The Experts below are selected from a list of 36 Experts worldwide ranked by ideXlab platform
Ákos Boros - One of the best experts on this subject based on the ideXlab platform.
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Identification of a novel Astrovirus in domestic sheep in Hungary
Archives of Virology, 2011Co-Authors: Gábor Reuter, Péter Pankovics, Eric Delwart, Ákos BorosAbstract:The family Astroviridae consists of two genera, AvAstrovirus and MamAstrovirus, whose members are associated with gastroenteritis in avian and mammalian hosts, respectively. We serendipitously identified a novel Ovine Astrovirus in a fecal specimen from a domestic sheep (Ovis aries) in Hungary by viral metagenomic analysis. Sequencing of the fragment indicated that it was an ORF1b/ORF2/3′UTR sequence, and it has been submitted to the GenBank database as Ovine Astrovirus type 2 (OAstV-2/Hungary/2009) with accession number JN592482. The unique sequence characteristics and the phylogenetic position of OAstV-2 suggest that genetically divergent lineages of Astroviruses exist in sheep.
Martin Beer - One of the best experts on this subject based on the ideXlab platform.
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A novel Astrovirus associated with encephalitis and ganglionitis in domestic sheep.
Transboundary and Emerging Diseases, 2017Co-Authors: Florian Pfaff, Kore Schlottau, S. F. E. Scholes, A. E. Courtenay, Bernd Hoffmann, Dirk Höper, Martin BeerAbstract:In June 2013, a 4-year-old Welsh Mountain ewe and in March 2014 a 10-day-old lamb of the same breed and the same flock presented progressive neurological signs including depressed sensorium, tremor, and unusual behaviour. Neuropathological examination of the brain and spinal cord detected non-suppurative polioencephalomyelitis and dorsal root ganglionitis, characteristic of a neurotropic viral agent in both sheep. Metagenomic analysis of different tissue samples from both animals identified a novel Ovine Astrovirus (OvAstV). The presence of viral genome in the central nervous system was confirmed by RT-qPCR. Although the cases presented nine months apart, the identified OvAstV shared nearly identical sequences, differing in only three nucleotide positions across the complete genome. Phylogenetic analysis revealed a close relation of OvAstV to neurotropic bOvine Astroviruses and an enteric OvAstV. In conclusion, these are the first reported cases of Astrovirus infection in domestic sheep that were associated with encephalitis and ganglionitis.
Tsuneyuki Masuda - One of the best experts on this subject based on the ideXlab platform.
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Full genome analysis of bOvine Astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of bOvine Astrovirus
Archives of Virology, 2015Co-Authors: Makoto Nagai, Tsutomu Omatsu, Hiroshi Aoki, Konosuke Otomaru, Takehiko Uto, Motoya Koizumi, Fujiko Minami-fukuda, Hikaru Takai, Toshiaki Murakami, Tsuneyuki MasudaAbstract:A viral metagenomics approach was used to investigate fecal samples of Japanese calves with and without diarrhea. Of the different viral pathogens detected, read counts gave nearly complete Astrovirus-related RNA sequences in 15 of the 146 fecal samples collected in three distinct areas (Hokkaido, Ishikawa, and Kagoshima Prefectures) between 2009 and 2015. Due to the lack of genetic information about bOvine Astroviruses (BoAstVs) in Japan, these sequences were analyzed in this study. Nine of the 15 Japanese BoAstVs were closely related to Chinese BoAstVs and clustered into a lineage (tentatively named lineage 1) in all phylogenetic trees. Three of 15 strains were phylogenetically separate from lineage 1, showing low sequence identities, and clustered instead with an American strain isolated from cattle with respiratory disease (tentatively named lineage 2). Interestingly, two of 15 strains clustered with lineage 1 in the open reading frame (ORF)1a and ORF1b regions, while they clustered with lineage 2 in the ORF2 region. Remarkably, one of 15 strains exhibited low amino acid sequence similarity to other BoAstVs and was clustered separately with porcine Astrovirus type 5 in all trees, and Ovine Astrovirus in the ORF2 region, suggesting past interspecies transmission.
Gábor Reuter - One of the best experts on this subject based on the ideXlab platform.
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Identification of a novel Astrovirus in domestic sheep in Hungary
Archives of Virology, 2011Co-Authors: Gábor Reuter, Péter Pankovics, Eric Delwart, Ákos BorosAbstract:The family Astroviridae consists of two genera, AvAstrovirus and MamAstrovirus, whose members are associated with gastroenteritis in avian and mammalian hosts, respectively. We serendipitously identified a novel Ovine Astrovirus in a fecal specimen from a domestic sheep (Ovis aries) in Hungary by viral metagenomic analysis. Sequencing of the fragment indicated that it was an ORF1b/ORF2/3′UTR sequence, and it has been submitted to the GenBank database as Ovine Astrovirus type 2 (OAstV-2/Hungary/2009) with accession number JN592482. The unique sequence characteristics and the phylogenetic position of OAstV-2 suggest that genetically divergent lineages of Astroviruses exist in sheep.
Florian Pfaff - One of the best experts on this subject based on the ideXlab platform.
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A novel Astrovirus associated with encephalitis and ganglionitis in domestic sheep.
Transboundary and Emerging Diseases, 2017Co-Authors: Florian Pfaff, Kore Schlottau, S. F. E. Scholes, A. E. Courtenay, Bernd Hoffmann, Dirk Höper, Martin BeerAbstract:In June 2013, a 4-year-old Welsh Mountain ewe and in March 2014 a 10-day-old lamb of the same breed and the same flock presented progressive neurological signs including depressed sensorium, tremor, and unusual behaviour. Neuropathological examination of the brain and spinal cord detected non-suppurative polioencephalomyelitis and dorsal root ganglionitis, characteristic of a neurotropic viral agent in both sheep. Metagenomic analysis of different tissue samples from both animals identified a novel Ovine Astrovirus (OvAstV). The presence of viral genome in the central nervous system was confirmed by RT-qPCR. Although the cases presented nine months apart, the identified OvAstV shared nearly identical sequences, differing in only three nucleotide positions across the complete genome. Phylogenetic analysis revealed a close relation of OvAstV to neurotropic bOvine Astroviruses and an enteric OvAstV. In conclusion, these are the first reported cases of Astrovirus infection in domestic sheep that were associated with encephalitis and ganglionitis.
