The Experts below are selected from a list of 6501 Experts worldwide ranked by ideXlab platform
Lou-sing Kan - One of the best experts on this subject based on the ideXlab platform.
-
Paper Clip type triple helix formation by 5 d tc 3ta ct 3cb ag 3 a and b 0 4 as a function of loop size with and without the pseudoisocytosine base in the hoogsteen strand
Biochemistry, 2000Co-Authors: Tsung-mei Chin, Shwu-bin Lin, Szu-yin Lee, Mu-li Chang, Alice Yu-yu Cheng, Fu-chao Chang, Laura B. Pasternack, Dee-hua Huang, Lou-sing KanAbstract:The formation of a DNA “Paper-Clip” type triple helix (triplex) with a common sequence 5‘-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0−4) was studied by UV thermal melting experiments and CD spectra. These DNA oligomers form triplexes and duplexes under slightly acidic and neutral conditions, respectively. The stability of the formed triplexes (at pH 4.5) or duplexes (at pH 7.0 or 8.0) does not vary significantly with the size of the loops (a and b = 1−4). At pH 6.0, the triplex stability is, however, a function of a and b. It is also interesting to note that the oligomer 5‘-d-(TC)3(CT)3(AG)3 (a and b = 0) forms a stable triplex at pH 4.5 with a slightly lower Tm value, due to dissociation of a base triad at one end and a distorted base triad at the other, observed by 1H NMR. Thus, we have here a model system, 5‘-d-(TC)3Ta(CT)3Cb(AG)3, that could form a triplex effectively with (a and b = 1−4) and without (a and b = 0) loops under acidic conditions. In addition, the triplex formation of oligomers with replacement o...
-
Paper-Clip" type triple helix formation by 5'-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0-4) as a function of loop size with and without the pseudoisocytosine base in the Hoogsteen strand.
Biochemistry, 2000Co-Authors: Tsung-mei Chin, Shwu-bin Lin, Szu-yin Lee, Mu-li Chang, Alice Yu-yu Cheng, Fu-chao Chang, Laura B. Pasternack, Dee-hua Huang, Lou-sing KanAbstract:The formation of a DNA “Paper-Clip” type triple helix (triplex) with a common sequence 5‘-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0−4) was studied by UV thermal melting experiments and CD spectra. These DNA oligomers form triplexes and duplexes under slightly acidic and neutral conditions, respectively. The stability of the formed triplexes (at pH 4.5) or duplexes (at pH 7.0 or 8.0) does not vary significantly with the size of the loops (a and b = 1−4). At pH 6.0, the triplex stability is, however, a function of a and b. It is also interesting to note that the oligomer 5‘-d-(TC)3(CT)3(AG)3 (a and b = 0) forms a stable triplex at pH 4.5 with a slightly lower Tm value, due to dissociation of a base triad at one end and a distorted base triad at the other, observed by 1H NMR. Thus, we have here a model system, 5‘-d-(TC)3Ta(CT)3Cb(AG)3, that could form a triplex effectively with (a and b = 1−4) and without (a and b = 0) loops under acidic conditions. In addition, the triplex formation of oligomers with replacement o...
Tsung-mei Chin - One of the best experts on this subject based on the ideXlab platform.
-
Paper Clip type triple helix formation by 5 d tc 3ta ct 3cb ag 3 a and b 0 4 as a function of loop size with and without the pseudoisocytosine base in the hoogsteen strand
Biochemistry, 2000Co-Authors: Tsung-mei Chin, Shwu-bin Lin, Szu-yin Lee, Mu-li Chang, Alice Yu-yu Cheng, Fu-chao Chang, Laura B. Pasternack, Dee-hua Huang, Lou-sing KanAbstract:The formation of a DNA “Paper-Clip” type triple helix (triplex) with a common sequence 5‘-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0−4) was studied by UV thermal melting experiments and CD spectra. These DNA oligomers form triplexes and duplexes under slightly acidic and neutral conditions, respectively. The stability of the formed triplexes (at pH 4.5) or duplexes (at pH 7.0 or 8.0) does not vary significantly with the size of the loops (a and b = 1−4). At pH 6.0, the triplex stability is, however, a function of a and b. It is also interesting to note that the oligomer 5‘-d-(TC)3(CT)3(AG)3 (a and b = 0) forms a stable triplex at pH 4.5 with a slightly lower Tm value, due to dissociation of a base triad at one end and a distorted base triad at the other, observed by 1H NMR. Thus, we have here a model system, 5‘-d-(TC)3Ta(CT)3Cb(AG)3, that could form a triplex effectively with (a and b = 1−4) and without (a and b = 0) loops under acidic conditions. In addition, the triplex formation of oligomers with replacement o...
-
Paper-Clip" type triple helix formation by 5'-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0-4) as a function of loop size with and without the pseudoisocytosine base in the Hoogsteen strand.
