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Snehasis Jana - One of the best experts on this subject based on the ideXlab platform.
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assessment of biofield energy healing based vitamin d3 effects on bone health parameters using human Osteoblast Cell Line mg 63
viXra, 2019Co-Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Snehasis JanaAbstract:Poor bone health is the primary health issue, which leads to significant health problems, stress and worsening the patients' quality of life. The potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 as a test item (TI) and DMEM on MG-63 Cells was investigated. The test items were treated with The Trivedi Effect® by Mahendra Kumar Trivedi and divided as Biofield Energy Treated (BT) and untreated (UT) test items. An increase in ALP activity, collagen levels, and bone mineralization was considered as the biomarker for bone health. MTT data showed that the test samples observed nontoxic in the tested concentrations. The level of ALP was significantly increased by 832.9% and 209.4% in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively at 10 µg/mL, while 222.9% increase in the BT-DMEM+BT-TI at 1 µg/mL compared to the untreated group. Collagen was significantly increased by 487.7% and 544.5% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100 µg/mL, while 116.2% at 1 µg/mL in UT-DMEM+BT-TI compared to the untreated group. Moreover, the percent of bone mineralization was significantly increased in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups by 344.9% and 149.7%, respectively at 50 µg/mL, while 183.6% in the BT-DMEM+BT-TI group at 100 µg/mL compared to the untreated group. Thus, the role of Biofield Energy Treated vitamin D3 and DMEM in order to control Osteoblast function and its direct effects on bone mineralization can be used to improve bone disorders.
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in vitro effects of biofield energy treated vitamin d3 supplementation on bone formation by Osteoblast Cell Line mg 63
Journal of Orthopedic Research and Therapy, 2018Co-Authors: Dahryn Trivedi, Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Snehasis JanaAbstract:Inadequate intake of vitamin D leads to hormonal imbalance, aging, decreased calcium absorption, and bone loss. Present study aimed to evaluate potential of The Trivedi Effect®- Biofield Energy Healing Treatment on vitamin D3 as Test Item (TI) and DMEM to improve bone health in MG-63 Cells. One part of each samples was received Consciousness Energy Healing Treatment by Dahryn Trivedi and those samples were labeled as Biofield Energy Treated (BT) samples, while other parts of each sample were denoted as untreated TI (UT-TI). Cell viability assay (MTT) found test items were safe and nontoxic in the tested concentrations. ALP was considerably improved by 753.3%, 1173.3%, and 424.4% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 0.1 µg/mL compared to untreated. Collagen was significantly increased by 454.7% and 96.2% in BT-DMEM+UT-TI and BT-DMEM+BT-TI, respectively at 1 µg/mL, while 202.4% (at 50 µg/mL) increased collagen in UT-DMEM+BT-TI compared to untreated. Moreover, percent of bone mineralization was significantly increased in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI by 301.4% (at 50 µg/mL), 143.3% (at 50 µg/mL), and 178.0% (at 100 µg/mL) respectively, compared to untreated. Thus, Biofield Energy Treated vitamin D3 and Biofield Energy Treated DMEM were found safe and to have remarkably improved the bone health parameters, which could be a powerful alternative nutraceutical supplement to combat against various bone-related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.
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assessment of biofield energy healing based vitamin d3 effects on bone health parameters using human Osteoblast Cell Line mg 63
International Journal of Nutrition, 2018Co-Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Snehasis JanaAbstract:Author(s): Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Jana, Snehasis | Abstract: Poor bone health is the primary health issue, which leads to significant health problems, stress and worsening the patients' quality of life. The potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 as a test item (TI) and DMEM on MG-63 Cells was investigated. The test items were treated with The Trivedi Effect® by Mahendra Kumar Trivedi and divided as Biofield Energy Treated (BT) and untreated (UT) test items. An increase in ALP activity, collagen levels, and bone mineralization was considered as the biomarker for bone health. MTT data showed that the test samples observed nontoxic in the tested concentrations. The level of ALP was significantly increased by 832.9% and 209.4% in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively at 10 µg/mL, while 222.9% increase in the BT-DMEM+BT-TI at 1 µg/mL compared to the untreated group. Collagen was significantly increased by 487.7% and 544.5% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100 µg/mL, while 116.2% at 1 µg/mL in UT-DMEM+BT-TI compared to the untreated group. Moreover, the percent of bone mineralization was significantly increased in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups by 344.9% and 149.7%, respectively at 50 µg/mL, while 183.6% in the BT-DMEM+BT-TI group at 100 µg/mL compared to the untreated group. Thus, the role of Biofield Energy Treated vitamin D3 and DMEM in order to control Osteoblast function and its direct effects on bone mineralization can be used to improve bone disorders.
