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Morici G - One of the best experts on this subject based on the ideXlab platform.
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Oxygen-Haemoglobin Dissociation Curve in hypoxic rats of first or second generation
Clinical and experimental pharmacology & physiology, 1993Co-Authors: Quatrini U, Licciardi A, Morici GAbstract:SUMMARY 1. Albino Wistar rats were raised in a normobaric hypoxic environment (10% O2 in N2). Two generations of hypoxic rats were observed for changes in their haemoglobin-oxygen (Hb-O2) Dissociation Curves (ODC), 2,3-diphosphoglycerate (2,3-DPG), haemoglobin (Hb), and Hill coefficients at P50 (n50). The first generation were called (H1) and the second generation (H2). The control group (N) had a normoxic environment. 2. Thirty-five rats (13 N rats, 12 H1 rats and 10 H2 rats) were used. The 2,3-DPG was significantly higher in both hypoxic groups when compared with N rats (2.02 ± 0.51 mmol/L) but 2,3-DPG of H2 rats was significantly lower than that of H1 rats (H1 = 3.48±0.58 mmol/L and H2 = 2.76±0.54 mmol/L). The haemoglobin values were N = 2.00±0.26, H1 = 2.65±0.32and H2 = 2.36±0.30 mmol/L, respectively. 3. We observed considerable differences in Hb-O2 affinity between the three groups of rats. In standard conditions (pH = 7.400; pCO2= 40 mmHg at 37°C) the H1 rats showed a significantly decreased Hb-O2 affinity (P50, st= 37.0 ± 1.3 mmHg) when compared with both H2 and N rats; the H2 rats showed a significantly increased Hb-02 affinity (P50, st 1 31.1 ± 1.5 mmHg) when compared with controls N (P50, st= 34.7±2.1 mmHg). There were no significant differences in n50 values: N = 2.88 ± 0.44; H1 = 2.88 ± 0.77; and H2 = 2.94 ± 0.67. 4. Therefore, the H1 rats’ ODC was located well to the right of that of N rats; and the H2 rats had an ODC located to the left when compared with both HI and N rats. It is likely that in H2 rats the adaptive processes to the new environment are more advanced; therefore, the magnitude of the compensatory mechanisms required to maintain a sufficient tissue oxygenation may be reduced.
D.j. Pepple - One of the best experts on this subject based on the ideXlab platform.
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In vitro effect of dibenzyl trisulfide on the p50 of the oxygen haemoglobin Dissociation Curve.
Journal of basic and clinical physiology and pharmacology, 2020Co-Authors: D.j. Pepple, Darien-andrew Theodore Marsh, Marsha-lyn MckoyAbstract:Objectives Dibenzyl trisulfide (DTS) has been reported to have cytotoxic and anti-inflammatory effects. It also affects erythrocyte deformability. We investigated the effects of DTS on the p50 of the oxygen haemoglobin Dissociation Curve. Methods Blood samples from 10 healthy male volunteers with normal haemoglobin AA were exposed to 50, 100, 200 and 400 ng/mL, respectively, of DTS. A hemox-analyzer was used to obtain the p50 values. Results The mean p50 value for the control samples was 25.89 ± 2.18 mm Hg. The values for the samples exposed to 50, 100, 200 and 400 ng/mL were 23.53 ± 1.81 mm Hg, 22.62 ± 1.61 mm Hg, 21.88 ± 1.67 mm Hg and 21.68 ± 1.88 mm Hg, respectively. Conclusions DTS caused a significant (p
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The effect of delta-9-tetrahydrocannabinol and cannabidiol on p50 of the oxygen haemoglobin Dissociation Curve.
Fitoterapia, 2020Co-Authors: Tameika R. James, Charah T. Watson, D.j. PeppleAbstract:Abstract Cannabis is one of the most chemically complex plants containing a large number of compounds, among which are cannabinoids; the main biologically active constituents of the plant. Delta-9-tetrahydrocannabinol (THC) is known as the main psychoactive cannabinoid. Studies have shown that the effects of THC can be modulated by cannabidiol (CBD), another major cannabinoid which has several beneficial effects of its own. This study aims to look at the effect of different concentrations of THC and CBD separately and in combination on the release of oxygen from erythrocytes by measuring the p50 of the oxygen haemoglobin Dissociation Curve. Blood samples were collected from twenty four healthy adult male and female non-smokers. P50 values were measured using the Hemox Analyzer for different concentrations (0, 2.5, 25, 50 and 100 ng/ml) of THC and CBD respectively, as well as with combinations of THC and CBD in 4:1 and 1:1 ratios. The results indicate that there is a decrease in P50 with increasing concentrations of both THC and CBD separately and in combination. The decrease in P50 was significant (p The changes observed with increasing concentrations of both THC and CBD suggests that they have an increased affinity for oxygen. This may have implications for individuals who consistently use cannabis.
Quatrini U - One of the best experts on this subject based on the ideXlab platform.
