The Experts below are selected from a list of 1296 Experts worldwide ranked by ideXlab platform
Kazuhide Yamasato - One of the best experts on this subject based on the ideXlab platform.
-
phylogenetic analysis of the family Rhizobiaceae and related bacteria by sequencing of 16s rrna gene using pcr and dna sequencer
Fems Microbiology Letters, 1993Co-Authors: Miyoko Yanagi, Kazuhide YamasatoAbstract:The 16S rRNA gene sequences of 19 strains covering 97% of the molecules were determined for the members of the family Rhizobiaceae and related bacteria by PCR and DNA sequencer. The three biovars of Agrobacterium were located separately, whereas Agrobacterium rubi clustered with A. tumefaciens. Phylogenetic locations for the species of the genera Rhizobium, Sinorhizobium, Agrobacterium, Phylobacterium, Mycoplana (M. dimorpha), Ochrobactrum, Brucella and Rochalimaea (a rickettsia) were intermingled with each other with the similarity values higher than 92%. The family Rhizobiaceae should be redefined including the above-mentioned genera despite the ability for plant association and nitrogen fixation. Bradyrhizobium japonicum and Mycoplana bullata were far remote from the other species and should be excluded from this family.
Kristina Lindström - One of the best experts on this subject based on the ideXlab platform.
-
Revised phylogeny of [i]Rhizobiaceae[/i]: Proposal of the delineation of [i]Pararhizobium gen. nov.[/i], and 13 new species combinations
Systematic and Applied Microbiology, 2015Co-Authors: Seyed Abdollah Mousavi, Xavier Nesme, Philippe De Lajudie, Kristina LindströmAbstract:The family Rhizobiaceae accommodates the seven genera Rhizobium, Neorhizobium, Allorhizobium, Agrobacterium, Ensifer (syn. Sinorhizobium), Shinella and Ciceribacter. However, several so-called Rhizobium species do not exhibit robust phylogenetic positions. Rhizobium is extremely heterogeneous and is in need of major revision. Therefore, a phylogenetic examination of the family Rhizobiaceae by multilocus sequence analysis (MLSA) of four housekeeping genes among 100 strains of the family was undertaken. Based on the results we propose the delineation of the new genus Pararhizobium in the Rhizobiaceae family, and 13 new species combinations: Agrobacterium nepotum comb. nov., Agrobacterium pusense comb. nov., Agrobacterium skierniewicense comb. nov., Allorhizobium yids comb. nov., Allorhizobium taibaishanense comb. nov., Allorhizobium paknamense comb. nov., Allorhizobium oryzae comb. nov., Allorhizobium pseudoryzae comb. nov., Allorhizobium borbori comb. nov., Pararhizobium giardinii comb. nov., Pararhizobium capsulatum comb. nov., Pararhizobium herbae comb. nov., and Pararhizobium sphaerophysae comb. nov.
-
Phylogeny of the Rhizobium-Allorhizobium-Agrobacterium clade supports the delineation of Neorhizobium gen. nov.
Systematic and Applied Microbiology, 2014Co-Authors: Seyed Abdollah Mousavi, Céline Lavire, Xavier Nesme, Niklas Wahlberg, Ludovic Vial, Lars Paulin, Philippe De Lajudie, Kristina LindströmAbstract:The genera Agrobacterium, Allorhizobium, and Rhizobium belong to the family Rhizobiaceae. However, the placement of a phytopathogenic group of bacteria, the genus Agrobacterium, among the nitrogen-fixing bacteria and the unclear position of Rhizobium galegae have caused controversy in previous taxonomic studies. To resolve uncertainties in the taxonomy and nomenclature within this family, the phylogenetic relationships of generic members of Rhizobiaceae were studied, but with particular emphasis on the taxa included in Agrobacterium and the "R. galegae complex" (R. galegae and related taxa), using multilocus sequence analysis (MLSA) of six protein-coding housekeeping genes among 114 rhizobial and agrobacterial taxa. The results showed that R. galegae, R. vignae, R. huautlense, and R. alkalisoli formed a separate clade that clearly represented a new genus, for which the name Neorhizobium is proposed. Agrobacterium was shown to represent a separate cluster of mainly pathogenic taxa of the family Rhizobiaceae. A. vitis grouped with Allorhizobium, distinct from Agrobacterium, and should be reclassified as Allorhizobium vitis, whereas Rhizobium rhizogenes was considered to be the proper name for former Agrobacterium rhizogenes. This phylogenetic study further indicated that the taxonomic status of several taxa could be resolved by the creation of more novel genera.
Vitaly Citovsky - One of the best experts on this subject based on the ideXlab platform.
-
from host recognition to t dna integration the function of bacterial and plant genes in the agrobacterium plant cell interaction
Molecular Plant Pathology, 2000Co-Authors: Tzvi Tzfira, Vitaly CitovskyAbstract:Agrobacterium tumefaciens and its related species, A. rhizogenes and A. vitis, are the only known bacterial pathogens which ‘genetically invade’ host plants and stably integrate part of their genetic material into the host cell genome. Thus, A. tumefaciens has evolved as a major tool for plant genetic engineering. Furthermore, this unique process of interkingdom DNA transfer has been utilized as a model system for studies of its underlying biological events, such as intercellular signalling, cell-to-cell DNA transport, protein and DNA nuclear import and integration. To date, numerous bacterial proteins and several plant proteins have been implicated in the A. tumefaciens–plant cell interaction. Here, we discuss the molecular interactions among these bacterial and plant factors and their role in the A. tumefaciens–plant cell DNA transfer. Taxonomic relationship: Bacteria; Proteobacteria; alpha subdivision; Rhizobiaceae group; Rhizobiaceae family; Agrobacterium genus. Microbiological properties: Gram-negative, nonsporing, motile, rod-shaped, soil-borne. Related species:A. rhizogenes (causes root formation in infected plants), A. vitis (causes gall formation on grapevines). Disease symptoms: Formation of neoplastic swellings (galls) on plant roots, crowns, trunks and canes. Galls interfere with water and nutrient flow in the plants, and seriously infected plants suffer from weak, stunted growth and low productivity. Host range: One of the widest host ranges known among plant pathogens; can potentially attack all dicotyledonous plant species. Also, under controlled conditions (usually in tissue culture), can infect, albeit with lower efficiency, several monocotyledonous species. Agronomic importance: The disease currently affects plants belonging to the rose family, e.g. apple, pear, peach, cherry, almond, roses, as well as poplar trees (aspen). Useful web site:http://www.bio.purdue.edu/courses/gelvinweb/gelvin.html
Encarna Velazquez - One of the best experts on this subject based on the ideXlab platform.