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impact of biofield energy healing treated vitamin d3 on human Osteoblast Cell Line mg 63 for bone health
viXra, 2018Co-Authors: William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis JanaAbstract:Bone disorders are largely associated with increased morbidity, mortality, and substantial economic and health costs. Vitamin D play an important role for calcium absorption and bone mineralization, which can improve the patients' quality of life. The current study aimed to evaluate the potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 and DMEM as test item (TI) on bone Cell differentiation using human Osteoblast Cell Line (MG-63, Osteosarcoma). Bone health biomarkers such as alkaLine phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. The test items were treated with The Trivedi Effect® by William Dean Plikerd and divided as Biofield Energy Treated (BT) and untreated (UT) test items. Cell viability using MTT data showed that the test items were found to be safe. ALP level was significantly increased by 346.4% (at 50 µg/mL), 375.3% (at 100 µg/mL), and 343.2% (at 100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Collagen content was significantly increased by 336.2% and 237.2% in the UT-DMEM+BT-TI at 10 and 50 µg/mL, respectively while 399.3% (10 µg/mL) and 200% (0.1 µg/mL) increased collagen in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively. Moreover, the percent of bone mineralization was significantly increased in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups by 416.9% (10 µg/mL), 460.7% (0.1 µg/mL), and 441.7% (10 µg/mL) respectively as compared with the untreated test item and DMEM group. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the Osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.
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regulation of bone health parameters after treatment with biofield energy healing based vitamin d3 on human Osteoblast Cell Line mg 63
viXra, 2018Co-Authors: Victoria L Vannes, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis JanaAbstract:Bone disorders dramatically affecting the functional status of many individuals, which are suffering from bone diseases such as fractures, significant pain and height loss, disability to stand up, and walk. Vitamin D play an important role to improve the patients' quality of life with respect to bone disorders. The current study aimed to evaluate the potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 and DMEM as test item (TI) on bone Cell differentiation using human Osteoblast Cell Line (MG-63, Osteosarcoma). Bone health biomarkers such as alkaLine phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. The test items were treated with The Trivedi Effect® by Victoria Lee Vannes and divided as Biofield Energy Treated (BT) and untreated (UT) test items. Cell viability using MTT data showed that the test items were found to be safe. ALP level was significantly increased by 114.3%, 304.8%, and 314.3% at 0.1 µg/mL in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Collagen content was significantly increased by 82.5%, 138.4%, and 100.8% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI, while 120.6% and 64.6% increased collagen at 0.1 and 1 µg/mL in BT-DMEM+UT-TI group and 261.9%, 179.8%, and 116.0% increased collagen in BT-DMEM+BT-TI group at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 261.2% and 239.9% at 1 µg/mL UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively as compared with the untreated group. However, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 324.5% at 50 µg/mL. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the Osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.
Gerald J Atkins - One of the best experts on this subject based on the ideXlab platform.
