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Yukio Ishikawa - One of the best experts on this subject based on the ideXlab platform.
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Molecular Bases for the Biosynthesis of Species-Specific Sex Pheromones in the Genus Ostrinia (Lepidoptera: Crambidae)
Insect Sex Pheromone Research and Beyond, 2020Co-Authors: Yukio Ishikawa, Takeshi FujiiAbstract:The genus Ostrinia (Lepidoptera; Crambidae) consists of 21 species worldwide, 9 of which inhabit Japan. These closely related species, which utilize different host plants, provide an excellent model for studies on the evolution of sex pheromone communication systems as well as speciation. In this chapter, we review the progress of research on the sex pheromone communication system of Ostrinia inhabiting Japan. We focus on the molecular bases for the biosynthesis of sex pheromone components, differential production of which is responsible for the divergence of sex pheromones.
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Multiple Δ11-desaturase genes selectively used for sex pheromone biosynthesis are conserved in Ostrinia moth genomes.
Insect biochemistry and molecular biology, 2015Co-Authors: Takeshi Fujii, Yuji Yasukochi, Yu Rong, Takashi Matsuo, Yukio IshikawaAbstract:Abstract Regulation of the expression of fatty acyl-CoA desaturases, which introduce a double bond into the fatty acid moiety of the substrate, is crucial for the production of species-specific sex pheromones in moths. In Ostrinia moths, two distinct Δ11-desaturases and a Δ14-desaturase are known to be selectively used in the biosynthesis of sex pheromones. Of the two Δ11-desaturases, one identified from Ostrinia nubilalis and Ostrinia scapulalis, Z/EΔ11, forms the Z and E isomers of a double bond at position 11, whereas the other identified from Ostrinia latipennis, LATPG1(=EΔ11), exclusively forms an E double bond at position 11. Since the retroposon(ezi)-fused, non-functional Δ11-desaturase gene, ezi-Δ11α, in the genomes of O. nubilalis and O. furnacalis was previously suggested to be an orthologue of latpg1, we here explored Z/EΔ11 orthologues in the genome of O. latipennis. We newly identified two Δ11-desaturase genes, latpg2 and latpg3, which were orthologous to ezi-Δ11β and Z/EΔ11, respectively. We found that an ezi-like element was integrated in intron 1 of latpg1, and confirmed that only latpg1 was expressed in the pheromone gland of O. latipennis. Thus, at least three Δ11-desaturase genes are present in the genome of O. latipennis, and latpg1 is selectively transcribed in the pheromone gland of this moth. The non-functionality of ezi-inserted desaturase genes in O. nubilalis and O. furnacalis may not be a direct consequence of the insertion of an ezi- or ezi-like element into the gene.
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Sex pheromone desaturase functioning in a primitive Ostrinia moth is cryptically conserved in congeners' genomes.
Proceedings of the National Academy of Sciences of the United States of America, 2011Co-Authors: Takeshi Fujii, Katsuhiko Ito, Mitsuko Tatematsu, Toru Shimada, Susumu Katsuma, Yukio IshikawaAbstract:(E)-11- and (Z)-11-tetradecenyl acetate are the most common female sex pheromone components in Ostrinia moths. The Δ11-desaturase expressed in the pheromone gland (PG) of female moths is a key enzyme that introduces a double bond into pheromone molecules. A single Δ11-desaturase of Ostrinia nubilalis, OnubZ/E11, has been shown to produce an ∼7:3 mixture of (E)-11- and (Z)-11-tetradecenoate from the substrate tetradecanoate. In contrast, the sex pheromone of Ostrinia latipennis, a primitive species of Ostrinia, is (E)-11-tetradecenol. This pheromone is unique in that it is not acetylated, and includes no Z isomer. In the present study, through the cloning and functional analysis of a PG-specific Δ11-desaturase in O. latipennis, we showed that the absence of the Z isomer in the pheromone is attributable to the strict product specificity of the Δ11-desaturase in this species, LATPG1. Phylogenetic analysis revealed that LATPG1 was not closely related to OnubZ/E11. Rather, it was closely related to retroposon-linked cryptic Δ11-desaturases (ezi-Δ11) found in the genomes of O. nubilalis and Ostrinia furnacalis. Taken together, the results showed that an unusual Δ11-desaturase is functionally expressed in O. latipennis, although the genes encoding this enzyme appear to be cryptic in congeners.
