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López Gatius Fernando - One of the best experts on this subject based on the ideXlab platform.
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Improved embryo survival following follicular drainage of subordinate follicles for twin Pregnancy prevention in bi-ovular dairy cows
Society for Reproduction and Development, 2020Co-Authors: García Ispierto Irina, López Gatius FernandoAbstract:To prevent twin pregnancies in cattle, a simple transvaginal device can be used for follicular drainage. This study examines embryo survival following follicular drainage of the smaller pre-ovulatory follicle at timed artificial insemination (AI) in cows with a pre-ovulatory follicle in each Ovary. The study groups established were a control group of 289 monovular cows, a non-drainage group of 114 bi-ovular cows and a follicular drainage group of 113 bi-ovular cows. All cows undergoing drainage developed a corpus luteum (CL) in the drained Ovary. Pregnancy loss was recorded 56 days post-AI in 19.5% of the 149 cows that became pregnant. Pregnancy loss in the drainage group cows not suffering heat stress (3.8%) was significantly lower (P < 0.05) than in control non-heat stressed cows (20.9%) and heat-stressed non-drainage group cows (25%). Results indicate that CL induction by follicular drainage for twin Pregnancy prevention may reduce the incidence of Pregnancy loss
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Improved embryo survival following follicular drainage of subordinate follicles for twin Pregnancy prevention in bi-ovular dairy cows
'Japanese Society of Animal Reproduction', 2020Co-Authors: García Ispierto Irina, López Gatius FernandoAbstract:To prevent twin pregnancies in cattle, a simple transvaginal device can be used for follicular drainage. This study examines embryo survival following follicular drainage of the smaller pre-ovulatory follicle at timed artificial insemination (AI) in cows with a pre-ovulatory follicle in each Ovary. The study groups established were a control group of 289 monovular cows, a non-drainage group of 114 bi-ovular cows and a follicular drainage group of 113 bi-ovular cows. All cows undergoing drainage developed a corpus luteum (CL) in the drained Ovary. Pregnancy loss was recorded 56 days post-AI in 19.5% of the 149 cows that became pregnant. Pregnancy loss in the drainage group cows not suffering heat stress (3.8%) was significantly lower (P < 0.05) than in control non-heat stressed cows (20.9%) and heat-stressed non-drainage group cows (25%). Results indicate that CL induction by follicular drainage for twin Pregnancy prevention may reduce the incidence of Pregnancy loss.This study received financial support from the procedure “01.02.01 de Transferència Tecnològica del Programa de desenvolupament rural de Catalunya 2014-2020” (Number 19005)
García Ispierto Irina - One of the best experts on this subject based on the ideXlab platform.
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Improved embryo survival following follicular drainage of subordinate follicles for twin Pregnancy prevention in bi-ovular dairy cows
Society for Reproduction and Development, 2020Co-Authors: García Ispierto Irina, López Gatius FernandoAbstract:To prevent twin pregnancies in cattle, a simple transvaginal device can be used for follicular drainage. This study examines embryo survival following follicular drainage of the smaller pre-ovulatory follicle at timed artificial insemination (AI) in cows with a pre-ovulatory follicle in each Ovary. The study groups established were a control group of 289 monovular cows, a non-drainage group of 114 bi-ovular cows and a follicular drainage group of 113 bi-ovular cows. All cows undergoing drainage developed a corpus luteum (CL) in the drained Ovary. Pregnancy loss was recorded 56 days post-AI in 19.5% of the 149 cows that became pregnant. Pregnancy loss in the drainage group cows not suffering heat stress (3.8%) was significantly lower (P < 0.05) than in control non-heat stressed cows (20.9%) and heat-stressed non-drainage group cows (25%). Results indicate that CL induction by follicular drainage for twin Pregnancy prevention may reduce the incidence of Pregnancy loss
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Improved embryo survival following follicular drainage of subordinate follicles for twin Pregnancy prevention in bi-ovular dairy cows
'Japanese Society of Animal Reproduction', 2020Co-Authors: García Ispierto Irina, López Gatius FernandoAbstract:To prevent twin pregnancies in cattle, a simple transvaginal device can be used for follicular drainage. This study examines embryo survival following follicular drainage of the smaller pre-ovulatory follicle at timed artificial insemination (AI) in cows with a pre-ovulatory follicle in each Ovary. The study groups established were a control group of 289 monovular cows, a non-drainage group of 114 bi-ovular cows and a follicular drainage group of 113 bi-ovular cows. All cows undergoing drainage developed a corpus luteum (CL) in the drained Ovary. Pregnancy loss was recorded 56 days post-AI in 19.5% of the 149 cows that became pregnant. Pregnancy loss in the drainage group cows not suffering heat stress (3.8%) was significantly lower (P < 0.05) than in control non-heat stressed cows (20.9%) and heat-stressed non-drainage group cows (25%). Results indicate that CL induction by follicular drainage for twin Pregnancy prevention may reduce the incidence of Pregnancy loss.This study received financial support from the procedure “01.02.01 de Transferència Tecnològica del Programa de desenvolupament rural de Catalunya 2014-2020” (Number 19005)
Robert H Young - One of the best experts on this subject based on the ideXlab platform.