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comparison of the biological effects of exogenous and endogenous 1 25 dihydroxyvitamin d3 on the mature Osteoblast Cell Line mlo a5
The Journal of Steroid Biochemistry and Molecular Biology, 2016Co-Authors: Dongqing Yang, Andrew G Turner, Paul H Anderson, Howard A Morris, Gerald J AtkinsAbstract:Clinical and animal data indicate that serum 25-hydroxyvitamin D3 (25D) exerts an anabolic effect on bone while serum 1α,25-dihydroxyvitamin D3 (1,25D) stimulates bone mineral loss, although the mechanism responsible for these divergent actions is unknown. Biological effects of 25D on bone Cells are dependent on the local conversion to 1,25D by the 25-hydroxyvitamin D-1α-hydroxylase enzyme, CYP27B1. Therefore, identification of possible differential activities of locally produced and exogenously supplied 1,25D in bone is likely to be informative for guiding optimal administration of vitamin D supplements for bone health. The mature Osteoblastic Cell Line MLO-A5 expresses both the vitamin D receptor (Vdr) and Cyp27b1, and therefore is a suitable model for comparing the activities of 1,25D arising from these sources. Biologically, exogenous and endogenous sources of 1,25D have similar effects on proliferation, mineralisation and induction of a range of genes by MLO-A5 Osteoblasts under osteogenic conditions although endogenous 1,25D levels are markedly lower than exogenous levels. Significant differences of pharmacokinetics and pharmacodynamics of 1,25D are evident between these two sources particularly in terms of modulating gene expression for Cyp24a1 and other genes largely expressed by embedded Osteoblasts/osteocytes suggesting that endogenously synthesised 1,25D is more efficiently utilised by the differentiating Osteoblast.
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1 25 dihydroxyvitamin d3 and extraCellular calcium promote mineral deposition via npp1 activity in a mature Osteoblast Cell Line mlo a5
Molecular and Cellular Endocrinology, 2015Co-Authors: Dongqing Yang, Andrew G Turner, Asiri R Wijenayaka, Paul H Anderson, Howard A Morris, Gerald J AtkinsAbstract:While vitamin D supplementation is common, the anabolic mechanisms that improve bone status are poorly understood. Under standard mineralising conditions including media ionised calcium of 1.1 mM, 1,25-dihydroxyvitamin D3 (1,25D) enhanced differentiation and mineral deposition by the mature Osteoblast/pre-osteocyte Cell Line, MLO-A5. This effect was markedly increased with a higher ionised calcium level (1.5 mM). Gene expression analyses revealed that 1,25D-induced mineral deposition was associated with induction of Enpp1 mRNA, coding for nucleotide pyrophosphatase phosphodiesterase 1 (NPP1) and NPP1 protein levels. Since MLO-A5 Cells express abundant alkaLine phosphatase that was not further modified by 1,25D treatment or exposure to increased calcium, this finding suggested that the NPP1 production of pyrophosphate (PPi) may provide alkaLine phosphatase with substrate for the generation of inorganic phosphate (Pi). Consistent with this, co-treatment with Enpp1 siRNA or a NPP1 inhibitor, PPADS, abrogated 1,25D-induced mineral deposition. These data demonstrate that 1,25D stimulates Osteoblast differentiation and mineral deposition, and interacts with the extraCellular calcium concentration. 1,25D regulates Enpp1 expression, which presumably, in the context of adequate tissue non-specific alkaLine phosphatase activity, provides Pi to stimulate mineralisation. Our findings suggest a mechanism by which vitamin D with adequate dietary calcium can improve bone mineral status.
Li-an Wu - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of a mouse floxed bmp2 Osteoblast Cell Line that retains Osteoblast genotype and phenotype
Cell and Tissue Research, 2011Co-Authors: Lynn Wang, Yan Dong Mu, Junsheng Feng, Stephen E Harris, Andrew Baker, Li-an Wu, Kevin J. Donly, Mary MacdougallAbstract:Bone morphogenetic protein 2 (Bmp2) is essential for Osteoblast differentiation and osteogenesis. Generation of floxed Bmp2 Osteoblast Cell Lines is a valuable tool for studying the effects of Bmp2 on Osteoblast differentiation and its signaling pathways during skeletal metabolism. Due to relatively limited sources of primary Osteoblasts, we have developed Cell Lines that serve as good surrogate models for the study of Osteoblast Cell differentiation and bone mineralization. In this study, we established and characterized immortalized mouse floxed Bmp2 Osteoblast Cell Lines. Primary mouse floxed Bmp2 Osteoblasts were transfected with pSV3-neo and clonally selected. These transfected Cells were verified by PCR and immunohistochemistry. To determine the genotype and phenotype of the immortalized Cells, Cell morphology, proliferation, differentiation and mineralization were analyzed. Also, expression of Osteoblast-related gene markers including Runx2, Osx, ATF4, Dlx3, bone sialoprotein, dentin matrix protein 1, osteonectin, osteocalcin and osteopontin were examined by quantitative RT-PCR and immunohistochemistry. These results showed that immortalized floxed Bmp2 Osteoblasts had a higher proliferation rate but preserved their genotypic and phenotypic characteristics similar to the primary Cells. Thus, we, for the first time, describe the development of immortalized mouse floxed Bmp2 Osteoblast Cell Lines and present a useful model to study Osteoblast biology mediated by BMP2 and its downstream signaling transduction pathways.
