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Teresa Szklarzewicz - One of the best experts on this subject based on the ideXlab platform.
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Symbiotic microorganisms in Puto superbus (Leonardi, 1907) (Insecta, Hemiptera, Coccomorpha: Putoidae).
Protoplasma, 2017Co-Authors: Teresa Szklarzewicz, Władysława Jankowska, Małgorzata Kalandyk-kołodziejczyk, Katarzyna Michalik, Anna MichalikAbstract:The scale insect Puto superbus (Putoidae) lives in mutualistic symbiotic association with bacteria. Molecular phylogenetic analyses have revealed that symbionts of P. superbus belong to the gammaproteobacterial genus Sodalis. In the adult females, symbionts occur both in the bacteriocytes constituting compact bacteriomes and in individual bacteriocytes, which are dispersed among Ovarioles. The bacteriocytes also house a few small, rod-shaped Wolbachia bacteria in addition to the numerous large, elongated Sodalis-allied bacteria. The symbiotic microorganisms are transovarially transmitted from generation to generation. In adult females which have choriogenic oocytes in the Ovarioles, the bacteriocytes gather around the basal part of the tropharium. Next, the entire bacteriocytes pass through the follicular epithelium surrounding the neck region of the Ovariole and enter the space between oocyte and follicular epithelium (perivitelline space). In the perivitelline space, the bacteriocytes assemble extracellularly in the deep depression of the oolemma at the anterior pole of the oocyte, forming a “symbiont ball”.
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Ovary of Matsucoccus pini (Insecta, Hemiptera, Coccinea: Matsucoccidae): morphology, ultrastructure, and phylogenetic implications.
Microscopy research and technique, 2014Co-Authors: Teresa Szklarzewicz, Anna Michalik, Małgorzata Kalandyk-kołodziejczyk, Michal Kobialka, Ewa SimonAbstract:The structure of ovary in a representative of the scale insect family Matsucoccidae, Matsucoccus pini, is described at the ultrastructural level. The paired ovaries of M. pini are composed of about 50 Ovarioles of telotrophic type that develop asynchronously. An individual Ovariole consists of an anterior tropharium (trophic chamber) and posterior vitellarium. The tropharium encloses trophocytes (nurse cells) and early previtellogenic oocytes termed arrested oocytes. In the vitellarium from 1 to 6, linearly arranged oocytes may develop. Analysis of serial sections has shown that each Ovariole contains 32 germ cells (trophocytes, arrested oocytes, and developing oocytes). In the cytoplasm of all these cells, small rod-shaped bacteria are present. In the early vitellogenic oocytes, accessory nuclei arise. As vitellogenesis progresses, these nuclei migrate toward the cortical ooplasm. The obtained results are discussed in a phylogenetic context. Microsc. Res. Tech. 77:327–334, 2014. © 2014 Wiley Periodicals, Inc.
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the ovaries of aphids hemiptera sternorrhyncha aphidoidea morphology and phylogenetic implications
Invertebrate Biology, 2013Co-Authors: Anna Michalik, Teresa Szklarzewicz, Piotr Wegierek, Karina WieczorekAbstract:The ovaries of aphids belonging to the families Eriosomatidae, Anoeciidae, Drepanosiphidae, Thelaxidae, Aphididae, and Lachnidae were examined at the ultrastructural level. The ovaries of these aphids are composed of several telotrophic Ovarioles. The individual Ovariole is differentiated into a terminal filament, tropharium, vitellarium, and pedicel (ovariolar stalk). Terminal filaments of all Ovarioles join together into the suspensory ligament, which attaches the ovary to the lobe of the fat body. The tropharium houses individual trophocytes and early previtellogenic oocytes termed arrested oocytes. Trophocytes are connected with the central part of the tropharium, the trophic core, by means of broad cytoplasmic processes. One or more oocytes develop in the vitellarium. Oocytes are surrounded by a single layer of follicular cells, which do not diversify into distinct subpopulations. The general organization of the ovaries in oviparous females is similar to that of the ovaries in viviparous females, but there are significant differences in their functioning: (1) in viviparous females, all Ovarioles develop, whereas in oviparous females, some of them degenerate; (2) the number of germ cells per Ovariole is usually greater in females of the oviparous generation than in females of viviparous generations; (3) in oviparous females, oocytes in the vitellarium develop through three stages (previtellogenesis, vitellogenesis, and choriogenesis), whereas in viviparous females, the development of oocytes stops after previtellogenesis; and (4) in the oocyte cytoplasm of oviparous females, lipid droplets and yolk granules accumulate, whereas in viviparous females, oocytes accrue only lipid droplets. Our results indicate that a large number of germ cells per Ovariole represent the ancestral state within aphids. This trait may be helpful in inferring the phylogeny of Aphidoidea.