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Genetic Basis Regulating the Sex Pheromone Blend in Ostrinia zealis (Lepidoptera: Crambidae) and Its Allies Inferred from Crossing Experiments
Annals of the Entomological Society of America, 2011Co-Authors: Jun Tabata, Yukio IshikawaAbstract:To gain insight into the genetic basis of the divergence of sex pheromone signals in the genus Ostrinia, we conducted crossing experiments between three congeners: Ostrinia zealis (Guenee), Ostrinia zaguliaevi Mutuura & Munroe, and Ostrinia sp. near zaguliaevi. Female sex pheromones of the three species are comprised of three common components—(Z)-9-tetradecenyl acetate, (E)-11-tetradecenyl acetate, and (Z)-11-tetradecenyl acetate—blended at species-specific ratios: 70:24:6 in O. zealis, 45:5:50 in O. zaguliaevi, and 19:6:75 in O. sp. near zaguliaevi. The differences between O. zealis and O. zaguliaevi blends, and O. zealis and O. sp. near zaguliaevi blends, could be explained by a single autosomal locus with three alleles. However, sex chromosomal as well as autosomal genes should be taken into consideration to explain the difference between O. zaguliaevi and O. sp. near zaguliaevi blends. From analyses of the fatty acyl precursors of pheromone components, it seems that these genetic factors are involved in the last steps of pheromone biosynthesis including reduction. The process of divergence of pheromone components in the genus Ostrinia is discussed with reference to the findings of previous studies.
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Physiological adaptation of the Asian corn borer Ostrinia furnacalis to chemical defenses of its host plant, maize.
Journal of insect physiology, 2010Co-Authors: Wataru Kojima, Takeshi Fujii, Momoko Suwa, Mitsuo Miyazawa, Yukio IshikawaAbstract:Abstract A number of gramineous plants such as maize contain cyclic hydroxamic acids (cHx) that are toxic to many herbivores. Among the Ostrinia species found in Japan, the Asian corn borer Ostrinia furnacalis is the only one that utilizes maize, a gramineous plant. We used O. furnacalis and two congeners, Ostrinia scapulalis and Ostrinia latipennis, to obtain insights into the physiological adaptation of O. furnacalis to cHx. When an artificial diet containing a low concentration (0.3 mg/g diet) of cHx was fed to the larvae of O. furnacalis and O. scapulalis, larval growth and survival were significantly less affected in O. furnacalis than O. scapulalis. An artificial diet containing a high level (0.7 mg/g diet) of cHx was found to severely retard the growth of both species, albeit to different degrees. In an assay in vitro, homogenate of the digestive tract of O. furnacalis larvae degraded cHx more rapidly than that of O. scapulalis or O. latipennis. The degradation was found to be enzymatic and dependent on a cofactor, UDP-glucose, suggesting that UDP-glucosyltransferase or other UDP-glucose-dependent enzymes were involved. This enzymatic adaptation probably has enabled O. furnacalis to utilize plants containing cHx.
Denis Bourguet - One of the best experts on this subject based on the ideXlab platform.
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De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Philippe Audiot, Denis Bourguet, Emmanuelle Beyne, Réjane StreiffAbstract:BackgroundThis study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics.ResultsBased on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes.ConclusionsThis data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes.
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De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis.
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Philippe Audiot, Denis Bourguet, Emmanuelle Beyne, Réjane StreiffAbstract:Background: This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics. Results: Based on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes. Conclusions: This data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes. Keywords: NGS, 454 sequencing, Tanscriptome, Moth, European corn borer, Adzuki bean borer, Sibling species, Ostrinia nubilalis, Ostrinia scapulalis
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Phylogenetics and population genetics of the Eurasian parasitoid Macrocentrus cingulum based on mitochondrial and nuclear loci
Entomologia Experimentalis et Applicata, 2012Co-Authors: Benjamin Pelissie, Denis Bourguet, Sergine Ponsard, Gael KergoatAbstract:Specifying species boundaries is often tricky, because advanced biomolecular analyses can reveal that morphologically similar individuals in fact belong to distinct species. This is frequently the case when populations previously considered as a single polyphagous taxon prove to consist of several genetically distinct taxa using different resources, e.g., among insect parasitoids. Macrocentrus cingulum Brischke (Hymenoptera: Braconidae), a parasitoid of the genus Ostrinia (Lepidoptera: Crambidae) feeding on various host plants across the world, is one of them. In Western Europe, M. cingulum has never been found in Ostrinia nubilalis (Hubner) populations feeding on maize, although it heavily parasitizes sympatric Ostrinia scapulalis Walker populations feeding on mugwort. In contrast, it contributes to pest control of Ostrinia furnacalis Guenee feeding on maize in Asia and O. nubilalis feeding on maize in America, suggesting that European and Asian M. cingulum populations might form two distinct taxa. We tested this hypothesis by conducting phylogenetic and population genetic analyses based on two mitochondrial and two nuclear genes, on 97 M. cingulum individuals sampled in Asia, USA, and Europe. Our analyses not only suggest that all sampled M. cingulum probably belong to the same species, but also show a significant genetic differentiation between individuals originating from Europe on the one hand and Asia/USA on the other, which correlates with infestation patterns. Moreover, they show that American specimens are closely related to Asian ones, consistent with historical records about M. cingulum introductions into the USA in the 1920s and 1930s to control expanding O. nubilalis populations. Combining these results with what is known about the evolutionary history within the genus Ostrinia, we offer a candidate evolutionary scenario that is amenable to future empirical testing.
