The Experts below are selected from a list of 3 Experts worldwide ranked by ideXlab platform
Hong Lin Liu - One of the best experts on this subject based on the ideXlab platform.
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expression and preliminary functional profiling of the let 7 family during porcine Ovary Follicle Atresia
Molecules and Cells, 2015Co-Authors: Rui Cao, Xiaolong Zhou, Peng Xiao, Yi Wang, Hong Lin LiuAbstract:Most Follicles in the mammalian Ovary undergo Atresia. Granulosa cell apoptosis is a hallmark of Follicle Atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular Atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) Ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in Follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine Ovary Follicles compared with healthy Follicles, while let-7g was highly expressed during Follicle Atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.
Rui Cao - One of the best experts on this subject based on the ideXlab platform.
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expression and preliminary functional profiling of the let 7 family during porcine Ovary Follicle Atresia
Molecules and Cells, 2015Co-Authors: Rui Cao, Xiaolong Zhou, Peng Xiao, Yi Wang, Hong Lin LiuAbstract:Most Follicles in the mammalian Ovary undergo Atresia. Granulosa cell apoptosis is a hallmark of Follicle Atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular Atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) Ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in Follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine Ovary Follicles compared with healthy Follicles, while let-7g was highly expressed during Follicle Atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.
Xiaolong Zhou - One of the best experts on this subject based on the ideXlab platform.
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expression and preliminary functional profiling of the let 7 family during porcine Ovary Follicle Atresia
Molecules and Cells, 2015Co-Authors: Rui Cao, Xiaolong Zhou, Peng Xiao, Yi Wang, Hong Lin LiuAbstract:Most Follicles in the mammalian Ovary undergo Atresia. Granulosa cell apoptosis is a hallmark of Follicle Atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular Atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) Ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in Follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine Ovary Follicles compared with healthy Follicles, while let-7g was highly expressed during Follicle Atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.
Peng Xiao - One of the best experts on this subject based on the ideXlab platform.
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expression and preliminary functional profiling of the let 7 family during porcine Ovary Follicle Atresia
Molecules and Cells, 2015Co-Authors: Rui Cao, Xiaolong Zhou, Peng Xiao, Yi Wang, Hong Lin LiuAbstract:Most Follicles in the mammalian Ovary undergo Atresia. Granulosa cell apoptosis is a hallmark of Follicle Atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular Atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) Ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in Follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine Ovary Follicles compared with healthy Follicles, while let-7g was highly expressed during Follicle Atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.
Yi Wang - One of the best experts on this subject based on the ideXlab platform.
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expression and preliminary functional profiling of the let 7 family during porcine Ovary Follicle Atresia
Molecules and Cells, 2015Co-Authors: Rui Cao, Xiaolong Zhou, Peng Xiao, Yi Wang, Hong Lin LiuAbstract:Most Follicles in the mammalian Ovary undergo Atresia. Granulosa cell apoptosis is a hallmark of Follicle Atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular Atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) Ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in Follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine Ovary Follicles compared with healthy Follicles, while let-7g was highly expressed during Follicle Atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.