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Ovaries of Puto superbus and Ceroputo pilosellae (Hemiptera: Coccoidea): Morphology, ultrastructure, phylogenetic and taxonomic implications
European Journal of Entomology, 2013Co-Authors: Anna Michalik, Małgorzata Kalandyk-kołodziejczyk, Ewa Simon, Michał Kobiałka, Teresa SzklarzewiczAbstract:Ovaries of Puto superbus and Ceroputo pilosellae are composed of numerous short telotrophic Ovarioles that are arranged around the distal part of the lateral oviduct. An individual Ovariole consists of a distal trophic chamber (= tropharium) and proximal vitellarium. The tropharia enclose individual trophocytes (= nurse cells) and early previtellogenic oocytes termed arrested oocytes. A single oocyte develops in each vitellarium. Analysis of serial sections has shown that Ovarioles of P. superbus contain from 16 to 51 germ cells (13-43 trophocytes, 2-7 arrested oocytes, 1 developing oocyte) while those of C. pilosellae from only 8 to 10 germ cells (5-7 trophocytes, 0-2 arrested oocytes, 1 developing oocyte). The classification and phylogeny of scale insects are discussed taking into consideration the results of this study.
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Germ cell cluster formation and Ovariole structure in Puto albicans and Crypticerya morrilli (Hemiptera: Coccinea). Phylogenetic implications
European Journal of Entomology, 2010Co-Authors: Teresa Szklarzewicz, Anna Michalik, Anna Czaja, Sylwia SzydlowskaAbstract:The organization and development of ovaries in representatives of two families (Putoidae and Monophlebidae) of scale insects are described. Developing ovaries of Puto albicans McKenzie, 1967 and Crypticerya morrilli (Cockerell, 1914) consist of numerous clusters of cystocytes that are arranged in the form of rosettes. At the end of the last nymphal instar these clusters start to protrude from the interior of the ovary into the body cavity and the Ovarioles begin to be formed. The ovary of a young female is composed of about 200 spherical telotrophic Ovarioles devoid of terminal filaments. The Ovarioles of C. morrilli contain 8 germ cells (7 trophocytes and a single oocyte). From 25 to 45 germ cells (23-43 trophocytes and 2 or 3 oocytes) occur in the Ovarioles of P. albicans. An Ovariole of an adult female is subdivided into a trophic chamber (tropharium), vitellarium and ovariolar stalk (pedicel). At each stage of development, the ovaries are accompanied by large cells (termed bacteriocytes) that contain endosymbiotic microorganisms. The organization of the ovary in P. albicans is more similar to that in archaeococcoid scale insects than in neococcoid taxa. In contrast, the number of germ cells per Ovariole in C. morrilli is not typical of other archaeococcoids, but resembles the derived condition seen in other iceryine taxa. The classification and phylogeny of scale insects are discussed in the light of these results.
Márcia Maria Gentile Bitondi - One of the best experts on this subject based on the ideXlab platform.
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Ovariole structure and stages characterization.