Biochemistry, 2000Co-Authors: Tsung-mei Chin, Shwu-bin Lin, Szu-yin Lee, Mu-li Chang, Alice Yu-yu Cheng, Fu-chao Chang, Laura B. Pasternack, Dee-hua Huang, Lou-sing KanAbstract:The formation of a DNA “Paper-Clip” type triple helix (triplex) with a common sequence 5‘-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0−4) was studied by UV thermal melting experiments and CD spectra. These DNA oligomers form triplexes and duplexes under slightly acidic and neutral conditions, respectively. The stability of the formed triplexes (at pH 4.5) or duplexes (at pH 7.0 or 8.0) does not vary significantly with the size of the loops (a and b = 1−4). At pH 6.0, the triplex stability is, however, a function of a and b. It is also interesting to note that the oligomer 5‘-d-(TC)3(CT)3(AG)3 (a and b = 0) forms a stable triplex at pH 4.5 with a slightly lower Tm value, due to dissociation of a base triad at one end and a distorted base triad at the other, observed by 1H NMR. Thus, we have here a model system, 5‘-d-(TC)3Ta(CT)3Cb(AG)3, that could form a triplex effectively with (a and b = 1−4) and without (a and b = 0) loops under acidic conditions. In addition, the triplex formation of oligomers with replacement o...
Warren Schubert - One of the best experts on this subject based on the ideXlab platform.
-
A Calibrated Paper Clip Is a Reliable Measure of Two‐point Discrimination
Academic Emergency Medicine, 2004Co-Authors: John T. Finnell, Robert K Knopp, Phelps Johnson, Patrick C. Holland, Warren SchubertAbstract:Objectives: The primary objective of this study was to compare two different instruments for assessing digital nerve function; a secondary aim was to determine interobserver agreement among emergency physicians by using static two-point testing of digital nerve function. Methods: This was a prospective, blinded, observational study of static two-point discrimination involving healthy volunteers aged 18-59 years. The authors compared two instruments (Paper Clip set or Disk-Criminator) to assess two-point discrimination of the index and long fingers of the dominant hand. For each subject, the initial investigator and initial testing instrument were randomized. Two-point testing was conducted at 4, 5, and 6 mm by using six randomly selected stimuli (1 or 2 points) for each distal phalanx tested. The study was designed to detect a 25% difference in mean two-point distance with a power of 80%. Results: Seventy-five subjects were entered into the study, of which two were excluded. Interinstrument agreement for a given investigator ranged from 77% to 84% for absolute agreement and 98% to 100% within 2 mm. Weighted kappa values for interobserver differences of 2 mm or less was 0.79 to 1.00. There was no statistically significant difference between instruments. Conclusions: Using a clinically relevant threshold of 2 mm, the authors found that a properly calibrated set of Paper Clips performed as well as the Disk-Criminator.
-
a calibrated Paper Clip is a reliable measure of two point discrimination
Academic Emergency Medicine, 2004Co-Authors: John T. Finnell, Robert K Knopp, Phelps Johnson, Patrick C. Holland, Warren SchubertAbstract:Objectives: The primary objective of this study was to compare two different instruments for assessing digital nerve function; a secondary aim was to determine interobserver agreement among emergency physicians by using static two-point testing of digital nerve function. Methods: This was a prospective, blinded, observational study of static two-point discrimination involving healthy volunteers aged 18-59 years. The authors compared two instruments (Paper Clip set or Disk-Criminator) to assess two-point discrimination of the index and long fingers of the dominant hand. For each subject, the initial investigator and initial testing instrument were randomized. Two-point testing was conducted at 4, 5, and 6 mm by using six randomly selected stimuli (1 or 2 points) for each distal phalanx tested. The study was designed to detect a 25% difference in mean two-point distance with a power of 80%. Results: Seventy-five subjects were entered into the study, of which two were excluded. Interinstrument agreement for a given investigator ranged from 77% to 84% for absolute agreement and 98% to 100% within 2 mm. Weighted kappa values for interobserver differences of 2 mm or less was 0.79 to 1.00. There was no statistically significant difference between instruments. Conclusions: Using a clinically relevant threshold of 2 mm, the authors found that a properly calibrated set of Paper Clips performed as well as the Disk-Criminator.
Dee-hua Huang - One of the best experts on this subject based on the ideXlab platform.
-
Paper Clip type triple helix formation by 5 d tc 3ta ct 3cb ag 3 a and b 0 4 as a function of loop size with and without the pseudoisocytosine base in the hoogsteen strand
Biochemistry, 2000Co-Authors: Tsung-mei Chin, Shwu-bin Lin, Szu-yin Lee, Mu-li Chang, Alice Yu-yu Cheng, Fu-chao Chang, Laura B. Pasternack, Dee-hua Huang, Lou-sing KanAbstract:The formation of a DNA “Paper-Clip” type triple helix (triplex) with a common sequence 5‘-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0−4) was studied by UV thermal melting experiments and CD spectra. These DNA oligomers form triplexes and duplexes under slightly acidic and neutral conditions, respectively. The stability of the formed triplexes (at pH 4.5) or duplexes (at pH 7.0 or 8.0) does not vary significantly with the size of the loops (a and b = 1−4). At pH 6.0, the triplex stability is, however, a function of a and b. It is also interesting to note that the oligomer 5‘-d-(TC)3(CT)3(AG)3 (a and b = 0) forms a stable triplex at pH 4.5 with a slightly lower Tm value, due to dissociation of a base triad at one end and a distorted base triad at the other, observed by 1H NMR. Thus, we have here a model system, 5‘-d-(TC)3Ta(CT)3Cb(AG)3, that could form a triplex effectively with (a and b = 1−4) and without (a and b = 0) loops under acidic conditions. In addition, the triplex formation of oligomers with replacement o...
