The Experts below are selected from a list of 17121 Experts worldwide ranked by ideXlab platform
S Almeria - One of the best experts on this subject based on the ideXlab platform.
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seroprevalence of toxoplasma gondii in wild pigs Sus Scrofa from spain
Veterinary Parasitology, 2005Co-Authors: C B L Gauss, Christian Gortazar, J P Dubey, Dolors Vidal, F Ruiz, J Vicente, Ignasi Marco, S Lavin, S AlmeriaAbstract:Sera collected from 507 hunter-killed wild pigs (Sus Scrofa) between 1993 and 2004 from five geographic regions in northern Spain and seven regions in southern Spain were assayed for antibodies to Toxoplasma gondii by the modified agglutination test (MAT). Antibodies to T. gondii were detected in 185 (38.4%) of 507 pigs with titers of 1:25 in 71, 1:50 in 111 and ≥1:500 in 3; seroprevalence was significantly higher (P < 0.05) in pigs from southern regions. Seroprevalence was density dependent; it was higher in pigs from high stocking per hectare and availability of forage. Statistically significant differences were not observed between T. gondii seroprevalence and hunting estates (open verSus fenced), sex or age. Serological results indicate a widespread exposure to T. gondii among Spanish wild boars, suggesting that this population could represent a public health risk for persons that handle or consume raw or undercooked infected wild pig meat.
Christian Gortazar - One of the best experts on this subject based on the ideXlab platform.
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genetic diversity of wild boar populations and domestic pig breeds Sus Scrofa in south western europe
Biological Journal of The Linnean Society, 2010Co-Authors: Paulo C Alves, Iris Pinheiro, Raquel Godinho, Joaquin Vicente, Christian Gortazar, Massimo ScanduraAbstract:The Eurasian wild boar, Sus Scrofa, is an important game species and is widely distributed in Eurasia. The recent demographic expansions allied to man mediated translocations and reintroductions, reshaped the genetic variability of wild boar populations, and gave rise to management problems. Genetic variability and the population structure of the European populations are not well-known, in particular in the Iberian Peninsula. In the present study, we assessed the phylogenetic relationship among 17 Iberian wild boar populations and several Eurasian wild and domestic pigs by sequencing 660 bp of the mitochondrial (mt)DNA control region. This analysis was extended to the two autochthonous Portuguese pig breeds, Bisaro and Alentejano, and their relationship with Eurasian pigs was also established. High levels of gene diversity were found in Iberian wild boar and Portuguese domestic pigs, as well as a significant genetic difference among and within populations. Iberian wild boar and Portuguese pig breeds belong to the main European clade but diverge with animals from Central Europe, showing a high proportion of private (Iberian) haplotypes. These results suggest the existence of glacial refugia for Sus Scrofa in the Iberian Peninsula during the Last Glacial Maximum. No signs of Asian mtDNA introgression from Asian populations were found in the present survey. © 2010 The Linnean Society of London, Biological Journal of the Linnean Society, 2010, 101, 797–822.
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seroprevalence of toxoplasma gondii in wild pigs Sus Scrofa from spain
Veterinary Parasitology, 2005Co-Authors: C B L Gauss, Christian Gortazar, J P Dubey, Dolors Vidal, F Ruiz, J Vicente, Ignasi Marco, S Lavin, S AlmeriaAbstract:Sera collected from 507 hunter-killed wild pigs (Sus Scrofa) between 1993 and 2004 from five geographic regions in northern Spain and seven regions in southern Spain were assayed for antibodies to Toxoplasma gondii by the modified agglutination test (MAT). Antibodies to T. gondii were detected in 185 (38.4%) of 507 pigs with titers of 1:25 in 71, 1:50 in 111 and ≥1:500 in 3; seroprevalence was significantly higher (P < 0.05) in pigs from southern regions. Seroprevalence was density dependent; it was higher in pigs from high stocking per hectare and availability of forage. Statistically significant differences were not observed between T. gondii seroprevalence and hunting estates (open verSus fenced), sex or age. Serological results indicate a widespread exposure to T. gondii among Spanish wild boars, suggesting that this population could represent a public health risk for persons that handle or consume raw or undercooked infected wild pig meat.
