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Réjane Streiff - One of the best experts on this subject based on the ideXlab platform.
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Exploration of viral communities associated with lepidoptera : A study of Thaumetopoea pityocampa (Notodontidae) and the European corn borer species complex Ostrinia nubilalis / O. scapulalis
2018Co-Authors: Franck Dorkeld, Bernhard Gschloessl, Carole Kerdelhué, Réjane Streiff, Bernard Duvic, Marie Helene OgliastroAbstract:Exploration of viral communities associated with lepidoptera : A study of Thaumetopoea pityocampa (Notodontidae) and the European corn borer species complex Ostrinia nubilalis / O. scapulalis. 10. International Workshop on the Molecular Biology and Genetics of the Lepidoptera
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de novo transcriptomic resources for two sibling species of moths Ostrinia nubilalis and o scapulalis
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Emmanuelle Beyne, Philippe Audiot, Denis Bourguet, Réjane StreiffAbstract:Background This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics.
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De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis.
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Emmanuelle Beyne, Philippe Audiot, Denis Bourguet, Réjane StreiffAbstract:Background: This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics. Results: Based on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes. Conclusions: This data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes. Keywords: NGS, 454 sequencing, Tanscriptome, Moth, European corn borer, Adzuki bean borer, Sibling species, Ostrinia nubilalis, Ostrinia scapulalis
Bernhard Gschloessl - One of the best experts on this subject based on the ideXlab platform.
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Exploration of viral communities associated with lepidoptera : A study of Thaumetopoea pityocampa (Notodontidae) and the European corn borer species complex Ostrinia nubilalis / O. scapulalis
2018Co-Authors: Franck Dorkeld, Bernhard Gschloessl, Carole Kerdelhué, Réjane Streiff, Bernard Duvic, Marie Helene OgliastroAbstract:Exploration of viral communities associated with lepidoptera : A study of Thaumetopoea pityocampa (Notodontidae) and the European corn borer species complex Ostrinia nubilalis / O. scapulalis. 10. International Workshop on the Molecular Biology and Genetics of the Lepidoptera
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de novo transcriptomic resources for two sibling species of moths Ostrinia nubilalis and o scapulalis
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Emmanuelle Beyne, Philippe Audiot, Denis Bourguet, Réjane StreiffAbstract:Background This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics.
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De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis.
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Emmanuelle Beyne, Philippe Audiot, Denis Bourguet, Réjane StreiffAbstract:Background: This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics. Results: Based on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes. Conclusions: This data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes. Keywords: NGS, 454 sequencing, Tanscriptome, Moth, European corn borer, Adzuki bean borer, Sibling species, Ostrinia nubilalis, Ostrinia scapulalis
Emmanuelle Beyne - One of the best experts on this subject based on the ideXlab platform.
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de novo transcriptomic resources for two sibling species of moths Ostrinia nubilalis and o scapulalis
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Emmanuelle Beyne, Philippe Audiot, Denis Bourguet, Réjane StreiffAbstract:Background This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics.
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De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis.
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Emmanuelle Beyne, Philippe Audiot, Denis Bourguet, Réjane StreiffAbstract:Background: This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics. Results: Based on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes. Conclusions: This data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes. Keywords: NGS, 454 sequencing, Tanscriptome, Moth, European corn borer, Adzuki bean borer, Sibling species, Ostrinia nubilalis, Ostrinia scapulalis
Mihajlo B. Spasić - One of the best experts on this subject based on the ideXlab platform.