-
Paper-Clip" type triple helix formation by 5'-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0-4) as a function of loop size with and without the pseudoisocytosine base in the Hoogsteen strand.
Biochemistry, 2000Co-Authors: Tsung-mei Chin, Shwu-bin Lin, Szu-yin Lee, Mu-li Chang, Alice Yu-yu Cheng, Fu-chao Chang, Laura B. Pasternack, Dee-hua Huang, Lou-sing KanAbstract:The formation of a DNA “Paper-Clip” type triple helix (triplex) with a common sequence 5‘-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0−4) was studied by UV thermal melting experiments and CD spectra. These DNA oligomers form triplexes and duplexes under slightly acidic and neutral conditions, respectively. The stability of the formed triplexes (at pH 4.5) or duplexes (at pH 7.0 or 8.0) does not vary significantly with the size of the loops (a and b = 1−4). At pH 6.0, the triplex stability is, however, a function of a and b. It is also interesting to note that the oligomer 5‘-d-(TC)3(CT)3(AG)3 (a and b = 0) forms a stable triplex at pH 4.5 with a slightly lower Tm value, due to dissociation of a base triad at one end and a distorted base triad at the other, observed by 1H NMR. Thus, we have here a model system, 5‘-d-(TC)3Ta(CT)3Cb(AG)3, that could form a triplex effectively with (a and b = 1−4) and without (a and b = 0) loops under acidic conditions. In addition, the triplex formation of oligomers with replacement o...
Laura B. Pasternack - One of the best experts on this subject based on the ideXlab platform.
-
Paper Clip type triple helix formation by 5 d tc 3ta ct 3cb ag 3 a and b 0 4 as a function of loop size with and without the pseudoisocytosine base in the hoogsteen strand
Biochemistry, 2000Co-Authors: Tsung-mei Chin, Shwu-bin Lin, Szu-yin Lee, Mu-li Chang, Alice Yu-yu Cheng, Fu-chao Chang, Laura B. Pasternack, Dee-hua Huang, Lou-sing KanAbstract:The formation of a DNA “Paper-Clip” type triple helix (triplex) with a common sequence 5‘-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0−4) was studied by UV thermal melting experiments and CD spectra. These DNA oligomers form triplexes and duplexes under slightly acidic and neutral conditions, respectively. The stability of the formed triplexes (at pH 4.5) or duplexes (at pH 7.0 or 8.0) does not vary significantly with the size of the loops (a and b = 1−4). At pH 6.0, the triplex stability is, however, a function of a and b. It is also interesting to note that the oligomer 5‘-d-(TC)3(CT)3(AG)3 (a and b = 0) forms a stable triplex at pH 4.5 with a slightly lower Tm value, due to dissociation of a base triad at one end and a distorted base triad at the other, observed by 1H NMR. Thus, we have here a model system, 5‘-d-(TC)3Ta(CT)3Cb(AG)3, that could form a triplex effectively with (a and b = 1−4) and without (a and b = 0) loops under acidic conditions. In addition, the triplex formation of oligomers with replacement o...
-
Paper-Clip" type triple helix formation by 5'-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0-4) as a function of loop size with and without the pseudoisocytosine base in the Hoogsteen strand.
Biochemistry, 2000Co-Authors: Tsung-mei Chin, Shwu-bin Lin, Szu-yin Lee, Mu-li Chang, Alice Yu-yu Cheng, Fu-chao Chang, Laura B. Pasternack, Dee-hua Huang, Lou-sing KanAbstract:The formation of a DNA “Paper-Clip” type triple helix (triplex) with a common sequence 5‘-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0−4) was studied by UV thermal melting experiments and CD spectra. These DNA oligomers form triplexes and duplexes under slightly acidic and neutral conditions, respectively. The stability of the formed triplexes (at pH 4.5) or duplexes (at pH 7.0 or 8.0) does not vary significantly with the size of the loops (a and b = 1−4). At pH 6.0, the triplex stability is, however, a function of a and b. It is also interesting to note that the oligomer 5‘-d-(TC)3(CT)3(AG)3 (a and b = 0) forms a stable triplex at pH 4.5 with a slightly lower Tm value, due to dissociation of a base triad at one end and a distorted base triad at the other, observed by 1H NMR. Thus, we have here a model system, 5‘-d-(TC)3Ta(CT)3Cb(AG)3, that could form a triplex effectively with (a and b = 1−4) and without (a and b = 0) loops under acidic conditions. In addition, the triplex formation of oligomers with replacement o...
