The Experts below are selected from a list of 1401 Experts worldwide ranked by ideXlab platform
Zhihong Jiang - One of the best experts on this subject based on the ideXlab platform.
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quantitative comparison and metabolite profiling of saponins in different parts of the root of Panax Notoginseng
Journal of Agricultural and Food Chemistry, 2014Co-Authors: Jing Rong Wang, Zhihong Jiang, Leefong Yau, Weina Gao, Yong Liu, Puiwing Yick, Liang LiuAbstract:Although both rhizome and root of Panax Notoginseng are officially utilized as Notoginseng in “Chinese Pharmacopoeia”, individual parts of the root were differently used in practice. To provide chemical evidence for the differentiated usage, quantitative comparison and metabolite profiling of different portions derived from the whole root, as well as commercial samples, were carried out, showing an overall higher content of saponins in rhizome, followed by main root, branch root, and fibrous root. Ginsenoside Rb2 was proposed as a potential marker with a content of 0.5 mg/g as a threshold value for differentiating rhizome from other parts. Multivariate analysis of the metabolite profile further suggested 32 saponins as potential markers for the discrimination of different parts of Notoginseng. Collectively, the study provided comprehensive chemical evidence for the distinct usage of different parts of Notoginseng and, hence, is of great importance for the rational application and exploitation of individua...
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dammarane type triterpene saponins from the flowers of Panax Notoginseng
Molecules, 2009Co-Authors: Jing Rong Wang, Yuko Yamasaki, Takashi Tanaka, Isao Kouno, Zhihong JiangAbstract:Four new dammarane-type triterpene saponins named floranotoginsenosides A (1), B (2), C (3) and D (4), together with five known triterpene saponins, were isolated from the flowers of Panax Notoginseng. Their structures were elucidated on the basis of spectral and chemical evidence.
Hweeling Koh - One of the best experts on this subject based on the ideXlab platform.
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antiplatelet and anticoagulant effects of Panax Notoginseng comparison of raw and steamed Panax Notoginseng with Panax ginseng and Panax quinquefolium
Journal of Ethnopharmacology, 2009Co-Authors: Aik Jiang Lau, Dingfung Toh, Tungkian Chua, Yunkeng Pang, Sooon Woo, Hweeling KohAbstract:Abstract Ethnopharmacological significance Panax Notoginseng (Burk.) F. H. Chen (Araliacea) is traditionally used for its hemostatic and cardiovascular effects when raw and as a tonic when steamed. Aim of the study This study aims to compare the effects of raw and steamed Panax Notoginseng, Panax ginseng C. A. Meyer and Panax quinquefolium Linn. on platelet aggregation and plasma coagulation. Materials and methods Effects on collagen-induced platelet aggregation were investigated using a platelet aggregometer, while the plasma coagulation times (prothrombin time, activated partial thromboplastin time and thrombin time) were determined using a blood coagulation analyzer. The data was corroborated with ex vivo platelet aggregation and in vivo rat bleeding time. Results Raw and steamed Panax Notoginseng significantly inhibit platelet aggregation and plasma coagulation. Steamed Panax Notoginseng has significantly more potent antiplatelet and anticoagulant effects than the raw extract, and the antiplatelet and anticoagulant effects increase with increasing steaming durations. Comparing the three common Panax species, Panax Notoginseng has higher antiplatelet effect than Panax ginseng and Panax quinquefolium. The in vitro antiplatelet and anticoagulant effects are positively translated into a prolongation of in vivo rat bleeding time after oral administration of the raw and steamed extracts. Conclusion The results indicate that the three common Panax species affect platelet aggregation and plasma coagulation differently, with steamed Panax Notoginseng showing the greatest antiplatelet and anticoagulant effects. Panax Notoginseng may be a good source of lead compounds for novel antiplatelet and anticoagulant therapeutics.
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ultra performance liquid chromatography time of flight mass spectrometry based metabolomics of raw and steamed Panax Notoginseng
Rapid Communications in Mass Spectrometry, 2007Co-Authors: Eric Chun Yong Chan, Aik Jiang Lau, Dingfung Toh, Sweelee Yap, Paychin Leow, Hweeling KohAbstract:At present, metabolite profiling is of growing importance in herbal medicine fields such as breeding, formulation, quality control and clinical trials. This preliminary study indicated that ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC/TOFMS)-based metabolomics allows direct detection of down-stream derivatives of metabolites, arising from the herbal formulation process. This analytical approach allows the discrimination and tentative authentication of unique biomarkers related to different herbal extracts using unsupervised multivariate principal component analysis (PCA). The tentative identification of biomarkers is complemented significantly by the accurate mass measurement of TOFMS and the high resolution and high retention time reproducibility rendered by UPLC. The application of this approach in herbal extract discrimination and ginsenoside biomarker discovery of raw and steamed Panax Notoginseng (Burk.) F.H. Chen is demonstrated and discussed.
