The Experts below are selected from a list of 39 Experts worldwide ranked by ideXlab platform
Gary J Killian - One of the best experts on this subject based on the ideXlab platform.
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Effect of bovine ampullary and isthmic Oviductal fluid on motility, acrosome reaction and fertility of bull spermatozoa
Reproduction, 1995Co-Authors: Anne A. Grippo, Gary J KillianAbstract:Motility, acrosome reaction and oocyte fertilizing ability were assessed for bull spermatozoa after incubation in regional (isthmic or ampullary), bovine Oviductal fluid, pooled by stage of the oestrous cycle. Oviductal Fluids collected daily from isthmic and ampullary cannulae implanted in the same oviduct were divided into pools, representing two oestrous cycle stages, based on daily serum progesterone concentrations. Ejaculated bull spermatozoa were incubated for 0\p=n-\6h in each type of Oviductal fluid. Incubation in isthmic Oviductal fluid collected during the nonluteal stage, including oestrus and ovulation, decreased overall sperm motility (from 71.7% motile spermatozoa to 34.0% and both path (78 \g=m\ms \m=-\1versus 86\p=n-\89\g=m\m s\m=-\1) and progressive (74 \g=m\ms\m=-\1versus 83\p=n-\85\g=m\m s\m=-\1) velocities of spermatozoa motion. Spermatozoa incubated in isthmic, non-luteal Oviductal fluid had a higher rate and extent of sperm acrosome reaction (213% of control versus 136\p=n-\161%of control by 2 h incubation) compared with spermatozoa incubated in other Oviductal fluid types. However, incubation in nonluteal ampullary fluid increased the number of spermatozoa, which were both acrosome reacted and live, and able to fertilize bovine ova (88.7% fertilized versus 75\p=n-\81%).Glycosaminoglycan concentrations were similar among types of Oviductal fluid (0.77\p=n-\0.88mg ml \m=-\1). These findings indicate that Oviductal fluid differentially affects sperm function, depending on the oviduct region and the stage of the oestrous cycle at which the fluid was obtained.
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effect of follicular or Oviductal Fluids on movement characteristics of bovine sperm during capacitation in vitro
Journal of Andrology, 1994Co-Authors: Tamara L Mcnutt, Patricia Oldsclarke, Susan S Suarez, Gary J KillianAbstract:: Mammalian sperm exhibit characteristic motility changes associated with capacitation. Movement characteristics of bovine sperm incubated in noncapacitating (control, medium alone), capacitating (oviduct fluid, nonluteal, and luteal), or capacitating, acrosome reaction inducing (follicular fluid) conditions were investigated using a computer-assisted automated semen analysis system. Sperm were incubated up to 4 hours in a modified Tyrode's medium (control), 20 and 60% nonluteal (NL) or luteal (L) oviduct fluid (ODF), or 20 and 60% follicular fluid (FF). Relative to sperm incubated in control medium, motility of sperm treated with ODF or FF had increased linearity and vigorous motility. Sperm incubated in 60% ODF or FF showed a small decrease in mean trajectory/path straightness and velocity over time compared to 20% fluid treatments and control. Frequency distribution graphs were symmetric for 20% NL- and L-ODF treated sperm. However, 20% FF and 60% ODF and FF treatments had distributions skewed to the left, indicating smaller values for lateral head displacement (ALH) and curvilinear velocity (VOL). Me dian values for ALH and VCL were determined for control-treated sperm, and subtracted from individual sperm values for all treatments to estimate deviation from control, designated ALHc and VCLc. Three-dimensional plots of ALHc, VCLc and corresponding frequency indicated shifts in peak patterns for fluid-treated sperm compared to control sperm. Incubation in 20% ODF and FF resulted in peak shift for ALH and VCL values; yet, little change in peak position was observed in sperm incubated in 60% ODF and FF. Although mean values decreased for ALH and VCL during the 4-hour incubation, three-dimensional plots suggested an increase in the frequency of individual sperm with these parameters. This study provides additional information on bovine sperm motion during in vitro capacitation and indicates that the presence of ODF and FF influences sperm motion characteristics.
L C Giojalas - One of the best experts on this subject based on the ideXlab platform.
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increased velocity and induction of chemotactic response in mouse spermatozoa by follicular and Oviductal Fluids
Reproduction, 1999Co-Authors: R G Oliveira, L Tomasi, R A Rovasio, L C GiojalasAbstract:: The dynamic parameters of mouse sperm cells exposed to follicular and Oviductal Fluids were assessed. Spermatozoa were tracked on a chemotactic Zigmond chamber and recorded using a videomicroscopy system. The results were evaluated with computer-supported image analysis. Follicular fluid at a dilution of 10(-4) markedly increased the proportion of spermatozoa with high velocity, and stimulated chemotactic behaviour. The highest velocities were observed in sperm cells exposed to Oviductal fluid, and a greater proportion of these cells had high velocity compared with those exposed to follicular fluid. Chemotaxis was induced in spermatozoa exposed to Oviductal fluid at dilutions of 10(-3) and 10(-5). These results suggest the presence of temporal subpopulations of responsive spermatozoa, considering the distance travelled towards both follicular and Oviductal Fluids and the proportion of sperm cells migrating towards the gradient in the highest distance ranges. This is the first report on the effect of isolated follicular and Oviductal Fluids on dynamic parameters and chemotaxis of mouse spermatozoa. The findings support previous work showing that the motility and directionality of mouse sperm cells is increased by factors in the microenvironment of the egg. Although the significance of these factors in vivo is unknown, it is possible that there is a relay mechanism involving sequential activity of both Oviductal and follicular Fluids to direct the male gametes towards the egg.