2016Co-Authors: Juliana Ramos Martins, Márcia Maria Gentile BitondiAbstract:The Ovariole comprises the anterior terminal filament, germarium and developing follicles (vitellarium) as indicated at the right. The oocytes are marked in yellow. The Ovarioles are categorized according to the degree of development in stages S0, S1, S2, S3 and S4. Stage S0: presence of initial oocytes (cystocytes) and precursors of nurse and somatic cells in the germarium. Stage S1: an oocyte accompanied by differentiated nurse cells is positioned at the basal region of the Ovariole. Stage S2: a slight constriction between the basal oocyte and accompanying nurse cells marks the beginning of oocyte chamber and nurse cell chamber separation; the basal oocyte is surrounded by follicle cells. Stage S3: a sequence of developing oocytes and nurse cell chambers is evident in the Ovariole; the larger oocyte at the basal region is well-separated from the nurse cell chamber. Stage S4: presence of several developing oocytes and nurse cell chambers through the Ovariole; a quasi-fully or a fully developed oocyte is evident at the most basal region. The classification of the maturing oocytes in stages 1 to 7 (stage 6 is not shown) was based in Wilson et al. [44].
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Immunolocalization of HEX 110 in RNase A-treated Ovarioles (stage S2) of queenright nurse workers.
2016Co-Authors: Juliana Ramos Martins, Márcia Maria Gentile BitondiAbstract:(A) Control Ovariole non-incubated with RNase A. HEX 110 green foci (labeled with anti-HEX 110/Alexa-Fluor 488) are abundant in the nucleus (arrowheads) and cytoplasm (arrows) of nurse (Nc) and follicle (Fc) cells surrounding the oocyte (stage 2, see Fig 2). (B) Ovariole incubated with RNase A shows scarce HEX 110 foci (arrows). DAPI-staining (blue) in B highlights the small nuclei of follicle cells (Fc) that surround the oocyte (stage 1, see Fig 2) and are interspersed between the large nurse cell (Nc) nuclei. Quantification of pixels intensity in the selected areas (dashed rectangles) of the images obtained from RNase A-treated and untreated Ovarioles showed lower intensity in RNase A-treated (Mean: 17.29; Min/Max gray values: 0/255; IntDen: 575.51) than in untreated controls (Mean: 29.39; Min/Max: 0/255; IntDen: 978.45).
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A Honey Bee Hexamerin, HEX 70a, Is Likely to Play an Intranuclear Role in Developing and Mature Ovarioles and Testioles
PloS one, 2011Co-Authors: Juliana Martins, Zilá Luz Paulino Simões, Lucas Anhezini, Rodrigo Pires Dallacqua, Márcia Maria Gentile BitondiAbstract:Insect hexamerins have long been known as storage proteins that are massively synthesized by the larval fat body and secreted into hemolymph. Following the larval-to-pupal molt, hexamerins are sequestered by the fat body via receptor-mediated endocytosis, broken up, and used as amino acid resources for metamorphosis. In the honey bee, the transcript and protein subunit of a hexamerin, HEX 70a, were also detected in ovaries and testes. Aiming to identify the subcellular localization of HEX 70a in the female and male gonads, we used a specific antibody in whole mount preparations of ovaries and testes for analysis by confocal laser-scanning microscopy. Intranuclear HEX 70a foci were evidenced in germ and somatic cells of Ovarioles and testioles of pharate-adult workers and drones, suggesting a regulatory or structural role. Following injection of the thymidine analog EdU we observed co-labeling with HEX 70a in Ovariole cell nuclei, inferring possible HEX 70a involvement in cell proliferation. Further support to this hypothesis came from an injection of anti-HEX 70a into newly ecdysed queen pupae where it had a negative effect on Ovariole thickening. HEX 70a foci were also detected in Ovarioles of egg laying queens, particularly in the nuclei of the highly polyploid nurse cells and in proliferating follicle cells. Additional roles for this storage protein are indicated by the detection of nuclear HEX 70a foci in post-meiotic spermatids and spermatozoa. Taken together, these results imply undescribed roles for HEX 70a in the developing gonads of the honey bee and raise the possibility that other hexamerins may also have tissue specific functions.