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Divergence in behaviour between the European corn borer, Ostrinia nubilalis, and its sibling species Ostrinia scapulalis: adaptation to human harvesting?
Proceedings of the Royal Society B: Biological Sciences, 2010Co-Authors: Vincent Calcagno, Vincent Bonhomme, Yan Thomas, Michael Singer, Denis BourguetAbstract:Divergent adaptation to host plant species may be the major mechanism driving speciation and adaptive radiations in phytophagous insects. Host plants can differ intrinsically in a number of attributes, but the role of natural enemies in host plant specialization is often underappreciated. Here, we report behavioural divergence between the European corn borer (ECB, Ostrinia nubilalis) and its sibling species Ostrinia scapulalis, in relation to a major enemy: humans. Harvesting maize imposes selective mortality on Ostrinia larvae: those located above the cut-off line of the stalk face almost certain death. We show that ECB larvae diapause closer to the ground than those of O. scapulalis, which is sympatric but feeds mainly on weeds. The difference in diapause height results from genetically determined differences in geotactic behaviour. ECB larvae descend towards the ground specifically at harvest time, increasing their chances of surviving harvesting by about 50 per cent over O. scapulalis larvae. Natural enemies appear as a major driver of host-plant specialization in this example, stressing the need to consider ‘tri-trophic’ ecological niches to understand insect diversification. Our results also strongly suggest that geotaxis evolved as a singular instance of behavioural resistance in a major agricultural pest.
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Reconsidering the taxomony of several Ostrinia species in the light of reproductive isolation: a tale for Ernst Mayr
Biological Journal of the Linnean Society, 2007Co-Authors: Andrei N. Frolov, Denis Bourguet, Sergine PonsardAbstract:We reconsider the taxonomy of a group of closely related Ostrinia spp., illustrating how useful Mayr's biological spe- cies concept remains for studying speciation patterns and processes. We review and re-analyse recent data on Ostrinia scapulalis , Ostrinia nubilalis , Ostrinia narynensis and Ostrinia orientalis , along with those obtained over > 45 years in the former Soviet Union. The ten species of the 'trilobed uncus' group in the Ostrinia genus are clas- sified into subgroups according to male mid-tibia morphology. However, none of the characters that further discrim- inate between them (female sex pheromones, male genitalia and calling time) varies together with male mid-tibia morphology, and neither do molecular markers. Moreover, male mid-tibia morphology appears to depend on only two diallelic loci and seems to be unrelated to reproductive isolation between Ostrinia taxa. By contrast, reproductive iso- lation is strongly related to host-plant type. In accordance with Mayr's species concept, we thus propose a revision of the trilobed uncus Ostrinia spp. based primarily on host-plant type. We propose that O. narynensis Mutuura & Munroe, 1970 ( syn. nov. ) and O. orientalis Mutuura & Munroe, 1970 ( syn. nov. ) be synonymized with O. scapulalis (Walker, 1859). We further demonstrate that O. nubilalis auctt. pro parte feeding on mugwort, hop, and several other dicotyledons (previously called the ' O. nubilalis mugwort-race' in France) also belongs to O. scapulalis . Consequently, we propose that only O. nubilalis specimens feeding on maize (the former French ' O. nubilalis maize- race') belong to O. nubilalis (Hubner, 1796). The implications of this revision are discussed. © 2007 The Linnean Society of London, Biological Journal of the Linnean Society , 2007, 91 , 49-72. ADDITIONAL KEYWORDS: biological species concept - host-races - Ostrinia furnacalis - Ostrinia narynensis - Ostrinia nubilalis - Ostrinia orientalis - Ostrinia scapulalis - speciation - taxonomy.