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Pregnancy luteoma a study of 20 cases on the occasion of the 50th anniversary of its description by dr william h sternberg with an emphasis on the common presence of follicle like spaces and their diagnostic implications
The American Journal of Surgical Pathology, 2014Co-Authors: Eike Burandt, Robert H YoungAbstract:Twenty cases of the distinctive tumor-like lesion of the Ovary, Pregnancy luteoma, are described, with emphasis on pathologic features. The masses occurred in patients from 15 to 44 years of age and were typically incidental findings at or near term. Four patients experienced androgenic manifestations. The luteoma was documented to be bilateral in only 4 cases, but the opposite Ovary was usually not evaluated pathologically. They ranged up to 15 cm. Sectioning typically showed multiple nodules or a single discrete mass with a multinodular sectioned surface and a soft bulging appearance. Most lesions were brown, but a few were black or yellow at least focally. Foci of hemorrhage were common. The most common microscopic appearance was a diffuse growth, but it was punctuated in about three quarters of the cases by follicle-like spaces often containing colloid-like secretion. These spaces are much more characteristic of the Pregnancy luteoma than of the lesion most often in the differential diagnosis, a steroid cell tumor, and accordingly may be of diagnostic aid. However, they may cause confusion, as follicle-like spaces are a nonspecific feature of a number of neoplasms involving the Ovary and potentially in the differential diagnosis. Awareness of the clinical background and frequency of finding follicle-like spaces in Pregnancy luteoma are important diagnostically, and standard immunohistochemical stains will aid should they be warranted.
P Sanchez - One of the best experts on this subject based on the ideXlab platform.
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first birth of an animal from an extinct subspecies capra pyrenaica pyrenaica by cloning
Theriogenology, 2009Co-Authors: J Folch, M J Cocero, P Chesne, J L Alabart, V Dominguez, Y Cognie, A Roche, A Fernandezarias, J I Marti, P SanchezAbstract:Abstract Two experiments have been performed to clone the bucardo, an extinct wild goat. The karyoplasts were thawed fibroblasts derived from skin biopsies, obtained and cryopreserved in 1999 from the last living specimen, a female, which died in 2000. Cytoplasts were mature oocytes collected from the oviducts of superovulated domestic goats. Oocytes were enucleated and coupled to bucardo's fibroblasts by electrofusion. Reconstructed embryos were cultured for 36 h or 7 d and transferred to either Spanish ibex or hybrid (Spanish ibex male × domestic goat) synchronized recipients. Embryos were placed, according to their developmental stage, into the oviduct or into the uterine horn ipsilateral to an ovulated Ovary. Pregnancy was monitored through their plasmatic PAG levels. In Experiment 1, 285 embryos were reconstructed and 30 of them were transferred at the 3- to 6-cells stage to 5 recipients. The remaining embryos were further cultured to day 7, and 24 of them transferred at compact morula/blastocyst stage to 8 recipients. In Experiment 2, 154 reconstructed embryos were transferred to 44 recipients at the 3- to 6-cells stage. Pregnancies were attained in 0/8 and 7/49 of the uterine and oviduct-transferred recipients, respectively. One recipient maintained Pregnancy to term, displaying very high PAG levels. One morphologically normal bucardo female was obtained by caesarean section. The newborn died some minutes after birth due to physical defects in lungs. Nuclear DNA confirmed that the clone was genetically identical to the bucardo's donor cells. To our knowledge, this is the first animal born from an extinct subspecies.
Eike Burandt - One of the best experts on this subject based on the ideXlab platform.
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Pregnancy luteoma a study of 20 cases on the occasion of the 50th anniversary of its description by dr william h sternberg with an emphasis on the common presence of follicle like spaces and their diagnostic implications
The American Journal of Surgical Pathology, 2014Co-Authors: Eike Burandt, Robert H YoungAbstract:Twenty cases of the distinctive tumor-like lesion of the Ovary, Pregnancy luteoma, are described, with emphasis on pathologic features. The masses occurred in patients from 15 to 44 years of age and were typically incidental findings at or near term. Four patients experienced androgenic manifestations. The luteoma was documented to be bilateral in only 4 cases, but the opposite Ovary was usually not evaluated pathologically. They ranged up to 15 cm. Sectioning typically showed multiple nodules or a single discrete mass with a multinodular sectioned surface and a soft bulging appearance. Most lesions were brown, but a few were black or yellow at least focally. Foci of hemorrhage were common. The most common microscopic appearance was a diffuse growth, but it was punctuated in about three quarters of the cases by follicle-like spaces often containing colloid-like secretion. These spaces are much more characteristic of the Pregnancy luteoma than of the lesion most often in the differential diagnosis, a steroid cell tumor, and accordingly may be of diagnostic aid. However, they may cause confusion, as follicle-like spaces are a nonspecific feature of a number of neoplasms involving the Ovary and potentially in the differential diagnosis. Awareness of the clinical background and frequency of finding follicle-like spaces in Pregnancy luteoma are important diagnostically, and standard immunohistochemical stains will aid should they be warranted.