Sylwia Niznik - One of the best experts on this subject based on the ideXlab platform.
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Ultrastructural Studies of the Ovary of Palaeococcus fuscipennis (Burmeister) (Insecta, Hemiptera, Coccinea: Monophlebidae)*
Folia Biologica, 2005Co-Authors: Teresa Szklarzewicz, Katarzyna Kedra, Sylwia NiznikAbstract:SZKLARZEWICZ T., KEDRA K., NINIK S. 2005. Ultrastructural studies of the ovary of Palaeococcus fuscipennis (Burmeister) (Insecta, Hemiptera, Coccinea: Monophlebidae). Folia biol. (Krakow) 53: 45-50. Ovaries of Palaeocoocus fuscipennis are composed of about 100 telotrophic Ovarioles that are devoid of terminal filaments. In the Ovariole a tropharium (=trophic chamber) and vitellarium can be distinguished. The tropharium contains 7 trophocytes. A single oocyte develops in the vitellarium. The oocyte is surrounded by follicular cells that do not undergo diversification into subpopulations. The obtained results are discussed in a phylogenetic context.
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Ultrastructural studies of the ovary of Palaeococcus fuscipennis (Burmeister) (Insecta, Hemiptera, Coccinea: Monophlebidae).
Folia biologica, 2005Co-Authors: Teresa Szklarzewicz, Katarzyna Kedra, Sylwia NiznikAbstract:Ovaries of Palaeocoocus fuscipennis are composed of about 100 telotrophic Ovarioles that are devoid of terminal filaments. In the Ovariole a tropharium ( = trophic chamber) and vitellarium can be distinguished. The tropharium contains 7 trophocytes. A single oocyte develops in the vitellarium. The oocyte is surrounded by follicular cells that do not undergo diversification into subpopulations. The obtained results are discussed in a phylogenetic context.
Juliana Martins - One of the best experts on this subject based on the ideXlab platform.
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A Honey Bee Hexamerin, HEX 70a, Is Likely to Play an Intranuclear Role in Developing and Mature Ovarioles and Testioles
PloS one, 2011Co-Authors: Juliana Martins, Zilá Luz Paulino Simões, Lucas Anhezini, Rodrigo Pires Dallacqua, Márcia Maria Gentile BitondiAbstract:Insect hexamerins have long been known as storage proteins that are massively synthesized by the larval fat body and secreted into hemolymph. Following the larval-to-pupal molt, hexamerins are sequestered by the fat body via receptor-mediated endocytosis, broken up, and used as amino acid resources for metamorphosis. In the honey bee, the transcript and protein subunit of a hexamerin, HEX 70a, were also detected in ovaries and testes. Aiming to identify the subcellular localization of HEX 70a in the female and male gonads, we used a specific antibody in whole mount preparations of ovaries and testes for analysis by confocal laser-scanning microscopy. Intranuclear HEX 70a foci were evidenced in germ and somatic cells of Ovarioles and testioles of pharate-adult workers and drones, suggesting a regulatory or structural role. Following injection of the thymidine analog EdU we observed co-labeling with HEX 70a in Ovariole cell nuclei, inferring possible HEX 70a involvement in cell proliferation. Further support to this hypothesis came from an injection of anti-HEX 70a into newly ecdysed queen pupae where it had a negative effect on Ovariole thickening. HEX 70a foci were also detected in Ovarioles of egg laying queens, particularly in the nuclei of the highly polyploid nurse cells and in proliferating follicle cells. Additional roles for this storage protein are indicated by the detection of nuclear HEX 70a foci in post-meiotic spermatids and spermatozoa. Taken together, these results imply undescribed roles for HEX 70a in the developing gonads of the honey bee and raise the possibility that other hexamerins may also have tissue specific functions.