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high performance liquid chromatographic method with quantitative comparisons of whole chromatograms of raw and steamed Panax Notoginseng
Journal of Chromatography A, 2004Co-Authors: Aik Jiang Lau, Sooon Woo, Beehong Seo, Hweeling KohAbstract:A high-performance liquid chromatographic (HPLC) method coupled with chromatographic pattern matching was developed to differentiate whole chromatograms of raw and steamed Panax Notoginseng objectively and quantitatively. The major peaks differentiating chromatograms of raw and steamed samples were also identified for the first time in this herb. The raw and steamed P. Notoginseng roots and its products were successfully differentiated. The quantitative differences between the chromatograms were correlated to the duration of steaming. Chromatographic pattern matching allows rapid, simple, automated, and quantitative comparisons of complex chromatograms. It is a useful tool in ensuring safety and quality of herbal products.
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analysis of saponins in raw and steamed Panax Notoginseng using high performance liquid chromatography with diode array detection
Journal of Chromatography A, 2003Co-Authors: Aik Jiang Lau, Sooon Woo, Hweeling KohAbstract:A reversed-phase high-performance liquid chromatography-diode array detection method was developed and validated for the simultaneous determination of six saponins (notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rc, Rd) in raw and steamed Panax Notoginseng. Linearity (r2 > 0.9988), intra- and inter-day precision (RSD < 4%), limit of detection (0.008-0.013 mg/ml), limit of quantification (0.027-0.042 mg/ml) of the saponins were determined. The method was successfully applied to 11 pairs of raw and steamed P. Notoginseng products. Three products showed discrepancies between theirlabelled claims (raw or steamed) and the results of analysis. This new, simple and reliable method could be used in the quality control of raw and steamed P. Notoginseng.
Aik Jiang Lau - One of the best experts on this subject based on the ideXlab platform.
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antiplatelet and anticoagulant effects of Panax Notoginseng comparison of raw and steamed Panax Notoginseng with Panax ginseng and Panax quinquefolium
Journal of Ethnopharmacology, 2009Co-Authors: Aik Jiang Lau, Dingfung Toh, Tungkian Chua, Yunkeng Pang, Sooon Woo, Hweeling KohAbstract:Abstract Ethnopharmacological significance Panax Notoginseng (Burk.) F. H. Chen (Araliacea) is traditionally used for its hemostatic and cardiovascular effects when raw and as a tonic when steamed. Aim of the study This study aims to compare the effects of raw and steamed Panax Notoginseng, Panax ginseng C. A. Meyer and Panax quinquefolium Linn. on platelet aggregation and plasma coagulation. Materials and methods Effects on collagen-induced platelet aggregation were investigated using a platelet aggregometer, while the plasma coagulation times (prothrombin time, activated partial thromboplastin time and thrombin time) were determined using a blood coagulation analyzer. The data was corroborated with ex vivo platelet aggregation and in vivo rat bleeding time. Results Raw and steamed Panax Notoginseng significantly inhibit platelet aggregation and plasma coagulation. Steamed Panax Notoginseng has significantly more potent antiplatelet and anticoagulant effects than the raw extract, and the antiplatelet and anticoagulant effects increase with increasing steaming durations. Comparing the three common Panax species, Panax Notoginseng has higher antiplatelet effect than Panax ginseng and Panax quinquefolium. The in vitro antiplatelet and anticoagulant effects are positively translated into a prolongation of in vivo rat bleeding time after oral administration of the raw and steamed extracts. Conclusion The results indicate that the three common Panax species affect platelet aggregation and plasma coagulation differently, with steamed Panax Notoginseng showing the greatest antiplatelet and anticoagulant effects. Panax Notoginseng may be a good source of lead compounds for novel antiplatelet and anticoagulant therapeutics.
