The Experts below are selected from a list of 3282 Experts worldwide ranked by ideXlab platform
Zhihe Zhang - One of the best experts on this subject based on the ideXlab platform.
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twelve polymorphic microsatellite loci for the south china tiger Panthera Tigris amoyensis
Molecular Ecology Notes, 2006Co-Authors: Zhihe Zhang, Wenping Zhang, Fujun Shen, L ZhangAbstract:Twelve microsatellite loci were found from genomic DNA-enriched libraries of the South China tiger (Panthera Tigris amoyensis). The number of observed alleles for each locus in 53 individuals ranged from five to 14; the expected and observed heterozygosity was 0.480 to 0.883 and 0.333 to 0.956, respectively; and the mean polymorphic information content (PIC) was 0.729. These markers would be considered a useful tool for the study of genetic variation in South China tiger in the future.
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Sequence variation of mitochondrial DNA ND5 in captive South China tigers (Panthera Tigris amoyensis)
Journal of Natural History, 2005Co-Authors: Zhihe Zhang, Wenping Zhang, Fujun Shen, Guangxin HeAbstract:This paper represents the first study of the genetic diversity of captive South China tigers Panthera Tigris amoyensis by sequence variation in a fragment of mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (ND5) gene. Two haplotypes (haplotype I and haplotype II) were identified and the captive population had a bias towards haplotype I. This subspecies as a whole demonstrated an extremely low level of genetic diversity with the nucleotide diversity of 0.057±0.021%. The mtDNA ND5 variations detected in the present study could provide significant information to the studbook data of the South China tiger.
L Zhang - One of the best experts on this subject based on the ideXlab platform.
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twelve polymorphic microsatellite loci for the south china tiger Panthera Tigris amoyensis
Molecular Ecology Notes, 2006Co-Authors: Zhihe Zhang, Wenping Zhang, Fujun Shen, L ZhangAbstract:Twelve microsatellite loci were found from genomic DNA-enriched libraries of the South China tiger (Panthera Tigris amoyensis). The number of observed alleles for each locus in 53 individuals ranged from five to 14; the expected and observed heterozygosity was 0.480 to 0.883 and 0.333 to 0.956, respectively; and the mean polymorphic information content (PIC) was 0.729. These markers would be considered a useful tool for the study of genetic variation in South China tiger in the future.
Robert Barber - One of the best experts on this subject based on the ideXlab platform.
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development and cross species amplification of 18 microsatellite markers in the sumatran tiger Panthera Tigris sumatrae
Molecular Ecology Notes, 2002Co-Authors: Jennifer Williamson, Ryan M Huebinger, Julie Sommer, Edward E Louis, Robert BarberAbstract:Eighteen polymorphic microsatellite loci from the highly endangered Sumatran tiger (Panthera Tigris sumatrae) were isolated and characterized. Upon polymerase chain reaction amplification, 16 of these markers produced a single, sharp band in all three tiger and 10 non-tiger felid species examined. Of the two remaining loci, 6HDZ057 and 6HDZ635 failed to amplify genomic DNA from puma (Felis concolor) and cheetah (Acinonyx jubatus), respectively. The amplification of these markers across four genera is an indication of their usefulness for population genetics studies and conservation work in a wide range of felid species.
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Development and cross‐species amplification of 18 microsatellite markers in the Sumatran tiger (Panthera Tigris sumatrae)
Molecular Ecology Notes, 2002Co-Authors: Jennifer Williamson, Ryan M Huebinger, Edward E Louis, Julie A. Sommer, Robert BarberAbstract:Eighteen polymorphic microsatellite loci from the highly endangered Sumatran tiger (Panthera Tigris sumatrae) were isolated and characterized. Upon polymerase chain reaction amplification, 16 of these markers produced a single, sharp band in all three tiger and 10 non-tiger felid species examined. Of the two remaining loci, 6HDZ057 and 6HDZ635 failed to amplify genomic DNA from puma (Felis concolor) and cheetah (Acinonyx jubatus), respectively. The amplification of these markers across four genera is an indication of their usefulness for population genetics studies and conservation work in a wide range of felid species.
Fujun Shen - One of the best experts on this subject based on the ideXlab platform.
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twelve polymorphic microsatellite loci for the south china tiger Panthera Tigris amoyensis
Molecular Ecology Notes, 2006Co-Authors: Zhihe Zhang, Wenping Zhang, Fujun Shen, L ZhangAbstract:Twelve microsatellite loci were found from genomic DNA-enriched libraries of the South China tiger (Panthera Tigris amoyensis). The number of observed alleles for each locus in 53 individuals ranged from five to 14; the expected and observed heterozygosity was 0.480 to 0.883 and 0.333 to 0.956, respectively; and the mean polymorphic information content (PIC) was 0.729. These markers would be considered a useful tool for the study of genetic variation in South China tiger in the future.
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Sequence variation of mitochondrial DNA ND5 in captive South China tigers (Panthera Tigris amoyensis)
Journal of Natural History, 2005Co-Authors: Zhihe Zhang, Wenping Zhang, Fujun Shen, Guangxin HeAbstract:This paper represents the first study of the genetic diversity of captive South China tigers Panthera Tigris amoyensis by sequence variation in a fragment of mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (ND5) gene. Two haplotypes (haplotype I and haplotype II) were identified and the captive population had a bias towards haplotype I. This subspecies as a whole demonstrated an extremely low level of genetic diversity with the nucleotide diversity of 0.057±0.021%. The mtDNA ND5 variations detected in the present study could provide significant information to the studbook data of the South China tiger.
Wenping Zhang - One of the best experts on this subject based on the ideXlab platform.
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twelve polymorphic microsatellite loci for the south china tiger Panthera Tigris amoyensis
Molecular Ecology Notes, 2006Co-Authors: Zhihe Zhang, Wenping Zhang, Fujun Shen, L ZhangAbstract:Twelve microsatellite loci were found from genomic DNA-enriched libraries of the South China tiger (Panthera Tigris amoyensis). The number of observed alleles for each locus in 53 individuals ranged from five to 14; the expected and observed heterozygosity was 0.480 to 0.883 and 0.333 to 0.956, respectively; and the mean polymorphic information content (PIC) was 0.729. These markers would be considered a useful tool for the study of genetic variation in South China tiger in the future.
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Sequence variation of mitochondrial DNA ND5 in captive South China tigers (Panthera Tigris amoyensis)
Journal of Natural History, 2005Co-Authors: Zhihe Zhang, Wenping Zhang, Fujun Shen, Guangxin HeAbstract:This paper represents the first study of the genetic diversity of captive South China tigers Panthera Tigris amoyensis by sequence variation in a fragment of mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (ND5) gene. Two haplotypes (haplotype I and haplotype II) were identified and the captive population had a bias towards haplotype I. This subspecies as a whole demonstrated an extremely low level of genetic diversity with the nucleotide diversity of 0.057±0.021%. The mtDNA ND5 variations detected in the present study could provide significant information to the studbook data of the South China tiger.