Anna Michalik - One of the best experts on this subject based on the ideXlab platform.
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Symbiotic microorganisms in Puto superbus (Leonardi, 1907) (Insecta, Hemiptera, Coccomorpha: Putoidae).
Protoplasma, 2017Co-Authors: Teresa Szklarzewicz, Władysława Jankowska, Małgorzata Kalandyk-kołodziejczyk, Katarzyna Michalik, Anna MichalikAbstract:The scale insect Puto superbus (Putoidae) lives in mutualistic symbiotic association with bacteria. Molecular phylogenetic analyses have revealed that symbionts of P. superbus belong to the gammaproteobacterial genus Sodalis. In the adult females, symbionts occur both in the bacteriocytes constituting compact bacteriomes and in individual bacteriocytes, which are dispersed among Ovarioles. The bacteriocytes also house a few small, rod-shaped Wolbachia bacteria in addition to the numerous large, elongated Sodalis-allied bacteria. The symbiotic microorganisms are transovarially transmitted from generation to generation. In adult females which have choriogenic oocytes in the Ovarioles, the bacteriocytes gather around the basal part of the tropharium. Next, the entire bacteriocytes pass through the follicular epithelium surrounding the neck region of the Ovariole and enter the space between oocyte and follicular epithelium (perivitelline space). In the perivitelline space, the bacteriocytes assemble extracellularly in the deep depression of the oolemma at the anterior pole of the oocyte, forming a “symbiont ball”.
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Ovary of Matsucoccus pini (Insecta, Hemiptera, Coccinea: Matsucoccidae): morphology, ultrastructure, and phylogenetic implications.
Microscopy research and technique, 2014Co-Authors: Teresa Szklarzewicz, Anna Michalik, Małgorzata Kalandyk-kołodziejczyk, Michal Kobialka, Ewa SimonAbstract:The structure of ovary in a representative of the scale insect family Matsucoccidae, Matsucoccus pini, is described at the ultrastructural level. The paired ovaries of M. pini are composed of about 50 Ovarioles of telotrophic type that develop asynchronously. An individual Ovariole consists of an anterior tropharium (trophic chamber) and posterior vitellarium. The tropharium encloses trophocytes (nurse cells) and early previtellogenic oocytes termed arrested oocytes. In the vitellarium from 1 to 6, linearly arranged oocytes may develop. Analysis of serial sections has shown that each Ovariole contains 32 germ cells (trophocytes, arrested oocytes, and developing oocytes). In the cytoplasm of all these cells, small rod-shaped bacteria are present. In the early vitellogenic oocytes, accessory nuclei arise. As vitellogenesis progresses, these nuclei migrate toward the cortical ooplasm. The obtained results are discussed in a phylogenetic context. Microsc. Res. Tech. 77:327–334, 2014. © 2014 Wiley Periodicals, Inc.
