The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Luis A Cordova - One of the best experts on this subject based on the ideXlab platform.
-
mutant ccl2 protein coating mitigates wear particle induced bone loss in a murine continuous polyethylene infusion model
Biomaterials, 2017Co-Authors: Akira Nabeshima, Luis A Cordova, Xinyi Jiang, Jukka Pajarinen, Tzuhua Lin, Florence Loi, Emmanuel Gibon, Eemeli Jamsen, Kensuke EgashiraAbstract:Wear particle-induced osteolysis limits the long-term survivorship of total joint replacement (TJR). Monocyte/macrophages are the key cells of this adverse reaction. Monocyte Chemoattractant Protein-1 (MCP-1/CCL2) is the most important chemokine regulating trafficking of monocyte/macrophages in particle-induced inflammation. 7ND recombinant protein is a mutant of CCL2 that inhibits CCL2 signaling. We have recently developed a layer-by-layer (LBL) coating platform on Implant surfaces that can release biologically active 7ND. In this study, we investigated the effect of 7ND on wear particle-induced bone loss using the murine continuous polyethylene (PE) particle infusion model with 7ND coating of a titanium rod as a local drug delivery device. PE particles were infused into hollow titanium rods with or without 7ND coating Implanted in the distal femur for 4 weeks. Specific groups were also injected with RAW 264.7 as the reporter macrophages. Wear particle-induced bone loss and the effects of 7ND were evaluated by microCT, immunohistochemical staining, and bioluminescence imaging. Local delivery of 7ND using the LBL coating decreased systemic macrophage recruitment, the number of osteoclasts and wear particle-induced bone loss. The development of a novel Orthopaedic Implant coating with anti-CCL2 protein may be a promising strategy to mitigate peri-prosthetic osteolysis.
-
Orthopaedic Implant failure aseptic Implant loosening the contribution and future challenges of mouse models in translational research
Clinical Science, 2014Co-Authors: Luis A Cordova, Verena Stresing, Berengere Gobin, Philippe RossetAbstract:Aseptic loosening as a result of wear debris is considered to be the main cause of long-term Implant failure in Orthopaedic surgery and improved biomaterials for bearing surfaces decreases significantly the release of micrometric wear particles. Increasingly, in-depth knowledge of osteoimmunology highlights the role of nanoparticles and ions released from some of these new bearing couples, opening up a new era in the comprehension of aseptic loosening. Mouse models have been essential in the progress made in the early comprehension of pathophysiology and in testing new therapeutic agents for particle-induced osteolysis. However, despite this encouraging progress, there is still no valid clinical alternative to revision surgery. The present review provides an update of the most commonly used bearing couples, the current concepts regarding particle-cell interactions and the approaches used to study the biology of periprosthetic osteolysis. It also discusses the contribution and future challenges of mouse models for successful translation of the preclinical progress into clinical applications.
F J Monteiro - One of the best experts on this subject based on the ideXlab platform.
-
lytic bacteriophages against multidrug resistant staphylococcus aureus enterococcus faecalis and escherichia coli isolates from Orthopaedic Implant associated infections
International Journal of Antimicrobial Agents, 2019Co-Authors: Joana Barros, Luis Daniel Rodrigues Melo, Patricia Poeta, Gilberto Igrejas, Maria Pia Ferraz, Joana Azeredo, F J MonteiroAbstract:Orthopaedic Implant-associated infections are a devastating complication of Orthopaedic surgery with a significant impact on patients and healthcare systems. The aims of this work were to describe the patterns of antimicrobial resistance, pathogenicity and virulence of clinical bacterial isolates from Orthopaedic Implant-associated infections and to further isolate and characterise bacteriophages that are efficient in controlling these bacteria. Staphylococcus aureus, Enterococcus faecalis and Escherichia coli isolated from Orthopaedic infections showed multiresistance patterns to the most frequently used antibiotics in clinical settings. The presence of mobile genetic elements (mecA, Tn916/Tn1545 and intl1) and virulence determinants (icaB, cna, hlb, cylLs, cylM, agg, gelE, fsr and fimA) highlighted the pathogenicity of these isolates. Moreover, the isolates belonged to clonal complexes associated with the acquisition of pathogenicity islands and antimicrobial resistance genes by recombination and horizontal gene transfer. Bacteriophages vB_SauM_LM12, vB_EfaS_LM99 and vB_EcoM_JB75 were characterised and their ability to infect clinical isolates of S. aureus, E. faecalis and E. coli, respectively, was assessed. Morphological and genomic analyses revealed that vB_EfaS_LM99 and vB_EcoM_JB75 belong to the Siphoviridae and Myoviridae families, respectively, and no genes associated with lysogeny were found. The bacteriophages showed low latent periods, high burst sizes, broad host ranges and tolerance to several environmental conditions. Moreover, they showed high efficiency and specificity to infect and reduce clinical bacteria, including methicillin-resistant S. aureus and vancomycin-resistant enterococci. Therefore, the results obtained suggest that the bacteriophages used in this work are a promising approach to control these pathogens involved in Orthopaedic Implant-associated infections.