R G Oliveira - One of the best experts on this subject based on the ideXlab platform.
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increased velocity and induction of chemotactic response in mouse spermatozoa by follicular and Oviductal Fluids
Reproduction, 1999Co-Authors: R G Oliveira, L Tomasi, R A Rovasio, L C GiojalasAbstract:: The dynamic parameters of mouse sperm cells exposed to follicular and Oviductal Fluids were assessed. Spermatozoa were tracked on a chemotactic Zigmond chamber and recorded using a videomicroscopy system. The results were evaluated with computer-supported image analysis. Follicular fluid at a dilution of 10(-4) markedly increased the proportion of spermatozoa with high velocity, and stimulated chemotactic behaviour. The highest velocities were observed in sperm cells exposed to Oviductal fluid, and a greater proportion of these cells had high velocity compared with those exposed to follicular fluid. Chemotaxis was induced in spermatozoa exposed to Oviductal fluid at dilutions of 10(-3) and 10(-5). These results suggest the presence of temporal subpopulations of responsive spermatozoa, considering the distance travelled towards both follicular and Oviductal Fluids and the proportion of sperm cells migrating towards the gradient in the highest distance ranges. This is the first report on the effect of isolated follicular and Oviductal Fluids on dynamic parameters and chemotaxis of mouse spermatozoa. The findings support previous work showing that the motility and directionality of mouse sperm cells is increased by factors in the microenvironment of the egg. Although the significance of these factors in vivo is unknown, it is possible that there is a relay mechanism involving sequential activity of both Oviductal and follicular Fluids to direct the male gametes towards the egg.
Marie Saint-dizier - One of the best experts on this subject based on the ideXlab platform.
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Regulation of the bovine Oviductal fluid proteome.
Reproduction, 2016Co-Authors: Julie Lamy, Valérie Labas, Grégoire Harichaux, Guillaume Tsikis, Pascal Mermillod, Marie Saint-dizierAbstract:Our objective was to investigate the regulation of the proteome in the bovine Oviductal fluid according to the stage of the oestrous cycle, to the side relative to ovulation and to local concentrations of steroid hormones. Luminal fluid samples from both oviducts were collected at four stages of the oestrous cycle: pre-ovulatory (Pre-ov), post-ovulatory (Post-ov), and mid- and late luteal phases from adult cyclic cows (18-25 cows/stage). The proteomes were assessed by nanoLC-MS/MS and quantified by label-free method. Totally, 482 proteins were identified including a limited number of proteins specific to one stage or one side. Proportions of differentially abundant proteins fluctuated from 10 to 24% between sides at one stage and from 4 to 20% among stages in a given side of ovulation. In Oviductal Fluids ipsilateral to ovulation, Annexin A1 was the most abundant protein at Pre-ov compared with Post-ov while numerous heat shock proteins were more abundant at Post-ov compared with Pre-ov. Among differentially abundant proteins, seven tended to be correlated with intra-Oviductal concentrations of progesterone. A wide range of biological processes was evidenced for differentially abundant proteins, of which metabolic and cellular processes were predominant. This work identifies numerous new candidate proteins potentially interacting with the oocyte, spermatozoa and embryo to modulate fertilization and early embryo development.