Réjane Streiff - One of the best experts on this subject based on the ideXlab platform.
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Complete mitogenome data from a European specimen of Ostrinia scapulalis (Walker, 1859) (Lepidoptera, Pyraloidea, Crambidae, Pyraustinae).
Data in brief, 2020Co-Authors: Bernhard Gschloessl, Philippe Audiot, Sabine Nidelet, Gael J. Kergoat, Réjane StreiffAbstract:We present an assembly and annotation of the mitogenome of a European specimen of the Adzuki bean borer, Ostrinia scapulalis (Walker, 1859). The data were obtained by combining WGS data issue of a de novo and a previously published sequence library (Gschloessl et al., 2018). We also provide the phylogenetic positioning of the mitogenome within the Ostrinia genus, the Crambidae family and with more distant Lepidoptera species.
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De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Philippe Audiot, Denis Bourguet, Emmanuelle Beyne, Réjane StreiffAbstract:BackgroundThis study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics.ResultsBased on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes.ConclusionsThis data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes.
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De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis.
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Philippe Audiot, Denis Bourguet, Emmanuelle Beyne, Réjane StreiffAbstract:Background: This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics. Results: Based on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes. Conclusions: This data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes. Keywords: NGS, 454 sequencing, Tanscriptome, Moth, European corn borer, Adzuki bean borer, Sibling species, Ostrinia nubilalis, Ostrinia scapulalis
Blair D Siegfried - One of the best experts on this subject based on the ideXlab platform.
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comparative binding of cry1ab and cry1f bacillus thuringiensis toxins to brush border membrane proteins from Ostrinia nubilalis Ostrinia furnacalis and diatraea saccharalis lepidoptera crambidae midgut tissue
Journal of Invertebrate Pathology, 2013Co-Authors: B F Cayabyab, Edwin P Alcantara, Fangneng Huang, Kanglai He, Kenneth W Nickerson, Blair D SiegfriedAbstract:Abstract The European (Ostrinia nubilalis Hubner) and Asian corn borers (Ostrinia furnacalis Guenee) are closely related and display similar sensitivity to Cry1 toxins. In this study, we compared the binding patterns of Cry1Ab and Cry1F toxins between both Ostrinia spp., as well as the expression of putative cadherin- and aminopeptidase-N (APN)-like protein receptors. Additionally, cDNA sequences of these putative toxin receptors from both Ostrinia species were compared. Ligand blots for both species indicated a similar binding pattern for Cry1Ab with the strongest immunoreactive band at 260 kDa in both species. In addition, similar expression of the putative cadherin- and APN-like protein receptors were observed at 260 and 135 kDa, respectively. A high degree of similarity (98% amino acid sequence identity) of cDNA sequences for both putative receptor sequences was observed. The Cry1F ligand blot revealed that O. furnacalis and O. nubilalis BBMV exhibited slightly different binding patterns, with strong binding to putative proteins at 150 and 140 kDa, respectively. Both proteins appeared to also bind Cry1Ab, although the signal intensity was much reduced with Cry1Ab. O. furnacalis showed an additional but weaker band at 210 kDa relative to the 150 kDa band. Diatraea saccharalis (Fabricius), which was used as an outgroup species, exhibited different binding patterns than either Ostrinia species, with both Cry1Ab and Cry1F toxins binding to a 210 kDa protein. These results support the previous experiments indicating that O. nubilalis and O. furnacalis share similar patterns of susceptibility to Cry toxins.