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Cold hardiness in Ostrinia nubilalis (Lepidoptera: Pyralidae): glycerol content, hexose monophosphate shunt activity, and antioxidative defense system
European Journal of Endocrinology, 2004Co-Authors: Bojana Stanic, Aleksandra Jovanovic-galovic, Duško Blagojević, Gordana Grubor-lajšić, Roger Worland, Mihajlo B. SpasićAbstract:Many insects in temperate regions overwinter in diapause, during which they are cold hardy. In these insects, one of the metabolic adaptations to the unfavorable environmental conditions is the synthesis of cryoprotectants/anhydroprotectants. The aim of this study was to investigate the connection between the antioxidative system and synthesis of cryoprotectants (mainly glycerol) in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae). At two periods during diapause (November and February), in three groups of insects (kept under field conditions; -12°C for two weeks; 8°C for two weeks), the activity of key enzymes of the antioxidative system and oxidative part of the hexose monophosphate shunt were measured: super- oxide dismutase, catalase, non selenium glutathione peroxidase, glutathione S-transferase, glutathione reductase, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, as well that of the antioxidative components: total glutathione and ascorbate, and dehydroascorbate reductase. There was a higher activity of antioxidative enzymes at the beginning of the dia- pause period (November) compared to late diapause (February), while glutathione and ascorbate were higher in February. Similarly, there was a lower activity of the hexose monophosphate shunt enzymes in February. Exposure of larvae to -12°C resulted in an ele- vation of hexose monophosphate shunt enzyme activity, especially in November. This was accompanied by a significant increase in glycerol content in February. Changes in ascorbate levels and dehydroascorbate reductase activity in both experimental groups (-12°C and 8°C) suggest a connection between the antioxidative system, metabolism during diapause and cold hardiness. Our results support the notion that antioxidative defense in larvae of Ostrinia nubilalis is closely connected with metabolic changes character- istic of diapause, mechanisms of cold hardiness involved in diapause and the maintenance of a stable redox state.
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Role of antioxidant defense during different stages of preadult life cycle in European corn borer (Ostrinia nubilalis, Hubn.): Diapause and metamorphosis.
Archives of insect biochemistry and physiology, 2004Co-Authors: Aleksandra Jovanovic-galovic, Duško Blagojević, Gordana Grubor-lajšić, Roger Worland, Mihajlo B. SpasićAbstract:Antioxidant enzymes, total glutathione (GSH), and ascorbic acid (ASA) were determined in whole body homogenates of nondiapausing larvae, diapausing larvae during the diapausing period (October, December, and February), and in pupae emerged from both diapausing and nondiapausing larvae of the European corn borer (Ostrinia nubilalis, Hubn., Lepidoptera: Pyralidae). The activities of catalase, selenium nondependent glutathione peroxidase (GPx), and glutathione-S-transferase (GST), as well as the content of GSH and ASA, were found to vary throughout the larval diapause. Compared to diapausing larvae, nondiapausing larvae were higher in levels of catalase, GPx, GST, and dehydroascorbate reductase (DHAR) activity. GSH content was also increased. However, nondiapausing larvae contained less ASA than diapausing ones. Pupae had higher GPx and GST activity and an increased ASA content compared to larvae. The pupae emerged from nondiapausing larvae had higher GST, glutathione reductase (GR), and DHAR activities, but lower GPx activity and ASA content than those emerged from diapausing larvae. Correlation analysis revealed differences in the way the antioxidant level is equilibrated for a particular stage and developmental pattern. The results suggest that cellular antioxidants are involved in both the protection of cells and the regulation of redox levels during the pre-adult stages of Ostrinia nubilalis. Arch. Insect Biochem. Physiol. 55:79-89, 2004.
Philippe Audiot - One of the best experts on this subject based on the ideXlab platform.
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de novo transcriptomic resources for two sibling species of moths Ostrinia nubilalis and o scapulalis
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Emmanuelle Beyne, Philippe Audiot, Denis Bourguet, Réjane StreiffAbstract:Background This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics.
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De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis.
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Emmanuelle Beyne, Philippe Audiot, Denis Bourguet, Réjane StreiffAbstract:Background: This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics. Results: Based on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes. Conclusions: This data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes. Keywords: NGS, 454 sequencing, Tanscriptome, Moth, European corn borer, Adzuki bean borer, Sibling species, Ostrinia nubilalis, Ostrinia scapulalis