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ultra performance liquid chromatography time of flight mass spectrometry based metabolomics of raw and steamed Panax Notoginseng
Rapid Communications in Mass Spectrometry, 2007Co-Authors: Eric Chun Yong Chan, Aik Jiang Lau, Dingfung Toh, Sweelee Yap, Paychin Leow, Hweeling KohAbstract:At present, metabolite profiling is of growing importance in herbal medicine fields such as breeding, formulation, quality control and clinical trials. This preliminary study indicated that ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC/TOFMS)-based metabolomics allows direct detection of down-stream derivatives of metabolites, arising from the herbal formulation process. This analytical approach allows the discrimination and tentative authentication of unique biomarkers related to different herbal extracts using unsupervised multivariate principal component analysis (PCA). The tentative identification of biomarkers is complemented significantly by the accurate mass measurement of TOFMS and the high resolution and high retention time reproducibility rendered by UPLC. The application of this approach in herbal extract discrimination and ginsenoside biomarker discovery of raw and steamed Panax Notoginseng (Burk.) F.H. Chen is demonstrated and discussed.
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high performance liquid chromatographic method with quantitative comparisons of whole chromatograms of raw and steamed Panax Notoginseng
Journal of Chromatography A, 2004Co-Authors: Aik Jiang Lau, Sooon Woo, Beehong Seo, Hweeling KohAbstract:A high-performance liquid chromatographic (HPLC) method coupled with chromatographic pattern matching was developed to differentiate whole chromatograms of raw and steamed Panax Notoginseng objectively and quantitatively. The major peaks differentiating chromatograms of raw and steamed samples were also identified for the first time in this herb. The raw and steamed P. Notoginseng roots and its products were successfully differentiated. The quantitative differences between the chromatograms were correlated to the duration of steaming. Chromatographic pattern matching allows rapid, simple, automated, and quantitative comparisons of complex chromatograms. It is a useful tool in ensuring safety and quality of herbal products.
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analysis of saponins in raw and steamed Panax Notoginseng using high performance liquid chromatography with diode array detection
Journal of Chromatography A, 2003Co-Authors: Aik Jiang Lau, Sooon Woo, Hweeling KohAbstract:A reversed-phase high-performance liquid chromatography-diode array detection method was developed and validated for the simultaneous determination of six saponins (notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rc, Rd) in raw and steamed Panax Notoginseng. Linearity (r2 > 0.9988), intra- and inter-day precision (RSD < 4%), limit of detection (0.008-0.013 mg/ml), limit of quantification (0.027-0.042 mg/ml) of the saponins were determined. The method was successfully applied to 11 pairs of raw and steamed P. Notoginseng products. Three products showed discrepancies between theirlabelled claims (raw or steamed) and the results of analysis. This new, simple and reliable method could be used in the quality control of raw and steamed P. Notoginseng.
Fang Liu - One of the best experts on this subject based on the ideXlab platform.
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metabonomics study on the hepatoprotective effect of Panax Notoginseng leaf saponins using uplc q tof ms analysis
The American Journal of Chinese Medicine, 2019Co-Authors: Fang Liu, Meng Wang, Yang Wang, Yiwei Cao, Zhaolin Sun, Mingcang Chen, Xiaoting Tian, Jianbo Wan, Chenggang HuangAbstract:Alcohol liver disease is a major public health problem associated with lifestyle. Our recent study demonstrated that the roots of Panax Notoginseng saponins (PNS) exert hepatoprotective effects against alcohol consumption. Considering that the leaves of Panax Notoginseng saponins (LPNS) have similar chemical ingredients with PNS, increased attention should be given to the hepatoprotective effects of LPNS. In this study, a metabonomic approach based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) was developed to evaluate the hepatoprotective effect of LPNS on alcoholic fatty liver and elucidate the interaction mechanisms. Results showed that the ethanol-induced metabolic perturbations were restored after treatment with LPNS. Furthermore, 12 potential biomarkers (11 upregulated and 1 downregulated) were identified by V-plot and orthogonal partial least square discriminant analysis. Changes in the levels of these metabolites indicated that glycerophospholipid and fatty acid metabolism were disturbed in alcoholic fatty liver mouse. Our findings demonstrated that the UHPLC–QTOF/MS-based metabonomic method may provide a useful means
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metabonomics study on the hepatoprotective effect of Panax Notoginseng leaf saponins using uplc q tof ms analysis
The American Journal of Chinese Medicine, 2019Co-Authors: Fang Liu, Meng Wang, Yang Wang, Yiwei Cao, Zhaolin Sun, Mingcang Chen, Xiaoting Tian, Jianbo Wan, Chenggang HuangAbstract:Alcohol liver disease is a major public health problem associated with lifestyle. Our recent study demonstrated that the roots of Panax Notoginseng saponins (PNS) exert hepatoprotective effects aga...