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comparative binding of cry1ab and cry1f bacillus thuringiensis toxins to brush border membrane proteins from Ostrinia nubilalis Ostrinia furnacalis and diatraea saccharalis lepidoptera crambidae midgut tissue
Journal of Invertebrate Pathology, 2013Co-Authors: Sek Yee Tan, B F Cayabyab, Edwin P Alcantara, Fangneng Huang, Kenneth W Nickerson, Blair D SiegfriedAbstract:The European (Ostrinia nubilalis Hubner) and Asian corn borers (Ostrinia furnacalis Guenee) are closely related and display similar sensitivity to Cry1 toxins. In this study, we compared the binding patterns of Cry1Ab and Cry1F toxins between both Ostrinia spp., as well as the expression of putative cadherin- and aminopeptidase-N (APN)-like protein receptors. Additionally, cDNA sequences of these putative toxin receptors from both Ostrinia species were compared. Ligand blots for both species indicated a similar binding pattern for Cry1Ab with the strongest immunoreactive band at 260 kDa in both species. In addition, similar expression of the putative cadherin- and APN-like protein receptors were observed at 260 and 135 kDa, respectively. A high degree of similarity (98% amino acid sequence identity) of cDNA sequences for both putative receptor sequences was observed. The Cry1F ligand blot revealed that O. furnacalis and O. nubilalis BBMV exhibited slightly different binding patterns, with strong binding to putative proteins at 150 and 140 kDa, respectively. Both proteins appeared to also bind Cry1Ab, although the signal intensity was much reduced with Cry1Ab. O. furnacalis showed an additional but weaker band at 210 kDa relative to the 150 kDa band. Diatraea saccharalis (Fabricius), which was used as an outgroup species, exhibited different binding patterns than either Ostrinia species, with both Cry1Ab and Cry1F toxins binding to a 210 kDa protein. These results support the previous experiments indicating that O. nubilalis and O. furnacalis share similar patterns of susceptibility to Cry toxins.
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comparative susceptibility of Ostrinia furnacalis Ostrinia nubilalis and diatraea saccharalis lepidoptera crambidae to bacillus thuringiensis cry1 toxins
Crop Protection, 2011Co-Authors: B F Cayabyab, Edwin P Alcantara, Fangneng Huang, Yusof Ibrahim, Erin E Blankenship, Blair D SiegfriedAbstract:Transgenic corn hybrids that express toxins from Bacillus thuringiensis (Bt) are highly effective against the European corn borer, Ostrinia nubilalis (Hubner), and the closely related Asian corn borer, Ostrinia furnacalis (Guenee). Since the registration of Bt corn hybrids in the U.S. in 1996, there has been a great deal of information generated on O. nubilalis. However, relatively little information exists for O. furnacalis. To help determine whether the information generated for O. nubilalis can be leveraged for decisions regarding the use of transgenic Bt corn against O. furnacalis, experiments were designed to determine whether the pattern of sensitivity to various Bt Cry1 toxins is similar between the two species. Test insects included laboratory-reared O. furnacalis originating from Malaysia, a Bt-susceptible laboratory colony of O. nubilalis maintained at the University of Nebraska-Lincoln (UNL) and an out-group consisting of the sugarcane borer, Diatraea saccharalis (F.), from Louisiana which represents a different genus from the same family. O. furnacalis and O. nubilalis exhibited a similar pattern of susceptibility to all the Cry1 toxins and were highly susceptible to the range of Bt toxins tested including Cry1Aa, Cry1Ab, Cry1Ac and Cry1F. Both of the Ostrinia species were more tolerant to Cry1Ba compared with D. saccharalis, although sensitivity of O. furnacalis was intermediate and did not differ significantly from that of O. nubilalis and D. saccharalis. D. saccharalis was also susceptible to the range of toxins tested but unlike the two Ostrinia species, was more tolerant to Cry1F and more susceptible to Cry1Ba. These results indicate that both of the Ostrinia corn borer species are similar in sensitivity to the Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ba and Cry1F toxins, thus suggesting shared toxin receptors and mechanisms of toxicity for the two species.
Philippe Audiot - One of the best experts on this subject based on the ideXlab platform.
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Complete mitogenome data from a European specimen of Ostrinia scapulalis (Walker, 1859) (Lepidoptera, Pyraloidea, Crambidae, Pyraustinae).
Data in brief, 2020Co-Authors: Bernhard Gschloessl, Philippe Audiot, Sabine Nidelet, Gael J. Kergoat, Réjane StreiffAbstract:We present an assembly and annotation of the mitogenome of a European specimen of the Adzuki bean borer, Ostrinia scapulalis (Walker, 1859). The data were obtained by combining WGS data issue of a de novo and a previously published sequence library (Gschloessl et al., 2018). We also provide the phylogenetic positioning of the mitogenome within the Ostrinia genus, the Crambidae family and with more distant Lepidoptera species.
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De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Philippe Audiot, Denis Bourguet, Emmanuelle Beyne, Réjane StreiffAbstract:BackgroundThis study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics.ResultsBased on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes.ConclusionsThis data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes.
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De novo transcriptomic resources for two sibling species of moths: Ostrinia nubilalis and O. scapulalis.