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A proteomic approach to monitor interactions between Oviductal fluid and spermatozoa across the estrous cycle
Animal reproduction, 2016Co-Authors: P. Nogues, Valérie Labas, Guillaume Tsikis, Pascal Mermillod, J. Lamy, L. Combes, Xavier Druart, Marie Saint-dizierAbstract:In the bovine during estrus (pre-ovulatory period), both oviducts are able to maintain sperm viability in the so called “sperm reservoir” with the assumed aim of preserving sperm viability up to the time of ovulation. However, very few molecules interacting with bull spermatozoa (spz) and playing a role in the maintenance of bull sperm viability in vivo were identified. Furthermore, the effect of the stage of the estrous cycle on sperm-oviduct interactions is barely known. The aim of this study was to identify proteins present in the Oviductal fluid (OF) that interact with spz and to determine how stage of cycle affects these interactions. Bovine Oviductal Fluids (OF) were collected at a local slaughterhouse at 3 stages of the estrous cycle based on ovarian morphology: before ovulation (Pre-ov; preovulatory follicle, corpus albicans), after ovulation (Post-ov; corpus hemorragicum) and during the luteal phase (Lut; corpus luteum), and stored at −80°C until used. Frozenthawed spermatozoa (spz) from one bull were washed then incubated at a final concentration of 330.106 spz/ml in phosphate-buffered saline supplemented with 14% OF (7 mg proteins/ml, based on a positive effect of OF at this concentration on sperm viability) at one of the three stages or a protein-free medium (Synthetic Oviductal fluid or SOF, control group) for 1h at 37°C, then washed again. Total proteins from spz were migrated on a 10% SDSPAGE and each lane was divided in 3 bands for in-gel digestion before proteomic analysis by nano LC-MS/MS. Proteins were considered to originate from the OF and to interact with spz when detected in at least one treatment group but not in the control group. Normalized spectral counts of interacting proteins were compared between stages with T-tests and considered differential when P 2 or
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69 DIFFERENTIAL AND QUANTITATIVE ANALYSIS OF DOG Oviductal FLUID
Reproduction Fertility and Development, 2012Co-Authors: Karine Reynaud, Valérie Labas, Grégoire Harichaux, Sandra Thoumire, Muhammad Tahir, Sylvie Chastant-maillard, Marie Saint-dizierAbstract:The major reproductive peculiarity of the bitch is that ovulation releases prophase I (germinal vesicle, GV, immature) oocytes. Resumption of meiotic maturation, as well as fertilisation and embryonic development to the morula stage occur in the oviduct. Because the dog is a biomedical model for human diseases and also a model for endangered canid species, the development of assisted reproduction techniques would be of great interest. To date, in vitro-produced canine embryos remain exceptional and no puppy has been born. The main limiting factors of in vitro embryo production are the low oocyte maturation rates, the poor oocyte quality and the high polyspermy. A better knowledge of the composition of Oviductal fluid during the periovulatory period may help to mimic the in vivo conditions for in vitro oocyte culture and, thereafter, their fertilisation and embryonic development. The objective of this study was to analyse the Oviductal fluid by a label-free quantitative proteomic workflow based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) protein separation, nano-scale liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) analysis and quantitative method using spectral counting. Ovarian cycles were followed by vaginal smears, ultrasonography and progesterone blood assays. Oviductal Fluids were collected from 3 beagle bitches, after ovariectomies performed 3.5 days after ovulation. After dissection, the ampulla and isthmus were separated and flushed with 50 μL of PBS. Oviductal Fluids were submitted to 1D SDS-PAGE and all bands were digested with trypsin. Peptide extracts were analysed on an Ettan multidimensional LC (MDLC) system coupled to a linear ion trap quadrupole (LTQ) mass spectrometer. After protein identification using Mascot server and with Swiss-Prot and National Center for Biotechnology Information (NCBI) databases, bioinformatic processing of data and statistic analysis (t-test with P
Tamara L Mcnutt - One of the best experts on this subject based on the ideXlab platform.
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effect of follicular or Oviductal Fluids on movement characteristics of bovine sperm during capacitation in vitro
Journal of Andrology, 1994Co-Authors: Tamara L Mcnutt, Patricia Oldsclarke, Susan S Suarez, Gary J KillianAbstract:: Mammalian sperm exhibit characteristic motility changes associated with capacitation. Movement characteristics of bovine sperm incubated in noncapacitating (control, medium alone), capacitating (oviduct fluid, nonluteal, and luteal), or capacitating, acrosome reaction inducing (follicular fluid) conditions were investigated using a computer-assisted automated semen analysis system. Sperm were incubated up to 4 hours in a modified Tyrode's medium (control), 20 and 60% nonluteal (NL) or luteal (L) oviduct fluid (ODF), or 20 and 60% follicular fluid (FF). Relative to sperm incubated in control medium, motility of sperm treated with ODF or FF had increased linearity and vigorous motility. Sperm incubated in 60% ODF or FF showed a small decrease in mean trajectory/path straightness and velocity over time compared to 20% fluid treatments and control. Frequency distribution graphs were symmetric for 20% NL- and L-ODF treated sperm. However, 20% FF and 60% ODF and FF treatments had distributions skewed to the left, indicating smaller values for lateral head displacement (ALH) and curvilinear velocity (VOL). Me dian values for ALH and VCL were determined for control-treated sperm, and subtracted from individual sperm values for all treatments to estimate deviation from control, designated ALHc and VCLc. Three-dimensional plots of ALHc, VCLc and corresponding frequency indicated shifts in peak patterns for fluid-treated sperm compared to control sperm. Incubation in 20% ODF and FF resulted in peak shift for ALH and VCL values; yet, little change in peak position was observed in sperm incubated in 60% ODF and FF. Although mean values decreased for ALH and VCL during the 4-hour incubation, three-dimensional plots suggested an increase in the frequency of individual sperm with these parameters. This study provides additional information on bovine sperm motion during in vitro capacitation and indicates that the presence of ODF and FF influences sperm motion characteristics.