Philippe Rosset - One of the best experts on this subject based on the ideXlab platform.
-
Orthopaedic Implant failure aseptic Implant loosening the contribution and future challenges of mouse models in translational research
Clinical Science, 2014Co-Authors: Luis A Cordova, Verena Stresing, Berengere Gobin, Philippe RossetAbstract:Aseptic loosening as a result of wear debris is considered to be the main cause of long-term Implant failure in Orthopaedic surgery and improved biomaterials for bearing surfaces decreases significantly the release of micrometric wear particles. Increasingly, in-depth knowledge of osteoimmunology highlights the role of nanoparticles and ions released from some of these new bearing couples, opening up a new era in the comprehension of aseptic loosening. Mouse models have been essential in the progress made in the early comprehension of pathophysiology and in testing new therapeutic agents for particle-induced osteolysis. However, despite this encouraging progress, there is still no valid clinical alternative to revision surgery. The present review provides an update of the most commonly used bearing couples, the current concepts regarding particle-cell interactions and the approaches used to study the biology of periprosthetic osteolysis. It also discusses the contribution and future challenges of mouse models for successful translation of the preclinical progress into clinical applications.
Lucio Montanaro - One of the best experts on this subject based on the ideXlab platform.
-
the presence of both bone sialoprotein binding protein gene and collagen adhesin gene as a typical virulence trait of the major epidemic cluster in isolates from orthopedic Implant infections
Biomaterials, 2009Co-Authors: Davide Campoccia, Pietro Speziale, Stefano Ravaioli, Ilaria Cangini, Simonetta Rindi, Valter Pirini, Lucio Montanaro, Carla Renata ArciolaAbstract:Staphylococcus aureus is a major, highly clonal, pathogen causing Implant infections. This study aimed at investigating the diverse distribution of bacterial adhesins in most prevalent S. aureus strain types causing Orthopaedic Implant infections. 200 S. aureus isolates, categorized into ribogroups by automated ribotyping, i.e. rDNA restriction fragment length polymorphism analysis, were screened for the presence of a panel of adhesins genes. Within the collection of isolates, automated ribotyping detected 98 distinct ribogroups. For many ribogroups, characteristic tandem genes arrangements could be identified. In the predominant S. aureus cluster, enlisting 27 isolates, the bbp gene encoding bone sialoprotein-binding protein appeared a typical virulence trait, found in 93% of the isolates. Conversely, the bbp gene was identified in just 10% of the remaining isolates of the collection. In this cluster, co-presence of bbp with the cna gene encoding collagen adhesin was a pattern consistently observed. These findings indicate a crucial role of both these adhesins, able to bind the most abundant bone proteins, in the pathogenesis of Orthopaedic Implant infections, there where biomaterials interface bone tissues. This study suggests that specific adhesins may synergistically act in the onset of Implant infections and that anti-adhesin strategies should be targeted to adhesins conjointly present.
-
strong biofilm production antibiotic multi resistance and high gele expression in epidemic clones of enterococcus faecalis from Orthopaedic Implant infections
Biomaterials, 2008Co-Authors: Carla Renata Arciola, Davide Campoccia, Valter Pirini, Lucilla Baldassarri, Roberta Creti, Johannes Huebner, Lucio MontanaroAbstract:Enterococcus faecalis is an opportunistic pathogen, which today represents one of the leading aetiologic agents of nosocomial infections and, increasingly, of Implant infections. Here, in a collection of 43 E. faecalis isolated from Implant Orthopaedic infections, virulence-related phenotypes (biofilm and gelatinase production) and genotypes (gelE and esp) were studied to characterize epidemic clones identified and grouped by ribotyping. The presence of the esp gene and a marked and steady biofilm formation ability appeared to be the features associated with the clonal spreading, as well as a conspicuous gelatinase production, whereas the simple presence of gelE appeared non-specific of the epidemic clones. Antibiotic multi-resistance and strong biofilm production abilities together with a high phenotypic expression of gelatinase are an important equipment of E. faecalis to colonize peri-prosthesis tissues and to spread out as causative agents of Implant Orthopaedic infections.