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immortalization and characterization of mouse floxed bmp2 4 Osteoblasts
Biochemical and Biophysical Research Communications, 2009Co-Authors: Iris Ortizgonzalez, Wuchen Yang, Mary Macdougall, Li-an Wu, Guohua Yuan, Guobin Yang, Kevin J. DonlyAbstract:Generation of a floxed Bmp2/4 Osteoblast Cell Line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on Osteoblast differentiation as well as relevant molecular events. In this study, primary floxed Bmp2/4 mouse Osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected Cells, morphology, proliferation and mineralization were analyzed, expression of Cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected floxed Bmp2/4 Osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary Cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse floxed Bmp2/4 Osteoblast Cell Line.
Dongqing Yang - One of the best experts on this subject based on the ideXlab platform.
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comparison of the biological effects of exogenous and endogenous 1 25 dihydroxyvitamin d3 on the mature Osteoblast Cell Line mlo a5
The Journal of Steroid Biochemistry and Molecular Biology, 2016Co-Authors: Dongqing Yang, Andrew G Turner, Paul H Anderson, Howard A Morris, Gerald J AtkinsAbstract:Clinical and animal data indicate that serum 25-hydroxyvitamin D3 (25D) exerts an anabolic effect on bone while serum 1α,25-dihydroxyvitamin D3 (1,25D) stimulates bone mineral loss, although the mechanism responsible for these divergent actions is unknown. Biological effects of 25D on bone Cells are dependent on the local conversion to 1,25D by the 25-hydroxyvitamin D-1α-hydroxylase enzyme, CYP27B1. Therefore, identification of possible differential activities of locally produced and exogenously supplied 1,25D in bone is likely to be informative for guiding optimal administration of vitamin D supplements for bone health. The mature Osteoblastic Cell Line MLO-A5 expresses both the vitamin D receptor (Vdr) and Cyp27b1, and therefore is a suitable model for comparing the activities of 1,25D arising from these sources. Biologically, exogenous and endogenous sources of 1,25D have similar effects on proliferation, mineralisation and induction of a range of genes by MLO-A5 Osteoblasts under osteogenic conditions although endogenous 1,25D levels are markedly lower than exogenous levels. Significant differences of pharmacokinetics and pharmacodynamics of 1,25D are evident between these two sources particularly in terms of modulating gene expression for Cyp24a1 and other genes largely expressed by embedded Osteoblasts/osteocytes suggesting that endogenously synthesised 1,25D is more efficiently utilised by the differentiating Osteoblast.
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1 25 dihydroxyvitamin d3 and extraCellular calcium promote mineral deposition via npp1 activity in a mature Osteoblast Cell Line mlo a5
Molecular and Cellular Endocrinology, 2015Co-Authors: Dongqing Yang, Andrew G Turner, Asiri R Wijenayaka, Paul H Anderson, Howard A Morris, Gerald J AtkinsAbstract:While vitamin D supplementation is common, the anabolic mechanisms that improve bone status are poorly understood. Under standard mineralising conditions including media ionised calcium of 1.1 mM, 1,25-dihydroxyvitamin D3 (1,25D) enhanced differentiation and mineral deposition by the mature Osteoblast/pre-osteocyte Cell Line, MLO-A5. This effect was markedly increased with a higher ionised calcium level (1.5 mM). Gene expression analyses revealed that 1,25D-induced mineral deposition was associated with induction of Enpp1 mRNA, coding for nucleotide pyrophosphatase phosphodiesterase 1 (NPP1) and NPP1 protein levels. Since MLO-A5 Cells express abundant alkaLine phosphatase that was not further modified by 1,25D treatment or exposure to increased calcium, this finding suggested that the NPP1 production of pyrophosphate (PPi) may provide alkaLine phosphatase with substrate for the generation of inorganic phosphate (Pi). Consistent with this, co-treatment with Enpp1 siRNA or a NPP1 inhibitor, PPADS, abrogated 1,25D-induced mineral deposition. These data demonstrate that 1,25D stimulates Osteoblast differentiation and mineral deposition, and interacts with the extraCellular calcium concentration. 1,25D regulates Enpp1 expression, which presumably, in the context of adequate tissue non-specific alkaLine phosphatase activity, provides Pi to stimulate mineralisation. Our findings suggest a mechanism by which vitamin D with adequate dietary calcium can improve bone mineral status.