-
The Rhizobiaceae Bacteria Transferring Genes to Higher Plants
Horizontal Gene Transfer, 2019Co-Authors: Martha Helena Ramírez-bahena, Alvaro Peix, Encarna VelazquezAbstract:The family Rhizobiaceae includes several bacterial genera able to induce root or stem nodules, which can be beneficial for the plant, or hypertrophies, such as tumours, which cause plant damage. The members from genus Agrobacterium are well known by their ability to transfer genes to different plants originating tumours, and this feature has been biotechnologically exploited to produce transgenic plants. Nevertheless, the taxonomy and phylogeny of this genus has been confusing in the last decades after its reclassification into the genus Rhizobium. The presence of the telomerase-coding gene telA is a unique characteristic of the Agrobacterium clade, and it has been recently recovered as a separate genus. However, some tumour-inducing strains remain classified within genus Rhizobium, and some other species have been reclassified into genus Allorhizobium. The phylogenies of the virulence genes harboured by pTi plasmids inside or outside T-DNA are different as well as the symptoms induced in plants. In this chapter we revise the evolution of the taxonomy of tumorigenic species from family Rhizobiaceae over time, their interactions with different plants, the implications of horizontal gene transfer (HGT) in plant evolution and their use to obtain transgenic plants.
-
maldi tof mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae
PLOS ONE, 2011Co-Authors: Laura Ferreira, Fernando Sanchezjuanes, Paula Garciafraile, Raul Rivas, Pedro F Mateos, Eustoquio Martinezmolina, Jose Manuel Gonzalezbuitrago, Encarna VelazquezAbstract:Family Rhizobiaceae includes fast growing bacteria currently arranged into three genera, Rhizobium, Ensifer and Shinella, that contain pathogenic, symbiotic and saprophytic species. The identification of these species is not possible on the basis of physiological or biochemical traits and should be based on sequencing of several genes. Therefore alternative methods are necessary for rapid and reliable identification of members from family Rhizobiaceae. In this work we evaluated the suitability of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for this purpose. Firstly, we evaluated the capability of this methodology to differentiate among species of family Rhizobiaceae including those closely related and then we extended the database of MALDI Biotyper 2.0 including the type strains of 56 species from genera Rhizobium, Ensifer and Shinella. Secondly, we evaluated the identification potential of this methodology by using several strains isolated from different sources previously identified on the basis of their rrs, recA and atpD gene sequences. The 100% of these strains were correctly identified showing that MALDI-TOF MS is an excellent tool for identification of fast growing rhizobia applicable to large populations of isolates in ecological and taxonomic studies.
-
analysis of stable low molecular weight rna profiles of members of the family Rhizobiaceae
Applied and Environmental Microbiology, 1998Co-Authors: Encarna Velazquez, Pedro F Mateos, Jose Maria Cruzsanchez, Eustoquio MartinezmolinaAbstract:Staircase electrophoresis in polyacrylamide gels was used to analyze the stable low-molecular-weight (LMW) RNA profiles of 24 type strains belonging to the family Rhizobiaceae. This new electrophoretic technique results in good separation of the molecules forming the LMW RNA profiles. Differences in the number and distribution of the RNA bands in these profiles allowed us to identify differences among the 24 strains assayed. Species assignments based on LMW RNAs proved to be consistent with the established taxonomic classification. Analysis of the data obtained and the corresponding dendrograms revealed relationships between genera and species; these relationships were essentially the same as those obtained with other techniques, such as DNA hybridization and 16S rRNA sequencing. Use of the technique described here, with which it is possible to analyze a large number of strains in a short time, permits rapid identification of species belonging to the family Rhizobiaceae and should in the future facilitate biodiversity studies and detection of new species.
Miyoko Yanagi - One of the best experts on this subject based on the ideXlab platform.
-
phylogenetic analysis of the family Rhizobiaceae and related bacteria by sequencing of 16s rrna gene using pcr and dna sequencer
Fems Microbiology Letters, 1993Co-Authors: Miyoko Yanagi, Kazuhide YamasatoAbstract:The 16S rRNA gene sequences of 19 strains covering 97% of the molecules were determined for the members of the family Rhizobiaceae and related bacteria by PCR and DNA sequencer. The three biovars of Agrobacterium were located separately, whereas Agrobacterium rubi clustered with A. tumefaciens. Phylogenetic locations for the species of the genera Rhizobium, Sinorhizobium, Agrobacterium, Phylobacterium, Mycoplana (M. dimorpha), Ochrobactrum, Brucella and Rochalimaea (a rickettsia) were intermingled with each other with the similarity values higher than 92%. The family Rhizobiaceae should be redefined including the above-mentioned genera despite the ability for plant association and nitrogen fixation. Bradyrhizobium japonicum and Mycoplana bullata were far remote from the other species and should be excluded from this family.