Celine Richomme - One of the best experts on this subject based on the ideXlab platform.
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environmental factors associated with the seroprevalence of toxoplasma gondii in wild boars Sus Scrofa france
Ecohealth, 2012Co-Authors: Marina Beral, Sophie Rossi, Dominique Aubert, Patrick Gasqui, Marieeve Terrier, Francois Klein, Isabelle Villena, David Abrial, Emmanuelle Gilotfromont, Celine RichommeAbstract:Toxoplasma gondii is a protozoan parasite infecting humans and animals. Wild boars Sus Scrofa are a potential source of human infection and an appropriate biological model for analyzing T. gondii dynamics in the environment. Here, we aimed to identify environmental factors explaining the seroprevalence of toxoplasmosis in French wild boar populations. Considering 938 individuals sampled from 377 ‘communes’, overall seroprevalence was 23% (95% confidence interval: [22–24]). Using a Poisson regression, we found that the number of seropositive wild boars detected per ‘commune’ was positively associated with the presence of European wildcats (Felis silvestris) and moderate winter temperatures.
C B L Gauss - One of the best experts on this subject based on the ideXlab platform.
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seroprevalence of toxoplasma gondii in wild pigs Sus Scrofa from spain
Veterinary Parasitology, 2005Co-Authors: C B L Gauss, Christian Gortazar, J P Dubey, Dolors Vidal, F Ruiz, J Vicente, Ignasi Marco, S Lavin, S AlmeriaAbstract:Sera collected from 507 hunter-killed wild pigs (Sus Scrofa) between 1993 and 2004 from five geographic regions in northern Spain and seven regions in southern Spain were assayed for antibodies to Toxoplasma gondii by the modified agglutination test (MAT). Antibodies to T. gondii were detected in 185 (38.4%) of 507 pigs with titers of 1:25 in 71, 1:50 in 111 and ≥1:500 in 3; seroprevalence was significantly higher (P < 0.05) in pigs from southern regions. Seroprevalence was density dependent; it was higher in pigs from high stocking per hectare and availability of forage. Statistically significant differences were not observed between T. gondii seroprevalence and hunting estates (open verSus fenced), sex or age. Serological results indicate a widespread exposure to T. gondii among Spanish wild boars, suggesting that this population could represent a public health risk for persons that handle or consume raw or undercooked infected wild pig meat.
Rosario Martin - One of the best experts on this subject based on the ideXlab platform.
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differentiation of european wild boar Sus Scrofa Scrofa and domestic swine Sus Scrofa domestica meats by pcr analysis targeting the mitochondrial d loop and the nuclear melanocortin receptor 1 mc1r genes
Meat Science, 2008Co-Authors: Violeta Fajardo, Isabel Gonzalez, Irene Martinez Martin, Mari A Rojas, Teresa Garci A, Pablo E. Hernández, Rosario MartinAbstract:Abstract This work describes the differentiation of European wild boar ( Sus Scrofa Scrofa ) and domestic swine ( Sus Scrofa domestica ) meats by PCR targeting sequences from two molecular markers: the mitochondrial displacement loop (D-loop) region and the nuclear melanocortin receptor 1 ( MC1R ) gene. A polymorphic D-loop fragment (∼270 bp) was amplified and sequenced in a number of wild and domestic Sus Scrofa meat samples, to find a nucleotide region suitable for PCR–RFLP analysis. Sequence data showed the presence of only a few point mutations across Sus Scrofa D-loop sequences, not allowing direct discrimination between wild boar and domestic swine meats. Later, the MC1R gene was targeted and Sus Scrofa -specific primers designed to amplify a 795 bp MC1R fragment. Subsequent RFLP analysis of the MC1R swine-specific amplicons allowed selection of BspHI and BstUI endonucleases to carry out intraspecific Sus Scrofa differentiation. Digestion of MC1R amplicons with the chosen enzymes generated characteristic PCR–RFLP profiles that allowed discrimination among meats from wild and domestic swine specimens. The technique also enabled the detection of samples that yielded heterozygous profiles, suggesting hybrids resulting from wild boar and domestic pig breeding. The PCR–RFLP reported here, targeting the MC1R gene may be routinely applied to verify the correct labelling of game products.