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Oxygen-Haemoglobin Dissociation Curve in hypoxic rats of first or second generation
Clinical and experimental pharmacology & physiology, 1993Co-Authors: Quatrini U, Licciardi A, Morici GAbstract:SUMMARY 1. Albino Wistar rats were raised in a normobaric hypoxic environment (10% O2 in N2). Two generations of hypoxic rats were observed for changes in their haemoglobin-oxygen (Hb-O2) Dissociation Curves (ODC), 2,3-diphosphoglycerate (2,3-DPG), haemoglobin (Hb), and Hill coefficients at P50 (n50). The first generation were called (H1) and the second generation (H2). The control group (N) had a normoxic environment. 2. Thirty-five rats (13 N rats, 12 H1 rats and 10 H2 rats) were used. The 2,3-DPG was significantly higher in both hypoxic groups when compared with N rats (2.02 ± 0.51 mmol/L) but 2,3-DPG of H2 rats was significantly lower than that of H1 rats (H1 = 3.48±0.58 mmol/L and H2 = 2.76±0.54 mmol/L). The haemoglobin values were N = 2.00±0.26, H1 = 2.65±0.32and H2 = 2.36±0.30 mmol/L, respectively. 3. We observed considerable differences in Hb-O2 affinity between the three groups of rats. In standard conditions (pH = 7.400; pCO2= 40 mmHg at 37°C) the H1 rats showed a significantly decreased Hb-O2 affinity (P50, st= 37.0 ± 1.3 mmHg) when compared with both H2 and N rats; the H2 rats showed a significantly increased Hb-02 affinity (P50, st 1 31.1 ± 1.5 mmHg) when compared with controls N (P50, st= 34.7±2.1 mmHg). There were no significant differences in n50 values: N = 2.88 ± 0.44; H1 = 2.88 ± 0.77; and H2 = 2.94 ± 0.67. 4. Therefore, the H1 rats’ ODC was located well to the right of that of N rats; and the H2 rats had an ODC located to the left when compared with both HI and N rats. It is likely that in H2 rats the adaptive processes to the new environment are more advanced; therefore, the magnitude of the compensatory mechanisms required to maintain a sufficient tissue oxygenation may be reduced.
Licciardi A - One of the best experts on this subject based on the ideXlab platform.
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Oxygen-Haemoglobin Dissociation Curve in hypoxic rats of first or second generation
Clinical and experimental pharmacology & physiology, 1993Co-Authors: Quatrini U, Licciardi A, Morici GAbstract:SUMMARY 1. Albino Wistar rats were raised in a normobaric hypoxic environment (10% O2 in N2). Two generations of hypoxic rats were observed for changes in their haemoglobin-oxygen (Hb-O2) Dissociation Curves (ODC), 2,3-diphosphoglycerate (2,3-DPG), haemoglobin (Hb), and Hill coefficients at P50 (n50). The first generation were called (H1) and the second generation (H2). The control group (N) had a normoxic environment. 2. Thirty-five rats (13 N rats, 12 H1 rats and 10 H2 rats) were used. The 2,3-DPG was significantly higher in both hypoxic groups when compared with N rats (2.02 ± 0.51 mmol/L) but 2,3-DPG of H2 rats was significantly lower than that of H1 rats (H1 = 3.48±0.58 mmol/L and H2 = 2.76±0.54 mmol/L). The haemoglobin values were N = 2.00±0.26, H1 = 2.65±0.32and H2 = 2.36±0.30 mmol/L, respectively. 3. We observed considerable differences in Hb-O2 affinity between the three groups of rats. In standard conditions (pH = 7.400; pCO2= 40 mmHg at 37°C) the H1 rats showed a significantly decreased Hb-O2 affinity (P50, st= 37.0 ± 1.3 mmHg) when compared with both H2 and N rats; the H2 rats showed a significantly increased Hb-02 affinity (P50, st 1 31.1 ± 1.5 mmHg) when compared with controls N (P50, st= 34.7±2.1 mmHg). There were no significant differences in n50 values: N = 2.88 ± 0.44; H1 = 2.88 ± 0.77; and H2 = 2.94 ± 0.67. 4. Therefore, the H1 rats’ ODC was located well to the right of that of N rats; and the H2 rats had an ODC located to the left when compared with both HI and N rats. It is likely that in H2 rats the adaptive processes to the new environment are more advanced; therefore, the magnitude of the compensatory mechanisms required to maintain a sufficient tissue oxygenation may be reduced.
Tameika R. James - One of the best experts on this subject based on the ideXlab platform.
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The effect of delta-9-tetrahydrocannabinol and cannabidiol on p50 of the oxygen haemoglobin Dissociation Curve.
Fitoterapia, 2020Co-Authors: Tameika R. James, Charah T. Watson, D.j. PeppleAbstract:Abstract Cannabis is one of the most chemically complex plants containing a large number of compounds, among which are cannabinoids; the main biologically active constituents of the plant. Delta-9-tetrahydrocannabinol (THC) is known as the main psychoactive cannabinoid. Studies have shown that the effects of THC can be modulated by cannabidiol (CBD), another major cannabinoid which has several beneficial effects of its own. This study aims to look at the effect of different concentrations of THC and CBD separately and in combination on the release of oxygen from erythrocytes by measuring the p50 of the oxygen haemoglobin Dissociation Curve. Blood samples were collected from twenty four healthy adult male and female non-smokers. P50 values were measured using the Hemox Analyzer for different concentrations (0, 2.5, 25, 50 and 100 ng/ml) of THC and CBD respectively, as well as with combinations of THC and CBD in 4:1 and 1:1 ratios. The results indicate that there is a decrease in P50 with increasing concentrations of both THC and CBD separately and in combination. The decrease in P50 was significant (p The changes observed with increasing concentrations of both THC and CBD suggests that they have an increased affinity for oxygen. This may have implications for individuals who consistently use cannabis.