Mahendra Kumar Trivedi - One of the best experts on this subject based on the ideXlab platform.
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assessment of biofield energy healing based vitamin d3 effects on bone health parameters using human Osteoblast Cell Line mg 63
viXra, 2019Co-Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Snehasis JanaAbstract:Poor bone health is the primary health issue, which leads to significant health problems, stress and worsening the patients' quality of life. The potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 as a test item (TI) and DMEM on MG-63 Cells was investigated. The test items were treated with The Trivedi Effect® by Mahendra Kumar Trivedi and divided as Biofield Energy Treated (BT) and untreated (UT) test items. An increase in ALP activity, collagen levels, and bone mineralization was considered as the biomarker for bone health. MTT data showed that the test samples observed nontoxic in the tested concentrations. The level of ALP was significantly increased by 832.9% and 209.4% in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively at 10 µg/mL, while 222.9% increase in the BT-DMEM+BT-TI at 1 µg/mL compared to the untreated group. Collagen was significantly increased by 487.7% and 544.5% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100 µg/mL, while 116.2% at 1 µg/mL in UT-DMEM+BT-TI compared to the untreated group. Moreover, the percent of bone mineralization was significantly increased in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups by 344.9% and 149.7%, respectively at 50 µg/mL, while 183.6% in the BT-DMEM+BT-TI group at 100 µg/mL compared to the untreated group. Thus, the role of Biofield Energy Treated vitamin D3 and DMEM in order to control Osteoblast function and its direct effects on bone mineralization can be used to improve bone disorders.
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in vitro effects of biofield energy treated vitamin d3 supplementation on bone formation by Osteoblast Cell Line mg 63
Journal of Orthopedic Research and Therapy, 2018Co-Authors: Dahryn Trivedi, Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Snehasis JanaAbstract:Inadequate intake of vitamin D leads to hormonal imbalance, aging, decreased calcium absorption, and bone loss. Present study aimed to evaluate potential of The Trivedi Effect®- Biofield Energy Healing Treatment on vitamin D3 as Test Item (TI) and DMEM to improve bone health in MG-63 Cells. One part of each samples was received Consciousness Energy Healing Treatment by Dahryn Trivedi and those samples were labeled as Biofield Energy Treated (BT) samples, while other parts of each sample were denoted as untreated TI (UT-TI). Cell viability assay (MTT) found test items were safe and nontoxic in the tested concentrations. ALP was considerably improved by 753.3%, 1173.3%, and 424.4% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 0.1 µg/mL compared to untreated. Collagen was significantly increased by 454.7% and 96.2% in BT-DMEM+UT-TI and BT-DMEM+BT-TI, respectively at 1 µg/mL, while 202.4% (at 50 µg/mL) increased collagen in UT-DMEM+BT-TI compared to untreated. Moreover, percent of bone mineralization was significantly increased in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI by 301.4% (at 50 µg/mL), 143.3% (at 50 µg/mL), and 178.0% (at 100 µg/mL) respectively, compared to untreated. Thus, Biofield Energy Treated vitamin D3 and Biofield Energy Treated DMEM were found safe and to have remarkably improved the bone health parameters, which could be a powerful alternative nutraceutical supplement to combat against various bone-related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.