BMC Research Notes, 2013Co-Authors: Bernhard Gschloessl, Philippe Audiot, Denis Bourguet, Emmanuelle Beyne, Réjane StreiffAbstract:Background: This study aimed at enhancing the transcriptomic resources for two sibling species of moths, Ostrinia scapulalis (Adzuki bean borer) and Ostrinia nubilalis (European corn borer), as a foundation for future researches on their divergence history. Previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. Comparative genomic resources will help facilitate the understanding of the mechanisms involved in this isolation and adaptation to the host plants. Despite their fundamental interest, these species still lack the genomic resources to thoroughly identify candidate genes for functions of interest. We present here a high throughput sequencing and de novo transcriptome assembly for these two sibling species in line with this objective of comparative genomics. Results: Based on 322,504 and 307,622 reads of 454 sequencing for O. scapulalis and O. nubilalis respectively, we reconstructed 11,231 and 10,773 transcripts, of which 40% were functionally annotated by BLAST analyzes. We determined the level of completeness of both assemblies as well as the recovery level of published Ostrinia genomic resources. Gene ontology (GO) of common and species-specific de novo transcripts did not reveal GO terms significantly enriched in one or the other species. By applying stringent homology searches on transcripts common to O. scapulalis and O. nubilalis, we identified a set of homologous transcripts, with a mean nucleotide identity value of 98.1%. In this set, the most divergent transcripts revealed candidate genes involved in developmental, sensorial and pathogen defense processes. Conclusions: This data greatly increases the genomic resources of Ostrinia species and constitute a solid skeleton for future comparative analyzes of expression or diversity, despite we show that the transcriptomes for both species have not been assembled at full completion. In addition, we provide a set of homologous transcripts together with their annotation as a source of candidate genes for comparative analyzes. Keywords: NGS, 454 sequencing, Tanscriptome, Moth, European corn borer, Adzuki bean borer, Sibling species, Ostrinia nubilalis, Ostrinia scapulalis
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did the introduction of maize into europe provide enemy free space to Ostrinia nubilalis parasitism differences between two sibling species of the genus Ostrinia
Journal of Evolutionary Biology, 2010Co-Authors: Philippe Audiot, Sergine Ponsard, Benjamin Pelissie, Yuri S Tokarev, Celine Pelissier, Rodolphe Sabatier, Serge MeusnierAbstract:We examined whether maize offers enemy-free space (EFS) to its pest Ostrinia nubilalis, and may thereby have contributed to its divergence from the sibling species, Ostrinia scapulalis, feeding mainly on mugwort, when introduced into Europe five centuries ago. We collected Ostrinia larvae on maize (70 populations, 8425 individuals) and mugwort (10 populations, 1184 individuals) and recorded parasitism using both traditional (counting emerging parasitoids) and molecular methods (detection by specific polymerase chain reaction). The main parasitoid was Macrocentrus cingulum (Braconidae). On mugwort, parasitism was twice that on maize, and parasitoid-related mortality was 8 times higher. This suggests that maize affords substantial EFS to Ostrinia feeding on it. The lower Mortality:Infestation ratio in maize suggests that O. nubilalis' immune response might be stronger than that of O. scapulalis. If so, adapting to maize and diverging from O. scapulalis would decrease the impact of parasitism on O. nubilalis at both ecological and evolutionary levels.
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Did the introduction of maize into Europe provide enemy‐free space to Ostrinia nubilalis? Parasitism differences between two sibling species of the genus Ostrinia
Journal of evolutionary biology, 2009Co-Authors: Benjamin Pelissie, Philippe Audiot, Sergine Ponsard, Yuri S Tokarev, Celine Pelissier, Rodolphe Sabatier, Serge Meusnier, Josette Chaufaux, Marc Delos, Eric CampanAbstract:We examined whether maize offers enemy-free space (EFS) to its pest Ostrinia nubilalis, and may thereby have contributed to its divergence from the sibling species, Ostrinia scapulalis, feeding mainly on mugwort, when introduced into Europe five centuries ago. We collected Ostrinia larvae on maize (70 populations, 8425 individuals) and mugwort (10 populations, 1184 individuals) and recorded parasitism using both traditional (counting emerging parasitoids) and molecular methods (detection by specific polymerase chain reaction). The main parasitoid was Macrocentrus cingulum (Braconidae). On mugwort, parasitism was twice that on maize, and parasitoid-related mortality was 8 times higher. This suggests that maize affords substantial EFS to Ostrinia feeding on it. The lower Mortality:Infestation ratio in maize suggests that O. nubilalis' immune response might be stronger than that of O. scapulalis. If so, adapting to maize and diverging from O. scapulalis would decrease the impact of parasitism on O. nubilalis at both ecological and evolutionary levels.
