
Graeme Wistow - One of the best experts on this subject based on the ideXlab platform.
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expressed sequence tag analysis of human rpe choroid for the neibank project over 6000 non redundant transcripts novel genes and splice variants
Molecular Vision, 2002Co-Authors: Graeme Wistow, Steven L Bernstein, M K Wyatt, Robert N Fariss, A Behal, J W Touchman, G Bouffard, D Smith, Katherine PetersonAbstract:PURPOSE The retinal pigment epithelium (RPE) and choroid comprise a functional unit of the eye that is essential to normal retinal health and function. Here we describe expressed sequence tag (EST) analysis of human RPE/choroid as part of a project for ocular bioinformatics. METHODS A cDNA library (cs) was made from human RPE/choroid and sequenced. Data were analyzed and assembled using the program GRIST (GRouping and Identification of Sequence Tags). Complete sequencing, Northern and Western blots, RH mapping, peptide antibody synthesis and immunofluorescence (IF) have been used to examine expression patterns and genome location for selected transcripts and proteins. RESULTS Ten thousand individual sequence reads yield over 6300 unique gene clusters of which almost half have no matches with named genes. One of the most abundant transcripts is from a gene (named "alpha") that maps to the BBS1 region of chromosome 11. A number of tissue preferred transcripts are common to both RPE/choroid and iris. These include oculoglycan/opticin, for which an alternative splice form is detected in RPE/choroid, and "oculospanin" (Ocsp), a novel tetraspanin that maps to chromosome 17q. Antiserum to Ocsp detects expression in RPE, iris, ciliary body, and retinal ganglion cells by IF. A newly identified gene for a zinc-finger protein (TIRC) maps to 19q13.4. Variant transcripts of several genes were also detected. Most notably, the predominant form of Bestrophin represented in cs contains a longer open reading frame as a result of splice junction skipping. CONCLUSIONS The unamplified cs library gives a view of the transcriptional repertoire of the adult RPE/choroid. A large number of potentially novel genes and splice forms and candidates for genetic diseases are revealed. Clones from this collection are being included in a large, nonredundant set for cDNA microarray construction.
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expressed sequence tag analysis of adult human iris for the neibank project steroid response factors and similarities with retinal pigment epithelium
Molecular Vision, 2002Co-Authors: Graeme Wistow, Steven L Bernstein, M K Wyatt, A Behal, J W Touchman, G Bouffard, D Smith, Katherine PetersonAbstract:PURPOSE: The iris is a specialized tissue with important roles in the development and function of the eye. It is involved in diseases, including glaucoma and ocular melanoma, and its pigmented cells share an origin with the retinal pigment epithelium (RPE). Expressed sequence tag (EST) analysis of human iris has been performed to explore the repertoire of genes expressed in this tissue. METHODS: An unamplified, un-normalized cDNA library (designated bx) was constructed from pooled (4-80 years old) human iris tissue. Over 2000 clones were picked and sequenced. Sequences were analyzed and clustered using GRIST (GRouping and Identification of Sequence Tags). The library was then normalized (and designated fg) and a further 2200 clones were sequenced for deeper examination of rarer sequence. Some sequences of interest were investigated further by standard methods. RESULTS: From bx and fg libraries respectively, 1263 and 1604 clusters of expressed genes have been identified, giving a combined total of almost 2700 potentially unique genes. The most abundant novel transcript in bx is oculoglycan/opticin. Others include glucocorticoid induced leucine zipper protein (GILZ), Ris, a novel member of the Ras family, Iris Ring Finger (IRF), a member of the midline family, melastatin 2 (MLSN2), a member of the transient receptor potential calcium channel family, and iris expressed growth factor (IEGF), a member of the VEGF/PDGF family. Several factors involved in steroid responses are also represented. CONCLUSIONS: The iris libraries are a rich source of novel as well as known genes, including molecular markers for pigmented cells that are also shared with RPE. A number of transcripts code for proteins involved in steroid response, with interesting implications for control of intraocular pressure. These sequence verified clones provide a nonredundant set for micro-array construction.
Remco Stoutenbeek - One of the best experts on this subject based on the ideXlab platform.
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glaucoma screening during regular Optician visits the feasibility and specificity of screening in real life
Acta Ophthalmologica, 2012Co-Authors: Margriet M De Vries, Remco Stoutenbeek, Rogier P H M Muskens, Nomdo M JansoniusAbstract:Purpose: To determine the feasibility and specificity of glaucoma screening during regular Optician visits. Methods: In four Optician shops, glaucoma screening was offered to 400 consecutive visitors aged 45 years or above. If the visitor agreed to participate, an intraocular pressure measurement and - in those with a pressure below 25 mmHg - a frequency-doubling perimeter (FDT) C20-1 visual field screening test were performed. Those with an elevated pressure or at least one reproducibly abnormal test location on FDT were referred to our hospital. Results: Three-hundred and fifty-two of 400 consecutive visitors (88%) were screened. Fifteen of the unscreened visitors were not screened because they were already regularly checked by an ophthalmologist related to glaucoma. Forty-two of 352 screened participants (12%) were referred. Of these 42 referrals, seven were diagnosed with glaucoma, 10 were diagnosed with ocular hypertension (OHT), 12 did not have any eye disease, seven had an eye disease other than glaucoma or OHT that was diagnosed previously and six were newly diagnosed with an eye disease other than glaucoma or OHT. The specificity of the screening protocol was 91% (95% confidence interval 88-94%). Conclusions: Glaucoma screening at the Optician shop was feasible, but the specificity of the screening protocol was rather low. With more stringent cut-off points (30 mmHg; at least two reproducibly abnormal test locations), the specificity could be improved to 96% (94-98%), apparently without a significant loss of sensitivity. This suggests that screening during regular Optician visits might be a viable approach.
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glaucoma screening during regular Optician visits can the population at risk of developing glaucoma be reached
British Journal of Ophthalmology, 2006Co-Authors: Remco Stoutenbeek, Nomdo M JansoniusAbstract:Aim: To determine the percentage of the population at risk of developing glaucoma, which can potentially be reached by conducting glaucoma screening during regular Optician visits. Methods: 1200 inhabitants aged >40 years were randomly selected from Dutch community population databases. A questionnaire was mailed to these inhabitants with questions on their latest Optician visit and risk factors for glaucoma. A second questionnaire was sent to their Opticians, who were asked about their willingness to conduct an additional glaucoma screening programme in the future. Results: The questionnaire was returned by 959 of 1200 inhabitants and 37 of 50 Opticians. The percentage of inhabitants who visited an Optician during a 5-year period was 83% (95% confidence interval (CI) 80% to 85%). This percentage was adjusted for the presence of risk factors for glaucoma to obtain the percentage of the population at risk of developing glaucoma. The percentage of Opticians willing to cooperate in a glaucoma screening programme extended beyond a non-contact tonometry measurement alone was 91% (95% CI 77% to 98%). Conclusion: By conducting glaucoma screening during regular Optician visits, a large section of the population at risk of developing glaucoma can be reached.