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the ovaries of aphids hemiptera sternorrhyncha aphidoidea morphology and phylogenetic implications
Invertebrate Biology, 2013Co-Authors: Anna Michalik, Teresa Szklarzewicz, Piotr Wegierek, Karina WieczorekAbstract:The ovaries of aphids belonging to the families Eriosomatidae, Anoeciidae, Drepanosiphidae, Thelaxidae, Aphididae, and Lachnidae were examined at the ultrastructural level. The ovaries of these aphids are composed of several telotrophic Ovarioles. The individual Ovariole is differentiated into a terminal filament, tropharium, vitellarium, and pedicel (ovariolar stalk). Terminal filaments of all Ovarioles join together into the suspensory ligament, which attaches the ovary to the lobe of the fat body. The tropharium houses individual trophocytes and early previtellogenic oocytes termed arrested oocytes. Trophocytes are connected with the central part of the tropharium, the trophic core, by means of broad cytoplasmic processes. One or more oocytes develop in the vitellarium. Oocytes are surrounded by a single layer of follicular cells, which do not diversify into distinct subpopulations. The general organization of the ovaries in oviparous females is similar to that of the ovaries in viviparous females, but there are significant differences in their functioning: (1) in viviparous females, all Ovarioles develop, whereas in oviparous females, some of them degenerate; (2) the number of germ cells per Ovariole is usually greater in females of the oviparous generation than in females of viviparous generations; (3) in oviparous females, oocytes in the vitellarium develop through three stages (previtellogenesis, vitellogenesis, and choriogenesis), whereas in viviparous females, the development of oocytes stops after previtellogenesis; and (4) in the oocyte cytoplasm of oviparous females, lipid droplets and yolk granules accumulate, whereas in viviparous females, oocytes accrue only lipid droplets. Our results indicate that a large number of germ cells per Ovariole represent the ancestral state within aphids. This trait may be helpful in inferring the phylogeny of Aphidoidea.
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Ovaries of Puto superbus and Ceroputo pilosellae (Hemiptera: Coccoidea): Morphology, ultrastructure, phylogenetic and taxonomic implications
European Journal of Entomology, 2013Co-Authors: Anna Michalik, Małgorzata Kalandyk-kołodziejczyk, Ewa Simon, Michał Kobiałka, Teresa SzklarzewiczAbstract:Ovaries of Puto superbus and Ceroputo pilosellae are composed of numerous short telotrophic Ovarioles that are arranged around the distal part of the lateral oviduct. An individual Ovariole consists of a distal trophic chamber (= tropharium) and proximal vitellarium. The tropharia enclose individual trophocytes (= nurse cells) and early previtellogenic oocytes termed arrested oocytes. A single oocyte develops in each vitellarium. Analysis of serial sections has shown that Ovarioles of P. superbus contain from 16 to 51 germ cells (13-43 trophocytes, 2-7 arrested oocytes, 1 developing oocyte) while those of C. pilosellae from only 8 to 10 germ cells (5-7 trophocytes, 0-2 arrested oocytes, 1 developing oocyte). The classification and phylogeny of scale insects are discussed taking into consideration the results of this study.
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Germ cell cluster formation and Ovariole structure in Puto albicans and Crypticerya morrilli (Hemiptera: Coccinea). Phylogenetic implications
European Journal of Entomology, 2010Co-Authors: Teresa Szklarzewicz, Anna Michalik, Anna Czaja, Sylwia SzydlowskaAbstract:The organization and development of ovaries in representatives of two families (Putoidae and Monophlebidae) of scale insects are described. Developing ovaries of Puto albicans McKenzie, 1967 and Crypticerya morrilli (Cockerell, 1914) consist of numerous clusters of cystocytes that are arranged in the form of rosettes. At the end of the last nymphal instar these clusters start to protrude from the interior of the ovary into the body cavity and the Ovarioles begin to be formed. The ovary of a young female is composed of about 200 spherical telotrophic Ovarioles devoid of terminal filaments. The Ovarioles of C. morrilli contain 8 germ cells (7 trophocytes and a single oocyte). From 25 to 45 germ cells (23-43 trophocytes and 2 or 3 oocytes) occur in the Ovarioles of P. albicans. An Ovariole of an adult female is subdivided into a trophic chamber (tropharium), vitellarium and ovariolar stalk (pedicel). At each stage of development, the ovaries are accompanied by large cells (termed bacteriocytes) that contain endosymbiotic microorganisms. The organization of the ovary in P. albicans is more similar to that in archaeococcoid scale insects than in neococcoid taxa. In contrast, the number of germ cells per Ovariole in C. morrilli is not typical of other archaeococcoids, but resembles the derived condition seen in other iceryine taxa. The classification and phylogeny of scale insects are discussed in the light of these results.