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assessment of biofield energy healing based vitamin d3 effects on bone health parameters using human Osteoblast Cell Line mg 63
International Journal of Nutrition, 2018Co-Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Snehasis JanaAbstract:Author(s): Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Jana, Snehasis | Abstract: Poor bone health is the primary health issue, which leads to significant health problems, stress and worsening the patients' quality of life. The potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 as a test item (TI) and DMEM on MG-63 Cells was investigated. The test items were treated with The Trivedi Effect® by Mahendra Kumar Trivedi and divided as Biofield Energy Treated (BT) and untreated (UT) test items. An increase in ALP activity, collagen levels, and bone mineralization was considered as the biomarker for bone health. MTT data showed that the test samples observed nontoxic in the tested concentrations. The level of ALP was significantly increased by 832.9% and 209.4% in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively at 10 µg/mL, while 222.9% increase in the BT-DMEM+BT-TI at 1 µg/mL compared to the untreated group. Collagen was significantly increased by 487.7% and 544.5% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100 µg/mL, while 116.2% at 1 µg/mL in UT-DMEM+BT-TI compared to the untreated group. Moreover, the percent of bone mineralization was significantly increased in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups by 344.9% and 149.7%, respectively at 50 µg/mL, while 183.6% in the BT-DMEM+BT-TI group at 100 µg/mL compared to the untreated group. Thus, the role of Biofield Energy Treated vitamin D3 and DMEM in order to control Osteoblast function and its direct effects on bone mineralization can be used to improve bone disorders.
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impact of biofield energy healing treated vitamin d3 on human Osteoblast Cell Line mg 63 for bone health
viXra, 2018Co-Authors: William Dean Plikerd, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis JanaAbstract:Bone disorders are largely associated with increased morbidity, mortality, and substantial economic and health costs. Vitamin D play an important role for calcium absorption and bone mineralization, which can improve the patients' quality of life. The current study aimed to evaluate the potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 and DMEM as test item (TI) on bone Cell differentiation using human Osteoblast Cell Line (MG-63, Osteosarcoma). Bone health biomarkers such as alkaLine phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. The test items were treated with The Trivedi Effect® by William Dean Plikerd and divided as Biofield Energy Treated (BT) and untreated (UT) test items. Cell viability using MTT data showed that the test items were found to be safe. ALP level was significantly increased by 346.4% (at 50 µg/mL), 375.3% (at 100 µg/mL), and 343.2% (at 100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Collagen content was significantly increased by 336.2% and 237.2% in the UT-DMEM+BT-TI at 10 and 50 µg/mL, respectively while 399.3% (10 µg/mL) and 200% (0.1 µg/mL) increased collagen in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively. Moreover, the percent of bone mineralization was significantly increased in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups by 416.9% (10 µg/mL), 460.7% (0.1 µg/mL), and 441.7% (10 µg/mL) respectively as compared with the untreated test item and DMEM group. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the Osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.
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regulation of bone health parameters after treatment with biofield energy healing based vitamin d3 on human Osteoblast Cell Line mg 63
viXra, 2018Co-Authors: Victoria L Vannes, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis JanaAbstract:Bone disorders dramatically affecting the functional status of many individuals, which are suffering from bone diseases such as fractures, significant pain and height loss, disability to stand up, and walk. Vitamin D play an important role to improve the patients' quality of life with respect to bone disorders. The current study aimed to evaluate the potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 and DMEM as test item (TI) on bone Cell differentiation using human Osteoblast Cell Line (MG-63, Osteosarcoma). Bone health biomarkers such as alkaLine phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. The test items were treated with The Trivedi Effect® by Victoria Lee Vannes and divided as Biofield Energy Treated (BT) and untreated (UT) test items. Cell viability using MTT data showed that the test items were found to be safe. ALP level was significantly increased by 114.3%, 304.8%, and 314.3% at 0.1 µg/mL in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Collagen content was significantly increased by 82.5%, 138.4%, and 100.8% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI, while 120.6% and 64.6% increased collagen at 0.1 and 1 µg/mL in BT-DMEM+UT-TI group and 261.9%, 179.8%, and 116.0% increased collagen in BT-DMEM+BT-TI group at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 261.2% and 239.9% at 1 µg/mL UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively as compared with the untreated group. However, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 324.5% at 50 µg/mL. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the Osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.
