The Experts below are selected from a list of 2013 Experts worldwide ranked by ideXlab platform
Alberto Quaglia - One of the best experts on this subject based on the ideXlab platform.
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do Orcein positive copper binding protein deposits and cytokeratin 7 co localise in periportal hepatocytes in chronic cholestasis
Journal of Clinical Pathology, 2018Co-Authors: C Starling, Prithi S. Bhathal, Alberto QuagliaAbstract:Orcein stain for copper-binding protein and immunohistochemistry for cytokeratin 7 (K7) are commonly used to detect signs of chronic cholestasis in the context of chronic biliary disease, particularly at an early stage when bilirubinostasis is lacking and in the non-icteric phase of the disease.1 2 Copper-binding protein deposits and K7 expression by periportal hepatocytes occur in a patchy and heterogenous fashion. In a recent study, we have observed that more than half of 82 periportal regions in 12 liver biopsies from patients with chronic cholangiopathies did not show any stain for copper, copper-binding protein or K7.3 Only a minority (9%) of these periportal regions were positive for rhodanine, Orcein and K7 stains. This observation was based on comparing three sections at different levels from each biopsy used for the Orcein, rhodanine and K7 stains. Although these sections were cut serially, they were not suitable to investigate whether copper-binding protein deposits and K7 expression were present in the same hepatocytes. We therefore designed a protocol of sequential histochemical (Orcein) and immunohistochemical (K7) staining on a single-tissue …
M. S. Tanner - One of the best experts on this subject based on the ideXlab platform.
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reversal of indian childhood cirrhosis by d penicillamine therapy
Journal of Pediatric Gastroenterology and Nutrition, 1995Co-Authors: A M Pradhan, Sheila Bhave, Anand Pandit, Ashish Bavdekar, V V Joshi, M. S. TannerAbstract:Serial liver biopsy changes have been reviewed in 30 patients with Indian childhood cirrhosis (ICC) who were randomly allocated to receive treatment with penicillamine in a dose of 20 mg/kg/day, 10 of whom also received prednisolone, and five receiving placebo. The latter died within 185 (mean, 149) days of starting treatment. Nine receiving penicillamine died within 540 (mean, 338) days, but the remainder are well 5.1-9.3 years after commencing treatment. Initial biopsies showed severe hepatocellular injury, pericellular fibrosis, inflammatory infiltration, and Orcein-staining granules. Second biopsies taken within 6 months of starting penicillamine usually showed persistence of inflammation and an increase in nodularity with thick and thin active septae. Subsequently the appearances were of an inactive micronodular cirrhosis, with reduction in septal inflammatory infiltrate, hepatocellular injury, and intensity of Orcein staining. This further improved to a stage of incomplete fibrous septae. The last liver biopsies at 6-60 months (in 21 survivors) showed almost normal histology in four, incomplete fibrous septae in five, and inactive micronodular cirrhosis with thin septae in 12. Mean liver copper concentrations decreased from 1,407 (SEM, 121) micrograms/g at presentation to 925 (183), 317 (100), and 127 (35) at 6, 6-18, and > 18 months after starting treatment. By contrast, a second biopsy taken in the 6 months after diagnosis in placebo-treated children showed persistence of ICC with increase in inflammation, fibrosis, and Orcein staining.
C Starling - One of the best experts on this subject based on the ideXlab platform.
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do Orcein positive copper binding protein deposits and cytokeratin 7 co localise in periportal hepatocytes in chronic cholestasis
Journal of Clinical Pathology, 2018Co-Authors: C Starling, Prithi S. Bhathal, Alberto QuagliaAbstract:Orcein stain for copper-binding protein and immunohistochemistry for cytokeratin 7 (K7) are commonly used to detect signs of chronic cholestasis in the context of chronic biliary disease, particularly at an early stage when bilirubinostasis is lacking and in the non-icteric phase of the disease.1 2 Copper-binding protein deposits and K7 expression by periportal hepatocytes occur in a patchy and heterogenous fashion. In a recent study, we have observed that more than half of 82 periportal regions in 12 liver biopsies from patients with chronic cholangiopathies did not show any stain for copper, copper-binding protein or K7.3 Only a minority (9%) of these periportal regions were positive for rhodanine, Orcein and K7 stains. This observation was based on comparing three sections at different levels from each biopsy used for the Orcein, rhodanine and K7 stains. Although these sections were cut serially, they were not suitable to investigate whether copper-binding protein deposits and K7 expression were present in the same hepatocytes. We therefore designed a protocol of sequential histochemical (Orcein) and immunohistochemical (K7) staining on a single-tissue …
A M Pradhan - One of the best experts on this subject based on the ideXlab platform.