-
antibiotic resistance in exopolysaccharide forming staphylococcus epidermidis clinical isolates from Orthopaedic Implant infections
Biomaterials, 2005Co-Authors: Carla Renata Arciola, Davide Campoccia, Pietro Speziale, Valter Pirini, S Gamberini, M E Donati, Livia Visai, Lucio MontanaroAbstract:Abstract The opportunistic pathogen Staphylococcus epidermidis is able to produce biofilm and to frequently cause Implant infections. In recent years, it has also exhibited an increasing antimicrobial drug resistance. Here, the resistance to a panel of 16 different antibiotics in 342 clinical strains of S. epidermidis from Orthopaedic Implant infections has been investigated. The isolates were pheno- and genotyped for extracellular polysaccharide production, relevant to staphylococcal biofilm formation, in order to ascertain possible associations with antibiotic resistance. Approximately 10% of the isolates were found to be sensitive to all screened antibiotics. In all, 37–38% were resistant to β-lactams such as oxacillin and imipenem, while the resistance to penicillin, ampicillin, cefazolin, cefamandole, was consistently observed in over 80% of the strains. Erythromycin- and clindamycin- resistant strains were approximately 41% and 16%, respectively. Of the isolates, 10% was resistant to chloramphenicol, 23% to sulfamethoxazole and 26% to ciprofloxacin. Resistance to vancomycin was never observed. Interestingly, exopolysaccharide-producing strains exhibited a significantly higher prevalence in the resistance to the four aminoglycosides (gentamicin, amikacin, netilmicin, tobramycin), to sulfamethoxazole and to ciprofloxacin with respect to non-producing isolates. Moreover, multiple resistance to antibiotics was more frequent among exopolysaccharide-forming strains.
Joana Barros - One of the best experts on this subject based on the ideXlab platform.
-
lytic bacteriophages against multidrug resistant staphylococcus aureus enterococcus faecalis and escherichia coli isolates from Orthopaedic Implant associated infections
International Journal of Antimicrobial Agents, 2019Co-Authors: Joana Barros, Luis Daniel Rodrigues Melo, Patricia Poeta, Gilberto Igrejas, Maria Pia Ferraz, Joana Azeredo, F J MonteiroAbstract:Orthopaedic Implant-associated infections are a devastating complication of Orthopaedic surgery with a significant impact on patients and healthcare systems. The aims of this work were to describe the patterns of antimicrobial resistance, pathogenicity and virulence of clinical bacterial isolates from Orthopaedic Implant-associated infections and to further isolate and characterise bacteriophages that are efficient in controlling these bacteria. Staphylococcus aureus, Enterococcus faecalis and Escherichia coli isolated from Orthopaedic infections showed multiresistance patterns to the most frequently used antibiotics in clinical settings. The presence of mobile genetic elements (mecA, Tn916/Tn1545 and intl1) and virulence determinants (icaB, cna, hlb, cylLs, cylM, agg, gelE, fsr and fimA) highlighted the pathogenicity of these isolates. Moreover, the isolates belonged to clonal complexes associated with the acquisition of pathogenicity islands and antimicrobial resistance genes by recombination and horizontal gene transfer. Bacteriophages vB_SauM_LM12, vB_EfaS_LM99 and vB_EcoM_JB75 were characterised and their ability to infect clinical isolates of S. aureus, E. faecalis and E. coli, respectively, was assessed. Morphological and genomic analyses revealed that vB_EfaS_LM99 and vB_EcoM_JB75 belong to the Siphoviridae and Myoviridae families, respectively, and no genes associated with lysogeny were found. The bacteriophages showed low latent periods, high burst sizes, broad host ranges and tolerance to several environmental conditions. Moreover, they showed high efficiency and specificity to infect and reduce clinical bacteria, including methicillin-resistant S. aureus and vancomycin-resistant enterococci. Therefore, the results obtained suggest that the bacteriophages used in this work are a promising approach to control these pathogens involved in Orthopaedic Implant-associated infections.