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Preparative separation of minor saponins from Panax Notoginseng leaves using biotransformation, macroporous resins, and preparative high-performance liquid chromatography
Elsevier, 2019Co-Authors: Fang Liu, Fang-bo Xia, Jianbo WanAbstract:Background: Ginsenosides with less sugar moieties may exhibit the better adsorptive capacity and more pharmacological activities. Methods: An efficient method for the separation of four minor saponins, including gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, from Panax Notoginseng leaves (PNL) was established using biotransformation, macroporous resins, and subsequent preparative high-performance liquid chromatography. Results: The dried PNL powder was immersed in the distilled water at 50°C for 30 min for converting the major saponins, ginsenosides Rb1, Rc, Rb2, and Rb3, to minor saponins, gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, respectively, by the enzymes present in PNL. The adsorption characteristics of these minor saponins on five types of macroporous resins, D-101, DA-201, DM-301, X-5, and S-8, were evaluated and compared. Among them, D-101 was selected due to the best adsorption and desorption properties. Under the optimized conditions, the fraction containing the four target saponins was separated by D-101 resin. Subsequently, the target minor saponins were individually separated and purified by preparative high-performance liquid chromatography with a reversed-phase column. Conclusion: Our study provides a simple and efficient method for the preparation of these four minor saponins from PNL, which will be potential for industrial applications. Keywords: ginsenoside Rd2, gypenoside XVII, notoginsenoside Fd, notoginsenoside Fe, Panax Notoginseng leave
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the antioxidant effects of aqueous and organic extracts of Panax quinquefolium Panax Notoginseng codonopsis pilosula pseudostellaria heterophylla and glehnia littoralis
Journal of Ethnopharmacology, 2004Co-Authors: Fang LiuAbstract:The roots of Panax quinquefolium, Panax Notoginseng, Glehnia littoralis, Codonopsis pilosula and Pseudostellaria heterophylla were extracted with an aqueous extraction method and also with an organic extraction method. The aqueous extracts of Glehnia littoralis and Codonopsis pilosula were the most potent in inhibiting erythrocyte hemolysis. The aqueous extracts of Panax quinquefolium and Panax Notoginseng had lower potencies while the aqueous extract of Pseudostellaria heterophylla and the organic extract of Panax quinquefolium were only weakly active. The organic extracts of Glehnia littoralis, Panax heterophylla and Panax quinquefolium were potent in inhibiting lipid peroxidation while the organic extracts of Codonopsis pilosula and Panax Notoginseng had weaker potencies. The aqueous extracts possessed much lower potencies the corresponding organic extracts. However, the Glehnia littoralis extract was the most potent aqueous extract. The results suggest that Glehnia littoralis, Codonopsis pilosula, Panax Notoginseng and Panax heterophylla are cheaper substitutes of Panax quinquefolium with regard to antioxidant activity.
Jing Rong Wang - One of the best experts on this subject based on the ideXlab platform.
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quantitative comparison and metabolite profiling of saponins in different parts of the root of Panax Notoginseng
Journal of Agricultural and Food Chemistry, 2014Co-Authors: Jing Rong Wang, Zhihong Jiang, Leefong Yau, Weina Gao, Yong Liu, Puiwing Yick, Liang LiuAbstract:Although both rhizome and root of Panax Notoginseng are officially utilized as Notoginseng in “Chinese Pharmacopoeia”, individual parts of the root were differently used in practice. To provide chemical evidence for the differentiated usage, quantitative comparison and metabolite profiling of different portions derived from the whole root, as well as commercial samples, were carried out, showing an overall higher content of saponins in rhizome, followed by main root, branch root, and fibrous root. Ginsenoside Rb2 was proposed as a potential marker with a content of 0.5 mg/g as a threshold value for differentiating rhizome from other parts. Multivariate analysis of the metabolite profile further suggested 32 saponins as potential markers for the discrimination of different parts of Notoginseng. Collectively, the study provided comprehensive chemical evidence for the distinct usage of different parts of Notoginseng and, hence, is of great importance for the rational application and exploitation of individua...
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dammarane type triterpene saponins from the flowers of Panax Notoginseng
Molecules, 2009Co-Authors: Jing Rong Wang, Yuko Yamasaki, Takashi Tanaka, Isao Kouno, Zhihong JiangAbstract:Four new dammarane-type triterpene saponins named floranotoginsenosides A (1), B (2), C (3) and D (4), together with five known triterpene saponins, were isolated from the flowers of Panax Notoginseng. Their structures were elucidated on the basis of spectral and chemical evidence.