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reversal of indian childhood cirrhosis by d penicillamine therapy
Journal of Pediatric Gastroenterology and Nutrition, 1995Co-Authors: A M Pradhan, Sheila Bhave, Anand Pandit, Ashish Bavdekar, V V Joshi, M. S. TannerAbstract:Serial liver biopsy changes have been reviewed in 30 patients with Indian childhood cirrhosis (ICC) who were randomly allocated to receive treatment with penicillamine in a dose of 20 mg/kg/day, 10 of whom also received prednisolone, and five receiving placebo. The latter died within 185 (mean, 149) days of starting treatment. Nine receiving penicillamine died within 540 (mean, 338) days, but the remainder are well 5.1-9.3 years after commencing treatment. Initial biopsies showed severe hepatocellular injury, pericellular fibrosis, inflammatory infiltration, and Orcein-staining granules. Second biopsies taken within 6 months of starting penicillamine usually showed persistence of inflammation and an increase in nodularity with thick and thin active septae. Subsequently the appearances were of an inactive micronodular cirrhosis, with reduction in septal inflammatory infiltrate, hepatocellular injury, and intensity of Orcein staining. This further improved to a stage of incomplete fibrous septae. The last liver biopsies at 6-60 months (in 21 survivors) showed almost normal histology in four, incomplete fibrous septae in five, and inactive micronodular cirrhosis with thin septae in 12. Mean liver copper concentrations decreased from 1,407 (SEM, 121) micrograms/g at presentation to 925 (183), 317 (100), and 127 (35) at 6, 6-18, and > 18 months after starting treatment. By contrast, a second biopsy taken in the 6 months after diagnosis in placebo-treated children showed persistence of ICC with increase in inflammation, fibrosis, and Orcein staining.
H B Turgut - One of the best experts on this subject based on the ideXlab platform.
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fetus kesitsel anatomisinde mr ve Orcein picroindigocarmine boyama yonteminin karsilastirilmasi
Pamukkale Tıp Dergisi, 2014Co-Authors: Tuncay Peker, Nadir Gulekon, Nil Tokgoz, Suna Omeroglu, A Anil, H B TurgutAbstract:AMAC: Literaturde fetus kesitsel anatomisi ile ilgili calismalar kisitlidir. Bu calismada fetus kesitsel anatomik yapilarini incelenmek amaciyla Orcein-picroindigocarmine boyama yontemi ile fetus manyetik rezonans goruntuleme kesitleri karsilastirilmistir. YONTEMLER: 18-20 haftalik 6 insan fetusunun bas-boyun ve ekstremiteleri once manyetik rezonans ile goruntulendikten sonra kesitler alinarak Orcein-picroindigocarmine ile boyandi. BULGULAR: Bas kesitlerinde Orcein-picroindigocarmine boyama ile saptanabilen kucuk damarlar MR goruntulerinde izlenememistir. Ayrica histolojik kesitlerde 20 haftalik bir fetuste oksipital lobda girus olusumu saptanirken, ayni fetusun MR goruntulerinde boyle bir bulguya rastlanmamistir. Boyun bolgesinde histolojik kesitler ile MR goruntuleri arasinda ince detaylar disinda belirgin farklilik saptanmamisken, ozellikle ekstremitelerde Orcein-picroindigocarmine boyama yumusak doku planlarinin ayirt edilmesinde, kas ve damar-sinir paketinin gosterilmesinde manyetik rezonans goruntulemeye ustun bulunmustur. SONUC: Bu konuda daha detayli calismalarin yapilmasi gerektigi inancindayiz.
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fetus kesitsel anatomisinde mr ve Orcein picroindigocarmine boyama yonteminin karsilastirilmasi comparison of mr imaging and Orcein picroindigocarmine staining method in fetal sectional anatomy
2014Co-Authors: Tuncay Peker, Nadir Gulekon, Nil Tokgoz, Suna Omeroglu, H B TurgutAbstract:Purpose: There are limited fetal MR anatomy studies in the literature. The aim of this study was to compare the Orcein-picroindigocarmine staining method with fetal MR imaging in evaluating sectional fetal anatomic structures. Materials and methods: After MR images of 6 human 18-20 week old fetal sections of the head, neck and extremities were taken, the same sections were stained with Orcein-picroindigocarmine stain. Results: The Orcein-picroindigocarmine staining method had some advantages over MR imaging in regards to neurovascular structures and gyrus formation. Gyrus formation in the occipital lobe of a 20 week old fetus was detected in the histological specimens whereas it was not detected in the MR images of the same fetus. In the neck region, no evident difference, except for slight details, were identified between Orcein-picroindigocarmine staining and MR imaging. Particularly in the extremities, Orcein-picroindigocarmine staining was found to be superior to MR imaging in differentiating between soft tissue planes, muscle, and neuro-vascular bundles. Conclusion: The results from this study warrant further investigation.
